{"entity": "researcher", "timestamp": "2026-08-20T20:46:40.207Z", "family": "Sabouri", "given": "Nasim", "initials": "N", "orcid": "0000-0002-4541-7702", "affiliations": ["Department of Medical Biochemistry and Biophysics, Science for Life Laboratory, Ume\u00e5 University, 90187 Ume\u00e5, Sweden."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/8bbda597fa314881a10de1f7a6ad09ee.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/8bbda597fa314881a10de1f7a6ad09ee"}}, "publications": [{"entity": "publication", "iuid": "3aece26036754a8f89bfecad5f024849", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/3aece26036754a8f89bfecad5f024849.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/3aece26036754a8f89bfecad5f024849"}}, "title": "Evidence for a functional interaction between yeast Pol \u03b5 and PCNA in vivo.", "authors": [{"family": "Singh", "given": "Noopur", "initials": "N"}, {"family": "Odai", "given": "Roni", "initials": "R", "orcid": "0009-0002-2280-3915", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/604a6d6e3cdb468d8c6acfb6a2e45ff9.json"}}, {"family": "Persson", "given": "Ulf", "initials": "U"}, {"family": "Bylund", "given": "G\u00f6ran O", "initials": "GO"}, {"family": "Obi", "given": "Ikenna", "initials": "I"}, {"family": "Sabouri", "given": "Nasim", "initials": "N", "orcid": "0000-0002-4541-7702", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8bbda597fa314881a10de1f7a6ad09ee.json"}}, {"family": "Atkinson", "given": "Gemma C", "initials": "GC", "orcid": "0000-0002-4861-4584", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f1879e0a722045769cfca0dd00d611a8.json"}}, {"family": "Johansson", "given": "Erik", "initials": "E", "orcid": "0000-0002-8526-6224", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/2e8695cbe08e41e0a9ee097e2f38a37b.json"}}], "type": "journal article", "published": "2025-11-26", "journal": {"title": "Nucleic Acids Res.", "issn": "1362-4962", "volume": "53", "issue": "22", "issn-l": "0305-1048"}, "abstract": "DNA replication relies on precise coordination between proteins, including the sliding clamp proliferating cell nuclear antigen (PCNA), which encircles DNA to interact with key players in replication and repair. While biochemical studies have demonstrated interactions between PCNA and DNA polymerases \u03b4 and \u03b5 during DNA synthesis, the functional role of the Pol \u03b5-PCNA interaction in vivo, particularly during leading strand synthesis, remains to be elucidated. To address this question, we employed AlphaFold to model how PCNA interact with four-subunit yeast Pol \u03b5. Our models revealed two distinct points of interaction between Pol \u03b5 and PCNA: one at the P-domain and another at a PIP-box, a classical PCNA interaction motif. To validate these findings, we generated mutants that disrupted the Pol \u03b5-PCNA interaction interface. Biochemical assays demonstrated that the PIP-box is critical for this interaction, with the P-domain serving as a secondary contact point. Notably, introducing these mutants into yeast, caused no phenotype in a wild-type background. However, when fewer origins are firing, resulting in longer stretches of leading strand synthesis before forks converge, strains expressing a Pol \u03b5 mutant lacking interaction with PCNA showed slower growth. These findings suggest that PCNA enhances the processivity of Pol \u03b5 both in vitro and in vivo.", "doi": "10.1093/nar/gkaf1339", "pmid": "41404803", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC12709181"}, {"db": "pii", "key": "8382373"}], "notes": [], "created": "2026-08-20T09:50:15.101Z", "modified": "2026-08-20T09:50:15.248Z"}, {"entity": "publication", "iuid": "b29be4239cbc4b56b42eb485d69d07ed", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/b29be4239cbc4b56b42eb485d69d07ed.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/b29be4239cbc4b56b42eb485d69d07ed"}}, "title": "Organelle-Specific Thiochromenocarbazole Imide Derivative as a Heavy-Atom-Free Type I Photosensitizer for Biomolecule-Triggered Image-Guided Photodynamic Therapy.", "authors": [{"family": "Saczuk", "given": "Karolina", "initials": "K"}, {"family": "Kassem", "given": "Ahmad", "initials": "A"}, {"family": "Dudek", "given": "Marta", "initials": "M", "orcid": "0000-0001-6749-0903", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8710cb4719a94c6c924b6f19f3d601c5.json"}}, {"family": "S\u00e1nchez", "given": "Dar\u00edo Puch\u00e1n", "initials": "DP", "orcid": "0009-0006-7176-080X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/fc962921b8bd4f3890c6d689a9a6fe8f.json"}}, {"family": "Khrouz", "given": "Lhoussain", "initials": "L"}, {"family": "Allain", "given": "Magali", "initials": "M"}, {"family": "Welch", "given": "Gregory C", "initials": "GC", "orcid": "0000-0002-3768-937X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/2af0133b2efa407b8cb90494557c18eb.json"}}, {"family": "Sabouri", "given": "Nasim", "initials": "N", "orcid": "0000-0002-4541-7702", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8bbda597fa314881a10de1f7a6ad09ee.json"}}, {"family": "Monnereau", "given": "Cyrille", "initials": "C", "orcid": "0000-0002-8928-2416", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e6bab902e3164fe4bc7510b8e671b46a.json"}}, {"family": "Josse", "given": "Pierre", "initials": "P"}, {"family": "Cabanetos", "given": "Cl\u00e9ment", "initials": "C", "orcid": "0000-0003-3781-887X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/dbc477ef93be42398c3182ec637639f4.json"}}, {"family": "Deiana", "given": "Marco", "initials": "M", "orcid": "0000-0002-7815-4494", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3bd27194cbcd411098f0a2c5341e0423.json"}}], "type": "journal article", "published": "2025-03-06", "journal": {"title": "J Phys Chem Lett", "issn": "1948-7185", "volume": "16", "issue": "9", "pages": "2273-2282", "issn-l": "1948-7185"}, "abstract": "Modern photodynamic therapy (PDT) demands next-generation photosensitizers (PSs) that overcome heavy-atom dependency and enhance efficacy beyond traditional, highly oxygen-dependent type II mechanisms. We introduce herein TCI-NH, as a thiochromenocarbazole imide derivative designed for type I photodynamic action. Upon light activation, TCI-NH efficiently favors superoxide (O2\u2022-) and PS-centered radical formation instead of singlet oxygen (1O2) generation. Its high luminescence efficiency and selective localization in both the endoplasmic reticulum and mitochondria enable precise, image-guided PDT. Notably, interactions with biomolecules, such as serum albumin or DNA, enhance TCI-NH's emission by up to 40-fold and amplify radical generation by up to 5-fold. With negligible dark toxicity, this results in \u223c120 nM photocytotoxicity along with an impressive phototherapeutic index exceeding 200. Real-time live-cell imaging revealed rapid, light-triggered cytotoxicity characterized by apoptotic body formation and extensive cellular damage. With its small size, heavy-atom-free structure, exceptional, organelle specificity, and therapeutic efficacy, TCI-NH sets a new benchmark for anticancer type I PDT.", "doi": "10.1021/acs.jpclett.5c00136", "pmid": "39988904", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC11891978"}], "notes": [], "created": "2026-08-20T08:11:09.584Z", "modified": "2026-08-20T08:11:09.927Z"}]}