{"entity": "researcher", "timestamp": "2026-04-13T04:29:53.013Z", "family": "Ohata", "given": "Yae", "initials": "Y", "orcid": "0000-0002-5518-835X", "affiliations": ["Department of Medical Biochemistry and Microbiology, Science for Life Laboratory, Biomedical Center, Uppsala University, Sweden."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/97ecb5ad5b6a4aa69a4d84523cb84918.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/97ecb5ad5b6a4aa69a4d84523cb84918"}}, "publications": [{"entity": "publication", "iuid": "19c5c3cd794b4250bb2a6ff65d9665f1", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/19c5c3cd794b4250bb2a6ff65d9665f1.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/19c5c3cd794b4250bb2a6ff65d9665f1"}}, "title": "TGF\u03b2 selects for pro-stemness over pro-invasive phenotypes during cancer cell epithelial-mesenchymal transition.", "authors": [{"family": "Tsubakihara", "given": "Yutaro", "initials": "Y"}, {"family": "Ohata", "given": "Yae", "initials": "Y", "orcid": "0000-0002-5518-835X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/97ecb5ad5b6a4aa69a4d84523cb84918.json"}}, {"family": "Okita", "given": "Yukari", "initials": "Y", "orcid": "0000-0002-7279-4634", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/35f1dd6b5aa74ab9a53620c614680674.json"}}, {"family": "Younis", "given": "Shady", "initials": "S", "orcid": "0000-0002-4319-1738", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/cd2c4773dcf84b9eb1b82d19e5e2039c.json"}}, {"family": "Eriksson", "given": "Jens", "initials": "J", "orcid": "0000-0002-8945-2665", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/fe04815d524b48c2ba80c094c629362f.json"}}, {"family": "Sellin", "given": "Mikael E", "initials": "ME", "orcid": "0000-0002-8355-0803", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/559c18ec87d64318a0afc6267ea879e2.json"}}, {"family": "Ren", "given": "Jiang", "initials": "J"}, {"family": "Ten Dijke", "given": "Peter", "initials": "P", "orcid": "0000-0002-7234-342X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/2193ff2b5f924395ac6d4a9ddd53f20e.json"}}, {"family": "Miyazono", "given": "Kohei", "initials": "K", "orcid": "0000-0001-7341-0172", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/88b0a85433264e96a3c4f2e27ca0e3f7.json"}}, {"family": "Hikita", "given": "Atsuhiko", "initials": "A", "orcid": "0000-0001-9552-2976", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d8d3ea8a83164bb7af09a879dfe8c52f.json"}}, {"family": "Imamura", "given": "Takeshi", "initials": "T", "orcid": "0000-0001-5101-4304", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a694d49ba34d4aac8f6b73e21f0303e1.json"}}, {"family": "Kato", "given": "Mitsuyasu", "initials": "M", "orcid": "0000-0001-9905-2473", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/04d3edbf488a445583e31a14d7576aca.json"}}, {"family": "Heldin", "given": "Carl-Henrik", "initials": "CH", "orcid": "0000-0002-9508-896X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5bca6678f5eb4baf853fca6ed5f9b726.json"}}, {"family": "Moustakas", "given": "Aristidis", "initials": "A", "orcid": "0000-0001-9131-3827", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f11771bffabf4da6a2a5e2668c7c7c2d.json"}}], "type": "journal article", "published": "2022-06-00", "journal": {"title": "Mol Oncol", "issn": "1878-0261", "volume": "16", "issue": "12", "pages": "2330-2354", "issn-l": "1574-7891"}, "abstract": "Transforming growth factor \u03b2 (TGF\u03b2) induces epithelial-mesenchymal transition (EMT), which correlates with stemness and invasiveness. Mesenchymal-epithelial transition (MET) is induced by TGF\u03b2 withdrawal and correlates with metastatic colonization. Whether TGF\u03b2 promotes stemness and invasiveness simultaneously via EMT remains unclear. We established a breast cancer cell model expressing red fluorescent protein (RFP) under the E-cadherin promoter. In 2D cultures, TGF\u03b2 induced EMT, generating RFPlow cells with a mesenchymal transcriptome, and regained RFP, with an epithelial transcriptome, after MET induced by TGF\u03b2 withdrawal. RFPlow cells generated robust mammospheres, with epithelio-mesenchymal cell surface features. Mammospheres that were forced to adhere generated migratory cells, devoid of RFP, a phenotype which was inhibited by a TGF\u03b2 receptor kinase inhibitor. Further stimulation of RFPlow mammospheres with TGF\u03b2 suppressed the generation of motile cells, but enhanced mammosphere growth. Accordingly, mammary fat-pad-transplanted mammospheres, in the absence of exogenous TGF\u03b2 treatment, established lung metastases with evident MET (RFPhigh cells). In contrast, TGF\u03b2-treated mammospheres revealed high tumour-initiating capacity, but limited metastatic potential. Thus, the biological context of partial EMT and MET allows TGF\u03b2 to differentiate between pro-stemness and pro-invasive phenotypes.", "doi": "10.1002/1878-0261.13215", "pmid": "35348275", "labels": {"SciLifeLab Fellow": null, "Mikael Sellin": null}, "xrefs": [{"db": "pmc", "key": "PMC9208077"}, {"db": "RefSeq", "key": "E-MTAB-97509"}], "notes": [], "created": "2022-12-04T11:00:00.977Z", "modified": "2022-12-04T11:00:01.263Z"}]}