{"entity": "researcher", "timestamp": "2026-08-20T20:36:56.471Z", "family": "Syr\u00e9n", "given": "Per\u2010Olof", "initials": "P", "orcid": "0000-0002-4066-2776", "affiliations": ["School of Chemistry Biotechnology and Health Science for Life Laboratory KTH Royal Institute of Technology  Solna Sweden", "School of Engineering Sciences in Chemistry Biotechnology and Health Department of Fibre and Polymer Technology KTH Royal Institute of Technology  Stockholm Sweden"], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/b035f26bd71e42f9942f9a4943ed0d1a.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/b035f26bd71e42f9942f9a4943ed0d1a"}}, "publications": [{"entity": "publication", "iuid": "eea2bf37d77c482db636e732034a81b6", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/eea2bf37d77c482db636e732034a81b6.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/eea2bf37d77c482db636e732034a81b6"}}, "title": "Engineering of an Ancestral McbA with Enhanced Domain Mobility Extends Biocatalytic Amide Synthesis Scope", "authors": [{"family": "S\u00f6derberg", "given": "Elisabeth", "initials": "E"}, {"family": "Molenaar", "given": "Marianne R", "initials": "MR"}, {"family": "Zaczyk", "given": "Katarzyna", "initials": "K"}, {"family": "Johansson", "given": "Magnus", "initials": "M"}, {"family": "Hayes", "given": "Martin A", "initials": "MA"}, {"family": "Syr\u00e9n", "given": "Per\u2010Olof", "initials": "P", "orcid": "0000-0002-4066-2776", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b035f26bd71e42f9942f9a4943ed0d1a.json"}}], "type": "journal-article", "published": "2026-01-00", "journal": {"title": "Adv. Synth. Catal.", "issn": "1615-4150", "volume": "368", "issue": "2", "issn-l": null}, "abstract": null, "doi": "10.1002/adsc.70232", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T06:28:11.405Z", "modified": "2026-08-20T06:28:11.495Z"}, {"entity": "publication", "iuid": "c2d83a423ae742cfb6fac787734d6525", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/c2d83a423ae742cfb6fac787734d6525.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/c2d83a423ae742cfb6fac787734d6525"}}, "title": "Progress and future outlook towards a safe and sustainable production and use of chemicals.", "authors": [{"family": "Munthe", "given": "John", "initials": "J", "orcid": "0000-0001-6118-5561", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4f2e53786d4946a190cab75f4f8d48ac.json"}}, {"family": "Bergstr\u00f6m", "given": "Lennart", "initials": "L"}, {"family": "Bolinius", "given": "D\u00e4mien", "initials": "D"}, {"family": "Cotgreave", "given": "Ian", "initials": "I"}, {"family": "Hellstr\u00f6m", "given": "Anna-Karin", "initials": "AK"}, {"family": "Holmquist", "given": "Hanna", "initials": "H"}, {"family": "Johansson", "given": "Magnus J", "initials": "MJ", "orcid": "0000-0002-0904-2835", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1ee8ab1a1e3b4480b2d1db9d62e0ded0.json"}}, {"family": "Julander", "given": "Anneli", "initials": "A", "orcid": "0000-0003-2131-4896", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d5e53578912d48ed8005581325c4ce46.json"}}, {"family": "Lihammar", "given": "Richard", "initials": "R"}, {"family": "Martin", "given": "Jonathan", "initials": "J"}, {"family": "Mart\u00edn-Matute", "given": "Bel\u00e9n", "initials": "B", "orcid": "0000-0002-7898-317X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6cfa83f2d4934ca5ab35c1276207d874.json"}}, {"family": "Mathew", "given": "Aji P", "initials": "AP"}, {"family": "Rydberg", "given": "Tomas", "initials": "T"}, {"family": "Skedung", "given": "Lisa", "initials": "L", "orcid": "0000-0001-6657-1592", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/12997dc67f144cda8a200b01095ba2c2.json"}}, {"family": "Witala", "given": "Monika", "initials": "M", "orcid": "0009-0008-4174-2082", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/db5a442579284d46ba0a2d9a756b74e8.json"}}, {"family": "Syr\u00e9n", "given": "Per-Olof", "initials": "PO", "orcid": "0000-0002-4066-2776", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b035f26bd71e42f9942f9a4943ed0d1a.json"}}], "type": "journal article", "published": "2025-11-14", "journal": {"title": "Commun Chem", "issn": "2399-3669", "volume": "8", "issue": "1", "pages": "350", "issn-l": null}, "abstract": "The demands on chemical industry to transform towards safety and sustainability will require multi-disciplinary research and development where experts on chemistry and chemical engineering, toxicology, ecotoxicology, and life cycle assessment collaborate to develop novel production methods, chemicals and materials. Here we summarise the results of the Mistra SafeChem programme which has yielded considerable output in the areas of catalysis/bio-catalysis, hazard screening for humans and ecosystems and life cycle assessment with chemical footprints, both as individual scientific achievements and as part of an integrated approach to assess safety and sustainability of novel chemicals and chemical synthesis processes, in chemical value chains and across collaborations between industry- academia/ industry-industry. The outcomes from the programme are summarised and discussed and experiences from dialogues on the future of safe and sustainable chemistry are presented.", "doi": "10.1038/s42004-025-01785-8", "pmid": "41238739", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC12618680"}, {"db": "pii", "key": "10.1038/s42004-025-01785-8"}], "notes": [], "created": "2026-08-20T09:25:46.841Z", "modified": "2026-08-20T09:25:47.173Z"}, {"entity": "publication", "iuid": "b67ef61687144616a1fef1364c7daf2c", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/b67ef61687144616a1fef1364c7daf2c.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/b67ef61687144616a1fef1364c7daf2c"}}, "title": "Chemoenzymatic cascade depolymerization of plastics.", "authors": [{"family": "Sun", "given": "Shengwei", "initials": "S"}, {"family": "Syr\u00e9n", "given": "Per-Olof", "initials": "PO", "orcid": "0000-0002-4066-2776", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b035f26bd71e42f9942f9a4943ed0d1a.json"}}], "type": "journal article", "published": "2025-09-09", "journal": {"title": "Commun Chem", "issn": "2399-3669", "volume": "8", "issue": "1", "pages": "272", "issn-l": null}, "abstract": "Plastic waste management is challenged by the inefficiencies and environmental impact of traditional chemical recycling methods. Here, the authors explore the chemoenzymatic cascade depolymerization approach, which offers a promising and sustainable solution for transforming plastic waste into valuable products.", "doi": "10.1038/s42004-025-01679-9", "pmid": "40926055", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC12420817"}, {"db": "pii", "key": "10.1038/s42004-025-01679-9"}], "notes": [], "created": "2026-08-20T09:25:43.144Z", "modified": "2026-08-20T09:25:43.206Z"}, {"entity": "publication", "iuid": "15c41b274c4b41f0908aa1298dd5ac40", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/15c41b274c4b41f0908aa1298dd5ac40.