{"entity": "researcher", "timestamp": "2026-09-23T16:29:23.915Z", "family": "Astorga-Wells", "given": "Juan", "initials": "J", "orcid": "0000-0003-1017-8841", "affiliations": ["Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE-17177 Stockholm, Sweden.", "SciLifeLab, SE-17121 Solna, Sweden."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/855ef6e5db6f4791a52eeae9ffb95a30.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/855ef6e5db6f4791a52eeae9ffb95a30"}}, "publications": [{"entity": "publication", "iuid": "99f62b09e0c146edba8556aaa8b73440", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/99f62b09e0c146edba8556aaa8b73440.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/99f62b09e0c146edba8556aaa8b73440"}}, "title": "A bispecific CD40 agonistic antibody allowing for antibody-peptide conjugate formation to enable cancer-specific peptide delivery, resulting in improved T proliferation and anti-tumor immunity in mice.", "authors": [{"family": "Mebrahtu", "given": "Aman", "initials": "A"}, {"family": "Laur\u00e9n", "given": "Ida", "initials": "I", "orcid": "0000-0003-0041-6084", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6188000b91e54759af7c6789b7c4c63e.json"}}, {"family": "Veerman", "given": "Rosanne", "initials": "R"}, {"family": "Akpinar", "given": "G\u00f6zde G\u00fccl\u00fcler", "initials": "GG"}, {"family": "Lord", "given": "Martin", "initials": "M", "orcid": "0000-0002-3238-3187", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9049f3f7eab747bf8bf4749e71cd0873.json"}}, {"family": "Kostakis", "given": "Alexandros", "initials": "A", "orcid": "0009-0006-2252-2645", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3c3a0fe0badd4eac9fafc9497280e613.json"}}, {"family": "Astorga-Wells", "given": "Juan", "initials": "J", "orcid": "0000-0003-1017-8841", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/855ef6e5db6f4791a52eeae9ffb95a30.json"}}, {"family": "Dahllund", "given": "Leif", "initials": "L"}, {"family": "Olsson", "given": "Anders", "initials": "A"}, {"family": "Andersson", "given": "Oscar", "initials": "O"}, {"family": "Persson", "given": "Jonathan", "initials": "J"}, {"family": "Persson", "given": "Helena", "initials": "H"}, {"family": "D\u00f6nnes", "given": "Pierre", "initials": "P", "orcid": "0000-0002-4613-2952", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/307c54a242e24f77b06695e8b7e99ee8.json"}}, {"family": "Rockberg", "given": "Johan", "initials": "J"}, {"family": "Mangsbo", "given": "Sara", "initials": "S", "orcid": "0000-0002-1355-2678", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f4300802cdbd42b9843fe62bc6c96c33.json"}}], "type": "journal article", "published": "2024-11-05", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "15", "issue": "1", "pages": "9542", "issn-l": "2041-1723"}, "abstract": "Current antibody-based immunotherapy depends on tumor antigen shedding for proper T cell priming. Here we select a novel human CD40 agonistic drug candidate and generate a bispecific antibody, herein named BiA9*2_HF, that allows for rapid antibody-peptide conjugate formation. The format is designed to facilitate peptide antigen delivery to CD40 expressing cells combined with simultaneous CD40 agonistic activity. In vivo, the selected bispecific antibody BiA9*2_HF loaded with peptide cargos induces improved antigen-specific proliferation of CD8+ (10-15 fold) and CD4+ T cells (2-7 fold) over control in draining lymph nodes. In both virus-induced and neoantigen-based mouse tumor models, BiA9*2_HF demonstrates therapeutic efficacy and elevated safety profile, with complete tumor clearance, as well as measured abscopal impact on tumor growth. The BiA9*2_HF drug candidate can thus be utilized to tailor immunotherapeutics for cancer patients.", "doi": "10.1038/s41467-024-53839-5", "pmid": "39500897", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC11538452"}, {"db": "pii", "key": "10.1038/s41467-024-53839-5"}], "notes": [], "created": "2026-09-23T09:10:54.592Z", "modified": "2026-09-23T09:10:54.720Z"}, {"entity": "publication", "iuid": "c28fb876d1334187af97136f2fff26d0", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/c28fb876d1334187af97136f2fff26d0.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/c28fb876d1334187af97136f2fff26d0"}}, "title": "System-wide identification and prioritization of enzyme substrates by thermal analysis.", "authors": [{"family": "Saei", "given": "Amir Ata", "initials": "AA", "orcid": "0000-0002-2639-6328", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ebf703f0f2d44b14846ea1d9811b6e0d.json"}}, {"family": "Beusch", "given": "Christian M", "initials": "CM", "orcid": "0000-0001-9100-8283", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/fbb4771768354080ab5292c96fee0812.json"}}, {"family": "Sabatier", "given": "Pierre", "initials": "P", "orcid": "0000-0002-2734-1791", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c4cf83f2cf534d7eb308dab89d6460e2.json"}}, {"family": "Wells", "given": "Juan