{"entity": "researcher", "timestamp": "2026-08-20T20:25:29.395Z", "family": "Seashore-Ludlow", "given": "Brinton", "initials": "B", "orcid": "0000-0001-8658-5967", "affiliations": [], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/8a8ea4c3fdb14de1b651d0367b0a70a4.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/8a8ea4c3fdb14de1b651d0367b0a70a4"}}, "publications": [{"entity": "publication", "iuid": "32611c803cb04eae95d1ddae75137d90", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/32611c803cb04eae95d1ddae75137d90.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/32611c803cb04eae95d1ddae75137d90"}}, "title": "Interdisciplinary Study on Drug-Induced-Phospholipidosis of Repurposing Libraries through Machine Learning and Experimental Evaluation in Different Cell Lines", "authors": [{"family": "Wendt", "given": "Jens", "initials": "J", "orcid": "0009-0002-1826-7099", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e17e1dee52fe45dd888fe91f408ab8ec.json"}}, {"family": "Kuzikov", "given": "Maria", "initials": "M", "orcid": "0000-0001-8771-1865", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6f75fc6caafe4ebbb876387123b84608.json"}}, {"family": "Kalman", "given": "Adelinn", "initials": "A", "orcid": "0000-0002-1539-0633", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a1ee81b3c46f42129bce5600df72abf8.json"}}, {"family": "Reinshagen", "given": "Jeanette", "initials": "J", "orcid": "0000-0002-8080-9170", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/76a9ab71d84644c980ad0f1aee2565d3.json"}}, {"family": "Huchting", "given": "Johanna", "initials": "J", "orcid": "0000-0001-6368-3273", "researcher": {"href": 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"https://publications-affiliated.scilifelab.se/publication/303ca727bb294b8c9e22f801a127ea9e"}}, "title": "From Library to Landscape: Integrative Annotation Workflows for Compound Libraries in Drug Repurposing", "authors": [{"family": "Reinshagen", "given": "Jeanette", "initials": "J", "orcid": "0000-0002-8080-9170", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/76a9ab71d84644c980ad0f1aee2565d3.json"}}, {"family": "Seashore-Ludlow", "given": "Brinton", "initials": "B", "orcid": "0000-0001-8658-5967", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8a8ea4c3fdb14de1b651d0367b0a70a4.json"}}, {"family": "Gadiya", "given": "Yojana", "initials": "Y", "orcid": "0000-0002-7683-0452", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/870d6b76507b4b4ba3eab97417ef9509.json"}}, {"family": "Gustavsson", "given": "Anna Lena", "initials": "AL", "orcid": "0000-0003-4332-2336", "researcher": {"href": 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"Philip", "initials": "P", "orcid": "0000-0001-7655-2459", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0c3e96ec00194af0882b4c3188b34917.json"}}, {"family": "Zaliani", "given": "Andrea", "initials": "A", "orcid": "0000-0002-1740-8390", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/43d815becc3740cd813a0ec55374a385.json"}}, {"family": "Ballante", "given": "Flavio", "initials": "F", "orcid": "0000-0002-4831-3423", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8ce9aad230aa414886e3bf519937aad9.json"}}], "type": "posted-content", "published": "2025-07-29", "journal": {"issn-l": null}, "abstract": null, "doi": "10.1101/2025.07.23.666341", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T11:06:41.783Z", "modified": "2026-08-20T11:06:41.970Z"}, {"entity": "publication", "iuid": "9ad7b0746d304d4ba4fbebdfa50317b5", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/9ad7b0746d304d4ba4fbebdfa50317b5.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/9ad7b0746d304d4ba4fbebdfa50317b5"}}, "title": "Interdisciplinary Study on Drug-Induced-Phospholipidosis of Repurposing Libraries through Machine Learning and Experimental Evaluation in Different Cell