{"entity": "researcher", "timestamp": "2026-08-22T06:58:25.468Z", "family": "Arruda", "given": "Lucas C M", "initials": "LCM", "orcid": "0000-0002-8573-9618", "affiliations": ["Department of Clinical Science, Intervention and Technology, Karolinska Institute, Stockholm, Sweden."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/84d25ce6da6a4b21a4f147c6192386cd.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/84d25ce6da6a4b21a4f147c6192386cd"}}, "publications": [{"entity": "publication", "iuid": "5c8a0b9d88f4418893d7e9be0824cefc", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/5c8a0b9d88f4418893d7e9be0824cefc.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/5c8a0b9d88f4418893d7e9be0824cefc"}}, "title": "SARS-CoV-2 (COVID-19)-specific T cell and B cell responses in convalescent rheumatoid arthritis: Monozygotic twins pair case observation.", "authors": [{"family": "Arruda", "given": "Lucas C M", "initials": "LCM", "orcid": "0000-0002-8573-9618", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/84d25ce6da6a4b21a4f147c6192386cd.json"}}, {"family": "Gaballa", "given": "Ahmed", "initials": "A"}, {"family": "Da Silva Rodrigues", "given": "Rui", "initials": "R", "orcid": "0000-0001-7620-872X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/2ede811078d34b62a29e07a448354dbf.json"}}, {"family": "Makower", "given": "Bartek", "initials": "B"}, {"family": "Uhlin", "given": "Michael", "initials": "M"}], "type": "journal article", "published": "2022-05-00", "journal": {"title": "Scand. J. Immunol.", "issn": "1365-3083", "volume": "95", "issue": "5", "pages": "e13151", "issn-l": "0300-9475"}, "abstract": "Rheumatoid arthritis (RA) patients present higher risk of SARS-CoV-2 infection (COVID-19), and proper management of the disease in this population requires a better understanding of how the immune system controls the virus. We analyzed the T cell and B cell phenotypes, and their repertoire in a pair of monozygotic twins with RA mismatched for COVID-19 infection. Twin- was not infected, while Twin+ was infected and effectively controlled the infection. We found no significant changes on the \u03b1\u03b2 T cell composition, while \u03b3\u03b4 T cells and B cells presented considerable expansion of memory population in Twin+ and robust T/B cell responses to several SARS-CoV-2 peptides. T cell receptor \u03b2/\u03b3-chain and immunoglobulin heavy chain next-generation sequencing depicted a remarkable higher diversity in Twin+ compared with Twin-, despite no significant changes being found in variable/joining family usage. Repertoire overlap analyses showed that, although being identical twins, very few clones were shared between them, indicating that COVID-19 may lead to deep changes on the immune cell repertoire in RA patients. Altogether, our results indicate that RA patients may develop robust and persistent COVID-19-specific T/B cell responses; \u03b3\u03b4 T cells and B cells may play a key role in the management of COVID-19 in RA, and the infection may lead to a profound reshaping of immune cell receptor specificities.", "doi": "10.1111/sji.13151", "pmid": "35212005", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC9115348"}], "notes": [], "created": "2026-08-21T12:21:41.863Z", "modified": "2026-08-21T12:21:41.977Z"}, {"entity": "publication", "iuid": "38ab3ef241bf442d9c014e3e64758ef3", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/38ab3ef241bf442d9c014e3e64758ef3.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/38ab3ef241bf442d9c014e3e64758ef3"}}, "title": "CD8+ \u03b3\u03b4 T Cells Are More Frequent in CMV Seropositive Bone Marrow Grafts and Display Phenotype of an Adaptive Immune Response.", "authors": [{"family": "Gaballa", "given": "Ahmed", "initials": "A", "orcid": "0000-0002-5277-5129", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b062c2e7638440919938a93892cf7848.json"}}, {"family": "Arruda", "given": "Lucas C M", "initials": "LCM", "orcid": "0000-0002-8573-9618", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/84d25ce6da6a4b21a4f147c6192386cd.json"}}, {"family": "R\u00e5destad", "given": "Emelie", "initials": "E", "orcid": "0000-0002-4011-7401", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6bbfa04b4fad4b3ea31a4a193e2e7c2d.json"}}, {"family": "Uhlin", "given": "Michael", "initials": "M", "orcid": "0000-0002-2177-6727", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/116cfe482bb941159ee2e7175f1cd095.json"}}], "type": "journal article", "published": "2019-12-06", "journal": {"title": "Stem Cells Int", "issn": "1687-966X", "volume": "2019", "pages": "6348060", "issn-l": null}, "abstract": "The role of gamma delta (\u03b3\u03b4) T cells in human cytomegalovirus (HCMV) immune surveillance has been the focus of research interest for years. Recent reports have shown a substantial clonal proliferation of \u03b3\u03b4 T cells in response to HCMV, shedding light on the adaptive immune response of \u03b3\u03b4 T