{"entity": "researcher", "timestamp": "2026-08-20T21:38:58.348Z", "family": "Souza", "given": "Paulo C T", "initials": "PCT", "orcid": "0000-0003-0660-1301", "affiliations": ["Laboratoire de Biologie et Mod\u00e9lisation de la Cellule, CNRS, UMR 5239, Inserm, U1293, Universit\u00e9 Claude Bernard Lyon 1, Ecole Normale Sup\u00e9rieure de Lyon, Lyon, France.", "Centre Blaise Pascal de Simulation et de Mod\u00e9lisation Num\u00e9rique, Ecole Normale Sup\u00e9rieure de Lyon , Lyon, France.", "Molecular Microbiology and Structural Biochemistry, CNRS UMR 5086 and Universit\u00e9 Claude Bernard Lyon 1 , Lyon, France."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/8c424f06c2304ac89afed85d40e0a706.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/8c424f06c2304ac89afed85d40e0a706"}}, "publications": [{"entity": "publication", "iuid": "a31f9689bd3b42db8093b0be00b5a2ab", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/a31f9689bd3b42db8093b0be00b5a2ab.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/a31f9689bd3b42db8093b0be00b5a2ab"}}, "title": "Spc2 modulates substrate- and cleavage site-selection in the yeast signal peptidase complex.", "authors": [{"family": "Chung", "given": "Yeonji", "initials": "Y", "orcid": "0009-0000-9326-6008", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9664b267a5364f399a6f05be47bda85a.json"}}, {"family": "Yim", "given": "Chewon", "initials": "C", "orcid": "0000-0002-0200-8458", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e61668a1319a4d9eaf0f10d7823d42ba.json"}}, {"family": "Pereira", "given": "Gilberto P", "initials": "GP", "orcid": "0000-0002-6277-0006", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d70da5b204ee458dbb41c6b58bbe928f.json"}}, {"family": "Son", "given": "Sungjoon", "initials": "S", "orcid": "0009-0008-7613-2305", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/08db25ca40a94ed99490435f908b4078.json"}}, {"family": "Kj\u00f8lbye", "given": "Lisbeth R", "initials": "LR", "orcid": "0000-0003-2834-3411", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4a1ce30e22b04e3ba42222b9cff63116.json"}}, {"family": "Mazurkiewicz", "given": "Lauren E", "initials": "LE", "orcid": "0000-0002-8045-6803", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3bbb9a31648c4b77b3a7e4195f987321.json"}}, {"family": "Weeks", "given": "Amy M", "initials": "AM", "orcid": "0000-0003-4700-8256", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/cdcdd9f864094e76beae3c3e75e82d0d.json"}}, {"family": "F\u00f6rster", "given": "Friedrich", "initials": "F", "orcid": "0000-0002-6044-2746", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ccae3beba62145a7a16f8254920f92ac.json"}}, {"family": "von Heijne", "given": "Gunnar", "initials": "G", "orcid": "0000-0002-4490-8569", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/529a460e668a479ca7d9b9271375ef9f.json"}}, {"family": "Souza", "given": "Paulo C T", "initials": "PCT", "orcid": "0000-0003-0660-1301", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8c424f06c2304ac89afed85d40e0a706.json"}}, {"family": "Kim", "given": "Hyun", "initials": "H", "orcid": "0000-0003-2076-9020", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/77c6e34f86444f51b0642eb2967ee200.json"}}], "type": "journal article", "published": "2024-12-02", "journal": {"title": "J. Cell Biol.", "issn": "1540-8140", "volume": "223", "issue": "12", "issn-l": "0021-9525"}, "abstract": "Secretory proteins are critically dependent on the correct processing of their signal sequence by the signal peptidase complex (SPC). This step, which is essential for the proper folding and localization of proteins in eukaryotic cells, is still not fully understood. In eukaryotes, the SPC comprises four evolutionarily conserved membrane subunits (Spc1-3 and Sec11). Here, we investigated the role of Spc2, examining SPC cleavage efficiency on various models and natural signal sequences in yeast cells depleted of or with mutations in Spc2. Our data show that discrimination between substrates and identification of the cleavage site by SPC is compromised when Spc2 is absent or mutated. Molecular dynamics simulation of the yeast SPC AlphaFold2-Multimer model indicates that membrane thinning at the center of SPC is reduced without Spc2, suggesting a molecular explanation for the altered substrate recognition properties of SPC lacking Spc2. These results provide new insights into the molecular mechanisms by which SPC governs protein biogenesis.", "doi": "10.1083/jcb.202211035", "pmid": "39565596", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC11579918"}, {"db": "pii", "key": "277099"}], "notes": [], "created": "2026-08-20T09:38:02.527Z", "modified": "2026-08-20T09:38:03.016Z"}]}