{"entity": "researcher", "timestamp": "2026-08-20T20:58:11.964Z", "family": "Mestres", "given": "Gemma", "initials": "G", "orcid": "0000-0001-7462-4236", "affiliations": ["Department of Materials Science and Engineering, Science for Life Laboratory, Uppsala University, Box 35, 751 03 Uppsala, Sweden."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/9b6944549b4b4480af1b441cd4c70f56.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/9b6944549b4b4480af1b441cd4c70f56"}}, "publications": [{"entity": "publication", "iuid": "e6eeb2c6963b420bb5e91d2d157e7466", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/e6eeb2c6963b420bb5e91d2d157e7466.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/e6eeb2c6963b420bb5e91d2d157e7466"}}, "title": "Experimental Characterization and Mathematical Modeling of the Adsorption of Proteins and Cells on Biomimetic Hydroxyapatite.", "authors": [{"family": "Atif", "given": "Abdul-Raouf", "initials": "AR"}, {"family": "La Cis", "given": "U\u01f5is", "initials": "U"}, {"family": "Engqvist", "given": "H\u00e5kan", "initials": "H"}, {"family": "Tenje", "given": "Maria", "initials": "M", "orcid": "0000-0002-1264-1337", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/bd13273274574923b354ca9c605efc8f.json"}}, {"family": "Bagheri", "given": "Shervin", "initials": "S"}, {"family": "Mestres", "given": "Gemma", "initials": "G", "orcid": "0000-0001-7462-4236", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9b6944549b4b4480af1b441cd4c70f56.json"}}], "type": "journal article", "published": "2022-01-11", "journal": {"title": "ACS Omega", "issn": "2470-1343", "volume": "7", "issue": "1", "pages": "908-920", "issn-l": "2470-1343"}, "abstract": "Biomaterial development is a long process consisting of multiple stages of design and evaluation within the context of both in vitro and in vivo testing. To streamline this process, mathematical and computational modeling displays potential as a tool for rapid biomaterial characterization, enabling the prediction of optimal physicochemical parameters. In this work, a Langmuir isotherm-based model was used to describe protein and cell adhesion on a biomimetic hydroxyapatite surface, both independently and in a one-way coupled system. The results indicated that increased protein surface coverage leads to improved cell adhesion and spread, with maximal protein coverage occurring within 48 h. In addition, the Langmuir model displayed a good fit with the experimental data. Overall, computational modeling is an exciting avenue that may lead to savings in terms of time and cost during the biomaterial development process.", "doi": "10.1021/acsomega.1c05540", "pmid": "35036755", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC8757448"}], "notes": [], "created": "2026-08-20T08:12:33.095Z", "modified": "2026-08-20T08:12:33.176Z"}, {"entity": "publication", "iuid": "7ff490b158194d58b60b2b19cdaae261", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/7ff490b158194d58b60b2b19cdaae261.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/7ff490b158194d58b60b2b19cdaae261"}}, "title": "Effect of the Addition Frequency of 5-Azacytidine in Both Micro- and Macroscale Cultures.", "authors": [{"family": "Kadekar", "given": "Sandeep", "initials": "S"}, {"family": "Barbe", "given": "Laurent", "initials": "L"}, {"family": "Stoddart", "given": "Martin", "initials": "M"}, {"family": "Varghese", "given": "Oommen P", "initials": "OP"}, {"family": "Tenje", "given": "Maria", "initials": "M"}, {"family": "Mestres", "given": "Gemma", "initials": "G", "orcid": "0000-0001-7462-4236", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9b6944549b4b4480af1b441cd4c70f56.json"}}], "type": "journal article", "published": "2021-02-00", "journal": {"title": "Cell Mol Bioeng", "issn": "1865-5025", "volume": "14", "issue": "1", "pages": "121-130", "issn-l": null}, "abstract": "Human mesenchymal stem cells (hMSCs) have a great clinical potential for tissue regeneration purposes due to its multilineage capability. Previous studies have reported that a single addition of 5-azacytidine (5-AzaC) causes the differentiation of hMSCs towards a myocardial lineage. The aim of this work was to evaluate the effect of 5-AzaC addition frequency on hMSCs priming (i.e., indicating an early genetic differentiation) using two culture environments.\n\nhMSCs were supplemented with 5-AzaC while cultured in well plates and in microfluidic chips. The impact of 5-AzaC concentration (10 and 20 \u03bcM) and addition frequency (once, daily or continuously), as well as of culture period (2 or 5 days) on the genetic upregulation of PPAR\u03b3 (adipocytes), PAX3 (myoblasts), SOX9 (chondrocytes) and RUNX2 (osteoblasts) was evaluated.\n\nDaily delivering 5-AzaC caused a higher upregulation of PPAR\u03b3, SOX9 and RUNX2 in comparison to a single dose delivery, both under static well plates and dynamic microfluidic cultures. A particularly high gene expression of PPAR\u03b3 (tenfold-change) could indicate priming of hMSCs towards adipocytes.\n\nBoth macro- and microscale cultures provided results with similar trends, where addition frequency of 5-AzaC was a crucial factor to upregulate several genes. Microfluidics technology was proven to be a suitable platform for the continuous delivery of a drug and could be used for screening purposes in tissue engineering research.", "doi": "10.1007/s12195-020-00654-9", "pmid": "33633814", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC7878657"}, {"db": "pii", "key": "654"}], "notes": [], "created": "2026-08-20T06:40:37.743Z", "modified": "2026-08-20T06:40:37.836Z"}]}