{"entity": "researcher", "timestamp": "2026-03-10T03:50:27.017Z", "family": "Ek", "given": "Viktor", "initials": "V", "orcid": "0000-0002-5039-8163", "affiliations": [], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/a80b49fa9bd9407a8b806355e89ca201.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/a80b49fa9bd9407a8b806355e89ca201"}}, "publications": [{"entity": "publication", "iuid": "7a1f2bacf6804dfdaeadb202cb4f6f93", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/7a1f2bacf6804dfdaeadb202cb4f6f93.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/7a1f2bacf6804dfdaeadb202cb4f6f93"}}, "title": "High-efficiency transfection of<i>Acanthamoeba castellanii<\/i>using a cationic polymer", "authors": [{"family": "Moreno", "given": "Ana\u00edsa B", "initials": "AB", "orcid": "0000-0002-3229-4029", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/22821af48ff64059b3fa92e8acacd0c9.json"}}, {"family": "Ek", "given": "Viktor", "initials": "V", "orcid": "0000-0002-5039-8163", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a80b49fa9bd9407a8b806355e89ca201.json"}}, {"family": "Eriksson", "given": "Jens", "initials": "J", "orcid": "0000-0002-8945-2665", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/fe04815d524b48c2ba80c094c629362f.json"}}, {"family": "Sellin", "given": "Mikael E", "initials": "ME", "orcid": "0000-0002-8355-0803", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/559c18ec87d64318a0afc6267ea879e2.json"}}, {"family": "Guy", "given": "Lionel", "initials": "L", "orcid": "0000-0001-8354-2398", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/93e170c891c742838951a1fc8b50d223.json"}}], "type": "posted-content", "published": "2022-12-01", "journal": {"issn-l": null}, "abstract": null, "doi": "10.1101/2022.12.01.518696", "pmid": null, "labels": {"SciLifeLab Fellow": null, "Mikael Sellin": null}, "xrefs": [], "notes": [], "created": "2023-05-29T12:43:00.839Z", "modified": "2025-12-04T16:56:02.523Z"}, {"entity": "publication", "iuid": "30796333893a4c4fb91ff06d1c2b2543", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/30796333893a4c4fb91ff06d1c2b2543.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/30796333893a4c4fb91ff06d1c2b2543"}}, "title": "A motile doublet form of Salmonella Typhimurium diversifies target search behavior at the epithelial surface.", "authors": [{"family": "Ek", "given": "Viktor", "initials": "V", "orcid": "0000-0002-5039-8163", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a80b49fa9bd9407a8b806355e89ca201.json"}}, {"family": "Fattinger", "given": "Stefan A", "initials": "SA", "orcid": "0000-0003-4899-4414", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/2a3891756a0e46b7be8494556da9254f.json"}}, {"family": "Florbrant", "given": "Alexandra", "initials": "A", "orcid": "0000-0002-1630-4442", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/21b09621be1d4fe5a3c956360234b99d.json"}}, {"family": "Hardt", "given": "Wolf-Dietrich", "initials": "WD", "orcid": "0000-0002-9892-6420", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1049556a73b84d258e26a84382fe5870.json"}}, {"family": "Di Martino", "given": "Maria Letizia", "initials": "ML", "orcid": "0000-0002-9491-4000", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ccaa06e9e2d441589299b7e33ee55034.json"}}, {"family": "Eriksson", "given": "Jens", "initials": "J", "orcid": "0000-0002-8945-2665", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/fe04815d524b48c2ba80c094c629362f.json"}}, {"family": "Sellin", "given": "Mikael E", "initials": "ME", "orcid": "0000-0002-8355-0803", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/559c18ec87d64318a0afc6267ea879e2.json"}}], "type": "journal article", "published": "2022-05-00", "journal": {"title": "Mol. Microbiol.", "issn": "1365-2958", "volume": "117", "issue": "5", "pages": "1156-1172", "issn-l": "0950-382X"}, "abstract": "The behaviors of infectious bacteria are commonly studied in bulk. This is effective to define the general properties of a given isolate, but insufficient to resolve subpopulations and unique single-microbe behaviors within the bacterial pool. We here employ microscopy to study single-bacterium characteristics among Salmonella enterica serovar Typhimurium (S.Tm), as they prepare for and launch invasion of epithelial host cells. We find that during the bacterial growth cycle, S.Tm populations switch gradually from fast planktonic growth to a host cell-invasive phenotype, characterized by flagellar motility and expression of the Type-three-secretion-system-1. The indistinct nature of this shift leads to the establishment of a transient subpopulation of S.Tm \"doublets\"-waist-bearing bacteria anticipating cell division-which simultaneously express host cell invasion machinery. In epithelial cell culture infections, these S.Tm doublets outperform their \"singlet\" brethren and represent a hyperinvasive subpopulation. Atop both glass and enteroid-derived monolayers, doublets swim along markedly straighter trajectories than