Astorga", "initials": "JA", "orcid": "0000-0003-1017-8841", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/855ef6e5db6f4791a52eeae9ffb95a30.json"}}, {"family": "Gharibi", "given": "Hassan", "initials": "H", "orcid": "0000-0002-3072-4929", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5825ec93851a42618425b67a5bbfbf3f.json"}}, {"family": "Meng", "given": "Zhaowei", "initials": "Z"}, {"family": "Chernobrovkin", "given": "Alexey", "initials": "A", "orcid": "0000-0001-7709-0161", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/011675da8d7a4fd68fbe5ce03227978d.json"}}, {"family": "Rodin", "given": "Sergey", "initials": "S"}, {"family": "N\u00e4reoja", "given": "Katja", "initials": "K"}, {"family": "Thorsell", "given": "Ann-Gerd", "initials": "AG"}, {"family": "Karlberg", "given": "Tobias", "initials": "T"}, {"family": "Cheng", "given": "Qing", "initials": "Q"}, {"family": "Lundstr\u00f6m", "given": "Susanna L", "initials": "SL"}, {"family": "Gaetani", "given": "Massimiliano", "initials": "M", "orcid": "0000-0001-5610-0797", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a402c63fbe4f478daa286177f031b923.json"}}, {"family": "V\u00e9gv\u00e1ri", "given": "\u00c1kos", "initials": "\u00c1", "orcid": "0000-0002-1287-0906", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/724d65ef088147c4988093b2b6b5f318.json"}}, {"family": "Arn\u00e9r", "given": "Elias S J", "initials": "ESJ", "orcid": "0000-0002-4807-6114", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3502128dcae54dfcb6808356694bf4dc.json"}}, {"family": "Sch\u00fcler", "given": "Herwig", "initials": "H", "orcid": "0000-0003-4059-3501", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6a66d04210ea41c2bbb0a5ee187c7b76.json"}}, {"family": "Zubarev", "given": "Roman A", "initials": "RA", "orcid": "0000-0001-9839-2089", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5e3f9910c1ff434c8056bdecf537e9ef.json"}}], "type": "journal article", "published": "2021-02-26", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "12", "issue": "1", "pages": "1296", "issn-l": "2041-1723"}, "abstract": "Despite the immense importance of enzyme-substrate reactions, there is a lack of general and unbiased tools for identifying and prioritizing substrate proteins that are modified by the enzyme on the structural level. Here we describe a high-throughput unbiased proteomics method called System-wide Identification and prioritization of Enzyme Substrates by Thermal Analysis (SIESTA). The approach assumes that the enzymatic post-translational modification of substrate proteins is likely to change their thermal stability. In our proof-of-concept studies, SIESTA successfully identifies several known and novel substrate candidates for selenoprotein thioredoxin reductase 1, protein kinase B (AKT1) and poly-(ADP-ribose) polymerase-10 systems. Wider application of SIESTA can enhance our understanding of the role of enzymes in homeostasis and disease, opening opportunities to investigate the effect of post-translational modifications on signal transduction and facilitate drug discovery.", "doi": "10.1038/s41467-021-21540-6", "pmid": "33637753", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC7910609"}, {"db": "pii", "key": "10.1038/s41467-021-21540-6"}], "notes": [], "created": "2026-09-23T08:34:01.574Z", "modified": "2026-09-23T08:34:01.919Z"}, {"entity": "publication", "iuid": "a23049ab7358499e961c8b95b86b483a", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/a23049ab7358499e961c8b95b86b483a.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/a23049ab7358499e961c8b95b86b483a"}}, "title": "Small-molecule inhibitor of OGG1 suppresses proinflammatory gene expression and inflammation.", "authors": [{"family": "Visnes", "given": "Torkild", "initials": "T", "orcid": "0000-0003-1047-988X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9fef10d6a8604971a6ad1849b9f8b029.json"}}, {"family": "C\u00e1zares-K\u00f6rner", "given": "Armando", "initials": "A", "orcid": "0000-0003-0765-3787", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8ba54907c24343e1b604095523ceea78.json"}}, {"family": "Hao", "given": "Wenjing", "initials": "W", "orcid": "0000-0002-8487-5968", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3a208bb26ff04b32a2903dbd2311a530.json"}}, {"family": "Wallner", "given": "Olov", "initials": "O", "orcid": "0000-0002-6481-237X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3601a5a4d88f4452b6d1ac5f22c79d9f.json"}}, {"family": "Masuyer", "given": "Geoffrey", "initials": "G", "orcid": "0000-0002-9527-2310", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c62ba219d3e34662aaa458dbd361cf31.json"}}, {"family": "Loseva", "given": "Olga", "initials": "O"}, {"family": "Mortusewicz", "given": "Oliver", "initials": "O"}, {"family": "Wiita", "given": "Elis\u00e9e", "initials": "E"}, {"family": "Sarno", "given": "Antonio", "initials": "A", "orcid": "0000-0002-9308-8018", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/22883e442f534ba99f2917cf45d95b3d.json"}}, {"family": "Manoilov", "given": "Aleksandr", "initials": "A"}, {"family": "Astorga-Wells", "given": "Juan", "initials": "J", "orcid": "0000-0003-1017-8841", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/855ef6e5db6f4791a52eeae9ffb95a30.json"}}, {"family": "Jemth", "given": "Ann-Sofie", "initials": "AS"}, {"family": "Pan", "given": "Lang", "initials": "L"}, {"family": "Sanjiv", "given": "Kumar", "initials": "K"}, {"family": "Karsten", "given": "Stella", "initials": "S", "orcid": "0000-0001-6191-4314", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3a27ec21e4a34600aa24a7213372e19c.json"}}, {"family": "Gokturk", "given": "Camilla", "initials": "C"}, {"family": "Grube", "given": "Maurice", "initials": "M"}, {"family": "Homan", "given": "Evert J", "initials": "EJ"}, {"family": "Hanna", "given": "Bishoy M F", "initials": "BMF", "orcid": "0000-0001-7027-6553", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/61301102836e44d189f5628bb33c1335.json"}}, {"family": "Paulin", "given": "Cynthia B J", "initials": "CBJ", "orcid": "0000-0002-5698-7962", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8290bdaf864c4f7980ce2b5637897653.json"}}, {"family": "Pham", "given": "Therese", "initials": "T"}, {"family": "Rasti", "given": "Azita", "initials": "A"}, {"family": "Berglund", "given": "Ulrika Warpman", "initials": "UW"}, {"family": "von Nicolai", "given": "Catharina", "initials": "C"}, {"family": "Benitez-Buelga", "given": "Carlos", "initials": "C"}, {"family": "Koolmeister", "given": "Tobias", "initials": "T"}, {"family": "Ivanic", "given": "Dag", "initials": "D", "orcid": "0000-0002-6052-2236", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/537e93ab027c4287bbe06df648ef5e74.json"}}, {"family": "Iliev", "given": "Petar", "initials": "P", "orcid": "0000-0002-3905-9814", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/88791a216afa4eee80e5b0dfdd851d6f.json"}}, {"family": "Scobie", "given": "Martin", "initials": "M", "orcid": "0000-0002-7073-8495", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/bdce0df415d54e9d84a4fcbb4d1350e5.json"}}, {"family": "Krokan", "given": "Hans E", "initials": "HE"}, {"family": "Baranczewski", "given": "Pawel", "initials": "P"}, {"family": "Artursson", "given": "Per", "initials": "P", "orcid": "0000-0002-3708-7395", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9908209a413a4088b6bf44a924d27319.json"}}, {"family": "Altun", "given": "Mikael", "initials": "M"}, {"family": "Jensen", "given": "Annika Jenmalm", "initials": "AJ"}, {"family": "Kalder\u00e9n", "given": "Christina", "initials": "C"}, {"family": "Ba", "given": "Xueqing", "initials": "X", "orcid": "0000-0002-1075-5496", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e75bc54b28c54cbc9b4a3c0856aefedf.json"}}, {"family": "Zubarev", "given": "Roman A", "initials": "RA", "orcid": "0000-0001-9839-2089", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5e3f9910c1ff434c8056bdecf537e9ef.json"}}, {"family": "Stenmark", "given": "P\u00e5l", "initials": "P", "orcid": "0000-0003-4777-3417", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9791bc0d7463417899d6953b5ca1bac3.json"}}, {"family": "Boldogh", "given": "Istvan", "initials": "I"}, {"family": "Helleday", "given": "Thomas", "initials": "T", "orcid": "0000-0002-7384-092X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/385586c159494955a9a72c3381e3ad47.json"}}], "type": "journal article", "published": "2018-11-16", "journal": {"title": "Science (New York, N.Y.)", "issn": "1095-9203", "volume": "362", "issue": "6416", "pages": "834-839", "issn-l": "0036-8075"}, "abstract": "The onset of inflammation is associated with reactive oxygen species and oxidative damage to macromolecules like 7,8-dihydro-8-oxoguanine (8-oxoG) in DNA. Because 8-oxoguanine DNA glycosylase 1 (OGG1) binds 8-oxoG and because Ogg1-deficient mice are resistant to acute and systemic inflammation, we hypothesized that OGG1 inhibition may represent a strategy for the prevention and treatment of inflammation. We developed TH5487, a selective active-site inhibitor of OGG1, which hampers OGG1 binding to and repair of 8-oxoG and which is well tolerated by mice. TH5487 prevents tumor necrosis factor-\u03b1-induced OGG1-DNA interactions at guanine-rich promoters of proinflammatory genes. This, in turn, decreases DNA occupancy of nuclear factor \u03baB and proinflammatory gene expression, resulting in decreased immune cell recruitment to mouse lungs. Thus, we present a proof of concept that targeting oxidative DNA repair can alleviate inflammatory conditions in vivo.", "doi": "10.1126/science.aar8048", "pmid": "30442810", "labels": [], "xrefs": [{"db": "mid", "key": "NIHMS1026297"}, {"db": "pmc", "key": "PMC6645780"}, {"db": "pii", "key": "362/6416/834"}], "notes": [], "created": "2026-09-23T06:41:55.642Z", "modified": "2026-09-23T06:41:55.991Z"}]}