{"entity": "researcher", "timestamp": "2026-08-20T21:34:50.561Z", "family": "Doulabi", "given": "Ehsan Manouchehri", "initials": "EM", "orcid": "0000-0003-1639-2267", "affiliations": ["Department of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala University, Uppsala, Sweden."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/ad12164c3f004575b93f26cf83df40eb.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/ad12164c3f004575b93f26cf83df40eb"}}, "publications": [{"entity": "publication", "iuid": "304521984beb455dbb21d9bfe9b081b7", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/304521984beb455dbb21d9bfe9b081b7.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/304521984beb455dbb21d9bfe9b081b7"}}, "title": "Surface protein profiling of prostate-derived extracellular vesicles by mass spectrometry and proximity assays.", "authors": [{"family": "Manouchehri Doulabi", "given": "Ehsan", "initials": "E", "orcid": "0000-0003-1639-2267", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ad12164c3f004575b93f26cf83df40eb.json"}}, {"family": "Fredolini", "given": "Claudia", "initials": "C"}, {"family": "Gallini", "given": "Radiosa", "initials": "R", "orcid": "0000-0002-2709-3337", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9f5efcff1f4d469e987921b3ba4ecefd.json"}}, {"family": "L\u00f6f", "given": "Liza", "initials": "L"}, {"family": "Shen", "given": "Qiujin", "initials": "Q"}, {"family": "Ikebuchi", "given": "Ryoyo", "initials": "R"}, {"family": "Dubois", "given": "Louise", "initials": "L"}, {"family": "Azimi", "given": "Alireza", "initials": "A"}, {"family": "Loudig", "given": "Olivier", "initials": "O"}, {"family": "Gabrielsson", "given": "Susanne", "initials": "S", "orcid": "0000-0003-1771-1346", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4813fa3ad1ba45e28376583c64093a47.json"}}, {"family": "Landegren", "given": "Ulf", "initials": "U", "orcid": "0000-0002-7820-1000", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8749cdcda45a43d8bb2df04b0b211f36.json"}}, {"family": "Larsson", "given": "Anders", "initials": "A", "orcid": "0000-0003-3161-0402", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5b44e49c49f644cfa3348bcc4bb0080f.json"}}, {"family": "Bergquist", "given": "Jonas", "initials": "J", "orcid": "0000-0002-4597-041X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a27dc0277ccf4b94960dcbdc0e655e30.json"}}, {"family": "Kamali-Moghaddam", "given": "Masood", "initials": "M", "orcid": "0000-0002-1303-2218", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/70fceb2370d74e39a8c26d17963981cc.json"}}], "type": "journal article", "published": "2022-12-22", "journal": {"title": "Commun Biol", "issn": "2399-3642", "volume": "5", "issue": "1", "pages": "1402", "issn-l": "2399-3642"}, "abstract": "Extracellular vesicles (EVs) are mediators of intercellular communication and a promising class of biomarkers. Surface proteins of EVs play decisive roles in establishing a connection with recipient cells, and they are putative targets for diagnostic assays. Analysis of the surface proteins can thus both illuminate the biological functions of EVs and help identify potential biomarkers. We developed a strategy combining high-resolution mass spectrometry (HRMS) and proximity ligation assays (PLA) to first identify and then validate surface proteins discovered on EVs. We applied our workflow to investigate surface proteins of small EVs found in seminal fluid (SF-sEV). We identified 1,014 surface proteins and verified the presence of a subset of these on the surface of SF-sEVs. Our work demonstrates a general strategy for deep analysis of EVs' surface proteins across patients and pathological conditions, proceeding from unbiased screening by HRMS to ultra-sensitive targeted analyses via PLA.", "doi": "10.1038/s42003-022-04349-x", "pmid": "36550367", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC9780212"}, {"db": "pii", "key": "10.1038/s42003-022-04349-x"}], "notes": [], "created": "2026-08-20T09:25:13.924Z", "modified": "2026-08-20T09:25:14.135Z"}, {"entity": "publication", "iuid": "d449f0a4fad642e59027b725b02ef124", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/d449f0a4fad642e59027b725b02ef124.