{"entity": "researcher", "timestamp": "2026-08-20T20:47:30.596Z", "family": "Buijs", "given": "Jos", "initials": "J", "orcid": "0000-0003-3955-1320", "affiliations": ["Ridgeview Instruments AB, Uppsala, Sweden.", "Department of Immunology, Pathology and Genetics, Rudbeck Laboratory, Uppsala University, Uppsala, Sweden."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/b8ab0f168ed348baa89d0578fc9c78a1.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/b8ab0f168ed348baa89d0578fc9c78a1"}}, "publications": [{"entity": "publication", "iuid": "bdf38c665d3a41b7914386d271c5fbaf", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/bdf38c665d3a41b7914386d271c5fbaf.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/bdf38c665d3a41b7914386d271c5fbaf"}}, "title": "Deciphering the RNA recognition by Musashi-1 to design protein and RNA variants for in vitro and in vivo applications.", "authors": [{"family": "P\u00e9rez-R\u00e0fols", "given": "Anna", "initials": "A", "orcid": "0000-0001-7492-2583", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5a6fa8b62a9d4bcaa73ea3e19256b533.json"}}, {"family": "P\u00e9rez-Ropero", "given": "Guillermo", "initials": "G", "orcid": "0000-0001-7595-4851", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3ab708040fd948028309564a02b365d1.json"}}, {"family": "Cerofolini", "given": "Linda", "initials": "L", "orcid": "0000-0002-0795-9594", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3890e72a89e34346ad9646f8033f4b11.json"}}, {"family": "Sperotto", "given": "Luca", "initials": "L", "orcid": "0009-0007-0780-4991", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d70a5c607e9a452f93473cfc15c97892.json"}}, {"family": "Roca-Mart\u00ednez", "given": "Joel", "initials": "J", "orcid": "0000-0002-4313-3845", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/eea6ba4d722e48dc857dd319e1a5d153.json"}}, {"family": "Higuera-Rodr\u00edguez", "given": "R Anah\u00ed", "initials": "RA", "orcid": "0000-0002-4170-8799", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/2143580b43494522b12fb40abbf6bca2.json"}}, {"family": "Russomanno", "given": "Pasquale", "initials": "P", "orcid": "0000-0002-0236-1200", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6210a7cfb2d64eb5b5753d2e7df756cf.json"}}, {"family": "Kaiser", "given": "Wolfgang", "initials": "W"}, {"family": "Vranken", "given": "Wim", "initials": "W", "orcid": "0000-0001-7470-4324", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8042d76fe5e648cf93b2da58170862d8.json"}}, {"family": "Danielson", "given": "U Helena", "initials": "UH", "orcid": "0000-0003-2728-0340", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c1be15d9a1ea49e1b0e7bb712522696e.json"}}, {"family": "Provenzani", "given": "Alessandro", "initials": "A", "orcid": "0000-0003-1652-3415", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/55b67b395d594bf9adf0bce9b666752a.json"}}, {"family": "Martelli", "given": "Tommaso", "initials": "T", "orcid": "0000-0003-2394-818X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1cdad646b611434b8af781faf892964d.json"}}, {"family": "Sattler", "given": "Michael", "initials": "M", "orcid": "0000-0002-1594-0527", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f0486fc83d394547a4c4da28d0a3f3c9.json"}}, {"family": "Buijs", "given": "Jos", "initials": "J", "orcid": "0000-0003-3955-1320", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b8ab0f168ed348baa89d0578fc9c78a1.json"}}, {"family": "Fragai", "given": "Marco", "initials": "M", "orcid": "0000-0002-8440-1690", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/631de2ca44c64b8685eae11323d4d722.json"}}], "type": "journal article", "published": "2025-08-11", "journal": {"title": "Nucleic Acids Res.", "issn": "1362-4962", "volume": "53", "issue": "15", "issn-l": "0305-1048"}, "abstract": "The Human Musashi-1 (MSI-1) is an RNA-binding protein that recognizes (G/A)U1-3AGU and UAG sequences in diverse RNAs through two RNA Recognition Motif (RRM) domains and regulates the fate of target RNA. Here, we have combined structural biology and computational approaches to analyse the binding of the RRM domains of human MSI-1 with single-stranded and structured RNA ligands. We have used our recently developed computational tool RRMScorer to design a set of substitutions in the MSI-1 protein and the investigated RNA strands to modulate the binding affinity and selectivity. The in silico predictions of the designed protein-RNA interactions are assessed by nuclear magnetic resonance and surface plasmon resonance. These experiments have also been used to study the competition of the two RRM domains of MSI-1 for the same binding site within linear and harpin RNA. Our experimental results shed light on MSI-RNA interactions, thus opening the way for the development of new biomolecules for in vitro and in vivo studies and downstream applications.", "doi": "10.1093/nar/gkaf741", "pmid": "40795964", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC12342889"}, {"db": "pii", "key": "8230323"}], "notes": [], "created": "2026-08-20T09:50:21.629Z", "modified": "2026-08-20T09:50:22.096Z"}, {"entity": "publication", "iuid": "21b2fba629b34393bd8e2054d8099d6c", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/21b2fba629b34393bd8e2054d8099d6c.