Lines", "authors": [{"family": "Kuzikov", "given": "Maria", "initials": "M", "orcid": "0000-0001-8771-1865", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6f75fc6caafe4ebbb876387123b84608.json"}}, {"family": "Kalman", "given": "Adelinn", "initials": "A", "orcid": "0000-0002-1539-0633", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a1ee81b3c46f42129bce5600df72abf8.json"}}, {"family": "Reinshagen", "given": "Jeanette", "initials": "J", "orcid": "0000-0002-8080-9170", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/76a9ab71d84644c980ad0f1aee2565d3.json"}}, {"family": "Huchting", "given": "Johanna", "initials": "J", "orcid": "0000-0001-6368-3273", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0e5a24e61ac84a7f91160b32365a74f3.json"}}, {"family": "Qian", "given": "Kun", "initials": "K"}, {"family": "Axelsson", "given": "Hanna", "initials": "H", "orcid": "0000-0003-2365-1749", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/affc5ee12a2d47b6a7f9ab3df049991e.json"}}, {"family": "Oestling", "given": "Paeivi", "initials": "P", "orcid": "0000-0001-5501-466X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b7cc0571cd644d9aa64e1f9d89bc72f3.json"}}, {"family": "Seashore-Ludlow", "given": "Brinton", "initials": "B", "orcid": "0000-0001-8658-5967", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8a8ea4c3fdb14de1b651d0367b0a70a4.json"}}, {"family": "Gadiya", "given": "Yojana", "initials": "Y", "orcid": "0000-0002-7683-0452", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/870d6b76507b4b4ba3eab97417ef9509.json"}}, {"family": "Gribbon", "given": "Philip", "initials": "P", "orcid": "0000-0001-7655-2459", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0c3e96ec00194af0882b4c3188b34917.json"}}, {"family": "Zaliani", "given": "Andrea", "initials": "A", "orcid": "0000-0002-1740-8390", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/43d815becc3740cd813a0ec55374a385.json"}}], "type": "posted-content", "published": "2025-07-09", "journal": {"issn-l": null}, "abstract": null, "doi": "10.1101/2025.07.08.663638", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T11:06:28.230Z", "modified": "2026-08-20T11:06:28.549Z"}, {"entity": "publication", "iuid": "5786af48f5004c87b13db7dada38a822", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/5786af48f5004c87b13db7dada38a822.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/5786af48f5004c87b13db7dada38a822"}}, "title": "Evaluating feature extraction in ovarian cancer cell line co-cultures using deep neural networks.", "authors": [{"family": "Sharma", "given": "Osheen", "initials": "O", "orcid": "0009-0007-6079-0189", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4930af2825ba4aeea80086a02596f1d7.json"}}, {"family": "Gudoityte", "given": "Greta", "initials": "G"}, {"family": "Minozada", "given": "Rezan", "initials": "R"}, {"family": "Kallioniemi", "given": "Olli P", "initials": "OP"}, {"family": "Turkki", "given": "Riku", "initials": "R"}, {"family": "Paavolainen", "given": "Lassi", "initials": "L", "orcid": "0000-0003-1508-2718", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/cb150cb5204f4f20a6dadd2048f9d610.json"}}, {"family": "Seashore-Ludlow", "given": "Brinton", "initials": "B", "orcid": "0000-0001-8658-5967", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8a8ea4c3fdb14de1b651d0367b0a70a4.json"}}], "type": "journal article", "published": "2025-02-25", "journal": {"title": "Commun Biol", "issn": "2399-3642", "volume": "8", "issue": "1", "pages": "303", "issn-l": "2399-3642"}, "abstract": "Single-cell image analysis is crucial for studying drug effects on cellular morphology and phenotypic changes. Most studies focus on single cell types, overlooking the complexity of cellular interactions. Here, we establish an analysis pipeline to extract phenotypic features of cancer cells cultured with fibroblasts. Using high-content imaging, we analyze an oncology drug library across five cancer and fibroblast cell line co-culture combinations, generating 61,440 images and \u223c170 million single-cell objects. Traditional phenotyping with CellProfiler achieves an average enrichment score of 62.6% for mechanisms of action, while pre-trained neural networks (EfficientNetB0 and MobileNetV2) reach 61.0% and 62.0%, respectively. Variability in enrichment scores may reflect the use of multiple drug concentrations since not all induce significant morphological changes, as well as the cellular and genetic context of the treatment. Our study highlights nuanced drug-induced phenotypic variations and underscores the morphological heterogeneity of ovarian cancer cell lines and their response to complex co-culture environments.", "doi": "10.1038/s42003-025-07766-w", "pmid": "40000764", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC11862010"}, {"db": "pii", "key": "10.1038/s42003-025-07766-w"}], "notes": [], "created": "2026-08-20T09:25:24.815Z", "modified": "2026-08-20T09:25:24.973Z"}, {"entity": "publication", "iuid": "6f7d9b816dd7474189fcb25bc7abb2b5", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/6f7d9b816dd7474189fcb25bc7abb2b5.