{"entity": "researcher", "timestamp": "2026-08-20T21:01:31.765Z", "family": "Landegren", "given": "Ulf", "initials": "U", "orcid": "0000-0002-7820-1000", "affiliations": ["Department of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala University, Uppsala, Sweden. ulf.landegren@igp.uu.se."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/8749cdcda45a43d8bb2df04b0b211f36.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/8749cdcda45a43d8bb2df04b0b211f36"}}, "publications": [{"entity": "publication", "iuid": "78c76a7f70ac4e538725e7fd89e96a16", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/78c76a7f70ac4e538725e7fd89e96a16.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/78c76a7f70ac4e538725e7fd89e96a16"}}, "title": "Sensitive and Specific Analyses of Colorectal Cancer Recurrence through Multiplex superRCA Mutation Detection in Blood Plasma.", "authors": [{"family": "Sandberg", "given": "Emma", "initials": "E", "orcid": "0009-0003-5681-4417", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5f583a5882174325974058d4cb3b1a9e.json"}}, {"family": "Nunes", "given": "Lu\u00eds", "initials": "L", "orcid": "0000-0002-3391-1607", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/7aa31ccf1801456bb57744978bd605fd.json"}}, {"family": "Edqvist", "given": "Per-Henrik", "initials": "PH"}, {"family": "Mathot", "given": "Lucy", "initials": "L", "orcid": "0000-0002-2990-2038", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/458c07a1748d4ce0ac5cff3d6836f350.json"}}, {"family": "Chen", "given": "Lei", "initials": "L"}, {"family": "Edgren", "given": "Tomas", "initials": "T"}, {"family": "Al Nassralla", "given": "Shahed", "initials": "S"}, {"family": "Glimelius", "given": "Bengt", "initials": "B", "orcid": "0000-0002-5440-791X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0a4a4dfe9cc6478baaaae4672181691c.json"}}, {"family": "Landegren", "given": "Ulf", "initials": "U", "orcid": "0000-0002-7820-1000", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8749cdcda45a43d8bb2df04b0b211f36.json"}}, {"family": "Sj\u00f6blom", "given": "Tobias", "initials": "T", "orcid": "0000-0001-6668-4140", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/851b95a56b3244fb9bc7cec8294e7ae7.json"}}], "type": "journal article", "published": "2024-01-27", "journal": {"title": "Cancers (Basel)", "issn": "2072-6694", "volume": "16", "issue": "3", "issn-l": "2072-6694"}, "abstract": "Mutation analysis of circulating tumor DNA (ctDNA) has applications in monitoring of colorectal cancer (CRC) patients for recurrence. Considering the low tumor fraction of ctDNA in cell-free DNA (cfDNA) isolated from blood plasma, the sensitivity of the detection method is important. Here, plasma DNA collected at diagnosis and follow-up from 25 CRC patients was analyzed using a multiplex superRCA mutation detection assay. The assay was also performed on genomic DNA (gDNA) from tumor and normal tissue from 20 of these patients. The lower limit of detection for most sequence variants was in the range of 10-5, while when analyzing cfDNA from plasma with a typical input of 33 ng, the practical detection limit was ~10-4 or 0.01% mutant allele frequency (MAF). In 17 of 19 patients with identified hotspot mutations in tumor gDNA, at least one hotspot mutation could be detected in plasma DNA at the time of diagnosis. The MAF increased at subsequent time points in four of the patients who experienced a clinical relapse. Multiplex superRCA analysis of the remaining six patients did not reveal any hotspot mutations. In conclusion, multiplex superRCA assays proved suitable for monitoring CRC patients by analyzing hotspot mutations in cfDNA, and dynamic changes in MAF were observed in patients with clinical relapse.", "doi": "10.3390/cancers16030549", "pmid": "38339300", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC10854605"}, {"db": "pii", "key": "cancers16030549"}], "notes": [], "created": "2026-08-20T13:40:00.649Z", "modified": "2026-08-20T13:40:00.755Z"}, {"entity": "publication", "iuid": "bd5589a02cc24d7ebdfd569f383e9442", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/bd5589a02cc24d7ebdfd569f383e9442.