{"entity": "researcher", "timestamp": "2026-08-22T09:13:07.115Z", "family": "S\u00f6derh\u00e4ll", "given": "Cilla", "initials": "C", "orcid": "0000-0002-8397-3080", "affiliations": ["Dept of Biosciences and Nutrition, Karolinska Institutet, Stockholm, Sweden.", "Astrid Lindgren Children's Hospital, Karolinska University Hospital, Stockholm, Sweden.", "Dept of Women's and Children's Health, Karolinska Institutet, Stockholm, Sweden.", "These authors contributed equally."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/8e8f600e345a433291e43231c9f4d243.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/8e8f600e345a433291e43231c9f4d243"}}, "publications": [{"entity": "publication", "iuid": "5317cb6b3f9f46678580cd3f195d6120", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/5317cb6b3f9f46678580cd3f195d6120.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/5317cb6b3f9f46678580cd3f195d6120"}}, "title": "High-resolution targeted bisulfite sequencing reveals blood cell type-specific DNA methylation patterns in IL13 and ORMDL3.", "authors": [{"family": "S\u00f6derh\u00e4ll", "given": "Cilla", "initials": "C", "orcid": "0000-0002-8397-3080", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8e8f600e345a433291e43231c9f4d243.json"}}, {"family": "Reinius", "given": "Lovisa E", "initials": "LE"}, {"family": "Salmenper\u00e4", "given": "Pertteli", "initials": "P"}, {"family": "Gentile", "given": "Massimiliano", "initials": "M"}, {"family": "Acevedo", "given": "Nathalie", "initials": "N"}, {"family": "Konradsen", "given": "Jon R", "initials": "JR"}, {"family": "Nordlund", "given": "Bj\u00f6rn", "initials": "B"}, {"family": "Hedlin", "given": "Gunilla", "initials": "G"}, {"family": "Scheynius", "given": "Annika", "initials": "A"}, {"family": "Myllykangas", "given": "Samuel", "initials": "S"}, {"family": "Kere", "given": "Juha", "initials": "J"}], "type": "journal article", "published": "2021-05-10", "journal": {"title": "Clin Epigenetics", "issn": "1868-7083", "volume": "13", "issue": "1", "pages": "106", "issn-l": "1868-7075"}, "abstract": "Methylation of DNA at CpG sites is an epigenetic modification and a potential modifier of disease risk, possibly mediating environmental effects. Currently, DNA methylation is commonly assessed using specific microarrays that sample methylation at a few % of all methylated sites.\n\nTo understand if significant information on methylation can be added by a more comprehensive analysis of methylation, we set up a quantitative method, bisulfite oligonucleotide-selective sequencing (Bs-OS-seq), and compared the data with microarray-derived methylation data. We assessed methylation at two asthma-associated genes, IL13 and ORMDL3, in blood samples collected from children with and without asthma and fractionated white blood cell types from healthy adult controls.\n\nOur results show that Bs-OS-seq can uncover vast amounts of methylation variation not detected by commonly used array methods. We found that high-density methylation information from even one gene can delineate the main white blood cell lineages.\n\nWe conclude that high-resolution methylation studies can yield clinically important information at selected specific loci missed by array-based methods, with potential implications for future studies of methylation-disease associations.", "doi": "10.1186/s13148-021-01093-7", "pmid": "33971943", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC8111952"}, {"db": "pii", "key": "10.1186/s13148-021-01093-7"}], "notes": [], "created": "2026-08-21T12:40:58.549Z", "modified": "2026-08-21T12:40:58.608Z"}, {"entity": "publication", "iuid": "93bf55aab8f0402098e52556fc43a32b", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/93bf55aab8f0402098e52556fc43a32b.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/93bf55aab8f0402098e52556fc43a32b"}}, "title": "Nasal upregulation of CST1 in dog-sensitised children with severe allergic airway disease.", "authors": [{"family": "K\u00e4ck", "given": "Ulrika", "initials": "U"}, {"family": "Einarsdottir", "given": "Elisabet", "initials": "E"}, {"family": "van Hage", "given": "Marianne", "initials": "M"}, {"family": "Asarnoj", "given": "Anna", "initials": "A"}, {"family": "James", "given": "Anna", "initials": "A"}, {"family": "Nopp", "given": "Anna", "initials": "A"}, {"family": "Krjut\u0161kov", "given": "Kaarel", "initials": "K"}, {"family": "Katayama", "given": "Shintaro", "initials": "S", "orcid": "0000-0001-7581-5157", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ac94aab19cbd48d4970404f0d2b2e2ac.json"}}, {"family": "Kere", "given": "Juha", "initials": "J"}, {"family": "Lilja", "given": "Gunnar", "initials": "G"}, {"family": "S\u00f6derh\u00e4ll", "given": "Cilla", "initials": "C", "orcid": "0000-0002-8397-3080", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8e8f600e345a433291e43231c9f4d243.json"}}, {"family": "Konradsen", "given": "Jon R", "initials": "JR"}], "type": "journal article", "published": "2021-04-00", "journal": {"title": "ERJ Open Res", "issn": "2312-0541", "volume": "7", "issue": "2", "issn-l": null}, "abstract": "The clinical presentation of children sensitised to dog dander varies from asymptomatic to severe allergic airway disease, but the genetic mechanisms underlying these differences are not clear. The objective of the present study was to investigate nasal transcriptomic profiles associated with dog dander sensitisation in school children and to reveal clinical symptoms related with these profiles.\n\nRNA was extracted from nasal epithelial cell brushings of children sensitised to dog dander and healthy controls. Blood sample analyses included IgE against dog dander, dog allergen molecules, other airborne and food allergens, basophil activation and white blood cell counts. Clinical history of asthma and rhinitis was recorded, and lung function was assessed (spirometry, methacholine provocation and exhaled nitric oxide fraction).\n\nThe most overexpressed gene in children sensitised to dog dander compared to healthy controls was CST1, coding for Cystatin 1. A cluster of these children with enhanced CST1 expression showed lower forced expiratory volume in 1 s, increased bronchial hyperreactivity, pronounced eosinophilia and higher basophil allergen threshold sensitivity compared with other children sensitised to dog dander. In addition, multi-sensitisation to lipocalins was more common in this group.\n\nOverexpression of CST1 is associated with more severe allergic airway disease in children sensitised to dog dander. CST1 is thus a possible biomarker of the severity of allergic airway disease and a possible therapeutic target for the future treatment of airborne allergy.", "doi": "10.1183/23120541.00917-2020", "pmid": "33898616", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC8053908"}, {"db": "pii", "key": "00917-2020"}], "notes": [], "created": "2026-08-21T12:33:43.463Z", "modified": "2026-08-21T12:33:43.567Z"}]}