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/21b2fba629b34393bd8e2054d8099d6c"}}, "title": "Regulatory Effects of RNA-Protein Interactions Revealed by Reporter Assays of Bacteria Grown on Solid Media.", "authors": [{"family": "P\u00e9rez-Ropero", "given": "Guillermo", "initials": "G", "orcid": "0000-0001-7595-4851", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3ab708040fd948028309564a02b365d1.json"}}, {"family": "Dolcemascolo", "given": "Roswitha", "initials": "R", "orcid": "0000-0003-2851-7194", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b7bc348a827a4e6ab3ff82f1e2c5925b.json"}}, {"family": "P\u00e9rez-R\u00e0fols", "given": "Anna", "initials": "A", "orcid": "0000-0001-7492-2583", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5a6fa8b62a9d4bcaa73ea3e19256b533.json"}}, {"family": "Andersson", "given": "Karl", "initials": "K", "orcid": "0000-0001-9141-9242", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3c2790814ce34e97b517ace827470e6d.json"}}, {"family": "Danielson", "given": "U Helena", "initials": "UH", "orcid": "0000-0003-2728-0340", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c1be15d9a1ea49e1b0e7bb712522696e.json"}}, {"family": "Rodrigo", "given": "Guillermo", "initials": "G", "orcid": "0000-0002-1871-9617", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/194cb38da72a4278a5a84ea87580f288.json"}}, {"family": "Buijs", "given": "Jos", "initials": "J", "orcid": "0000-0003-3955-1320", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b8ab0f168ed348baa89d0578fc9c78a1.json"}}], "type": "journal article", "published": "2025-03-08", "journal": {"title": "Biosensors (Basel)", "issn": "2079-6374", "volume": "15", "issue": "3", "issn-l": null}, "abstract": "Reporter systems are widely used to study biomolecular interactions and processes in vivo, representing one of the basic tools used to characterize synthetic regulatory circuits. Here, we developed a method that enables the monitoring of RNA-protein interactions through a reporter system in bacteria with high temporal resolution. For this, we used a Real-Time Protein Expression Assay (RT-PEA) technology for real-time monitoring of a fluorescent reporter protein, while having bacteria growing on solid media. Experimental results were analyzed by fitting a three-variable Gompertz growth model. To validate the method, the interactions between a set of RNA sequences and the RNA-binding protein (RBP) Musashi-1 (MSI1) were evaluated, as well as the allosteric modulation of the interaction by a small molecule (oleic acid). This new approach proved to be suitable to quantitatively characterize RNA-RBP interactions, thereby expanding the toolbox to study molecular interactions in living bacteria, including allosteric modulation, with special relevance for systems that are not suitable to be studied in liquid media.", "doi": "10.3390/bios15030175", "pmid": "40136972", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC11940492"}, {"db": "pii", "key": "bios15030175"}], "notes": [], "created": "2026-08-20T13:39:06.008Z", "modified": "2026-08-20T13:39:06.236Z"}, {"entity": "publication", "iuid": "c9ac4b128dae4490affaa8e236227243", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/c9ac4b128dae4490affaa8e236227243.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/c9ac4b128dae4490affaa8e236227243"}}, "title": "A real-time cell-binding assay reveals dynamic features of STxB-Gb3 cointernalization and STxB-mediated cargo delivery into cancer cells.", "authors": [{"family": "Encarna\u00e7\u00e3o", "given": "Jo\u00e3o Crispim", "initials": "JC", "orcid": "0000-0002-4509-4106", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/200b4d7ba0e948a6afe685b5d18447bc.json"}}, {"family": "Napolitano", "given": "Valeria", "initials": "V"}, {"family": "Opassi", "given": "Giulia", "initials": "G", "orcid": "0000-0003-0177-537X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b0e27076387447b1a9946f3d4d419292.json"}}, {"family": "Danielson", "given": "U Helena", "initials": "UH"}, {"family": "Dubin", "given": "Grzegorz", "initials": "G"}, {"family": "Popowicz", "given": "Grzegorz