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/bd5589a02cc24d7ebdfd569f383e9442"}}, "title": "Sensitive and Specific Analyses of Colorectal Cancer Recurrence through Multiplex superRCA Mutation Detection in Blood Plasma", "authors": [{"family": "Sandberg", "given": "Emma", "initials": "E", "orcid": "0009-0003-5681-4417", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5f583a5882174325974058d4cb3b1a9e.json"}}, {"family": "Nunes", "given": "Lu\u00eds", "initials": "L", "orcid": "0000-0002-3391-1607", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/7aa31ccf1801456bb57744978bd605fd.json"}}, {"family": "Edqvist", "given": "Per Henrik", "initials": "PH"}, {"family": "Mathot", "given": "Lucy", "initials": "L"}, {"family": "Chen", "given": "Lei", "initials": "L"}, {"family": "Edgren", "given": "Tomas", "initials": "T"}, {"family": "Al Nassralla", "given": "Shahed", "initials": "S"}, {"family": "Glimelius", "given": "Bengt", "initials": "B", "orcid": "0000-0002-5440-791X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0a4a4dfe9cc6478baaaae4672181691c.json"}}, {"family": "Landegren", "given": "Ulf", "initials": "U", "orcid": "0000-0002-7820-1000", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8749cdcda45a43d8bb2df04b0b211f36.json"}}, {"family": "Sj\u00f6blom", "given": "Tobias", "initials": "T", "orcid": "0000-0001-6668-4140", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/851b95a56b3244fb9bc7cec8294e7ae7.json"}}], "type": "posted-content", "published": "2024-01-11", "journal": {"issn-l": null}, "abstract": null, "doi": "10.20944/preprints202401.0950.v1", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T12:56:38.266Z", "modified": "2026-08-20T12:56:38.348Z"}, {"entity": "publication", "iuid": "304521984beb455dbb21d9bfe9b081b7", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/304521984beb455dbb21d9bfe9b081b7.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/304521984beb455dbb21d9bfe9b081b7"}}, "title": "Surface protein profiling of prostate-derived extracellular vesicles by mass spectrometry and proximity assays.", "authors": [{"family": "Manouchehri Doulabi", "given": "Ehsan", "initials": "E", "orcid": "0000-0003-1639-2267", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ad12164c3f004575b93f26cf83df40eb.json"}}, {"family": "Fredolini", "given": "Claudia", "initials": "C"}, {"family": "Gallini", "given": "Radiosa", "initials": "R", "orcid": "0000-0002-2709-3337", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9f5efcff1f4d469e987921b3ba4ecefd.json"}}, {"family": "L\u00f6f", "given": "Liza", "initials": "L"}, {"family": "Shen", "given": "Qiujin", "initials": "Q"}, {"family": "Ikebuchi", "given": "Ryoyo", "initials": "R"}, {"family": "Dubois", "given": "Louise", "initials": "L"}, {"family": "Azimi", "given": "Alireza", "initials": "A"}, {"family": "Loudig", "given": "Olivier", "initials": "O"}, {"family": "Gabrielsson", "given": "Susanne", "initials": "S", "orcid": "0000-0003-1771-1346", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4813fa3ad1ba45e28376583c64093a47.json"}}, {"family": "Landegren", "given": "Ulf", "initials": "U", "orcid": "0000-0002-7820-1000", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8749cdcda45a43d8bb2df04b0b211f36.json"}}, {"family": "Larsson", "given": "Anders", "initials": "A", "orcid": "0000-0003-3161-0402", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5b44e49c49f644cfa3348bcc4bb0080f.json"}}, {"family": "Bergquist", "given": "Jonas", "initials": "J", "orcid": "0000-0002-4597-041X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a27dc0277ccf4b94960dcbdc0e655e30.json"}}, {"family": "Kamali-Moghaddam", "given": "Masood", "initials": "M", "orcid": "0000-0002-1303-2218", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/70fceb2370d74e39a8c26d17963981cc.json"}}], "type": "journal article", "published": "2022-12-22", "journal": {"title": "Commun Biol", "issn": "2399-3642", "volume": "5", "issue": "1", "pages": "1402", "issn-l": "2399-3642"}, "abstract": "Extracellular vesicles (EVs) are mediators of intercellular communication and a promising class of biomarkers. Surface proteins of EVs play decisive roles in establishing a connection with recipient cells, and they are putative targets for diagnostic assays. Analysis of the surface proteins can thus both illuminate the biological functions of EVs and help identify potential biomarkers. We developed a strategy combining high-resolution mass spectrometry (HRMS) and proximity ligation