{"entity": "researcher", "timestamp": "2026-08-20T21:08:36.304Z", "family": "Caidahl", "given": "Kenneth", "initials": "K", "orcid": "0000-0002-5079-9696", "affiliations": ["Department of Molecular Medicine and Surgery, Center for Molecular Medicine, Karolinska Institutet, 17176 Stockholm, Sweden.", "Department of Molecular and Clinical Medicine, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, 41345 Gothenburg, Sweden."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/848cce5f4b814e589d2e7fa26d98be72.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/848cce5f4b814e589d2e7fa26d98be72"}}, "publications": [{"entity": "publication", "iuid": "bda398a8713a4a8090b7784773e10ca4", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/bda398a8713a4a8090b7784773e10ca4.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/bda398a8713a4a8090b7784773e10ca4"}}, "title": "Molecular Imaging of a New Multimodal Microbubble for Adhesion Molecule Targeting.", "authors": [{"family": "Ahmed", "given": "Mona", "initials": "M"}, {"family": "Gustafsson", "given": "Bj\u00f6rn", "initials": "B"}, {"family": "Aldi", "given": "Silvia", "initials": "S"}, {"family": "Dusart", "given": "Philip", "initials": "P"}, {"family": "Egri", "given": "Gabriella", "initials": "G"}, {"family": "Butler", "given": "Lynn M", "initials": "LM"}, {"family": "Bone", "given": "Dianna", "initials": "D"}, {"family": "D\u00e4hne", "given": "Lars", "initials": "L"}, {"family": "Hedin", "given": "Ulf", "initials": "U"}, {"family": "Caidahl", "given": "Kenneth", "initials": "K", "orcid": "0000-0002-5079-9696", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/848cce5f4b814e589d2e7fa26d98be72.json"}}], "type": "journal article", "published": "2019-02-00", "journal": {"title": "Cell Mol Bioeng", "issn": "1865-5025", "volume": "12", "issue": "1", "pages": "15-32", "issn-l": null}, "abstract": "Inflammation is an important risk-associated component of many diseases and can be diagnosed by molecular imaging of specific molecules. The aim of this study was to evaluate the possibility of targeting adhesion molecules on inflammation-activated endothelial cells and macrophages using an innovative multimodal polyvinyl alcohol-based microbubble (MB) contrast agent developed for diagnostic use in ultrasound, magnetic resonance, and nuclear imaging.\n\nWe assessed the binding efficiency of antibody-conjugated multimodal contrast to inflamed murine or human endothelial cells (ECs), and to peritoneal macrophages isolated from rats with peritonitis, utilizing the fluorescence characteristics of the MBs. Single-photon emission tomography (SPECT) was used to illustrate 99mTc-labeled MB targeting and distribution in an experimental in vivo model of inflammation.\n\nFlow cytometry and confocal microscopy showed that binding of antibody-targeted MBs to the adhesion molecules ICAM-1, VCAM-1, or E-selectin, expressed on cytokine-stimulated ECs, was up to sixfold higher for human and 12-fold higher for mouse ECs, compared with that of non-targeted MBs. Under flow conditions, both VCAM-1- and E-selectin-targeted MBs adhered more firmly to stimulated human ECs than to untreated cells, while VCAM-1-targeted MBs adhered best to stimulated murine ECs. SPECT imaging showed an approximate doubling of signal intensity from the abdomen of rats with peritonitis, compared with healthy controls, after injection of anti-ICAM-1-MBs.\n\nThis novel multilayer contrast agent can specifically target adhesion molecules expressed as a result of inflammatory stimuli in vitro, and has potential for use in disease-specific multimodal diagnostics in vivo using antibodies against targets of interest.", "doi": "10.1007/s12195-018-00562-z", "pmid": "31719897", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC6816780"}, {"db": "pii", "key": "562"}], "notes": [], "created": "2026-08-20T06:40:35.583Z", "modified": "2026-08-20T06:40:35.706Z"}]}