M", "initials": "GM"}, {"family": "Munier-Lehmann", "given": "H\u00e9l\u00e8ne", "initials": "H"}, {"family": "Buijs", "given": "Jos", "initials": "J", "orcid": "0000-0003-3955-1320", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b8ab0f168ed348baa89d0578fc9c78a1.json"}}, {"family": "Andersson", "given": "Karl", "initials": "K"}, {"family": "Bj\u00f6rkelund", "given": "Hanna", "initials": "H"}], "type": "journal article", "published": "2020-08-00", "journal": {"title": "FEBS Lett.", "issn": "1873-3468", "volume": "594", "issue": "15", "pages": "2406-2420", "issn-l": "0014-5793"}, "abstract": "The interaction between the Shiga toxin B-subunit (STxB) and its globotriaosylceramide receptor (Gb3) has a high potential for being exploited for targeted cancer therapy. The primary goal of this study was to evaluate the capacity of STxB to carry small molecules and proteins as cargo into cells. For this purpose, an assay was designed to provide real-time information about the StxB-Gb3 interaction as well as the dynamics and mechanism of the internalization process. The assay revealed the ability to distinguish the process of binding to the cell surface from internalization and presented the importance of receptor and STxB clustering for internalization. The overall setup demonstrated that the binding mechanism is complex, and the concept of affinity is difficult to apply. Hence, time-resolved methods, providing detailed information about the interaction of STxB with cells, are critical for the optimization of intracellular delivery.", "doi": "10.1002/1873-3468.13847", "pmid": "32473599", "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T06:25:21.172Z", "modified": "2026-08-20T06:25:21.540Z"}, {"entity": "publication", "iuid": "5df0f2a0723d4f13926d55490984aa52", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/5df0f2a0723d4f13926d55490984aa52.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/5df0f2a0723d4f13926d55490984aa52"}}, "title": "Comparative evaluation of tumor targeting using the anti-HER2 ADAPT scaffold protein labeled at the C-terminus with indium-111 or technetium-99m.", "authors": [{"family": "Garousi", "given": "Javad", "initials": "J"}, {"family": "Lindbo", "given": "Sarah", "initials": "S"}, {"family": "Mitran", "given": "Bogdan", "initials": "B"}, {"family": "Buijs", "given": "Jos", "initials": "J", "orcid": "0000-0003-3955-1320", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b8ab0f168ed348baa89d0578fc9c78a1.json"}}, {"family": "Vorobyeva", "given": "Anzhelika", "initials": "A"}, {"family": "Orlova", "given": "Anna", "initials": "A"}, {"family": "Tolmachev", "given": "Vladimir", "initials": "V"}, {"family": "Hober", "given": "Sophia", "initials": "S"}], "type": "comparative study", "published": "2017-11-07", "journal": {"title": "Sci Rep", "issn": "2045-2322", "volume": "7", "issue": "1", "pages": "14780", "issn-l": "2045-2322"}, "abstract": "ABD-Derived Affinity Proteins (ADAPTs) is a novel class of engineered scaffold proteins derived from an albumin-binding domain of protein G. The use of ADAPT6 derivatives as targeting moiety have provided excellent preclinical radionuclide imaging of human epidermal growth factor 2 (HER2) tumor xenografts. Previous studies have demonstrated that selection of nuclide and chelator for its conjugation has an appreciable effect on imaging properties of scaffold proteins. In this study we performed a comparative evaluation of the anti-HER2 ADAPT having an aspartate-glutamate-alanine-valine-aspartate-alanine-asparagine-serine (DEAVDANS) N-terminal sequence and labeled at C-terminus with 99mTc using a cysteine-containing peptide based chelator, glycine-serine-serine-cysteine (GSSC), and a similar variant labeled with 111In using a maleimido derivative of 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) chelator. Both 99mTc-DEAVDANS-ADAPT6-GSSC and 111In-DEAVDANS-ADAPT6-GSSC-DOTA accumulated specifically in HER2-expressing SKOV3 xenografts. The tumor uptake of both variants did not differ significantly and average values were in the range of 19-21%ID/g. However, there was an appreciable variation in uptake of conjugates in normal tissues that resulted in a notable difference in the tumor-to-organ ratios. The 111In-DOTA label provided 2-6 fold higher tumor-to-organ ratios than 99mTc-GSSC and is therefore the preferable label for ADAPTs.", "doi": "10.1038/s41598-017-15366-w", "pmid": "29116215", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC5676751"}, {"db": "pii", "key": "10.1038/s41598-017-15366-w"}], "notes": [], "created": "2026-08-20T09:04:17.421Z", "modified": "2026-08-20T09:04:17.442Z"}]}