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/15c41b274c4b41f0908aa1298dd5ac40"}}, "title": "Chemically Recyclable and Enzymatically Degradable Thermostable Polyesters with Inherent Strain from \u03b1-Pinene-Derived Chiral Diols", "authors": [{"family": "Ranjani", "given": "Ganapathy", "initials": "G", "orcid": "0000-0002-0191-4758", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/96cf27fbe1d342639e6a72fe3a6fb821.json"}}, {"family": "Subramaniyan", "given": "Sathiyaraj", "initials": "S", "orcid": "0000-0002-2477-6896", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ee3e646506284d49a0d58b68b09f8719.json"}}, {"family": "Lopez-Lorenzo", "given": "Ximena", "initials": "X"}, {"family": "Hakkarainen", "given": "Minna", "initials": "M", "orcid": "0000-0002-7790-8987", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9d855814ea154871b096568c3e1d66a6.json"}}, {"family": "Syr\u00e9n", "given": "Per Olof", "initials": "PO", "orcid": "0000-0002-4066-2776", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b035f26bd71e42f9942f9a4943ed0d1a.json"}}], "type": "journal-article", "published": "2025-05-12", "journal": {"title": "ACS Sustainable Chem. Eng.", "issn": "2168-0485", "volume": "13", "issue": "18", "pages": "6696-6705", "issn-l": null}, "abstract": null, "doi": "10.1021/acssuschemeng.5c01374", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T08:13:51.974Z", "modified": "2026-08-20T08:45:41.146Z"}, {"entity": "publication", "iuid": "246a3e090e074644a1b77180979293d8", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/246a3e090e074644a1b77180979293d8.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/246a3e090e074644a1b77180979293d8"}}, "title": "Conformational Selection in Enzyme-Catalyzed Depolymerization of Bio-based Polyesters.", "authors": [{"family": "Lopez-Lorenzo", "given": "Ximena", "initials": "X"}, {"family": "Ranjani", "given": "Ganapathy", "initials": "G"}, {"family": "Syr\u00e9n", "given": "Per-Olof", "initials": "PO", "orcid": "0000-0002-4066-2776", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b035f26bd71e42f9942f9a4943ed0d1a.json"}}], "type": "journal article", "published": "2025-01-14", "journal": {"title": "Chembiochem", "issn": "1439-7633", "volume": "26", "issue": "2", "pages": "e202400456", "issn-l": "1439-4227"}, "abstract": "Enzymatic degradation of polymers holds promise for advancing towards a bio-based economy. However, the bulky nature of polymers presents challenges in accessibility for biocatalysts, hindering depolymerization reactions. Beyond the impact of crystallinity, polymer chains can reside in different conformations affecting binding efficiency to the enzyme active site. We previously showed that the gauche and trans chain conformers associated with crystalline and amorphous regions of the synthetic polyethylene terephthalate (PET) display different affinity to PETase, thus affecting the depolymerization rate. However, structural-function relationships for biopolymers remain poorly understood in biocatalysis. In this study, we explored the biodegradation of previously synthesized bio-polyesters made from a rigid bicyclic chiral terpene-based diol and copolymerized with various renewable diesters. Herein, four of those polyesters spanning from semi-aromatic to aliphatic were subjected to enzymatic degradations in concert with induced-fit docking (IFD) analyses. The monomer yield following enzymatic depolymerization by IsPETase S238 A, Dura and LCC ranged from 2 % to 17 % without any further pre-treatment step. The degradation efficiency was found to correlate with the extent of matched substrate and enzyme conformations revealed by IFD, regardless of the actual reaction temperature employed. Our findings demonstrate the importance of conformational selection in enzymatic depolymerization of biopolymers. A straight or twisted conformation of the polymer chain is crucial in biocatalytic degradation by showing different affinities to enzyme ground-state conformers. This work highlights the importance of considering the conformational match between the polymer and the enzyme to optimize the biocatalytic degradation efficiency of biopolymers, providing valuable insights for the development of sustainable bioprocesses.", "doi": "10.1002/cbic.202400456", "pmid": "39036936", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC11776369"}], "notes": [], "created": "2026-08-20T06:30:55.261Z", "modified": "2026-08-20T06:30:55.282Z"}, {"entity": "publication", "iuid": "58e6a7676f064486972b599a23050595", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/58e6a7676f064486972b599a23050595.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/58e6a7676f064486972b599a23050595"}}, "title": "Front Cover: Conformational Selection in Enzyme\u2010Catalyzed Depolymerization of Bio\u2010based Polyesters (ChemBioChem 2/2025)", "authors": [{"family": "Lopez\u2010Lorenzo", "given": "Ximena", "initials": "X"}, {"family": "Ranjani", "given": "Ganapathy", "initials": "G"}, {"family": "Syr\u00e9n", "given": "Per\u2010Olof", "initials": "P", "orcid": "0000-0002-4066-2776", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b035f26bd71e42f9942f9a4943ed0d1a.json"}}], "type": "journal-article", "published": "2025-01-14", "journal": {"title": "Chembiochem", "issn": "1439-4227", "volume": "26", "issue": "2", "issn-l": null}, "abstract": null, "doi": "10.1002/cbic.202580201", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T06:30:57.527Z", "modified": "2026-08-20T06:30:57.551Z"}, {"entity": "publication", "iuid": "a4860a0099d04b25bc0f711027397904", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/a4860a0099d04b25bc0f711027397904.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/a4860a0099d04b25bc0f711027397904"}}, "title": "Degradation of PET microplastic particles to monomers in human serum by PETase.", "authors": [{"family": "Lopez-Lorenzo", "given": "Ximena", "initials": "X", "orcid": "0000-0002-4708-9861", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/49eec96b36fd4880b09564c0b7688932.json"}}, {"family": "Hueting", "given": "David", "initials": "D"}, {"family": "Bosshard", "given": "Eliott", "initials": "E"}, {"family": "Syr\u00e9n", "given": "Per-Olof", "initials": "PO", "orcid": "0000-0002-4066-2776", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b035f26bd71e42f9942f9a4943ed0d1a.json"}}], "type": "journal article", "published": "2024-09-11", "journal": {"title": "Faraday Discuss", "issn": "1364-5498", "volume": "252", "issue": "0", "pages": "387-402", "issn-l": null}, "abstract": "More than 8 billion tons of plastic waste has been generated, posing severe environmental consequences and health risks. Due to prolonged exposure, microplastic particles are found in human blood and other bodily fluids. Despite a lack of toxicity studies regarding microplastics, harmful effects for humans seem plausible and cannot be excluded. As small plastic particles readily translocate from the gut to body fluids, enzyme-based treatment of serum could constitute a promising future avenue to clear synthetic polymers and their corresponding oligomers via their degradation into monomers of lower toxicity