cells. Nevertheless, most efforts have focused on V\u03b42neg \u03b3\u03b4 T cell subset while less attention has been given to investigate other less common \u03b3\u03b4 T cell subsets. In this regard, a distinct subpopulation of \u03b3\u03b4 T cells that expresses the CD8 coreceptor (CD8+ \u03b3\u03b4 T cells) has not been thoroughly explored. Whether it is implicated in HCMV response and its ability to generate adaptive response has not been thoroughly investigated. In this study, we combined flow cytometry and immune sequencing of the TCR \u03b3-chain (TRG) to analyze in-depth bone marrow (BM) graft \u03b3\u03b4 T cells from CMV seropositive (CMV+) and CMV seronegative (CMV-) donors. We showed that the frequency of CD8+ \u03b3\u03b4 T cells was significantly higher in CMV+ grafts compared to CMV- grafts (P < 0.001). Further characterization revealed that CD8+ \u03b3\u03b4 T cells from CMV+ grafts express V\u03b39- and preferentially differentiated from a naive to terminal effector memory phenotype (CD27low/-CD45RO-). In line with these findings, TRG immune sequencing revealed clonal focusing and reduced usage of the V\u03b39/JP gene segment in a CMV+ graft. Furthermore, CD8+ \u03b3\u03b4 T cells showed an enhanced response to TCR/CD3 and cytokine stimulation in contrast to CD8- \u03b3\u03b4 T cells. We conclude that \u03b3\u03b4 T cells in BM grafts are reshaped by donor CMV serostatus and highlight the potential adaptive role of CD8+ \u03b3\u03b4 T cells in HCMV immune response.", "doi": "10.1155/2019/6348060", "pmid": "31885619", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC6925825"}], "notes": [], "created": "2026-08-21T12:28:26.405Z", "modified": "2026-08-21T12:28:26.565Z"}, {"entity": "publication", "iuid": "9aeba8acc1a848be80d9edceafbd406e", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/9aeba8acc1a848be80d9edceafbd406e.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/9aeba8acc1a848be80d9edceafbd406e"}}, "title": "Graft \u03b3\u03b4 TCR Sequencing Identifies Public Clonotypes Associated with Hematopoietic Stem Cell Transplantation Efficacy in Acute Myeloid Leukemia Patients and Unravels Cytomegalovirus Impact on Repertoire Distribution.", "authors": [{"family": "Arruda", "given": "Lucas C M", "initials": "LCM", "orcid": "0000-0002-8573-9618", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/84d25ce6da6a4b21a4f147c6192386cd.json"}}, {"family": "Gaballa", "given": "Ahmed", "initials": "A", "orcid": "0000-0002-5277-5129", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b062c2e7638440919938a93892cf7848.json"}}, {"family": "Uhlin", "given": "Michael", "initials": "M", "orcid": "0000-0002-2177-6727", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/116cfe482bb941159ee2e7175f1cd095.json"}}], "type": "journal article", "published": "2019-03-15", "journal": {"title": "J. Immunol.", "issn": "1550-6606", "volume": "202", "issue": "6", "pages": "1859-1870", "issn-l": "0022-1767"}, "abstract": "Although the impact of donor graft composition on clinical outcomes after hematopoietic stem cell transplantation (HSCT) has been studied, little is known about the role of intragraft \u03b3\u03b4 TCR repertoire on clinical outcomes following HSCT. Using a high-throughput sequencing platform, we sought to analyze the TCR \u03b3-chain (TRG) repertoire of \u03b3\u03b4 T cells within donor stem cell grafts and address its potential impact on clinical response in the corresponding patients. A total of 20 peripheral blood stem cell grafts were analyzed, and donors were classified as CMV+/- The respective acute myeloid leukemia recipients were followed for disease relapse and acute graft-versus-host disease (aGvHD) development post-HSCT. In all samples, TRG repertoire showed a reduced diversity and displayed overrepresented clones. This was more prominent in grafts from CMV+ donors, which presented a more private repertoire, lower diversity, skewed distribution, and reduced usage of the V9-JP pairing. Grafts given to nonrelapse patients presented a more public repertoire and increased presence of long sequence clonotypes. Variable-joining gene segment usage was not associated with aGvHD development, but a higher usage of V2-JP1 pairing and lower usage of V4-J2/V5-J2/V8-JP2 were observed in grafts given to nonrelapse patients. Our work identified five private overrepresented and one public CDR3 sequence (CATWDGPYYKKLF) associated with CMV infection, in addition to 12 highly frequent public sequences present exclusively in grafts given to nonrelapse patients. Our findings show that, despite CMV infection reshaping the TRG repertoire, TRG composition is not associated with aGvHD development, and several public sequences are associated with clinical remission.", "doi": "10.4049/jimmunol.1801448", "pmid": "30710048", "labels": [], "xrefs": [{"db": "pii", "key": "jimmunol.1801448"}], "notes": [], "created": "2026-08-21T13:06:12.037Z", "modified": "2026-08-21T13:06:12.081Z"}]}