{"entity": "researcher", "timestamp": "2026-08-20T20:36:41.697Z", "family": "Lindblad", "given": "Caroline", "initials": "C", "orcid": "0000-0003-4952-8597", "affiliations": ["Division of Neurosurgery, Department of Clinical Neurosciences, University of Cambridge, Cambridge, United Kingdom.", "Section for Neurosurgery, Department of Medical Sciences, Uppsala University, Uppsala, Sweden.", "Department of Clinical Neuroscience, Karolinska Institutet, Stockholm, Sweden."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/b3fe10d5ae0a4812a87c1d416201d408.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/b3fe10d5ae0a4812a87c1d416201d408"}}, "publications": [{"entity": "publication", "iuid": "d8403987609040cebaa5bbccc48f0983", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/d8403987609040cebaa5bbccc48f0983.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/d8403987609040cebaa5bbccc48f0983"}}, "title": "Inflammatory Mediators Related to Vascular Dysfunction are Linked to ICP, PRx, and CPP Following Human Severe Traumatic Brain Injury.", "authors": [{"family": "Smith", "given": "Claudia Ann", "initials": "CA", "orcid": "0000-0003-3643-6191", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/00caa1ee179346f8960c6ff3a1a3f496.json"}}, {"family": "Lindblad", "given": "Caroline", "initials": "C", "orcid": "0000-0003-4952-8597", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b3fe10d5ae0a4812a87c1d416201d408.json"}}, {"family": "Needham", "given": "Edward", "initials": "E", "orcid": "0000-0001-7042-7462", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8ee0e6f48b4d40bd8301eec6401979bf.json"}}, {"family": "Beqiri", "given": "Erta", "initials": "E"}, {"family": "Bergstr\u00f6m", "given": "Sofia", "initials": "S"}, {"family": "Smielewski", "given": "Peter", "initials": "P"}, {"family": "Nilsson", "given": "Peter", "initials": "P"}, {"family": "Thelin", "given": "Eric", "initials": "E", "orcid": "0000-0002-2338-4364", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4c369b6006c44f47a2f8801632b05c55.json"}}, {"family": "Helmy", "given": "Adel", "initials": "A"}], "type": "journal article", "published": "2025-11-00", "journal": {"title": "J. Neurotrauma", "issn": "1557-9042", "volume": "42", "issue": "21-22", "pages": "1974-1984", "issn-l": "0897-7151"}, "abstract": "Disturbed cerebral autoregulation (represented by a positive pressure reactivity index [PRx]), elevated intracranial pressure (ICP), and decreased cerebral perfusion pressure (CPP) are key treatment targets following severe traumatic brain injury (sTBI). This study investigated neuroinflammation as a potential mechanism underlying these intracranial disturbances. Plasma samples from 11 sTBI patients (from a prior Phase II drug trial) were analyzed for 174 proteins using an antibody-based suspension bead array, with intervention effects accounted for where possible. Dimensionality reduction techniques, including principal component analysis (PCA) and supervised methods, were applied to protein data, informed by physiological variables (ICP, CPP, and PRx). PCA revealed distinct protein clustering patterns related to ICP >20 mmHg and PRx > 0, with PC1 linked to patient ID, time from injury, and intervention, and PC2/PC3 significantly associated with PRx dose (p < 0.001). Markers relating to inflammation of the vascular system comprised 20% of the top 50 proteins influencing PC2, implicating complement inflammation in these processes. Notably, MASP-2 (p = 0.027) and complement factor I (p = 0.039) were significantly associated with PRx dose in a mixed-effects model. These findings suggest that vascular inflammation, particularly complement activation, may contribute to intracranial physiological disturbances in sTBI, highlighting the complement pathway as a potential target for further investigation.", "doi": "10.1177/08977151251363979", "pmid": "40763090", "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T12:17:09.342Z", "modified": "2026-08-20T12:17:09.537Z"}, {"entity": "publication", "iuid": "7bb7900f2ac741948fd34f4105d1494e", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/7bb7900f2ac741948fd34f4105d1494e.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/7bb7900f2ac741948fd34f4105d1494e"}}, "title": "Fluid proteomics of CSF and serum reveal important neuroinflammatory proteins in blood-brain barrier disruption and outcome prediction following severe traumatic brain injury: a prospective, observational study.", "authors": [{"family": "Lindblad", "given": "Caroline", "initials": "C", "orcid": "0000-0003-4952-8597", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b3fe10d5ae0a4812a87c1d416201d408.json"}}, {"family": "Pin", "given": "Elisa", "initials": "E"}, {"family": "Just", "given": "David", "initials": "D"}, {"family": "Al Nimer", "given": "Faiez", "initials": "F"}, {"family": "Nilsson", "given": "Peter", "initials": "P"}, {"family": "Bellander", "given": "Bo-Michael", "initials": "BM"}, {"family": "Svensson", "given": "Mikael", "initials": "M"}, {"family": "Piehl", "given": "Fredrik", "initials": "F"}, {"family": "Thelin", "given": "Eric Peter", "initials": "EP"}], "type": "journal article", "published": "2021-03-12", "journal": {"title": "Crit Care", "issn": "1466-609X", "volume": "25", "issue": "1", "pages": "103", "issn-l": null}, "abstract": "Severe traumatic brain injury (TBI) is associated with blood-brain barrier (BBB) disruption and a subsequent neuroinflammatory process. We aimed to perform a multiplex screening of brain enriched and inflammatory proteins in blood and cerebrospinal fluid (CSF) in order to study their role in BBB disruption, neuroinflammation and long-term functional outcome in TBI patients and healthy controls.