than the material they originate from. Still, whereas it is known that the enzymatic depolymerization rate of synthetic polymers varies by orders of magnitude depending on the buffer and media composition, the activity of plastic-degrading enzymes in serum was unknown. Here, we report how an engineered PETase, which we show to be generally trans-selective via induced fit docking, can depolymerize two different microplastic-like substrates of the commodity polymer polyethylene terephthalate (PET) into its non-toxic monomer terephthalic acid (TPA) alongside mono(2-hydroxyethyl)terephthalate (MHET) in human serum at 37 \u00b0C. We show that the application of PETase does not influence cell viability in vitro. Our work highlights the potential of applying biocatalysis in biomedicine and represents a first step towards finding a future solution to the problem that microplastics in the bloodstream may pose.", "doi": "10.1039/d4fd00014e", "pmid": "38864456", "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T09:27:49.535Z", "modified": "2026-08-20T09:27:49.633Z"}, {"entity": "publication", "iuid": "1fb121142bc844809f57c4726a2fd03a", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/1fb121142bc844809f57c4726a2fd03a.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/1fb121142bc844809f57c4726a2fd03a"}}, "title": "Fast Depolymerization of PET Bottle Mediated by Microwave Pre-Treatment and An Engineered PETase.", "authors": [{"family": "Guo", "given": "Boyang", "initials": "B", "orcid": "0000-0001-5510-1392", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/be843859e5a34c66b2c914c6f2bb955f.json"}}, {"family": "Lopez-Lorenzo", "given": "Ximena", "initials": "X", "orcid": "0000-0002-4708-9861", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/49eec96b36fd4880b09564c0b7688932.json"}}, {"family": "Fang", "given": "Yuan", "initials": "Y"}, {"family": "B\u00e4ckstr\u00f6m", "given": "Eva", "initials": "E"}, {"family": "Capezza", "given": "Antonio Jose", "initials": "AJ"}, {"family": "Vanga", "given": "Sudarsan Reddy", "initials": "SR"}, {"family": "Fur\u00f3", "given": "Istv\u00e1n", "initials": "I"}, {"family": "Hakkarainen", "given": "Minna", "initials": "M", "orcid": "0000-0002-7790-8987", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9d855814ea154871b096568c3e1d66a6.json"}}, {"family": "Syr\u00e9n", "given": "Per-Olof", "initials": "PO", "orcid": "0000-0002-4066-2776", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b035f26bd71e42f9942f9a4943ed0d1a.json"}}], "type": "journal article", "published": "2023-09-22", "journal": {"title": "ChemSusChem", "issn": "1864-564X", "volume": "16", "issue": "18", "pages": "e202300742", "issn-l": null}, "abstract": "Recycling plastics is the key to reaching a sustainable materials economy. Biocatalytic degradation of plastics shows great promise by allowing selective depolymerization of man-made materials into constituent building blocks under mild aqueous conditions. However, insoluble plastics have polymer chains that can reside in different conformations and show compact secondary structures that offer low accessibility for initiating the depolymerization reaction by enzymes. In this work, we overcome these shortcomings by microwave irradiation as a pre-treatment process to deliver powders of polyethylene terephthalate (PET) particles suitable for subsequent biotechnology-assisted plastic degradation by previously generated engineered enzymes. An optimized microwave step resulted in 1400 times higher integral of released terephthalic acid (TPA) from high-performance liquid chromatography (HPLC), compared to original untreated PET bottle. Biocatalytic plastic hydrolysis of substrates originating from PET bottles responded to 78 % yield conversion from 2 h microwave pretreatment and 1 h enzymatic reaction at 30 \u00b0C. The increase in activity stems from enhanced substrate accessibility from the microwave step, followed by the administration of designer enzymes capable of accommodating oligomers and shorter chains released in a productive conformation.", "doi": "10.1002/cssc.202300742", "pmid": "37384425", "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T06:31:57.604Z", "modified": "2026-08-20T06:31:57.734Z"}, {"entity": "publication", "iuid": "56833404aab9402582ae4be726e561f8", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/56833404aab9402582ae4be726e561f8.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/56833404aab9402582ae4be726e561f8"}}, "title": "Fast Depolymerization of PET Bottle Mediated by Microwave Pre-Treatment and An Engineered PETase.", "authors": [{"family": "Guo", "given": "Boyang", "initials": "B"}, {"family": "Lopez-Lorenzo", "given": "Ximena", "initials": "X"}, {"family": "Fang", "given": "Yuan", "initials": "Y"}, {"family": "B\u00e4ckstr\u00f6m", "given": "Eva", "initials": "E"}, {"family": "Capezza", "given": "Antonio Jose", "initials": "AJ"}, {"family": "Vanga", "given": "Sudarsan Reddy", "initials": "SR"}, {"family": "Fur\u00f3", "given": "Istv\u00e1n", "initials": "I"}, {"family": "Hakkarainen", "given": "Minna", "initials": "M"}, {"family": "Syr\u00e9n", "given": "Per-Olof", "initials": "PO", "orcid": "0000-0002-4066-2776", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b035f26bd71e42f9942f9a4943ed0d1a.json"}}], "type": "journal article", "published": "2023-09-22", "journal": {"title": "ChemSusChem", "issn": "1864-564X", "volume": "16", "issue": "18", "pages": "e202301237", "issn-l": null}, "abstract": "Invited for this month's cover is the groups of Prof. Minna Hakkarainen, Prof. Istv\u00e1n Fur\u00f3 and Assoc. Prof. Per-Olof Syr\u00e9n at KTH Royal Institute of Technology. The image shows how microwave irradiation is an efficient pre-treatment method of polyethylene terephthalate (PET) for subsequent biocatalytic depolymerization. The Research Article itself is available at 10.1002/cssc.202300742.", "doi": "10.1002/cssc.202301237", "pmid": "37679097", "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T06:31:59.531Z", "modified": "2026-08-20T06:31:59.605Z"}, {"entity": "publication", "iuid": "c7f0be383e3244a38fe4fa8c44712ccd", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/c7f0be383e3244a38fe4fa8c44712ccd.