{"entity": "researcher", "timestamp": "2026-08-22T07:48:42.672Z", "family": "Kinowaki", "given": "Yuko", "initials": "Y", "orcid": "0000-0001-8907-2569", "affiliations": ["Department of Comprehensive Pathology, Graduate School of Medicine and Dentistry, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8519, Japan."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/a64a21648e9b4b69b77e629c4477bcaf.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/a64a21648e9b4b69b77e629c4477bcaf"}}, "publications": [{"entity": "publication", "iuid": "4cc641d5252841fb8b89e9073413bf8f", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/4cc641d5252841fb8b89e9073413bf8f.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/4cc641d5252841fb8b89e9073413bf8f"}}, "title": "Spatial and Quantitative Analysis of Tumor-Associated Macrophages: Intratumoral CD163-/PD-L1+ TAMs as a Marker of Favorable Clinical Outcomes in Triple-Negative Breast Cancer.", "authors": [{"family": "Shinohara", "given": "Hajime", "initials": "H"}, {"family": "Kobayashi", "given": "Maki", "initials": "M"}, {"family": "Hayashi", "given": "Kumiko", "initials": "K"}, {"family": "Nogawa", "given": "Daichi", "initials": "D"}, {"family": "Asakawa", "given": "Ayaka", "initials": "A"}, {"family": "Ohata", "given": "Yae", "initials": "Y"}, {"family": "Kubota", "given": "Kazuishi", "initials": "K"}, {"family": "Takahashi", "given": "Hisashi", "initials": "H"}, {"family": "Yamada", "given": "Miyuki", "initials": "M"}, {"family": "Tokunaga", "given": "Masanori", "initials": "M"}, {"family": "Kinugasa", "given": "Yusuke", "initials": "Y"}, {"family": "Oda", "given": "Goshi", "initials": "G"}, {"family": "Nakagawa", "given": "Tsuyoshi", "initials": "T", "orcid": "0000-0003-1967-0445", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/09af77fdf8644b2595a6731f4982b1f3.json"}}, {"family": "Onishi", "given": "Iichiroh", "initials": "I"}, {"family": "Kinowaki", "given": "Yuko", "initials": "Y", "orcid": "0000-0001-8907-2569", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a64a21648e9b4b69b77e629c4477bcaf.json"}}, {"family": "Kurata", "given": "Morito", "initials": "M", "orcid": "0000-0002-1242-6709", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/78500882ea43486f825d924fd316bfba.json"}}, {"family": "Ohashi", "given": "Kenichi", "initials": "K"}, {"family": "Kitagawa", "given": "Masanobu", "initials": "M"}, {"family": "Yamamoto", "given": "Kouhei", "initials": "K", "orcid": "0000-0003-3883-4353", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e1149d1273964d048aeb009be9c5f098.json"}}], "type": "journal article", "published": "2022-10-31", "journal": {"title": "Int J Mol Sci", "issn": "1422-0067", "volume": "23", "issue": "21", "issn-l": null}, "abstract": "Tumor-associated macrophages (TAMs) and abnormalities in cancer cells affect cancer progression and response to therapy. TAMs are a major component of the tumor microenvironment (TME) in breast cancer, with their invasion affecting clinical outcomes. Programmed death-ligand 1 (PD-L1), a target of immune checkpoint inhibitors, acts as a suppressive signal for the surrounding immune system; however, its expression and effect on TAMs and the clinical outcome in breast cancer are unknown. In this study, we used high-throughput multiple immunohistochemistry to spatially and quantitatively analyze TAMs. We subjected 81 breast cancer specimens to immunostaining for CD68, CD163, PD-1, PD-L1, CD20, and pan-CK. In both stromal and intratumoral areas, the triple-negative subtype had significantly more CD68/CD163, CD68/PD-L1, and CD163/PD-L1 double-positive cells than the estrogen receptor (ER)/progesterone receptor (PR) subtype. Interestingly, a higher number of CD68+/PD-L1+/CK-/CD163- TAMs in the intratumoral area was correlated with a favorable recurrence rate (p = 0.048). These findings indicated that the specific subpopulation and localization of TAMs in the TME affect clinical outcomes in breast cancer.", "doi": "10.3390/ijms232113235", "pmid": "36362023", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC9656504"}, {"db": "pii", "key": "ijms232113235"}], "notes": [], "created": "2026-08-21T13:03:36.283Z", "modified": "2026-08-21T13:03:36.494Z"}]}