assays (PLA) to first identify and then validate surface proteins discovered on EVs. We applied our workflow to investigate surface proteins of small EVs found in seminal fluid (SF-sEV). We identified 1,014 surface proteins and verified the presence of a subset of these on the surface of SF-sEVs. Our work demonstrates a general strategy for deep analysis of EVs' surface proteins across patients and pathological conditions, proceeding from unbiased screening by HRMS to ultra-sensitive targeted analyses via PLA.", "doi": "10.1038/s42003-022-04349-x", "pmid": "36550367", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC9780212"}, {"db": "pii", "key": "10.1038/s42003-022-04349-x"}], "notes": [], "created": "2026-08-20T09:25:13.924Z", "modified": "2026-08-20T09:25:14.135Z"}, {"entity": "publication", "iuid": "838c770f30814f348bb9ba386d67f89f", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/838c770f30814f348bb9ba386d67f89f.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/838c770f30814f348bb9ba386d67f89f"}}, "title": "Magnetic Bead Based Proximity Extension Assay for Sensitive Protein and Extracellular Vesicles Detection", "authors": [{"family": "Muthelo", "given": "Phathutshedzo", "initials": "P"}, {"family": "Ebai", "given": "Tonge", "initials": "T"}, {"family": "Doulabi", "given": "Ehsan Manouchehri", "initials": "EM"}, {"family": "Al-Amin", "given": "Rasel A", "initials": "RA", "orcid": "0000-0002-0762-9034", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/cb1973f718214a19a5dc787b443566df.json"}}, {"family": "Wu", "given": "Yajun", "initials": "Y"}, {"family": "Kamali-Moghaddam", "given": "Masood", "initials": "M"}, {"family": "Landegren", "given": "Ulf", "initials": "U", "orcid": "0000-0002-7820-1000", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8749cdcda45a43d8bb2df04b0b211f36.json"}}], "type": "posted-content", "published": "2022-11-27", "journal": {"issn-l": null}, "abstract": null, "doi": "10.1101/2022.11.27.518072", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T10:42:29.644Z", "modified": "2026-08-20T10:42:29.716Z"}, {"entity": "publication", "iuid": "c0b18f64d2134a9880f34da87b2ebef8", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/c0b18f64d2134a9880f34da87b2ebef8.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/c0b18f64d2134a9880f34da87b2ebef8"}}, "title": "Monitoring Drug-Target Interactions through Target Engagement-Mediated Amplification on Arrays and in situ", "authors": [{"family": "Al-Amin", "given": "Rasel", "initials": "R", "orcid": "0000-0002-0762-9034", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/cb1973f718214a19a5dc787b443566df.json"}}, {"family": "Johansson", "given": "Lars", "initials": "L"}, {"family": "Abdurakhmanov", "given": "Eldar", "initials": "E"}, {"family": "Landegren", "given": "Nils", "initials": "N"}, {"family": "L\u00f6f", "given": "Liza", "initials": "L"}, {"family": "Arng\u00e5rden", "given": "Linda", "initials": "L"}, {"family": "Blokzijl", "given": "Andries", "initials": "A"}, {"family": "Svensson", "given": "Richard", "initials": "R"}, {"family": "Hammond", "given": "Maria", "initials": "M"}, {"family": "L\u00f6nn", "given": "Peter", "initials": "P", "orcid": "0000-0002-2405-9010", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/7fad118bf4d946889aac3349868656fa.json"}}, {"family": "Haybaeck", "given": "Johannes", "initials": "J"}, {"family": "Kamali-Moghaddam", "given": "Masood", "initials": "M", "orcid": "0000-0002-1303-2218", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/70fceb2370d74e39a8c26d17963981cc.json"}}, {"family": "Jensen", "given": "Annika", "initials": "A"}, {"family": "Danielson", "given": "U Helena", "initials": "UH", "orcid": "0000-0003-2728-0340", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c1be15d9a1ea49e1b0e7bb712522696e.json"}}, {"family": "Artursson", "given": "Per", "initials": "P", "orcid": "0000-0002-3708-7395", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9908209a413a4088b6bf44a924d27319.json"}}, {"family": "Lundb\u00e4ck", "given": "Thomas", "initials": "T"}, {"family": "Landegren", "given": "Ulf", "initials": "U", "orcid": "0000-0002-7820-1000", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8749cdcda45a43d8bb2df04b0b211f36.json"}}], "type": "posted-content", "published": "2021-12-06", "journal": {"issn-l": null}, "abstract": null, "doi": "10.21203/rs.3.rs-1109577/v1", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T12:58:03.229Z", "modified": "2026-08-20T12:58:03.330Z"}, {"entity": "publication", "iuid": "ff6aff99d17f4c6385488d898ce4794a", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/ff6aff99d17f4c6385488d898ce4794a.