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/c7f0be383e3244a38fe4fa8c44712ccd"}}, "title": "Front Cover: Fast Depolymerization of PET Bottle Mediated by Microwave Pre\u2010Treatment and An Engineered PETase (ChemSusChem 18/2023)", "authors": [{"family": "Guo", "given": "Boyang", "initials": "B", "orcid": "0000-0001-5510-1392", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/be843859e5a34c66b2c914c6f2bb955f.json"}}, {"family": "Lopez\u2010Lorenzo", "given": "Ximena", "initials": "X", "orcid": "0000-0002-4708-9861", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/49eec96b36fd4880b09564c0b7688932.json"}}, {"family": "Fang", "given": "Yuan", "initials": "Y"}, {"family": "B\u00e4ckstr\u00f6m", "given": "Eva", "initials": "E"}, {"family": "Capezza", "given": "Antonio Jose", "initials": "AJ"}, {"family": "Vanga", "given": "Sudarsan Reddy", "initials": "SR"}, {"family": "Fur\u00f3", "given": "Istv\u00e1n", "initials": "I"}, {"family": "Hakkarainen", "given": "Minna", "initials": "M", "orcid": "0000-0002-7790-8987", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9d855814ea154871b096568c3e1d66a6.json"}}, {"family": "Syr\u00e9n", "given": "Per\u2010Olof", "initials": "P", "orcid": "0000-0002-4066-2776", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b035f26bd71e42f9942f9a4943ed0d1a.json"}}], "type": "journal-article", "published": "2023-09-22", "journal": {"title": "ChemSusChem", "issn": "1864-564X", "volume": "16", "issue": "18", "issn-l": null}, "abstract": null, "doi": "10.1002/cssc.202301238", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T06:32:06.636Z", "modified": "2026-08-20T06:32:06.715Z"}, {"entity": "publication", "iuid": "c0053b745a2e4ba5a7bb93c765e37042", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/c0053b745a2e4ba5a7bb93c765e37042.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/c0053b745a2e4ba5a7bb93c765e37042"}}, "title": "A Career in Catalysis: Bernhard Hauer", "authors": [{"family": "Nebel", "given": "Bernd A", "initials": "BA"}, {"family": "Breuer", "given": "Michael", "initials": "M"}, {"family": "Schneider", "given": "Andreas", "initials": "A"}, {"family": "Aberle", "given": "Benjamin", "initials": "B", "orcid": "0000-0002-1649-0425", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c335564b6dc0418899984ee9148f09ad.json"}}, {"family": "Hammer", "given": "Stephan C", "initials": "SC", "orcid": "0000-0002-3620-9362", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/427f8ec7a31e48b687350b32c7019f16.json"}}, {"family": "Syr\u00e9n", "given": "Per Olof", "initials": "PO", "orcid": "0000-0002-4066-2776", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b035f26bd71e42f9942f9a4943ed0d1a.json"}}, {"family": "Weissenborn", "given": "Martin J", "initials": "MJ"}, {"family": "Nestl", "given": "Bettina M", "initials": "BM", "orcid": "0000-0003-1282-195X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/00ebd29f71be4ae3ab79a17bfb2cc91c.json"}}], "type": "journal-article", "published": "2023-07-07", "journal": {"title": "ACS Catal", "issn": "2155-5435", "volume": "13", "issue": "13", "pages": "8861-8889", "issn-l": "2155-5435"}, "abstract": null, "doi": "10.1021/acscatal.3c01929", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T08:09:03.605Z", "modified": "2026-08-20T08:09:03.798Z"}, {"entity": "publication", "iuid": "3da30a4e62cf4a5abf5e34759d377acc", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/3da30a4e62cf4a5abf5e34759d377acc.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/3da30a4e62cf4a5abf5e34759d377acc"}}, "title": "Whole\u2010cell Mediated Carboxylation of 2\u2010Furoic Acid Towards the Production of Renewable Platform Chemicals and Biomaterials", "authors": [{"family": "Lopez\u2010Lorenzo", "given": "Ximena", "initials": "X", "orcid": "0000-0002-4708-9861", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/49eec96b36fd4880b09564c0b7688932.json"}}, {"family": "Asem", "given": "Heba", "initials": "H"}, {"family": "Stamm", "given": "Arne", "initials": "A"}, {"family": "Subramaniyan", "given": "Sathiyaraj", "initials": "S"}, {"family": "Hakkarainen", "given": "Minna", "initials": "M", "orcid": "0000-0002-7790-8987", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9d855814ea154871b096568c3e1d66a6.json"}}, {"family": "Syr\u00e9n", "given": "Per\u2010Olof", "initials": "P", "orcid": "0000-0002-4066-2776", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b035f26bd71e42f9942f9a4943ed0d1a.json"}}], "type": "journal-article", "published": "2023-03-22", "journal": {"title": "ChemCatChem", "issn": "1867-3880", "volume": "15", "issue": "6", "issn-l": null}, "abstract": null, "doi": "10.1002/cctc.202201483", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T06:31:01.098Z", "modified": "2026-08-20T06:31:01.242Z"}, {"entity": "publication", "iuid": "70e45bb685d3420da66426080592cb6e", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/70e45bb685d3420da66426080592cb6e.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/70e45bb685d3420da66426080592cb6e"}}, "title": "Designed for Circularity: Chemically Recyclable and Enzymatically Degradable Biorenewable Schiff Base Polyester-Imines", "authors": [{"family": "Subramaniyan", "given": "Sathiyaraj", "initials": "S"}, {"family": "Najjarzadeh", "given": "Nasim", "initials": "N"}, {"family": "Vanga", "given": "Sudarsana Reddy", "initials": "SR"}, {"family": "Liguori", "given": "Anna", "initials": "A"}, {"family": "Syr\u00e9n", "given": "Per Olof", "initials": "PO", "orcid": "0000-0002-4066-2776", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b035f26bd71e42f9942f9a4943ed0d1a.json"}}, {"family": "Hakkarainen", "given": "Minna", "initials": "M", "orcid": "0000-0002-7790-8987", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9d855814ea154871b096568c3e1d66a6.json"}}], "type": "journal-article", "published": "2023-02-27", "journal": {"title": "ACS Sustainable Chem. Eng.", "issn": "2168-0485", "volume": "11", "issue": "8", "pages": "3451-3465", "issn-l": null}, "abstract": null, "doi": "10.1021/acssuschemeng.2c06935", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T08:13:50.034Z", "modified": "2026-08-20T08:45:39.129Z"}, {"entity": "publication", "iuid": "7a51f32453164ef1998ab4c2fd24ad6d", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/7a51f32453164ef1998ab4c2fd24ad6d.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/7a51f32453164ef1998ab4c2fd24ad6d"}}, "title": "Thermoadaptation in an Ancestral Diterpene Cyclase by Altered Loop Stability.", "authors": [{"family": "Hueting", "given": "David A", "initials": "DA"}, {"family": "Vanga", "given": "Sudarsana R", "initials": "SR"}, {"family": "Syr\u00e9n", "given": "Per-Olof", "initials": "PO", "orcid": "0000-0002-4066-2776", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b035f26bd71e42f9942f9a4943ed0d1a.json"}}], "type": "journal article", "published": "2022-06-02", "journal": {"title": "J Phys Chem B", "issn": "1520-5207", "volume": "126", "issue": "21", "pages": "3809-3821", "issn-l": "1520-5207"}, "abstract": "Thermostability is the key to maintain the structural integrity and catalytic activity of enzymes in industrial biotechnological processes, such as terpene cyclase-mediated generation of medicines, chiral synthons, and fine chemicals. However, affording a large increase in the thermostability of enzymes through site-directed protein engineering techniques can constitute a challenge. In this paper, we used ancestral sequence reconstruction to create a hyperstable variant of the ent-copalyl diphosphate synthase PtmT2, a terpene cyclase involved in the assembly of antibiotics. Molecular dynamics simulations on the \u03bcs timescale were performed to shed light on possible molecular mechanisms contributing to activity at an elevated temperature and the large 40 \u00b0C increase in melting temperature observed for an ancestral variant of PtmT2. In silico analysis revealed key differences in the flexibility of a loop capping the active