singlets, thereby diversifying search patterns and improving the surface exploration capacity of the total bacterial population. The straighter swimming, combined with an enhanced cell-adhesion propensity, suffices to account for the hyperinvasive doublet phenotype. This work highlights bacterial cell length heterogeneity as a key determinant of target search patterns atop epithelia.", "doi": "10.1111/mmi.14898", "pmid": "35332598", "labels": {"SciLifeLab Fellow": null, "Mikael Sellin": null}, "xrefs": [{"db": "pmc", "key": "PMC9325389"}], "notes": [], "created": "2022-12-04T10:59:59.629Z", "modified": "2022-12-04T10:59:59.686Z"}, {"entity": "publication", "iuid": "f229e19e6b83494a81fb7d790ab88618", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/f229e19e6b83494a81fb7d790ab88618.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/f229e19e6b83494a81fb7d790ab88618"}}, "title": "Salmonella Typhimurium discreet-invasion of the murine gut absorptive epithelium.", "authors": [{"family": "Fattinger", "given": "Stefan A", "initials": "SA"}, {"family": "B\u00f6ck", "given": "Desir\u00e9e", "initials": "D", "orcid": "0000-0001-8142-0629", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b1ba5760859a4340a83fca45b4e92d57.json"}}, {"family": "Di Martino", "given": "Maria Letizia", "initials": "ML", "orcid": "0000-0002-9491-4000", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ccaa06e9e2d441589299b7e33ee55034.json"}}, {"family": "Deuring", "given": "Sabrina", "initials": "S"}, {"family": "Samperio Ventayol", "given": "Pilar", "initials": "P", "orcid": "0000-0002-3726-7871", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e7647686922142a8b8bd8629142445fc.json"}}, {"family": "Ek", "given": "Viktor", "initials": "V", "orcid": "0000-0002-5039-8163", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a80b49fa9bd9407a8b806355e89ca201.json"}}, {"family": "Furter", "given": "Markus", "initials": "M", "orcid": "0000-0003-4073-2268", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/51f09daa062347adb84d145a0128c608.json"}}, {"family": "Kreibich", "given": "Saskia", "initials": "S"}, {"family": "Bosia", "given": "Francesco", "initials": "F", "orcid": "0000-0001-6021-7672", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e85e92f19bc14b3095cf38a05c639343.json"}}, {"family": "M\u00fcller-Hauser", "given": "Anna A", "initials": "AA", "orcid": "0000-0002-8031-2404", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/aa6eb65b570d4b6390871b05b6c824a6.json"}}, {"family": "Nguyen", "given": "Bidong D", "initials": "BD"}, {"family": "Rohde", "given": "Manfred", "initials": "M"}, {"family": "Pilhofer", "given": "Martin", "initials": "M", "orcid": "0000-0002-3649-3340", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5c78a3970a954249aa16c9e824813e0d.json"}}, {"family": "Hardt", "given": "Wolf-Dietrich", "initials": "WD", "orcid": "0000-0002-9892-6420", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1049556a73b84d258e26a84382fe5870.json"}}, {"family": "Sellin", "given": "Mikael E", "initials": "ME", "orcid": "0000-0002-8355-0803", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/559c18ec87d64318a0afc6267ea879e2.json"}}], "type": "journal article", "published": "2020-05-00", "journal": {"title": "PLoS Pathog", "issn": "1553-7374", "issn-l": "1553-7366", "volume": "16", "issue": "5", "pages": "e1008503"}, "abstract": "Salmonella enterica serovar Typhimurium (S.Tm) infections of cultured cell lines have given rise to the ruffle model for epithelial cell invasion. According to this model, the Type-Three-Secretion-System-1 (TTSS-1) effectors SopB, SopE and SopE2 drive an explosive actin nucleation cascade, resulting in large lamellipodia- and filopodia-containing ruffles and cooperative S.Tm uptake. However, cell line experiments poorly recapitulate many of the cell and tissue features encountered in the host's gut mucosa. Here, we employed bacterial genetics and multiple imaging modalities to compare S.Tm invasion of cultured epithelial cell lines and the gut absorptive epithelium in vivo in mice. In contrast to the prevailing ruffle-model, we find that absorptive epithelial cell entry in the mouse gut occurs through \"discreet-invasion\". This distinct entry mode requires the conserved TTSS-1 effector SipA, involves modest elongation of local microvilli in the absence of expansive ruffles, and does not favor cooperative invasion. Discreet-invasion preferentially targets apicolateral hot spots at cell-cell junctions and shows strong dependence on local cell neighborhood. This proof-of-principle evidence challenges the current model for how S.Tm can enter gut absorptive epithelial cells in their intact in vivo context.", "doi": "10.1371/journal.ppat.1008503", "pmid": "32365138", "labels": {"Mikael Sellin": null, "SciLifeLab Fellow": null}, "xrefs": [{"db": "pii", "key": "PPATHOGENS-D-20-00095"}, {"db": "pmc", "key": "PMC7224572"}], "notes": [], "created": "2020-11-24T14:44:13.211Z", "modified": "2022-11-04T11:32:15.048Z"}]}