{"entity": "researcher", "timestamp": "2026-03-16T23:56:35.767Z", "family": "Globisch", "given": "Daniel", "initials": "D", "orcid": "0000-0001-5288-6294", "affiliations": [], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/ab9e8721bc314e54acc364ac2b3d52a5.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/ab9e8721bc314e54acc364ac2b3d52a5"}}, "publications": [{"entity": "publication", "iuid": "1348892bd8d54a00abf4f9cfcf31972d", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/1348892bd8d54a00abf4f9cfcf31972d.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/1348892bd8d54a00abf4f9cfcf31972d"}}, "title": "Noninvasive Urine Biomarker Lateral Flow Immunoassay for Monitoring Active Onchocerciasis.", "authors": [{"family": "Shirey", "given": "Ryan J", "initials": "RJ", "orcid": "0000-0002-6715-5328", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1ef357f337ce47eda8d41b696b460675.json"}}, {"family": "Globisch", "given": "Daniel", "initials": "D", "orcid": "0000-0002-4526-5788", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/2cd75219d6894d6b851e202eaa40fc5f.json"}}, {"family": "Eubanks", "given": "Lisa M", "initials": "LM", "orcid": "0000-0001-5288-6294", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ab9e8721bc314e54acc364ac2b3d52a5.json"}}, {"family": "Hixon", "given": "Mark S", "initials": "MS", "orcid": "0000-0002-3525-0654", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3d12e96e530d428cb20ab656adbe206c.json"}}, {"family": "Janda", "given": "Kim D", "initials": "KD", "orcid": "0000-0001-6759-4227", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c892d0aa25ec48199d2d1df60135ec92.json"}}], "type": "journal article", "published": "2018-10-12", "journal": {"title": "ACS Infect Dis", "issn": "2373-8227", "issn-l": "2373-8227", "volume": "4", "issue": "10", "pages": "1423-1431"}, "abstract": "The parasitic disease onchocerciasis is the second leading cause of preventable blindness, afflicting more than 18 million people worldwide. Despite an available treatment, ivermectin, and control efforts by the World Health Organization, onchocerciasis remains a burden in many regions. With an estimated 120 million people living in areas at risk of infection, efforts are now shifting from prevention to surveillance and elimination. The lack of a robust, point-of-care diagnostic for an active Onchocerca infection has been a limiting factor in these efforts. Previously, we reported the discovery of the biomarker N-acetyl-tyramine- O-glucuronide (NATOG) in human urine samples and its ability to track treatment progression between medicated patients relative to placebo; we also established its capability to monitor disease burden in a jird model. NATOG is a human-produced metabolite of tyramine, which itself is produced as a nematode neurotransmitter. The ability of NATOG to distinguish between active and past infection overcomes the limitations of antibody biomarkers and PCR methodologies. Lateral flow immunoassay (LFIA) diagnostics offer the versatility and simplicity to be employed in the field and are inexpensive enough to be utilized in large-scale screening efforts. Herein, we report the development and assessment of a NATOG-based urine LFIA for onchocerciasis, which accurately identified 85% of analyzed patient samples ( N = 27).", "doi": "10.1021/acsinfecdis.8b00163", "pmid": "30141624", "labels": {"Daniel Globisch": null, "SciLifeLab Fellow": null}, "xrefs": [{"db": "pmc", "key": "PMC6189908"}], "notes": [], "created": "2020-09-28T07:32:41.242Z", "modified": "2022-11-04T11:32:17.207Z"}, {"entity": "publication", "iuid": "64bdc734f5204131b7df9ceabf38110d", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/64bdc734f5204131b7df9ceabf38110d.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/64bdc734f5204131b7df9ceabf38110d"}}, "title": "Validation of onchocerciasis biomarker N-acetyltyramine-O-glucuronide (NATOG).", "authors": [{"family": "Globisch", "given": "Daniel", "initials": "D", "orcid": "0000-0001-5288-6294", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ab9e8721bc314e54acc364ac2b3d52a5.json"}}, {"family": "Eubanks", "given": "Lisa M", "initials": "LM"}, {"family": "Shirey", "given": "Ryan J", "initials": "RJ"}, {"family": "Pfarr", "given": "Kenneth M", "initials": "KM"}, {"family": "Wanji", "given": "Samuel", "initials": "S"}, {"family": "Debrah", "given": "Alexander Y", "initials": "AY"}, {"family": "Hoerauf", "given": "Achim", "initials": "A"}, {"family": "Janda", "given": "Kim D", "initials": "KD"}], "type": "journal article", "published": "2017-08-01", "journal": {"title": "Bioorg. Med. Chem. Lett.", "issn": "1464-3405", "issn-l": "0960-894X", "volume": "27", "issue": "15", "pages": "3436-3440"}, "abstract": "The Neglected Tropical Disease onchocerciasis is a parasitic disease. Despite many control programmes by the World Health Organization (WHO), large communities in West and Central Africa are still affected. Besides logistic challenges during biannual mass drug administration, the lack of a robust, point-of-care diagnostic is limiting successful eradication of onchocerciasis. Towards the implementation of a non-invasive and point-of-care diagnostic, we have recently reported the discovery of the biomarker N-acetyltyramine-O-glucuronide (NATOG) in human urine samples using a metabolomics-mining approach. NATOG's biomarker value was enhanced during an investigation in a rodent model. Herein, we further detail the specificity of NATOG in active onchocerciasis infections as well as the co-infecting parasites Loa loa and Mansonella perstans. Our results measured by liquid chromatography coupled with mass spectrometry (LC-MS) reveal elevated NATOG values in mono- and co-infection samples only in the presence of the nematode Onchocerca volvulus. Metabolic pathway investigation of l-tyrosine/tyramine in all investigated nematodes uncovered an important link between the endosymbiotic bacterium Wolbachia and O. volvulus for the biosynthesis of NATOG. Based on these extended studies, we suggest NATOG as a biomarker for tracking active onchocerciasis infections and provide a threshold concentration value of NATOG for future diagnostic tool development.", "doi": "10.1016/j.bmcl.2017.05.082", "pmid": "28600214", "labels": {"Affiliated researcher": null, "Daniel Globisch": null, "SciLifeLab Fellow": null}, "xrefs": [{"db": "pii", "key": "S0960-894X(17)30581-4"}, {"db": "pmc", "key": "PMC5510726"}], "notes": [], "created": "2018-12-03T14:43:16.460Z", "modified": "2022-11-04T11:32:18.618Z"}]}