site, between extant and ancestral proteins. For the modern enzyme, the loop collapses into the active site at elevated temperatures, thus preventing biocatalysis, whereas the loop remains in a productive conformation both at ambient and high temperatures in the ancestral variant. Restoring a Pro loop residue introduced in the ancestral variant to the corresponding Gly observed in the extant protein led to reduced catalytic activity at high temperatures, with only moderate effects on the melting temperature, supporting the importance of the flexibility of the capping loop in thermoadaptation. Conversely, the inverse Gly to Pro loop mutation in the modern enzyme resulted in a 3-fold increase in the catalytic rate. Despite an overall decrease in maximal activity of ancestor compared to wild type, its increased thermostability provides a robust backbone amenable for further enzyme engineering. Our work cements the importance of loops in enzyme catalysis and provides a molecular mechanism contributing to thermoadaptation in an ancestral enzyme.", "doi": "10.1021/acs.jpcb.1c10605", "pmid": "35583961", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC9169049"}], "notes": [], "created": "2026-08-20T08:11:00.894Z", "modified": "2026-08-20T08:11:00.966Z"}, {"entity": "publication", "iuid": "d6315b5e655a4a468d500d6692c69532", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/d6315b5e655a4a468d500d6692c69532.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/d6315b5e655a4a468d500d6692c69532"}}, "title": "Conformational Selection in Biocatalytic Plastic Degradation by PETase", "authors": [{"family": "Guo", "given": "Boyang", "initials": "B", "orcid": "0000-0001-5510-1392", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/be843859e5a34c66b2c914c6f2bb955f.json"}}, {"family": "Vanga", "given": "Sudarsana Reddy", "initials": "SR"}, {"family": "Lopez-Lorenzo", "given": "Ximena", "initials": "X"}, {"family": "Saenz-Mendez", "given": "Patricia", "initials": "P", "orcid": "0000-0002-6711-4972", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0efb9bc5eb7344bea1f44272fa4b2540.json"}}, {"family": "Ericsson", "given": "Sara R\u00f6nnblad", "initials": "SR"}, {"family": "Fang", "given": "Yuan", "initials": "Y"}, {"family": "Ye", "given": "Xinchen", "initials": "X"}, {"family": "Schriever", "given": "Karen", "initials": "K", "orcid": "0000-0002-3677-5508", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/282cce0f6c054fd8a7858977be549b24.json"}}, {"family": "B\u00e4ckstr\u00f6m", "given": "Eva", "initials": "E"}, {"family": "Biundo", "given": "Antonino", "initials": "A"}, {"family": "Zubarev", "given": "Roman A", "initials": "RA", "orcid": "0000-0001-9839-2089", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5e3f9910c1ff434c8056bdecf537e9ef.json"}}, {"family": "Fur\u00f3", "given": "Istv\u00e1n", "initials": "I", "orcid": "0000-0002-0231-3970", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8e47a4c36b86429e8b17845c02387f5d.json"}}, {"family": "Hakkarainen", "given": "Minna", "initials": "M", "orcid": "0000-0002-7790-8987", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9d855814ea154871b096568c3e1d66a6.json"}}, {"family": "Syr\u00e9n", "given": "Per Olof", "initials": "PO", "orcid": "0000-0002-4066-2776", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b035f26bd71e42f9942f9a4943ed0d1a.json"}}], "type": "journal-article", "published": "2022-03-18", "journal": {"title": "ACS Catal", "issn": "2155-5435", "volume": "12", "issue": "6", "pages": "3397-3409", "issn-l": "2155-5435"}, "abstract": null, "doi": "10.1021/acscatal.1c05548", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T08:09:01.454Z", "modified": "2026-08-20T08:09:01.681Z"}, {"entity": "publication", "iuid": "0be796669e584dfa8ea662af78c39431", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/0be796669e584dfa8ea662af78c39431.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/0be796669e584dfa8ea662af78c39431"}}, "title": "Metabolite interactions in the bacterial Calvin cycle and implications for flux regulation", "authors": [{"family": "Sporre", "given": "Emil", "initials": "E", "orcid": "0000-0003-4105-7567", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/2250c46520424ec69e3d736c2bf30099.json"}}, {"family": "Karlsen", "given": "Jan", "initials": "J", "orcid": "0000-0002-2821-9026", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4a1c2fa8404749b0a797e90e9c6cb09e.json"}}, {"family": "Schriever", "given": "Karen", "initials": "K", "orcid": "0000-0002-3677-5508", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/282cce0f6c054fd8a7858977be549b24.json"}}, {"family": "Samuelsson", "given": "Johannes Asplund", "initials": "JA", "orcid": "0000-0001-8077-5305", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e1cf0c49cbb849b194b0b46582441d09.json"}}, {"family": "Janasch", "given": "Markus", "initials": "M", "orcid": "0000-0001-7745-720X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/115ab90674ba4090be1b3ef9a01549e4.json"}}, {"family": "Strandberg", "given": "Linn\u00e9a", "initials": "L", "orcid": "0000-0001-5386-5460", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5ea4741b77234afe83e92c3dfff0a799.json"}}, {"family": "Kotol", "given": "David", "initials": "D", "orcid": "0000-0002-5388-3826", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c41f8f491c474e6bba2047b5d04b4af5.json"}}, {"family": "Zeckey", "given": "Luise", "initials": "L"}, {"family": "Piazza", "given": "Ilaria", "initials": "I", "orcid": "0000-0001-5895-6134", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5258dce396444d9a8f5e5bc2fd13b282.json"}}, {"family": "Syr\u00e9n", "given": "Per Olof", "initials": "PO", "orcid": "0000-0002-4066-2776", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b035f26bd71e42f9942f9a4943ed0d1a.json"}}, {"family": "Edfors", "given": "Fredrik", "initials": "F", "orcid": "0000-0002-0017-7987", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9e213b04f8984c8eb00c68f5bc2f6a36.json"}}, {"family": "Hudson", "given": "Elton P", "initials": "EP", "orcid": "0000-0003-1899-7649", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3b1c894d4c8b48569b9879d0342b91dd.json"}}], "type": "posted-content", "published": "2022-03-15", "journal": {"issn-l": null}, "abstract": null, "doi": "10.1101/2022.03.15.483797", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T10:10:14.448Z", "modified": "2026-08-20T10:10:14.574Z"}, {"entity": "publication", "iuid": "679d54c06fb2446295f544963d738a89", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/679d54c06fb2446295f544963d738a89.