{"entity": "researcher", "timestamp": "2026-08-20T20:49:10.675Z", "family": "\u00d6sterroos", "given": "Albin", "initials": "A", "orcid": "0000-0001-8749-7299", "affiliations": ["Department of Medical Sciences, Uppsala University Hospital, Uppsala, Sweden."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/aafab8cd17e94d52a3cdeaf86d3acfa6.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/aafab8cd17e94d52a3cdeaf86d3acfa6"}}, "publications": [{"entity": "publication", "iuid": "b14473323e934321b8443c64c0102100", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/b14473323e934321b8443c64c0102100.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/b14473323e934321b8443c64c0102100"}}, "title": "Delineating functional and molecular impact of ex vivo sample handling in precision medicine.", "authors": [{"family": "Struyf", "given": "Nona", "initials": "N", "orcid": "0000-0002-6975-0753", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/be1087023d40465ca8133ea9845cd968.json"}}, {"family": "\u00d6sterroos", "given": "Albin", "initials": "A", "orcid": "0000-0001-8749-7299", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/aafab8cd17e94d52a3cdeaf86d3acfa6.json"}}, {"family": "Vesterlund", "given": "Mattias", "initials": "M", "orcid": "0000-0001-9471-6592", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e443cdb01cc44a7fa786941b5c0b62d6.json"}}, {"family": "Arnroth", "given": "Cornelia", "initials": "C"}, {"family": "James", "given": "Tojo", "initials": "T"}, {"family": "Sunandar", "given": "Stephanie", "initials": "S"}, {"family": "Mermelekas", "given": "Georgios", "initials": "G"}, {"family": "Bohlin", "given": "Anna", "initials": "A"}, {"family": "Hamberg Levedahl", "given": "Kerstin", "initials": "K"}, {"family": "Bengtz\u00e9n", "given": "Sofia", "initials": "S"}, {"family": "Jafari", "given": "Rozbeh", "initials": "R", "orcid": "0000-0002-3396-4709", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/95546f158dde40709fbfbb822a8f89c5.json"}}, {"family": "Orre", "given": "Lukas M", "initials": "LM", "orcid": "0000-0002-0384-1003", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e290f7cb5dfd49eb93e63cb704459516.json"}}, {"family": "Lehti\u00f6", "given": "Janne", "initials": "J"}, {"family": "Lehmann", "given": "S\u00f6ren", "initials": "S"}, {"family": "\u00d6stling", "given": "P\u00e4ivi", "initials": "P"}, {"family": "Kallioniemi", "given": "Olli", "initials": "O"}, {"family": "Seashore-Ludlow", "given": "Brinton", "initials": "B", "orcid": "0000-0001-8658-5967", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8a8ea4c3fdb14de1b651d0367b0a70a4.json"}}, {"family": "Erkers", "given": "Tom", "initials": "T", "orcid": "0000-0002-2754-6287", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4a1539d1d6a944eb882fdb80eb450706.json"}}], "type": "journal article", "published": "2024-02-19", "journal": {"title": "NPJ Precis Oncol", "issn": "2397-768X", "volume": "8", "issue": "1", "pages": "38", "issn-l": null}, "abstract": "Consistent handling of samples is crucial for achieving reproducible molecular and functional testing results in translational research. Here, we used 229 acute myeloid leukemia (AML) patient samples to assess the impact of sample handling on high-throughput functional drug testing, mass spectrometry-based proteomics, and flow cytometry. Our data revealed novel and previously described changes in cell phenotype and drug response dependent on sample biobanking. Specifically, myeloid cells with a CD117 (c-KIT) positive phenotype decreased after biobanking, potentially distorting cell population representations and affecting drugs targeting these cells. Additionally, highly granular AML cell numbers decreased after freezing. Secondly, protein expression levels, as well as sensitivity to drugs targeting cell proliferation, metabolism, tyrosine kinases (e.g., JAK, KIT, FLT3), and BH3 mimetics were notably affected by biobanking. Moreover, drug response profiles of paired fresh and frozen samples showed that freezing samples can lead to systematic errors in drug sensitivity scores. While a high correlation between fresh and frozen for the entire drug library was observed, freezing cells had a considerable impact at an individual level, which could influence outcomes in translational studies. Our study highlights conditions where standardization is needed to improve reproducibility, and where validation of data generated from biobanked cohorts may be particularly important.", "doi": "10.1038/s41698-024-00528-7", "pmid": "38374206", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC10876937"}, {"db": "pii", "key": "10.1038/s41698-024-00528-7"}], "notes": [], "created": "2026-08-20T09:24:34.463Z", "modified": "2026-08-20T09:24:34.644Z"}, {"entity": "publication", "iuid": "90a7c6fc6eec4e15a7dd35e4ddc0754b", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/90a7c6fc6eec4e15a7dd35e4ddc0754b.