\n\nWe conducted a prospective, observational study on 90 severe TBI patients and 15 control subjects. Clinical outcome data, Glasgow Outcome Score, was collected after 6-12 months. We utilized a suspension bead antibody array analyzed on a FlexMap 3D Luminex platform to characterize 177 unique proteins in matched CSF and serum samples. In addition, we assessed BBB disruption using the CSF-serum albumin quotient (QA), and performed Apolipoprotein E-genotyping as the latter has been linked to BBB function in the absence of trauma. We employed pathway-, cluster-, and proportional odds regression analyses. Key findings were validated in blood samples from an independent TBI cohort.\n\nTBI patients had an upregulation of structural CNS and neuroinflammatory pathways in both CSF and serum. In total, 114 proteins correlated with QA, among which the top-correlated proteins were complement proteins. A cluster analysis revealed protein levels to be strongly associated with BBB integrity, but not carriage of the Apolipoprotein E4-variant. Among cluster-derived proteins, innate immune pathways were upregulated. Forty unique proteins emanated as novel independent predictors of clinical outcome, that individually explained ~ 10% additional model variance. Among proteins significantly different between TBI patients with intact or disrupted BBB, complement C9 in CSF (p = 0.014, \u0394R2 = 7.4%) and complement factor B in serum (p = 0.003, \u0394R2 = 9.2%) were independent outcome predictors also following step-down modelling.\n\nThis represents the largest concomitant CSF and serum proteomic profiling study so far reported in TBI, providing substantial support to the notion that neuroinflammatory markers, including complement activation, predicts BBB disruption and long-term outcome. Individual proteins identified here could potentially serve to refine current biomarker modelling or represent novel treatment targets in severe TBI.", "doi": "10.1186/s13054-021-03503-x", "pmid": "33712077", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC7955664"}, {"db": "pii", "key": "10.1186/s13054-021-03503-x"}], "notes": [], "created": "2026-08-20T12:21:28.337Z", "modified": "2026-08-20T12:21:28.418Z"}, {"entity": "publication", "iuid": "31007adaf22a4a3fa3482358ab1be096", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/31007adaf22a4a3fa3482358ab1be096.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/31007adaf22a4a3fa3482358ab1be096"}}, "title": "Fluid Proteomics of CSF and Serum Reveal Important Neuroinflammatory Proteins in Blood-Brain Barrier Disruption and Outcome Prediction following Severe Traumatic Brain Injury: a Prospective, Observational Study", "authors": [{"family": "Lindblad", "given": "Caroline", "initials": "C", "orcid": "0000-0003-4952-8597", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b3fe10d5ae0a4812a87c1d416201d408.json"}}, {"family": "Pin", "given": "Elisa", "initials": "E"}, {"family": "Just", "given": "David", "initials": "D"}, {"family": "Nimer", "given": "Faiez Al", "initials": "FA"}, {"family": "Nilsson", "given": "Peter", "initials": "P"}, {"family": "Bellander", "given": "Bo Michael", "initials": "BM"}, {"family": "Svensson", "given": "Mikael", "initials": "M"}, {"family": "Piehl", "given": "Fredrik", "initials": "F"}, {"family": "Thelin", "given": "Eric Peter", "initials": "EP"}], "type": "posted-content", "published": "2021-01-27", "journal": {"issn-l": null}, "abstract": null, "doi": "10.21203/rs.3.rs-96625/v2", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T13:00:13.416Z", "modified": "2026-08-20T13:00:13.466Z"}, {"entity": "publication", "iuid": "c3f8567a38884ab48f23ee663c5c67ae", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/c3f8567a38884ab48f23ee663c5c67ae.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/c3f8567a38884ab48f23ee663c5c67ae"}}, "title": "Fluid Proteomics of CSF and Serum Reveal Important Neuroinflammatory Proteins in Blood-Brain Barrier Disruption and Outcome Prediction Following Severe Traumatic Brain Injury: A Prospective, Observational Study", "authors": [{"family": "Lindblad", "given": "Caroline", "initials": "C", "orcid": "0000-0003-4952-8597", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b3fe10d5ae0a4812a87c1d416201d408.json"}}, {"family": "Pin", "given": "Elisa", "initials": "E"}, {"family": "Just", "given": "David", "initials": "D"}, {"family": "Nimer", "given": "Faiez Al", "initials": "FA"}, {"family": "Nilsson", "given": "Peter", "initials": "P"}, {"family": "Bellander", "given": "Bo Michael", "initials": "BM"}, {"family": "Svensson", "given": "Mikael", "initials": "M"}, {"family": "Piehl", "given": "Fredrik", "initials": "F"}, {"family": "Thelin", "given": "Eric Peter", "initials": "EP"}], "type": "posted-content", "published": "2020-10-29", "journal": {"issn-l": null}, "abstract": null, "doi": "10.21203/rs.3.rs-96625/v1", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T13:00:11.422Z", "modified": "2026-08-20T13:00:11.465Z"}]}