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/679d54c06fb2446295f544963d738a89"}}, "title": "Pinene-Based Oxidative Synthetic Toolbox for Scalable Polyester Synthesis.", "authors": [{"family": "Stamm", "given": "Arne", "initials": "A"}, {"family": "\u00d6hlin", "given": "Johannes", "initials": "J"}, {"family": "Mosbech", "given": "Caroline", "initials": "C"}, {"family": "Ols\u00e9n", "given": "Peter", "initials": "P", "orcid": "0000-0002-5081-1835", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0ce2420fc18146fd810279237057d4fa.json"}}, {"family": "Guo", "given": "Boyang", "initials": "B"}, {"family": "S\u00f6derberg", "given": "Elisabeth", "initials": "E"}, {"family": "Biundo", "given": "Antonino", "initials": "A"}, {"family": "Fogelstr\u00f6m", "given": "Linda", "initials": "L"}, {"family": "Bhattacharyya", "given": "Shubhankar", "initials": "S", "orcid": "0000-0002-6295-4112", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5e7ea9677a5a4b4993a6196b4f9f4001.json"}}, {"family": "Bornscheuer", "given": "Uwe T", "initials": "UT", "orcid": "0000-0003-0685-2696", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0464afedde7643f19b28b66fb3497017.json"}}, {"family": "Malmstr\u00f6m", "given": "Eva", "initials": "E", "orcid": "0000-0002-8348-2273", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/783ef2434e294c068fe5202ed58417b1.json"}}, {"family": "Syr\u00e9n", "given": "Per-Olof", "initials": "PO", "orcid": "0000-0002-4066-2776", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b035f26bd71e42f9942f9a4943ed0d1a.json"}}], "type": "journal article", "published": "2021-11-22", "journal": {"title": "JACS Au", "issn": "2691-3704", "volume": "1", "issue": "11", "pages": "1949-1960", "issn-l": null}, "abstract": "Generation of renewable polymers is a long-standing goal toward reaching a more sustainable society, but building blocks in biomass can be incompatible with desired polymerization type, hampering the full implementation potential of biomaterials. Herein, we show how conceptually simple oxidative transformations can be used to unlock the inherent reactivity of terpene synthons in generating polyesters by two different mechanisms starting from the same \u03b1-pinene substrate. In the first pathway, \u03b1-pinene was oxidized into the bicyclic verbanone-based lactone and subsequently polymerized into star-shaped polymers via ring-opening polymerization, resulting in a biobased semicrystalline polyester with tunable glass transition and melting temperatures. In a second pathway, polyesters were synthesized via polycondensation, utilizing the diol 1-(1'-hydroxyethyl)-3-(2'-hydroxy-ethyl)-2,2-dimethylcyclobutane (HHDC) synthesized by oxidative cleavage of the double bond of \u03b1-pinene, together with unsaturated biobased diesters such as dimethyl maleate (DMM) and dimethyl itaconate (DMI). The resulting families of terpene-based polyesters were thereafter successfully cross-linked by either transetherification, utilizing the terminal hydroxyl groups of the synthesized verbanone-based materials, or by UV irradiation, utilizing the unsaturation provided by the DMM or DMI moieties within the HHDC-based copolymers. This work highlights the potential to apply an oxidative toolbox to valorize inert terpene metabolites enabling generation of biosourced polyesters and coatings thereof by complementary mechanisms.", "doi": "10.1021/jacsau.1c00312", "pmid": "34849510", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC8620555"}], "notes": [], "created": "2026-08-20T08:45:19.017Z", "modified": "2026-08-20T08:45:19.176Z"}, {"entity": "publication", "iuid": "b9149930663e43008ad85667ff1f72d9", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/b9149930663e43008ad85667ff1f72d9.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/b9149930663e43008ad85667ff1f72d9"}}, "title": "Biocatalysis in the Recycling Landscape for Synthetic Polymers and Plastics towards Circular Textiles.", "authors": [{"family": "J\u00f6nsson", "given": "Christina", "initials": "C", "orcid": "0000-0003-3124-1723", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/00a9f7e064a7479aa2dea0e0363b3077.json"}}, {"family": "Wei", "given": "Ren", "initials": "R"}, {"family": "Biundo", "given": "Antonino", "initials": "A"}, {"family": "Landberg", "given": "Johan", "initials": "J"}, {"family": "Schwarz Bour", "given": "Lisa", "initials": "L"}, {"family": "Pezzotti", "given": "Fabio", "initials": "F"}, {"family": "Toca", "given": "Andreea", "initials": "A"}, {"family": "M Jacques", "given": "Les", "initials": "L"}, {"family": "Bornscheuer", "given": "Uwe T", "initials": "UT", "orcid": "0000-0003-0685-2696", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0464afedde7643f19b28b66fb3497017.json"}}, {"family": "Syr\u00e9n", "given": "Per-Olof", "initials": "PO", "orcid": "0000-0002-4066-2776", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b035f26bd71e42f9942f9a4943ed0d1a.json"}}], "type": "journal article", "published": "2021-10-05", "journal": {"title": "ChemSusChem", "issn": "1864-564X", "volume": "14", "issue": "19", "pages": "4028-4040", "issn-l": null}, "abstract": "Although recovery of fibers from used textiles with retained material quality is desired, separation of individual components from polymer blends used in today's complex textile materials is currently not available at viable scale. Biotechnology could provide a solution to this pressing problem by enabling selective depolymerization of recyclable fibers of natural and synthetic origin, to isolate constituents or even recover monomers. We compiled experimental data for biocatalytic polymer degradation with a focus on synthetic polymers with hydrolysable links and calculated conversion rates to explore this path The analysis emphasizes that we urgently need major research efforts: beyond cellulose-based fibers, biotechnological-assisted depolymerization of plastics so far only works for polyethylene terephthalate, with degradation of a few other relevant synthetic polymer chains being reported. In contrast, by analyzing market data and emerging trends for synthetic fibers in the textile industry, in combination with numbers from used garment collection and sorting plants, it was shown that the use of difficult-to-recycle blended materials is rapidly growing. If the lack of recycling technology and production trend for fiber blends remains, a volume of more than 3400 Mt of waste will have been accumulated by 2030. This work highlights the urgent need to transform the textile industry from a biocatalytic perspective.", "doi": "10.1002/cssc.202002666", "pmid": "33497036", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC8518944"}], "notes": [], "created": "2026-08-20T06:31:55.037Z", "modified": "2026-08-20T06:31:55.204Z"}, {"entity": "publication", "iuid": "655947c203b44a9f8a690e2cf9961d78", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/655947c203b44a9f8a690e2cf9961d78.