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/6f7d9b816dd7474189fcb25bc7abb2b5"}}, "title": "Minimising Source-Plate Swaps for Robotised Compound Dispensing in Microplates", "authors": [{"family": "Gindullin", "given": "Ramiz", "initials": "R", "orcid": "0000-0003-4947-9641", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/fe0f94d1b02a4ddebb68c4ee718992d7.json"}}, {"family": "Francisco Rodr\u00edguez", "given": "Mar\u00eda Andre\u00edna", "initials": "MA", "orcid": "0000-0001-8745-9858", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/2bd8dd95fcff4a36bb25ec06d2e05bb3.json"}}, {"family": "Seashore-Ludlow", "given": "Brinton", "initials": "B", "orcid": "0000-0001-8658-5967", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8a8ea4c3fdb14de1b651d0367b0a70a4.json"}}, {"family": "Spjuth", "given": "Ola", "initials": "O", "orcid": "0000-0002-8083-2864", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/2c192389f99d4801b91f3350e07dfb9e.json"}}], "type": "book-chapter", "published": "2025-00-00", "journal": {"issn": "0302-9743", "pages": "256-273", "issn-l": null}, "abstract": null, "doi": "10.1007/978-3-031-95973-8_16", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T06:37:49.600Z", "modified": "2026-08-20T06:37:49.796Z"}, {"entity": "publication", "iuid": "b14473323e934321b8443c64c0102100", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/b14473323e934321b8443c64c0102100.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/b14473323e934321b8443c64c0102100"}}, "title": "Delineating functional and molecular impact of ex vivo sample handling in precision medicine.", "authors": [{"family": "Struyf", "given": "Nona", "initials": "N", "orcid": "0000-0002-6975-0753", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/be1087023d40465ca8133ea9845cd968.json"}}, {"family": "\u00d6sterroos", "given": "Albin", "initials": "A", "orcid": "0000-0001-8749-7299", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/aafab8cd17e94d52a3cdeaf86d3acfa6.json"}}, {"family": "Vesterlund", "given": "Mattias", "initials": "M", "orcid": "0000-0001-9471-6592", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e443cdb01cc44a7fa786941b5c0b62d6.json"}}, {"family": "Arnroth", "given": "Cornelia", "initials": "C"}, {"family": "James", "given": "Tojo", "initials": "T"}, {"family": "Sunandar", "given": "Stephanie", "initials": "S"}, {"family": "Mermelekas", "given": "Georgios", "initials": "G"}, {"family": "Bohlin", "given": "Anna", "initials": "A"}, {"family": "Hamberg Levedahl", "given": "Kerstin", "initials": "K"}, {"family": "Bengtz\u00e9n", "given": "Sofia", "initials": "S"}, {"family": "Jafari", "given": "Rozbeh", "initials": "R", "orcid": "0000-0002-3396-4709", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/95546f158dde40709fbfbb822a8f89c5.json"}}, {"family": "Orre", "given": "Lukas M", "initials": "LM", "orcid": "0000-0002-0384-1003", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e290f7cb5dfd49eb93e63cb704459516.json"}}, {"family": "Lehti\u00f6", "given": "Janne", "initials": "J"}, {"family": "Lehmann", "given": "S\u00f6ren", "initials": "S"}, {"family": "\u00d6stling", "given": "P\u00e4ivi", "initials": "P"}, {"family": "Kallioniemi", "given": "Olli", "initials": "O"}, {"family": "Seashore-Ludlow", "given": "Brinton", "initials": "B", "orcid": "0000-0001-8658-5967", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8a8ea4c3fdb14de1b651d0367b0a70a4.json"}}, {"family": "Erkers", "given": "Tom", "initials": "T", "orcid": "0000-0002-2754-6287", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4a1539d1d6a944eb882fdb80eb450706.json"}}], "type": "journal article", "published": "2024-02-19", "journal": {"title": "NPJ Precis Oncol", "issn": "2397-768X", "volume": "8", "issue": "1", "pages": "38", "issn-l": null}, "abstract": "Consistent handling of samples is crucial for achieving reproducible molecular and functional testing results in translational research. Here, we used 229 acute myeloid