{"entity": "researcher", "timestamp": "2026-09-28T11:18:12.424Z", "family": "Hell", "given": "Stefan W", "initials": "SW", "orcid": "0000-0002-9638-5077", "affiliations": ["Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, G\u00f6ttingen, Germany. stefan.hell@mpibpc.mpg.de francisco.balzarotti@mpibpc.mpg.de.", "Department of Optical Nanoscopy, Max Planck Institute for Medical Research, Heidelberg, Germany.", "Optical Nanoscopy Division, German Cancer Research Center (DKFZ), Heidelberg, Germany."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/8f4913eaf69041e18df41d93ec48f2c2.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/8f4913eaf69041e18df41d93ec48f2c2"}}, "publications": [{"entity": "publication", "iuid": "22b4c561ac594b90bbde981a4030b033", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/22b4c561ac594b90bbde981a4030b033.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/22b4c561ac594b90bbde981a4030b033"}}, "title": "Mic60 exhibits a coordinated clustered distribution along and across yeast and mammalian mitochondria.", "authors": [{"family": "Stoldt", "given": "Stefan", "initials": "S"}, {"family": "Stephan", "given": "Till", "initials": "T"}, {"family": "Jans", "given": "Daniel C", "initials": "DC"}, {"family": "Br\u00fcser", "given": "Christian", "initials": "C"}, {"family": "Lange", "given": "Felix", "initials": "F"}, {"family": "Keller-Findeisen", "given": "Jan", "initials": "J", "orcid": "0000-0003-2753-0848", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/44bf49cfdf4f409ea97073a70c9e5eba.json"}}, {"family": "Riedel", "given": "Dietmar", "initials": "D"}, {"family": "Hell", "given": "Stefan W", "initials": "SW", "orcid": "0000-0002-9638-5077", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8f4913eaf69041e18df41d93ec48f2c2.json"}}, {"family": "Jakobs", "given": "Stefan", "initials": "S"}], "type": "journal article", "published": "2019-05-14", "journal": {"title": "Proc. Natl. Acad. Sci. U.S.A.", "issn": "1091-6490", "volume": "116", "issue": "20", "pages": "9853-9858", "issn-l": "0027-8424"}, "abstract": "Mitochondria are tubular double-membrane organelles essential for eukaryotic life. They form extended networks and exhibit an intricate inner membrane architecture. The MICOS (mitochondrial contact site and cristae organizing system) complex, crucial for proper architecture of the mitochondrial inner membrane, is localized primarily at crista junctions. Harnessing superresolution fluorescence microscopy, we demonstrate that Mic60, a subunit of the MICOS complex, as well as several of its interaction partners are arranged into intricate patterns in human and yeast mitochondria, suggesting an ordered distribution of the crista junctions. We show that Mic60 forms clusters that are preferentially localized in the inner membrane at two opposing sides of the mitochondrial tubules so that they form extended opposing distribution bands. These Mic60 distribution bands can be twisted, resulting in a helical arrangement. Focused ion beam milling-scanning electron microscopy showed that in yeast the twisting of the opposing distribution bands is echoed by the folding of the inner membrane. We show that establishment of the Mic60 distribution bands is largely independent of the cristae morphology. We suggest that Mic60 is part of an extended multiprotein interaction network that scaffolds mitochondria.", "doi": "10.1073/pnas.1820364116", "pmid": "31028145", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC6525524"}, {"db": "pii", "key": "1820364116"}], "notes": [], "created": "2026-09-23T09:16:19.652Z", "modified": "2026-09-23T09:16:19.736Z"}, {"entity": "publication", "iuid": "c1cb0e1577e843669268c51d85617973", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/c1cb0e1577e843669268c51d85617973.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/c1cb0e1577e843669268c51d85617973"}}, "title": "Nanometer resolution imaging and tracking of fluorescent molecules with minimal photon fluxes.", "authors": [{"family": "Balzarotti", "given": "Francisco", "initials": "F", "orcid": "0000-0003-1471-8120", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/aa561abf1a1f4bb9abda75213a02b41e.json"}}, {"family": "Eilers", "given": "Yvan", "initials": "Y", "orcid": "0000-0001-6209-4864", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e1ca635216f840228905234c10e0240e.json"}}, {"family": "Gwosch", "given": "Klaus C", "initials": "KC", "orcid": "0000-0002-8897-2335", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4d5899c1a6d646588e088b74c8a9e6e7.json"}}, {"family": "Gynn\u00e5", "given": "Arvid H", "initials": "AH", "orcid": "0000-0001-8087-7715", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a6ad0db3cd4e4c0cba5eff0e6d31ec81.json"}}, {"family": "Westphal", "given": "Volker", "initials": "V"}, {"family": "Stefani", "given": "Fernando D", "initials": "FD", "orcid": "0000-0002-3277-7215", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/92a9512559ab4cd5a4db6a4df3ee8f1c.json"}}, {"family": "Elf", "given": "Johan", "initials": "J", "orcid": "0000-0001-5522-1810", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/7001138c10bf4a6d84075057ba30c8b9.json"}}, {"family": "Hell", "given": "Stefan W", "initials": "SW", "orcid": "0000-0002-9638-5077", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8f4913eaf69041e18df41d93ec48f2c2.json"}}], "type": "journal article", "published": "2017-02-10", "journal": {"title": "Science (New York, N.Y.)", "issn": "1095-9203", "volume": "355", "issue": "6325", "pages": "606-612", "issn-l": "0036-8075"}, "abstract": "We introduce MINFLUX, a concept for localizing photon emitters in space. By probing the emitter with a local intensity minimum of excitation light, MINFLUX minimizes the fluorescence photons needed for high localization precision. In our experiments, 22 times fewer fluorescence photons are required as compared to popular centroid localization. In superresolution microscopy, MINFLUX attained ~1-nm precision, resolving molecules only 6 nanometers apart. MINFLUX tracking of single fluorescent proteins increased the temporal resolution and the number of localizations per trace by a factor of 100, as demonstrated with diffusing 30S ribosomal subunits in living Escherichia coli As conceptual limits have not been reached, we expect this localization modality to break new ground for observing the dynamics, distribution, and structure of macromolecules in living cells and beyond.", "doi": "10.1126/science.aak9913", "pmid": "28008086", "labels": [], "xrefs": [{"db": "pii", "key": "science.aak9913"}], "notes": [], "created": "2018-12-05T08:47:03.990Z", "modified": "2026-09-22T19:58:39.941Z"}]}