{"entity": "researcher", "timestamp": "2026-08-20T20:41:18.247Z", "family": "Porras Hern\u00e1ndez", "given": "A M", "initials": "AM", "orcid": "0000-0001-5952-2418", "affiliations": ["Science for Life Laboratory, Department of Materials Science and Engineering, Uppsala University, Uppsala, Sweden."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/b3c7742b33f8499baad542f0a2738a27.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/b3c7742b33f8499baad542f0a2738a27"}}, "publications": [{"entity": "publication", "iuid": "7e4434caa19c49c0be320d4f43e61fa3", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/7e4434caa19c49c0be320d4f43e61fa3.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/7e4434caa19c49c0be320d4f43e61fa3"}}, "title": "Confocal imaging dataset to assess endothelial cell orientation during extreme glucose conditions.", "authors": [{"family": "Porras Hern\u00e1ndez", "given": "Ana Mar\u00eda", "initials": "AM", "orcid": "0000-0001-5952-2418", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b3c7742b33f8499baad542f0a2738a27.json"}}, {"family": "Barbe", "given": "Laurent", "initials": "L"}, {"family": "Pohlit", "given": "Hannah", "initials": "H", "orcid": "0000-0002-6366-7836", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/51f92fd9b801400d97400b78a937db6c.json"}}, {"family": "Tenje", "given": "Maria", "initials": "M", "orcid": "0000-0002-1264-1337", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/bd13273274574923b354ca9c605efc8f.json"}}, {"family": "Antfolk", "given": "Maria", "initials": "M", "orcid": "0000-0003-0129-5640", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6d2f93e8ea8f4960801202f708186f84.json"}}], "type": "dataset", "published": "2022-01-27", "journal": {"title": "Sci Data", "issn": "2052-4463", "volume": "9", "issue": "1", "pages": "26", "issn-l": "2052-4463"}, "abstract": "Confocal microscopy offers a mean to extract quantitative data on spatially confined subcellular structures. Here, we provide an imaging dataset of confocal z-stacks on endothelial cells spatially confined on lines with different widths, visualizing the nucleus, F-actin, and zonula occludens-1 (ZO-1), as well as the lines. This dataset also includes confocal images of spatially confined endothelial cells challenged with different glucose conditions. We have validated the image quality by established analytical means using the MeasureImageQuality module of the CellProfilerTM software. We envision that this dataset could be used to extract data on both a population and a single cell level, as well as a learning set for the development of new image analysis tools.", "doi": "10.1038/s41597-022-01130-x", "pmid": "35087120", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC8795398"}, {"db": "pii", "key": "10.1038/s41597-022-01130-x"}], "notes": [], "created": "2026-08-20T09:04:13.297Z", "modified": "2026-08-20T09:04:13.391Z"}, {"entity": "publication", "iuid": "7f0fc73121214aecafb2fc59b2247484", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/7f0fc73121214aecafb2fc59b2247484.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/7f0fc73121214aecafb2fc59b2247484"}}, "title": "A simplified approach to control cell adherence on biologically derived in vitro cell culture scaffolds by direct UV-mediated RGD linkage.", "authors": [{"family": "Porras Hern\u00e1ndez", "given": "A M", "initials": "AM", "orcid": "0000-0001-5952-2418", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b3c7742b33f8499baad542f0a2738a27.json"}}, {"family": "Pohlit", "given": "H", "initials": "H"}, {"family": "Sj\u00f6gren", "given": "F", "initials": "F"}, {"family": "Shi", "given": "L", "initials": "L"}, {"family": "Ossipov", "given": "D", "initials": "D"}, {"family": "Antfolk", "given": "M", "initials": "M"}, {"family": "Tenje", "given": "M", "initials": "M"}], "type": "journal article", "published": "2020-10-14", "journal": {"title": "J Mater Sci Mater Med", "issn": "1573-4838", "volume": "31", "issue": "10", "pages": "89", "issn-l": "0957-4530"}, "abstract": "In this work, we present a method to fabricate a hyaluronic acid (HA) hydrogel with spatially controlled cell-adhesion properties based on photo-polymerisation cross-linking and functionalization. The approach utilises the same reaction pathway for both steps meaning that it is user-friendly and allows for adaptation at any stage during the fabrication process. Moreover, the process does not require any additional cross-linkers. The hydrogel is formed by UV-initiated radical addition reaction between acrylamide (Am) groups on the HA backbone. Cell adhesion is modulated by functionalising the adhesion peptide sequence arginine-glycine-aspartate onto the hydrogel surface via radical mediated thiol-ene reaction using the non-reacted Am groups. We show that 10 \u00d7 10 \u00b5m2 squares could be patterned with sharp features and a good resolution. The smallest area that could be patterned resulting in good cell adhesion was 25 \u00d7 25 \u00b5m2 squares, showing single-cell adhesion. Mouse brain endothelial cells adhered and remained in culture for up to 7 days on 100 \u00d7 100 \u00b5m2 square patterns. We see potential for this material combination for future use in novel organ-on-chip models and tissue engineering where the location of the cells is of importance and to further study endothelial cell biology.", "doi": "10.1007/s10856-020-06446-x", "pmid": "33057798", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC7560931"}, {"db": "pii", "key": "10.1007/s10856-020-06446-x"}], "notes": [], "created": "2026-08-20T06:40:01.694Z", "modified": "2026-08-20T06:40:01.786Z"}]}