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/d449f0a4fad642e59027b725b02ef124"}}, "title": "Platelet-Derived PDGFB Promotes Recruitment of Cancer-Associated Fibroblasts, Deposition of Extracellular Matrix and Tgf\u03b2 Signaling in the Tumor Microenvironment.", "authors": [{"family": "Zhang", "given": "Yanyu", "initials": "Y", "orcid": "0000-0001-9939-1838", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/33cec15d087243bcb0a9b6ff967a8a05.json"}}, {"family": "Manouchehri Doulabi", "given": "Ehsan", "initials": "E", "orcid": "0000-0003-1639-2267", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ad12164c3f004575b93f26cf83df40eb.json"}}, {"family": "Herre", "given": "Melanie", "initials": "M"}, {"family": "Cedervall", "given": "Jessica", "initials": "J"}, {"family": "Qiao", "given": "Qi", "initials": "Q"}, {"family": "Miao", "given": "Zuoxiu", "initials": "Z"}, {"family": "Hamidi", "given": "Anahita", "initials": "A", "orcid": "0000-0002-5445-2446", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c9d2a9063065410385248b810cc9790f.json"}}, {"family": "Hellman", "given": "Lars", "initials": "L", "orcid": "0000-0003-1459-3815", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d8880fe8eb1847faba04190c7f9a76cf.json"}}, {"family": "Kamali-Moghaddam", "given": "Masood", "initials": "M", "orcid": "0000-0002-1303-2218", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/70fceb2370d74e39a8c26d17963981cc.json"}}, {"family": "Olsson", "given": "Anna-Karin", "initials": "AK"}], "type": "journal article", "published": "2022-04-12", "journal": {"title": "Cancers (Basel)", "issn": "2072-6694", "volume": "14", "issue": "8", "issn-l": "2072-6694"}, "abstract": "Platelets constitute a major reservoir of platelet-derived growth factor B (PDGFB) and are continuously activated in the tumor microenvironment, exposing tumors to the plethora of growth factors contained in platelet granules. To address the specific role of platelet-derived PDGFB in the tumor microenvironment, we have created a mouse model with conditional knockout of PDGFB in platelets (pl-PDGFB KO). Lack of PDGFB in platelets resulted in 10-fold lower PDGFB concentration in the tumor microenvironment, fewer cancer-associated fibroblasts and reduced deposition of the extracellular matrix (ECM) molecules fibronectin and collagen I in the orthotopic RIP1-Tag2 model for pancreatic neuroendocrine cancer. Myosin light chain phosphorylation, promoting cell contraction and, consequently, the mechano-induced release of active transforming growth factor (TGF) \u03b2 from extracellular compartments, was reduced in tumors from pl-PDGFB KO mice. In agreement, TGF\u03b2 signaling, measured as phosphorylated Smad2, was significantly hampered in tumors from mice lacking PDGFB in their platelets, providing a plausible explanation for the reduced deposition of extracellular matrix. These findings indicate a major contribution of platelet-derived PDGFB to a malignant transformation of the tumor microenvironment and address for the first time the role of PDGFB released specifically from platelets in the remodeling of the ECM in tumors.", "doi": "10.3390/cancers14081947", "pmid": "35454853", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC9024906"}, {"db": "pii", "key": "cancers14081947"}], "notes": [], "created": "2026-08-20T13:39:45.836Z", "modified": "2026-08-20T13:39:46.014Z"}, {"entity": "publication", "iuid": "ff6aff99d17f4c6385488d898ce4794a", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/ff6aff99d17f4c6385488d898ce4794a.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/ff6aff99d17f4c6385488d898ce4794a"}}, "title": "Image-based high-throughput mapping of TGF-\u03b2-induced phosphocomplexes at a single-cell level.", "authors": [{"family": "L\u00f6nn", "given": "Peter", "initials": "P", "orcid": "0000-0002-2405-9010", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/7fad118bf4d946889aac3349868656fa.json"}}, {"family": "Al-Amin", "given": "Rasel A", "initials": "RA", "orcid": "0000-0002-0762-9034", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/cb1973f718214a19a5dc787b443566df.json"}}, {"family": "Doulabi", "given": "Ehsan