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/ff6aff99d17f4c6385488d898ce4794a"}}, "title": "Image-based high-throughput mapping of TGF-\u03b2-induced phosphocomplexes at a single-cell level.", "authors": [{"family": "L\u00f6nn", "given": "Peter", "initials": "P", "orcid": "0000-0002-2405-9010", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/7fad118bf4d946889aac3349868656fa.json"}}, {"family": "Al-Amin", "given": "Rasel A", "initials": "RA", "orcid": "0000-0002-0762-9034", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/cb1973f718214a19a5dc787b443566df.json"}}, {"family": "Doulabi", "given": "Ehsan Manouchehri", "initials": "EM", "orcid": "0000-0003-1639-2267", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ad12164c3f004575b93f26cf83df40eb.json"}}, {"family": "Heldin", "given": "Johan", "initials": "J", "orcid": "0000-0002-0915-5303", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0c54a4210f844ab193a951c020d7406b.json"}}, {"family": "Gallini", "given": "Radiosa", "initials": "R"}, {"family": "Bj\u00f6rkesten", "given": "Johan", "initials": "J"}, {"family": "Oelrich", "given": "Johan", "initials": "J"}, {"family": "Kamali-Moghaddam", "given": "Masood", "initials": "M", "orcid": "0000-0002-1303-2218", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/70fceb2370d74e39a8c26d17963981cc.json"}}, {"family": "Landegren", "given": "Ulf", "initials": "U", "orcid": "0000-0002-7820-1000", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8749cdcda45a43d8bb2df04b0b211f36.json"}}], "type": "journal article", "published": "2021-11-12", "journal": {"title": "Commun Biol", "issn": "2399-3642", "volume": "4", "issue": "1", "pages": "1284", "issn-l": "2399-3642"}, "abstract": "Protein interactions and posttranslational modifications orchestrate cellular responses to e.g. cytokines and drugs, but it has been difficult to monitor these dynamic events in high-throughput. Here, we describe a semi-automated system for large-scale in situ proximity ligation assays (isPLA), combining isPLA in microtiter wells with automated microscopy and computer-based image analysis. Phosphorylations and interactions are digitally recorded along with subcellular morphological features. We investigated TGF-\u03b2-responsive Smad2 linker phosphorylations and complex formations over time and across millions of individual cells, and we relate these events to cell cycle progression and local cell crowding via measurements of DNA content and nuclear size of individual cells, and of their relative positions. We illustrate the suitability of this protocol to screen for drug effects using phosphatase inhibitors. Our approach expands the scope for image-based single cell analyses by combining observations of protein interactions and modifications with morphological details of individual cells at high throughput.", "doi": "10.1038/s42003-021-02798-4", "pmid": "34773084", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC8590043"}, {"db": "pii", "key": "10.1038/s42003-021-02798-4"}], "notes": [], "created": "2026-08-20T09:25:02.715Z", "modified": "2026-08-20T09:25:02.991Z"}, {"entity": "publication", "iuid": "72e57d53b80347cd92a63ae3a5358c01", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/72e57d53b80347cd92a63ae3a5358c01.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/72e57d53b80347cd92a63ae3a5358c01"}}, "title": "A myopic perspective on the future of protein diagnostics", "authors": [{"family": "Landegren", "given": "Ulf", "initials": "U", "orcid": "0000-0002-7820-1000", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8749cdcda45a43d8bb2df04b0b211f36.json"}}, {"family": "Al-Amin", "given": "Rasel A", "initials": "RA"}, {"family": "Bj\u00f6rkesten", "given": "Johan", "initials": "J"}], "type": "posted-content", "published": "2017-12-04", "journal": {"issn-l": null}, "abstract": null, "doi": "10.1101/227728", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T11:07:55.644Z", "modified": "2026-08-20T11:07:55.695Z"}]}