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/655947c203b44a9f8a690e2cf9961d78"}}, "title": "Engineering of Ancestors as a Tool to Elucidate Structure, Mechanism, and Specificity of Extant Terpene Cyclase.", "authors": [{"family": "Schriever", "given": "Karen", "initials": "K", "orcid": "0000-0002-3677-5508", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/282cce0f6c054fd8a7858977be549b24.json"}}, {"family": "Saenz-Mendez", "given": "Patricia", "initials": "P", "orcid": "0000-0002-6711-4972", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0efb9bc5eb7344bea1f44272fa4b2540.json"}}, {"family": "Rudraraju", "given": "Reshma Srilakshmi", "initials": "RS"}, {"family": "Hendrikse", "given": "Natalie M", "initials": "NM", "orcid": "0000-0001-8644-3408", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/711d4343f4594d87852869666b347f98.json"}}, {"family": "Hudson", "given": "Elton P", "initials": "EP"}, {"family": "Biundo", "given": "Antonino", "initials": "A"}, {"family": "Schnell", "given": "Robert", "initials": "R"}, {"family": "Syr\u00e9n", "given": "Per-Olof", "initials": "PO", "orcid": "0000-0002-4066-2776", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b035f26bd71e42f9942f9a4943ed0d1a.json"}}], "type": "journal article", "published": "2021-03-17", "journal": {"title": "Journal of the American Chemical Society", "issn": "1520-5126", "volume": "143", "issue": "10", "pages": "3794-3807", "issn-l": "0002-7863"}, "abstract": "Structural information is crucial for understanding catalytic mechanisms and to guide enzyme engineering efforts of biocatalysts, such as terpene cyclases. However, low sequence similarity can impede homology modeling, and inherent protein instability presents challenges for structural studies. We hypothesized that X-ray crystallography of engineered thermostable ancestral enzymes can enable access to reliable homology models of extant biocatalysts. We have applied this concept in concert with molecular modeling and enzymatic assays to understand the structure activity relationship of spiroviolene synthase, a class I terpene cyclase, aiming to engineer its specificity. Engineering a surface patch in the reconstructed ancestor afforded a template structure for generation of a high-confidence homology model of the extant enzyme. On the basis of structural considerations, we designed and crystallized ancestral variants with single residue exchanges that exhibited tailored substrate specificity and preserved thermostability. We show how the two single amino acid alterations identified in the ancestral scaffold can be transferred to the extant enzyme, conferring a specificity switch that impacts the extant enzyme's specificity for formation of the diterpene spiroviolene over formation of sesquiterpenes hedycaryol and farnesol by up to 25-fold. This study emphasizes the value of ancestral sequence reconstruction combined with enzyme engineering as a versatile tool in chemical biology.", "doi": "10.1021/jacs.0c10214", "pmid": "33496585", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC8023661"}], "notes": [], "created": "2026-08-20T08:45:04.766Z", "modified": "2026-08-20T08:45:04.843Z"}, {"entity": "publication", "iuid": "c1bc4891033b4abd952e740fa2122274", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/c1bc4891033b4abd952e740fa2122274.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/c1bc4891033b4abd952e740fa2122274"}}, "title": "Lactone monomers obtained by enzyme catalysis and their use in reversible thermoresponsive networks", "authors": [{"family": "Farhat", "given": "Wissam", "initials": "W", "orcid": "0000-0002-1050-3723", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e7fffd00742c488e99cf0891c5f9281d.json"}}, {"family": "Biundo", "given": "Antonino", "initials": "A"}, {"family": "Stamm", "given": "Arne", "initials": "A"}, {"family": "Malmstr\u00f6m", "given": "Eva", "initials": "E"}, {"family": "Syr\u00e9n", "given": "Per\u2010Olof", "initials": "P", "orcid": "0000-0002-4066-2776", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b035f26bd71e42f9942f9a4943ed0d1a.json"}}], "type": "journal-article", "published": "2020-05-10", "journal": {"title": "J of Applied Polymer Sci", "issn": "0021-8995", "volume": "137", "issue": "18", "issn-l": null}, "abstract": null, "doi": "10.1002/app.48949", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T06:30:03.949Z", "modified": "2026-08-20T06:30:04.054Z"}, {"entity": "publication", "iuid": "04e0f0670b5445558587c0b874596c18", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/04e0f0670b5445558587c0b874596c18.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/04e0f0670b5445558587c0b874596c18"}}, "title": "Switched reaction specificity in polyesterases towards amide bond hydrolysis by enzyme engineering.", "authors": [{"family": "Biundo", "given": "Antonino", "initials": "A"}, {"family": "Subagia", "given": "Raditya", "initials": "R"}, {"family": "Maurer", "given": "Michael", "initials": "M"}, {"family": "Ribitsch", "given": "Doris", "initials": "D", "orcid": "0000-0002-5822-0204", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/41d62192a04240488686a7039656222a.json"}}, {"family": "Syr\u00e9n", "given": "Per-Olof", "initials": "PO", "orcid": "0000-0002-4066-2776", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b035f26bd71e42f9942f9a4943ed0d1a.json"}}, {"family": "Guebitz", "given": "Georg M", "initials": "GM"}], "type": "journal article", "published": "2019-11-04", "journal": {"title": "RSC Adv.", "issn": "2046-2069", "volume": "9", "issue": "62", "pages": "36217-36226", "issn-l": "2046-2069"}, "abstract": "The recalcitrance of plastics like nylon and other polyamides contributes to environmental problems (e.g. microplastics in oceans) and restricts possibilities for recycling. The fact that hitherto discovered amidases (EC 3.5.1. and 3.5.2.) only show no, or low, activity on polyamides currently obstructs biotechnological-assisted depolymerization of man-made materials. In this work, we capitalized on enzyme engineering to enhance the promiscuous amidase activity of polyesterases. Through enzyme design we created a reallocated water network adapted for hydrogen bond formation to synthetic amide backbones for enhanced transition state stabilization in the polyester-hydrolyzing biocatalysts Humicola insolens cutinase and Thermobifida cellulosilytica cutinase 1. This novel concept enabled increased catalytic efficiency towards amide-containing soluble substrates. The afforded enhanced hydrolysis of the amide bond-containing insoluble substrate 3PA 6,6 by designed variants was aligned with improved transition state stabilization identified by molecular dynamics (MD) simulations. Furthermore, the presence of a favorable water-molecule network that interacted with synthetic amides in the variants resulted in a reduced activity on polyethylene terephthalate (PET). Our data demonstrate the potential of using enzyme engineering to improve the amidase activity for polyesterases to act on synthetic amide-containing polymers.", "doi": "10.1039/c9ra07519d", "pmid": "35540575", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC9074940"}, {"db": "pii", "key": "c9ra07519d"}], "notes": [], "created": "2026-08-20T09:27:15.703Z", "modified": "2026-08-20T09:27:15.818Z"}, {"entity": "publication", "iuid": "617edd69409e4be8b0615205dac1ab64", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/617edd69409e4be8b0615205dac1ab64.