{"entity": "researcher", "timestamp": "2026-03-17T00:38:21.275Z", "family": "Eskafinoghani", "given": "Amirhesam", "initials": "A", "orcid": "0000-0002-9717-4810", "affiliations": [], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/870acfdb0dcb4f978107a194d485df9f.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/870acfdb0dcb4f978107a194d485df9f"}}, "publications": [{"entity": "publication", "iuid": "97db9245561b4895816a8a5c19b7ce9d", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/97db9245561b4895816a8a5c19b7ce9d.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/97db9245561b4895816a8a5c19b7ce9d"}}, "title": "Early gonadotoxic effects of cyclophosphamide on the prepubertal testis and the feasibility of reducing toxicity through combined antioxidant therapy.", "authors": [{"family": "Eskafinoghani", "given": "Amirhesam", "initials": "A", "orcid": "0000-0002-9717-4810", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/870acfdb0dcb4f978107a194d485df9f.json"}}, {"family": "Palomares", "given": "Arturo Reyes", "initials": "AR"}, {"family": "Hao", "given": "Xia", "initials": "X"}, {"family": "Mohammadi", "given": "Roudabeh", "initials": "R"}, {"family": "Lundberg", "given": "Arian", "initials": "A", "orcid": "0000-0002-6630-2787", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/baa2c7c8963840bfb6a22d7c5cfe35f9.json"}}, {"family": "Rodriguez-Walberg", "given": "Kenny A", "initials": "KA", "orcid": "0000-0003-4378-6181", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ab738f3602664a20bb1db36e55156d8b.json"}}], "type": "journal article", "published": "2025-12-29", "journal": {"title": "Reprod. Toxicol.", "issn": "1873-1708", "pages": "109156", "volume": "140", "issn-l": "0890-6238"}, "abstract": "Early chemotherapy-induced gonad toxicity threatens future fertility in boys, yet early in-vivo testicular responses are poorly defined. To characterize acute effects of cyclophosphamide (CPA) on the prepubertal testis and explore whether combined antioxidants (AO; L-carnitine [LC] and N-acetyl cysteine [NAC]) modulate these changes. CBA/B6 F1 male pups (postnatal day 7-9) were randomized to saline control, CPA (100 mg/kg i.p.), AO, or CPA+AO. Testes were collected every 8 h to 48 h for histology/immunostaining and were pooled (n = 3 per group/time point) for bulk RNA-seq per group/time point. Histology showed emerging degeneration from \u223c32 h with prominent effects by 48 h after CPA, including reduced germ cell layers, increased \u03b3H2AX/CC3, and decreased Ki67. Transcriptionally, CPA perturbed apoptosis/developmental pathways as early as 16 h, preceding overt histological change. AO and CPA+AO groups displayed partial transcriptional shifts toward control profiles, consistent with mitigation of CPA-associated signatures, but not full normalization. In neonatal mouse testis, CPA elicits rapid transcriptomic reprogramming within 16 h, before morphological injury at \u223c32-48 h. Concomitant AO shows preliminary, partial protective transcriptional effects. These proof-of-concept data support transcriptomics as an early, sensitive readout of testicular toxicity and motivate follow-up studies with independent validation and long-term outcomes prior to clinical translation.", "doi": "10.1016/j.reprotox.2025.109156", "pmid": "41475676", "labels": {"DDLS Fellow": null, "Arian Lundberg": null}, "xrefs": [{"db": "pii", "key": "S0890-6238(25)00327-2"}], "notes": [], "created": "2026-01-03T12:14:31.089Z", "modified": "2026-01-16T07:28:12.095Z"}]}