Manouchehri", "initials": "EM", "orcid": "0000-0003-1639-2267", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ad12164c3f004575b93f26cf83df40eb.json"}}, {"family": "Heldin", "given": "Johan", "initials": "J", "orcid": "0000-0002-0915-5303", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0c54a4210f844ab193a951c020d7406b.json"}}, {"family": "Gallini", "given": "Radiosa", "initials": "R"}, {"family": "Bj\u00f6rkesten", "given": "Johan", "initials": "J"}, {"family": "Oelrich", "given": "Johan", "initials": "J"}, {"family": "Kamali-Moghaddam", "given": "Masood", "initials": "M", "orcid": "0000-0002-1303-2218", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/70fceb2370d74e39a8c26d17963981cc.json"}}, {"family": "Landegren", "given": "Ulf", "initials": "U", "orcid": "0000-0002-7820-1000", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8749cdcda45a43d8bb2df04b0b211f36.json"}}], "type": "journal article", "published": "2021-11-12", "journal": {"title": "Commun Biol", "issn": "2399-3642", "volume": "4", "issue": "1", "pages": "1284", "issn-l": "2399-3642"}, "abstract": "Protein interactions and posttranslational modifications orchestrate cellular responses to e.g. cytokines and drugs, but it has been difficult to monitor these dynamic events in high-throughput. Here, we describe a semi-automated system for large-scale in situ proximity ligation assays (isPLA), combining isPLA in microtiter wells with automated microscopy and computer-based image analysis. Phosphorylations and interactions are digitally recorded along with subcellular morphological features. We investigated TGF-\u03b2-responsive Smad2 linker phosphorylations and complex formations over time and across millions of individual cells, and we relate these events to cell cycle progression and local cell crowding via measurements of DNA content and nuclear size of individual cells, and of their relative positions. We illustrate the suitability of this protocol to screen for drug effects using phosphatase inhibitors. Our approach expands the scope for image-based single cell analyses by combining observations of protein interactions and modifications with morphological details of individual cells at high throughput.", "doi": "10.1038/s42003-021-02798-4", "pmid": "34773084", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC8590043"}, {"db": "pii", "key": "10.1038/s42003-021-02798-4"}], "notes": [], "created": "2026-08-20T09:25:02.715Z", "modified": "2026-08-20T09:25:02.991Z"}, {"entity": "publication", "iuid": "183b650ab3f94e7d94bb58ef653653b8", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/183b650ab3f94e7d94bb58ef653653b8.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/183b650ab3f94e7d94bb58ef653653b8"}}, "title": "Developing an Electrochemical Biosensor for the Detection of Hemagglutinin Protein of Influenza A Virus Subtype H1N1 in Artificial Saliva", "authors": [{"family": "Torres-M\u00e9ndez", "given": "Carlos", "initials": "C"}, {"family": "Ellamathy", "given": "Jayendra", "initials": "J"}, {"family": "Mascarenhas", "given": "Maria Ines", "initials": "MI"}, {"family": "Liu", "given": "Yifan", "initials": "Y"}, {"family": "Gkountana", "given": "Georgia Vasiliki", "initials": "GV"}, {"family": "K\u00fchne", "given": "Patrizia", "initials": "P"}, {"family": "Sebasti\u00e1n", "given": "Javier", "initials": "J"}, {"family": "Jovanovic", "given": "Ivana", "initials": "I"}, {"family": "Bern", "given": "David", "initials": "D"}, {"family": "Nandi", "given": "Sharmilee", "initials": "S"}, {"family": "L\u00fcftner", "given": "Maike", "initials": "M"}, {"family": "Langwallner", "given": "Viktoria", "initials": "V"}, {"family": "Lysandrou", "given": "Maria", "initials": "M"}, {"family": "Taylor", "given": "Sam", "initials": "S"}, {"family": "Martinovic", "given": "Klara", "initials": "K"}, {"family": "Atif", "given": "Abdul Raouf", "initials": "AR"}, {"family": "Manouchehri Doulabi", "given": "Ehsan", "initials": "E", "orcid": "0000-0003-1639-2267", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ad12164c3f004575b93f26cf83df40eb.json"}}, {"family": "Kamali-Moghaddam", "given": "Masood", "initials": "M"}, {"family": "Mestres", "given": "Gemma", "initials": "G"}], "type": "proceedings-article", "published": "2021-06-30", "journal": {"pages": "80", "issn-l": null}, "abstract": null, "doi": "10.3390/csac2021-10477", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T13:40:28.541Z", "modified": "2026-08-20T13:40:28.586Z"}]}