leukemia (AML) patient samples to assess the impact of sample handling on high-throughput functional drug testing, mass spectrometry-based proteomics, and flow cytometry. Our data revealed novel and previously described changes in cell phenotype and drug response dependent on sample biobanking. Specifically, myeloid cells with a CD117 (c-KIT) positive phenotype decreased after biobanking, potentially distorting cell population representations and affecting drugs targeting these cells. Additionally, highly granular AML cell numbers decreased after freezing. Secondly, protein expression levels, as well as sensitivity to drugs targeting cell proliferation, metabolism, tyrosine kinases (e.g., JAK, KIT, FLT3), and BH3 mimetics were notably affected by biobanking. Moreover, drug response profiles of paired fresh and frozen samples showed that freezing samples can lead to systematic errors in drug sensitivity scores. While a high correlation between fresh and frozen for the entire drug library was observed, freezing cells had a considerable impact at an individual level, which could influence outcomes in translational studies. Our study highlights conditions where standardization is needed to improve reproducibility, and where validation of data generated from biobanked cohorts may be particularly important.", "doi": "10.1038/s41698-024-00528-7", "pmid": "38374206", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC10876937"}, {"db": "pii", "key": "10.1038/s41698-024-00528-7"}], "notes": [], "created": "2026-08-20T09:24:34.463Z", "modified": "2026-08-20T09:24:34.644Z"}, {"entity": "publication", "iuid": "5d4efd700268417eb78ab55bfcfa8197", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/5d4efd700268417eb78ab55bfcfa8197.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/5d4efd700268417eb78ab55bfcfa8197"}}, "title": "SMAC mimetics overcome apoptotic resistance in ovarian cancer through MSLN-TNF alpha axis", "authors": [{"family": "Coelho", "given": "Ricardo", "initials": "R", "orcid": "0000-0002-9340-3612", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ca76d4960649461a87cd5f1049fd8ce6.json"}}, {"family": "Seashore-Ludlow", "given": "Brinton", "initials": "B", "orcid": "0000-0001-8658-5967", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8a8ea4c3fdb14de1b651d0367b0a70a4.json"}}, {"family": "Sch\u00fctz", "given": "Sarah", "initials": "S", "orcid": "0009-0009-8679-3059", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e9e5de6ca3fc43d18a124c06bc140595.json"}}, {"family": "Christopher Lombardo", "given": "Flavio", "initials": "F", "orcid": "0000-0002-4853-6838", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3ba422ddea6d4394b61c1d8d8d0dcb5b.json"}}, {"family": "Moussaud-Lamodi\u00e8re", "given": "Elisabeth", "initials": "E"}, {"family": "Casanova", "given": "Ruben", "initials": "R"}, {"family": "Ficek-Pascual", "given": "Joanna", "initials": "J"}, {"family": "Brunhilde Labrosse", "given": "Kathrin", "initials": "K", "orcid": "0000-0002-7195-5360", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3c4143d2dff24ff5933a54813e39f9a5.json"}}, {"family": "Hensler", "given": "Michal", "initials": "M", "orcid": "0000-0002-8767-0722", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/457a29b54e624fb6a6fa7c8872ff161f.json"}}, {"family": "Lopez-Nunez", "given": "Monica", "initials": "M"}, {"family": "Rimmer", "given": "Natalie", "initials": "N"}, {"family": "Fedier", "given": "Andre", "initials": "A"}, {"family": "Lima", "given": "Renata", "initials": "R"}, {"family": "Montavon Sartorius", "given": "C\u00e9line", "initials": "C"}, {"family": "Kurzeder", "given": "Christian", "initials": "C"}, {"family": "Singer", "given": "Franziska", "initials": "F", "orcid": "0000-0002-6017-1595", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ebf0a2aa03924e05b11472836f677ada.json"}}, {"family": "Bertolini", "given": "Anne", "initials": "A", "orcid": "0000-0003-0691-0489", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e1ae2b6192854292a5c383ffec505bde.json"}}, {"family": "Fucikova", "given": "Jitka", "initials": "J"}, {"family": "R\u00e4tsch", "given": "Gunnar", "initials": "G"}, {"family": "Bodenmiller", "given": "Bernd", "initials": "B"}, {"family": "Kallioniemi", "given": "Olli", "initials": "O"}, {"family": "\u00d6stling", "given": "P\u00e4ivi", "initials": "P"}, {"family": "David", "given": "Leonor", "initials": "L", "orcid": "0000-0003-4207-9258", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5b19bb9cf8f342ea878c7978ceca3deb.json"}}, {"family": "Heinzelmann-Schwarz", "given": "Viola", "initials": "V", "orcid": "0000-0002-4056-3225", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4bdb89773372459687ca47bc21c977aa.json"}}, {"family": "Jacob", "given": "Francis", "initials": "F", "orcid": "0000-0002-0446-1942", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6929b72a79b34aa68c39613c746e16cd.json"}}], "type": "posted-content", "published": "2024-01-26", "journal": {"issn-l": null}, "abstract": null, "doi": "10.1101/2024.01.24.576987", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T10:51:50.737Z", "modified": "2026-08-20T10:51:51.177Z"}, {"entity": "publication", "iuid": "37e5b61d6d8b4f719da9978e5016e686", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/37e5b61d6d8b4f719da9978e5016e686.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/37e5b61d6d8b4f719da9978e5016e686"}}, "title": "The drug efficacy testing in 3D cultures platform identifies effective drugs for ovarian cancer patients.", "authors": [{"family": "\u00c5kerlund", "given": "Emma", "initials": "E"}, {"family": "Gudoityte", "given": "Greta", "initials": "G"}, {"family": "Moussaud-Lamodi\u00e8re", "given": "Elisabeth", "initials": "E", "orcid": "0000-0002-2359-6519", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5cac5b7c76714c1895a4e31d16616821.json"}}, {"family": "Lind", "given": "Olina", "initials": "O"}, {"family": "Bwanika", "given": "Henri Colyn", "initials": "HC", "orcid": "0000-0002-0834-4153", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/16d34ecedbe74a98831828b46d4e93e4.json"}}, {"family": "Lehti", "given": "Kaisa", "initials": "K", "orcid": "0000-0001-9110-8719", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/613d944b4c8c4942b871ba87f580e53e.json"}}, {"family": "Salehi", "given": "Sahar", "initials": "S"}, {"family": "Carlson", "given": "Joseph", "initials": "J"}, {"family": "Wallin", "given": "Emelie", "initials": "E"}, {"family": "Fernebro", "given": "Josefin", "initials": "J"}, {"family": "\u00d6stling", "given": "P\u00e4ivi", "initials": "P"}, {"family": "Kallioniemi", "given": "Olli", "initials": "O"}, {"family": "Joneborg", "given": "Ulrika", "initials": "U", "orcid": "0000-0002-1922-0738", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d70ae27229fc4723941b2c3ae238ba26.json"}}, {"family": "Seashore-Ludlow", "given": "Brinton", "initials": "B", "orcid": "0000-0001-8658-5967", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8a8ea4c3fdb14de1b651d0367b0a70a4.json"}}], "type": "journal article", "published": "2023-10-31", "journal": {"title": "NPJ Precis Oncol", "issn": "2397-768X", "volume": "7", "issue": "1", "pages": "111", "issn-l": null}, "abstract": "Most patients with advanced ovarian cancer (OC) relapse and progress despite systemic therapy, pointing to the need for improved and tailored therapy options. Functional precision medicine can help to identify effective therapies for individual patients in a clinically relevant timeframe. Here, we present a scalable functional precision medicine platform: DET3Ct (Drug Efficacy Testing in 3D Cultures), where the response of patient cells to drugs and drug combinations are quantified with live-cell imaging. We demonstrate the delivery of individual drug sensitivity profiles in 20 samples from 16 patients with ovarian cancer in both 2D and 3D culture formats, achieving over 90% success rate in providing results six days after operation. In this cohort all patients received carboplatin. The carboplatin sensitivity scores were significantly different for patients with a progression free interval (PFI) less than or equal to 12 months and those with more than 12 months (p < 0.05). We find that the 3D culture format better retains proliferation and characteristics of the in vivo setting. Using the DET3Ct platform we evaluate 27 tailored combinations with results available 10 days after operation. Notably, carboplatin and A-1331852 (Bcl-xL inhibitor) showed an additive effect in four of eight OC samples tested, while afatinib and A-1331852 led to synergy in five of seven OC models. In