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/90a7c6fc6eec4e15a7dd35e4ddc0754b"}}, "title": "Somatic Exonic Deletions in RUNX1 Constitutes a Novel Recurrent Genomic Abnormality in Acute Myeloid Leukemia.", "authors": [{"family": "Eriksson", "given": "Anna", "initials": "A", "orcid": "0000-0002-8853-1863", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d37bf77253344a92968b4adb46c6f98c.json"}}, {"family": "Engvall", "given": "Marie", "initials": "M", "orcid": "0000-0002-7394-9191", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6107a9ca9ba842be9054a00c747b47a8.json"}}, {"family": "Mathot", "given": "Lucy", "initials": "L", "orcid": "0000-0002-2990-2038", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/458c07a1748d4ce0ac5cff3d6836f350.json"}}, {"family": "\u00d6sterroos", "given": "Albin", "initials": "A", "orcid": "0000-0001-8749-7299", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/aafab8cd17e94d52a3cdeaf86d3acfa6.json"}}, {"family": "Rippin", "given": "Martin", "initials": "M", "orcid": "0000-0003-4362-0122", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/06ef473951064c39ba0fbe0184244323.json"}}, {"family": "Cavelier", "given": "Lucia", "initials": "L", "orcid": "0009-0003-8195-370X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/aa2064616ff6495cb32d646823f0f8fc.json"}}, {"family": "Ladenvall", "given": "Claes", "initials": "C", "orcid": "0000-0002-7501-6598", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1cfe5cbb5d454552bdcbc464238f88fb.json"}}, {"family": "Baliakas", "given": "Panagiotis", "initials": "P", "orcid": "0000-0002-5634-7156", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/2a31997db1ee4ef1aaff75e423b8ccfb.json"}}], "type": "research support, non-u.s. gov't", "published": "2023-08-01", "journal": {"title": "Clin. Cancer Res.", "issn": "1557-3265", "volume": "29", "issue": "15", "pages": "2826-2834", "issn-l": "1078-0432"}, "abstract": "In acute myeloid leukemia (AML), somatic mutations (commonly missense, nonsense, and frameshift indels) in RUNX1 are associated with a dismal clinical outcome. Inherited RUNX1 mutations cause familial platelet disorder. As approximately 5%-10% of germline RUNX1 mutations are large exonic deletions, we hypothesized that such exonic RUNX1 aberrations may also be acquired during the development of AML.\n\nSixty patients with well-characterized AML were analyzed with multiplex ligation-dependent probe amplification (n = 60), microarray (n = 11), and/or whole-genome sequencing (n = 8).\n\nIn total, 25 (42% of the cohort) RUNX1-aberrant patients (defined by the presence of classical mutations and/or exonic deletions) were identified. Sixteen patients (27%) carried only exonic deletions, 5 (8%) carried classical mutations, and 4 (7%) carried both exonic deletions and mutations. No significant difference was observed between patients with classical RUNX1 mutations and RUNX1 exonic deletions in median overall survival (OS, 53.1 vs. 38.8 months, respectively, P = 0.63). When applying the European Leukemia Net (ELN) classification including the RUNX1-aberrant group, 20% of the patients initially stratified as intermediate-risk (5% of the whole cohort) were reassigned to the high-risk group, which improved the performance of ELN classification regarding OS between intermediate- and high-risk groups (18.9 vs. 9.6 months, P = 0.09).\n\nSomatic RUNX1 exonic deletions constitute a novel recurrent aberration in AML. Our findings have important clinical implications regarding AML classification, risk stratification, and treatment decision. Moreover, they argue in favor of further investigating such genomic aberrations not only in RUNX1 but also in other genes implicated in cancer biology and management. See related commentary by Chakraborty and Stengel, p. 2742.", "doi": "10.1158/1078-0432.CCR-23-0122", "pmid": "37022349", "labels": [], "xrefs": [{"db": "pii", "key": "725154"}], "notes": [], "created": "2026-08-20T12:12:14.173Z", "modified": "2026-08-20T12:12:14.475Z"}]}