{"entity": "researcher", "timestamp": "2026-08-22T06:57:26.229Z", "family": "Kokhaei", "given": "Parviz", "initials": "P", "orcid": "0000-0001-6544-4209", "affiliations": ["Cancer Research Center, Semnan University of Medical Sciences, Semnan, Iran.", "Department of Oncology-Pathology, BioClinicum, Karolinska University Hospital Solna and Karolinska Institutet, Stockholm, Sweden."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/adf579c1acba4c869fcf1866e7d5a83b.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/adf579c1acba4c869fcf1866e7d5a83b"}}, "publications": [{"entity": "publication", "iuid": "130d109be379434bbe8fbd9ee0684dfa", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/130d109be379434bbe8fbd9ee0684dfa.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/130d109be379434bbe8fbd9ee0684dfa"}}, "title": "Anti-tumor effect of berberine on chronic lymphocytic leukemia cells.", "authors": [{"family": "Abdollahi", "given": "Maryam", "initials": "M"}, {"family": "Mohammadlou", "given": "Maryam", "initials": "M"}, {"family": "Hemati", "given": "Maral", "initials": "M"}, {"family": "Baharlou", "given": "Rasoul", "initials": "R"}, {"family": "Manouchehri Doulabi", "given": "Ehsan", "initials": "E"}, {"family": "Ghahremanfard", "given": "Farahnaz", "initials": "F", "orcid": "0000-0003-2620-3378", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/7890039aef0d471ab8a4610890ba1fe8.json"}}, {"family": "Sarabi", "given": "Mohammad Amir", "initials": "MA"}, {"family": "Kokhaei", "given": "Parviz", "initials": "P", "orcid": "0000-0001-6544-4209", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/adf579c1acba4c869fcf1866e7d5a83b.json"}}], "type": "journal article", "published": "2022-09-29", "journal": {"title": "Med. Oncol.", "issn": "1559-131X", "volume": "39", "issue": "12", "pages": "217", "issn-l": "1357-0560"}, "abstract": "Chronic lymphocytic leukemia (CLL) is a blood malignancy that is characterized by remarkable expression of CD69 and Ki67 in CLL cells. Elevated levels of Cleaved-Poly (ADP-ribose) polymerase-1 (PARP1) and microRNA-155 (MiR-155) are related to poor prognosis of disease. Berberine as a natural isoquinoline alkaloid, has shown an anti-tumor potential in tumor cells. The objective of present study was to explore some aspects of molecular mechanisms of berberine effect in CLL cells. To analyze the expression of CD69 and Ki67 using flow cytometry, 16 peripheral blood samples and seven bone marrow aspirates were collected from CLL patients. Isolated peripheral blood mononuclear cells (PBMCs) and bone marrow mononuclear cells (BMMCs) were treated with 25 \u00b5M of berberine for 24 h. The level of miR-155 expression was subsequently evaluated by real-time PCR. Furthermore, western blot was used for assessment of cleaved PARP1. Our results demonstrated a significant reduction in CD69 and Ki67 expression on CD19+ cells when the cells were treated by berberine. Interestingly, the expression level of miR-155 was reduced after berberine treatment in compare to the control group. Furthermore, western blotting revealed an increased level of cleaved PARP1 in dose-dependently manner in CLL cells. The results confirmed the anti-tumor impact of berberine on CLL cells through reducing CD69, Ki67, and miR-155 expression and increasing cleaved PARP1 may be considered as an option for future clinical studies.", "doi": "10.1007/s12032-022-01818-5", "pmid": "36175700", "labels": [], "xrefs": [{"db": "pii", "key": "10.1007/s12032-022-01818-5"}], "notes": [], "created": "2026-08-21T11:08:50.575Z", "modified": "2026-08-21T11:08:50.627Z"}, {"entity": "publication", "iuid": "a064147d37fd4e9fbcd2e81d0574c85a", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/a064147d37fd4e9fbcd2e81d0574c85a.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/a064147d37fd4e9fbcd2e81d0574c85a"}}, "title": "Apoptotic effect of berberine via Bcl-2, ROR1, and mir-21 in patients with B-chronic lymphocytic leukemia.", "authors": [{"family": "Mohammadlou", "given": "Maryam", "initials": "M", "orcid": "0000-0001-5895-5035", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/df6a35c6efbe46a5869a2ef459c3d5cc.json"}}, {"family": "Abdollahi", "given": "Maryam", "initials": "M"}, {"family": "Hemati", "given": "Maral", "initials": "M", "orcid": "0000-0003-3869-598X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8255885cf463425c919d2dbd084073f2.json"}}, {"family": "Baharlou", "given": "Rasoul", "initials": "R", "orcid": "0000-0002-4785-2759", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6d703027df984ba19522a7b00c15ccfa.json"}}, {"family": "Doulabi", "given": "Ehsan Manouchehri", "initials": "EM", "orcid": "0000-0003-1639-2267", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ad12164c3f004575b93f26cf83df40eb.json"}}, {"family": "Pashaei", "given": "Mehrnoosh", "initials": "M"}, {"family": "Ghahremanfard", "given": "Farahnaz", "initials": "F", "orcid": "0000-0003-2620-3378", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/7890039aef0d471ab8a4610890ba1fe8.json"}}, {"family": "Faranoush", "given": "Mohammad", "initials": "M", "orcid": "0000-0002-2775-2347", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6a35ad59c5694182a5ea331a0d23b869.json"}}, {"family": "Kokhaei", "given": "Parviz", "initials": "P", "orcid": "0000-0001-6544-4209", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/adf579c1acba4c869fcf1866e7d5a83b.json"}}], "type": "journal article", "published": "2021-04-00", "journal": {"title": "Phytother Res", "issn": "1099-1573", "volume": "35", "issue": "4", "pages": "2025-2033", "issn-l": null}, "abstract": "Berberine is a natural isoquinoline alkaloid that has been shown to inhibit the proliferation and induce apoptosis in a wide variety of tumor cells. However, the action mechanism of berberine in CLL cells is unknown. The previous study has shown that berberine leads to reduced viability and elevated levels of apoptosis in PBMCs of CLL patients. CLL cells are characterized by remarkable expression of Bcl-2 and ROR1 which leads to activation and survival and increases disease progression in patients. High-level expression of miR-21 in patients with CLL is associated with a higher risk of death. Here we investigated the anticancer effects of berberine upon peripheral blood mononuclear cells (PBMCs) of CLL patients. To evaluate the expression of anti-apoptotic proteins and ROR1 using flow cytometry and western blot, PBMCs were treated with 25 \u03bcM of berberine for 24 hr. The expression levels of mir-21 were evaluated by real-time PCR. Examination of treated cells demonstrated that berberine decreased Bcl-2 and ROR1 levels. Although western blot results did not show any change in Bax as a pro-apoptotic protein, an increased Bax/Bcl-2 ratio indicated that mitochondrial pathway is involved in berberine-induced apoptosis of CLL cells. Interestingly, berberine could reduce the expression of miR-21 in comparison to the untreated group. Our findings describe some of the molecular mechanisms of berberine by decreasing Bcl-2, ROR1, and mir-21 which may be considered as a novel apoptosis inducer in CLL cells.", "doi": "10.1002/ptr.6945", "pmid": "33174291", "labels": [], "xrefs": [], "notes": [], "created": "2026-08-21T11:04:44.393Z", "modified": "2026-08-21T11:04:44.666Z"}]}