{"entity": "researcher", "timestamp": "2026-08-22T07:49:37.608Z", "family": "Tudoran", "given": "Oana", "initials": "O", "orcid": "0000-0001-5082-9341", "affiliations": ["Department of Genetics, Genomics and Experimental Pathology, The Oncology Institute \"Prof. Dr. I. Chiricuta\", 400015 Cluj-Napoca, Romania."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/9cf584d23e4042d1a4519fd0eb47d560.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/9cf584d23e4042d1a4519fd0eb47d560"}}, "publications": [{"entity": "publication", "iuid": "91dc7838bf8b4cb4aa99039bae7b75d0", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/91dc7838bf8b4cb4aa99039bae7b75d0.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/91dc7838bf8b4cb4aa99039bae7b75d0"}}, "title": "Assessing the Layer-by-Layer Assembly of Cellulose Nanofibrils and Polyelectrolytes in Pancreatic Tumor Spheroid Formation.", "authors": [{"family": "Abbasi Aval", "given": "Negar", "initials": "N", "orcid": "0000-0002-6544-9955", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f972e8b710be428299108c3459fd56c7.json"}}, {"family": "Lahchaichi", "given": "Ekeram", "initials": "E", "orcid": "0009-0005-3637-2513", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c7b007cf91af4fcf89c6dce2a7146706.json"}}, {"family": "Tudoran", "given": "Oana", "initials": "O", "orcid": "0000-0001-5082-9341", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9cf584d23e4042d1a4519fd0eb47d560.json"}}, {"family": "Fayazbakhsh", "given": "Farzaneh", "initials": "F"}, {"family": "Heuchel", "given": "Rainer", "initials": "R"}, {"family": "L\u00f6hr", "given": "Matthias", "initials": "M", "orcid": "0000-0002-7647-198X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1b209074b1e548ddad51414dab2d0b19.json"}}, {"family": "Pettersson", "given": "Torbj\u00f6rn", "initials": "T", "orcid": "0000-0002-5444-7276", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f324e73b0d1e491280bf0bb973610a69.json"}}, {"family": "Russom", "given": "Aman", "initials": "A"}], "type": "journal article", "published": "2023-11-15", "journal": {"title": "Biomedicines", "issn": "2227-9059", "volume": "11", "issue": "11", "issn-l": null}, "abstract": "Three-dimensional (3D) tumor spheroids are regarded as promising models for utilization as preclinical assessments of chemo-sensitivity. However, the creation of these tumor spheroids presents challenges, given that not all tumor cell lines are able to form consistent and regular spheroids. In this context, we have developed a novel layer-by-layer coating of cellulose nanofibril-polyelectrolyte bilayers for the generation of spheroids. This technique builds bilayers of cellulose nanofibrils and polyelectrolytes and is used here to coat two distinct 96-well plate types: nontreated/non-sterilized and Nunclon Delta. In this work, we optimized the protocol aimed at generating and characterizing spheroids on difficult-to-grow pancreatic tumor cell lines. Here, diverse parameters were explored, encompassing the bilayer count (five and ten) and multiple cell-seeding concentrations (10, 100, 200, 500, and 1000 cells per well), using four pancreatic tumor cell lines-KPCT, PANC-1, MiaPaCa-2, and CFPAC-I. The evaluation includes the quantification (number of spheroids, size, and morphology) and proliferation of the produced spheroids, as well as an assessment of their viability. Notably, our findings reveal a significant influence from both the number of bilayers and the plate type used on the successful formation of spheroids. The novel and simple layer-by-layer-based coating method has the potential to offer the large-scale production of spheroids across a spectrum of tumor cell lines.", "doi": "10.3390/biomedicines11113061", "pmid": "38002061", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC10669291"}, {"db": "pii", "key": "biomedicines11113061"}], "notes": [], "created": "2026-08-20T13:38:54.829Z", "modified": "2026-08-20T13:38:55.045Z"}]}