conclusion, our 3D DET3Ct platform can rapidly define potential, clinically relevant data on efficacy of existing drugs in OC for precision medicine purposes, as well as provide insights on emerging drugs and drug combinations that warrant testing in clinical trials.", "doi": "10.1038/s41698-023-00463-z", "pmid": "37907613", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC10618545"}, {"db": "pii", "key": "10.1038/s41698-023-00463-z"}], "notes": [], "created": "2026-08-20T09:24:32.105Z", "modified": "2026-08-20T09:24:32.314Z"}, {"entity": "publication", "iuid": "e18900613386411ca6ad306c75d7d436", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/e18900613386411ca6ad306c75d7d436.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/e18900613386411ca6ad306c75d7d436"}}, "title": "Delineating functional and molecular impact of ex vivo sample handling in precision medicine", "authors": [{"family": "Erkers", "given": "Tom", "initials": "T"}, {"family": "Struyf", "given": "Nona", "initials": "N"}, {"family": "Arnroth", "given": "Cornelia", "initials": "C"}, {"family": "Vesterlund", "given": "Mattias", "initials": "M", "orcid": "0000-0001-9471-6592", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e443cdb01cc44a7fa786941b5c0b62d6.json"}}, {"family": "James", "given": "Tojo", "initials": "T"}, {"family": "Sunandar", "given": "Stephanie", "initials": "S"}, {"family": "Bohlin", "given": "Anna", "initials": "A"}, {"family": "Hamberg-Levedahl", "given": "Kerstin", "initials": "K"}, {"family": "Bengtz\u00e9n", "given": "Sofia", "initials": "S"}, {"family": "Jafari", "given": "Rozbeh", "initials": "R", "orcid": "0000-0002-3396-4709", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/95546f158dde40709fbfbb822a8f89c5.json"}}, {"family": "Orre", "given": "Lukas", "initials": "L"}, {"family": "Lehti\u00f6", "given": "Janne", "initials": "J"}, {"family": "Lehmann", "given": "S\u00f6ren", "initials": "S"}, {"family": "Ostling", "given": "Paivi", "initials": "P"}, {"family": "Kallioniemi", "given": "Olli", "initials": "O"}, {"family": "Seashore-Ludlow", "given": "Brinton", "initials": "B", "orcid": "0000-0001-8658-5967", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8a8ea4c3fdb14de1b651d0367b0a70a4.json"}}], "type": "posted-content", "published": "2023-07-26", "journal": {"issn-l": null}, "abstract": null, "doi": "10.21203/rs.3.rs-3072667/v1", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T12:59:12.192Z", "modified": "2026-08-20T12:59:12.224Z"}, {"entity": "publication", "iuid": "755a716868a348c889b0c1417472fc91", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/755a716868a348c889b0c1417472fc91.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/755a716868a348c889b0c1417472fc91"}}, "title": "The Drug Efficacy Testing in Ex Vivo 3D Cultures (DETECt) platform enables rapid identification of effective drugs and drug combinations for ovarian cancer patients", "authors": [{"family": "Seashore-Ludlow", "given": "Brinton", "initials": "B", "orcid": "0000-0001-8658-5967", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8a8ea4c3fdb14de1b651d0367b0a70a4.json"}}, {"family": "\u00c5kerlund", "given": "Emma", "initials": "E"}, {"family": "Gudoityte", "given": "Greta", "initials": "G"}, {"family": "Moussaud-Lamodi\u00e8re", "given": "Elisabeth", "initials": "E", "orcid": "0000-0002-2359-6519", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5cac5b7c76714c1895a4e31d16616821.json"}}, {"family": "Lind", "given": "Olina", "initials": "O"}, {"family": "Bwanika", "given": "Henri Colyn", "initials": "HC", "orcid": "0000-0002-0834-4153", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/16d34ecedbe74a98831828b46d4e93e4.json"}}, {"family": "Lehti", "given": "Kaisa", "initials": "K"}, {"family": "Saheli", "given": "Sahar", "initials": "S"}, {"family": "Carlson", "given": "Joseph", "initials": "J"}, {"family": "Wallin", "given": "Emelie", "initials": "E"}, {"family": "Fernebro", "given": "Josefin", "initials": "J"}, {"family": "Ostling", "given": "Paivi", "initials": "P"}, {"family": "Kallioniemi", "given": "Olli", "initials": "O"}, {"family": "Joneborg", "given": "Ulrika", "initials": "U", "orcid": "0000-0002-1922-0738", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d70ae27229fc4723941b2c3ae238ba26.json"}}], "type": "posted-content", "published": "2023-04-12", "journal": {"issn-l": null}, "abstract": null, "doi": "10.21203/rs.3.rs-2742592/v1", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T12:58:57.481Z", "modified": "2026-08-20T12:58:57.556Z"}, {"entity": "publication", "iuid": "167689b802a3421985f996418d6856b3", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/167689b802a3421985f996418d6856b3.