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/617edd69409e4be8b0615205dac1ab64"}}, "title": "A Retro-biosynthesis-Based Route to Generate Pinene-Derived Polyesters.", "authors": [{"family": "Stamm", "given": "Arne", "initials": "A"}, {"family": "Biundo", "given": "Antonino", "initials": "A"}, {"family": "Schmidt", "given": "Bj\u00f6rn", "initials": "B"}, {"family": "Br\u00fccher", "given": "J\u00f6rg", "initials": "J"}, {"family": "Lundmark", "given": "Stefan", "initials": "S"}, {"family": "Ols\u00e9n", "given": "Peter", "initials": "P"}, {"family": "Fogelstr\u00f6m", "given": "Linda", "initials": "L"}, {"family": "Malmstr\u00f6m", "given": "Eva", "initials": "E"}, {"family": "Bornscheuer", "given": "Uwe T", "initials": "UT"}, {"family": "Syr\u00e9n", "given": "Per-Olof", "initials": "PO", "orcid": "0000-0002-4066-2776", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b035f26bd71e42f9942f9a4943ed0d1a.json"}}], "type": "journal article", "published": "2019-07-01", "journal": {"title": "Chembiochem", "issn": "1439-7633", "volume": "20", "issue": "13", "pages": "1664-1671", "issn-l": "1439-4227"}, "abstract": "Significantly increased production of biobased polymers is a prerequisite to replace petroleum-based materials towards reaching a circular bioeconomy. However, many renewable building blocks from wood and other plant material are not directly amenable for polymerization, due to their inert backbones and/or lack of functional group compatibility with the desired polymerization type. Based on a retro-biosynthetic analysis of polyesters, a chemoenzymatic route from (-)-\u03b1-pinene towards a verbanone-based lactone, which is further used in ring-opening polymerization, is presented. Generated pinene-derived polyesters showed elevated degradation and glass transition temperatures, compared with poly(\u03f5-decalactone), which lacks a ring structure in its backbone. Semirational enzyme engineering of the cyclohexanone monooxygenase from Acinetobacter calcoaceticus enabled the biosynthesis of the key lactone intermediate for the targeted polyester. As a proof of principle, one enzyme variant identified from screening in a microtiter plate was used in biocatalytic upscaling, which afforded the bicyclic lactone in 39 % conversion in shake flask scale reactions.", "doi": "10.1002/cbic.201900046", "pmid": "30793830", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC6618282"}], "notes": [], "created": "2026-08-20T06:30:49.146Z", "modified": "2026-08-20T06:30:49.216Z"}, {"entity": "publication", "iuid": "33c34a9cedc94545be7f4a4fe82eebeb", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/33c34a9cedc94545be7f4a4fe82eebeb.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/33c34a9cedc94545be7f4a4fe82eebeb"}}, "title": "Biocatalysis for terpene-based polymers.", "authors": [{"family": "Farhat", "given": "Wissam", "initials": "W"}, {"family": "Stamm", "given": "Arne", "initials": "A"}, {"family": "Robert-Monpate", "given": "Maxime", "initials": "M"}, {"family": "Biundo", "given": "Antonino", "initials": "A"}, {"family": "Syr\u00e9n", "given": "Per-Olof", "initials": "PO", "orcid": "0000-0002-4066-2776", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b035f26bd71e42f9942f9a4943ed0d1a.json"}}], "type": "letter", "published": "2019-02-25", "journal": {"title": "Z Naturforsch C J Biosci", "issn": "1865-7125", "volume": "74", "issue": "3-4", "pages": "91-100", "issn-l": null}, "abstract": "Accelerated generation of bio-based materials is vital to replace current synthetic polymers obtained from petroleum with more sustainable options. However, many building blocks available from renewable resources mainly contain unreactive carbon-carbon bonds, which obstructs their efficient polymerization. Herein, we highlight the potential of applying biocatalysis to afford tailored functionalization of the inert carbocyclic core of multicyclic terpenes toward advanced materials. As a showcase, we unlock the inherent monomer reactivity of norcamphor, a bicyclic ketone used as a monoterpene model system in this study, to afford polyesters with unprecedented backbones. The efficiencies of the chemical and enzymatic Baeyer-Villiger transformation in generating key lactone intermediates are compared. The concepts discussed herein are widely applicable for the valorization of terpenes and other cyclic building blocks using chemoenzymatic strategies.", "doi": "10.1515/znc-2018-0199", "pmid": "30789828", "labels": [], "xrefs": [{"db": "pii", "key": "znc-2018-0199"}], "notes": [], "created": "2026-08-20T12:51:21.368Z", "modified": "2026-08-20T12:51:21.398Z"}, {"entity": "publication", "iuid": "e7fae8bdde6447de8ea3df1b68e0dd1e", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/e7fae8bdde6447de8ea3df1b68e0dd1e.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/e7fae8bdde6447de8ea3df1b68e0dd1e"}}, "title": "Enzymatic Hydrolysis of Tertiary Amide Bonds by anti Nucleophilic Attack and Protonation.", "authors": [{"family": "Syr\u00e9n", "given": "Per-Olof", "initials": "PO", "orcid": "0000-0002-4066-2776", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b035f26bd71e42f9942f9a4943ed0d1a.json"}}], "type": "journal article", "published": "2018-11-02", "journal": {"title": "J. Org. Chem.", "issn": "1520-6904", "volume": "83", "issue": "21", "pages": "13543-13548", "issn-l": "0022-3263"}, "abstract": "The molecular mechanisms conferring high resistance of planar tertiary amide bonds to hydrolysis by most enzymes have remained elusive. To provide a chemical explanation to this unresolved puzzle, UB3LYP calculations were performed on an active site model of Xaa-Pro peptidases. The calculated reaction mechanism demonstrates that biocatalysts capable of tertiary amide bond hydrolysis capitalize on anti nucleophilic attack and protonation of the amide nitrogen, in contrast to the traditional syn displayed by amidases and proteases acting on secondary amide bonds.", "doi": "10.1021/acs.joc.8b02053", "pmid": "30256635", "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T08:10:56.568Z", "modified": "2026-08-20T08:10:56.634Z"}, {"entity": "publication", "iuid": "e32e073882134847818207396f00ed79", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/e32e073882134847818207396f00ed79.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/e32e073882134847818207396f00ed79"}}, "title": "Cover Feature: Protonation\u2010Initiated Cyclization by a Class II Terpene Cyclase Assisted by Tunneling (ChemBioChem 23/2017)", "authors": [{"family": "Eriksson", "given": "Adam", "initials": "A", "orcid": "0000-0003-4780-6274", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6c2423747e9641e38ab2ce6c3f45d485.json"}}, {"family": "K\u00fcrten", "given": "Charlotte", "initials": "C", "orcid": "0000-0002-1685-4735", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/df5a1963de7a4135a3a4f2eda8a086bf.json"}}, {"family": "Syr\u00e9n", "given": "Per\u2010Olof", "initials": "P", "orcid": "0000-0002-4066-2776", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b035f26bd71e42f9942f9a4943ed0d1a.json"}}], "type": "journal-article", "published": "2017-12-05", "journal": {"title": "Chembiochem", "issn": "1439-4227", "volume": "18", "issue": "23", "pages": "2293-2293", "issn-l": null}, "abstract": null, "doi": "10.1002/cbic.201700602", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T06:30:46.896Z", "modified": "2026-08-20T06:30:47.055Z"}]}