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/167689b802a3421985f996418d6856b3"}}, "title": "A Portable, Negative-Pressure Actuated, Dynamically Tunable Microfluidic Droplet Generator.", "authors": [{"family": "Trossbach", "given": "Martin", "initials": "M", "orcid": "0000-0002-2081-3629", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f64881ca226f420dbaf484928bce6ea0.json"}}, {"family": "de Lucas Sanz", "given": "Marta", "initials": "M"}, {"family": "Seashore-Ludlow", "given": "Brinton", "initials": "B", "orcid": "0000-0001-8658-5967", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8a8ea4c3fdb14de1b651d0367b0a70a4.json"}}, {"family": "Joensson", "given": "Haakan N", "initials": "HN"}], "type": "journal article", "published": "2022-10-25", "journal": {"title": "Micromachines (Basel)", "issn": "2072-666X", "volume": "13", "issue": "11", "issn-l": "2072-666X"}, "abstract": "Droplet microfluidics utilize a monodisperse water-in-oil emulsion, with an expanding toolbox offering a wide variety of operations on a range of droplet sizes at high throughput. However, translation of these capabilities into applications for non-expert laboratories to fully harness the inherent potential of microscale manipulations is woefully trailing behind. One major obstacle is that droplet microfluidic setups often rely on custom fabricated devices, costly liquid actuators, and are not easily set up and operated by non-specialists. This impedes wider adoption of droplet technologies in, e.g., the life sciences. Here, we demonstrate an easy-to-use minimal droplet production setup with a small footprint, built exclusively from inexpensive commercially sourced parts, powered and controlled by a laptop. We characterize the components of the system and demonstrate production of droplets ranging in volume from 3 to 21 nL in a single microfluidic device. Furthermore, we describe the dynamic tuning of droplet composition. Finally, we demonstrate the production of droplet-templated cell spheroids from primary cells, where the mobility and simplicity of the setup enables its use within a biosafety cabinet. Taken together, we believe this minimal droplet setup is ideal to drive broad adoption of droplet microfluidics technology.", "doi": "10.3390/mi13111823", "pmid": "36363843", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC9697964"}, {"db": "pii", "key": "mi13111823"}], "notes": [], "created": "2026-08-20T13:42:25.213Z", "modified": "2026-08-20T13:42:25.317Z"}, {"entity": "publication", "iuid": "2ba5bdee961340588155b7eda4b30423", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/2ba5bdee961340588155b7eda4b30423.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/2ba5bdee961340588155b7eda4b30423"}}, "title": "High-throughput cell spheroid production and assembly analysis by microfluidics and deep learning", "authors": [{"family": "Trossbach", "given": "Martin", "initials": "M", "orcid": "0000-0002-2081-3629", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f64881ca226f420dbaf484928bce6ea0.json"}}, {"family": "\u00c5kerlund", "given": "Emma", "initials": "E", "orcid": "0000-0003-0235-1368", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/fbee89133e264b7c859a7e95d1308b4f.json"}}, {"family": "Langer", "given": "Krzysztof", "initials": "K"}, {"family": "Seashore-Ludlow", "given": "Brinton", "initials": "B", "orcid": "0000-0001-8658-5967", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8a8ea4c3fdb14de1b651d0367b0a70a4.json"}}, {"family": "Joensson", "given": "Haakan N", "initials": "HN", "orcid": "0000-0002-7195-2629", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8f8574e82592473486eae43de9a56b3d.json"}}], "type": "posted-content", "published": "2022-10-05", "journal": {"issn-l": null}, "abstract": null, "doi": "10.1101/2022.10.02.510497", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T10:41:10.914Z", "modified": "2026-08-20T10:41:11.092Z"}]}