{"entity": "researcher", "timestamp": "2026-09-30T05:02:16.299Z", "family": "Nyman", "given": "Tuula A", "initials": "TA", "orcid": "0000-0001-8787-5886", "affiliations": ["Department of Immunology, Institute of Clinical Medicine, University of Oslo and Rikshospitalet Oslo, Oslo, Norway."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/844e902977a54f748440df5e94751610.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/844e902977a54f748440df5e94751610"}}, "publications": [{"entity": "publication", "iuid": "b614e0ff32ac49b19327bb893ac7a18d", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/b614e0ff32ac49b19327bb893ac7a18d.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/b614e0ff32ac49b19327bb893ac7a18d"}}, "title": "Splicing of platelet resident pre-mRNAs upon activation by physiological stimuli results in functionally relevant proteome modifications.", "authors": [{"family": "Nassa", "given": "Giovanni", "initials": "G", "orcid": "0000-0001-7453-1240", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f25fa9bfad714097bf08ab09087017b0.json"}}, {"family": "Giurato", "given": "Giorgio", "initials": "G"}, {"family": "Cimmino", "given": "Giovanni", "initials": "G"}, {"family": "Rizzo", "given": "Francesca", "initials": "F"}, {"family": "Ravo", "given": "Maria", "initials": "M"}, {"family": "Salvati", "given": "Annamaria", "initials": "A"}, {"family": "Nyman", "given": "Tuula A", "initials": "TA", "orcid": "0000-0001-8787-5886", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/844e902977a54f748440df5e94751610.json"}}, {"family": "Zhu", "given": "Yafeng", "initials": "Y"}, {"family": "Vesterlund", "given": "Mattias", "initials": "M", "orcid": "0000-0001-9471-6592", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e443cdb01cc44a7fa786941b5c0b62d6.json"}}, {"family": "Lehti\u00f6", "given": "Janne", "initials": "J", "orcid": "0000-0002-8100-9562", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/561efcf32e2648c8a10fee692fc4e908.json"}}, {"family": "Golino", "given": "Paolo", "initials": "P"}, {"family": "Weisz", "given": "Alessandro", "initials": "A"}, {"family": "Tarallo", "given": "Roberta", "initials": "R", "orcid": "0000-0001-9668-3632", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a01e1b3dcc494ea785de9537e06e6152.json"}}], "type": "journal article", "published": "2018-01-11", "journal": {"title": "Sci Rep", "issn": "2045-2322", "volume": "8", "issue": "1", "pages": "498", "issn-l": "2045-2322"}, "abstract": "Platelet activation triggers thrombus formation in physiological and pathological conditions, such as acute coronary syndromes. Current therapies still fail to prevent thrombotic events in numerous patients, indicating that the mechanisms modulating platelet response during activation need to be clarified. The evidence that platelets are capable of de novo protein synthesis in response to stimuli raised the issue of how megakaryocyte-derived mRNAs are regulated in these anucleate cell fragments. Proteogenomics was applied here to investigate this phenomeon in platelets activated in vitro with Collagen or Thrombin Receptor Activating Peptide. Combining proteomics and transcriptomics allowed in depth platelet proteome characterization, revealing a significant effect of either stimulus on proteome composition. In silico analysis revealed the presence of resident immature RNAs in resting platelets, characterized by retained introns, while unbiased proteogenomics correlated intron removal by RNA splicing with changes on proteome composition upon activation. This allowed identification of a set of transcripts undergoing maturation by intron removal during activation and resulting in accumulation of the corresponding peptides at exon-exon junctions. These results indicate that RNA splicing events occur in platelets during activation and that maturation of specific pre-mRNAs is part of the activation cascade, contributing to a dynamic fine-tuning of the transcriptome.", "doi": "10.1038/s41598-017-18985-5", "pmid": "29323256", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC5765118"}, {"db": "pii", "key": "10.1038/s41598-017-18985-5"}], "notes": [], "created": "2018-12-05T12:24:51.064Z", "modified": "2026-09-23T07:47:28.038Z"}, {"entity": "publication", "iuid": "f7e39126580c4dda9c04c4b8123c720e", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/f7e39126580c4dda9c04c4b8123c720e.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/f7e39126580c4dda9c04c4b8123c720e"}}, "title": "Antiviral Properties of Chemical Inhibitors of Cellular Anti-Apoptotic Bcl-2 Proteins.", "authors": [{"family": "Bulanova", "given": "Daria", "initials": "D"}, {"family": "Ianevski", "given": "Aleksandr", "initials": "A"}, {"family": "Bugai", "given": "Andrii", "initials": "A", "orcid": "0000-0003-1920-6044", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/838b8d2e2cec452c9db58c6f521c1563.json"}}, {"family": "Akimov", "given": "Yevhen", "initials": "Y"}, {"family": "Kuivanen", "given": "Suvi", "initials": "S"}, {"family": "Paavilainen", "given": "Henrik", "initials": "H"}, {"family": "Kakkola", "given": "Laura", "initials": "L"}, {"family": "Nandania", "given": "Jatin", "initials": "J"}, {"family": "Turunen", "given": "Laura", "initials": "L"}, {"family": "Ohman", "given": "Tiina", "initials": "T"}, {"family": "Ala-Hongisto", "given": "Hanna", "initials": "H"}, {"family": "Pesonen", "given": "Hanna M", "initials": "HM"}, {"family": "Kuisma", "given": "Marika S", "initials": "MS"}, {"family": "Honkimaa", "given": "Anni", "initials": "A"}, {"family": "Walton", "given": "Emma L", "initials": "EL"}, {"family": "Oksenych", "given": "Valentyn", "initials": "V"}, {"family": "Lorey", "given": "Martina B", "initials": "MB", "orcid": "0000-0001-5605-6714", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/51272a61ecac4bb68294f171d17ecb1c.json"}}, {"family": "Guschin", "given": "Dmitry", "initials": "D"}, {"family": "Shim", "given": "Jungmin", "initials": "J"}, {"family": "Kim", "given": "Jinhee", "initials": "J"}, {"family": "Than", "given": "Thoa T", "initials": "TT"}, {"family": "Chang", "given": "So Young", "initials": "SY"}, {"family": "Hukkanen", "given": "Veijo", "initials": "V"}, {"family": "Kulesskiy", "given": "Evgeny", "initials": "E"}, {"family": "Marjomaki", "given": "Varpu S", "initials": "VS", "orcid": "0000-0002-4592-5926", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d311777ba9e24ca086664cf8311f7823.json"}}, {"family": "Julkunen", "given": "Ilkka", "initials": "I"}, {"family": "Nyman", "given": "Tuula A", "initials": "TA", "orcid": "0000-0001-8787-5886", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/844e902977a54f748440df5e94751610.json"}}, {"family": "Matikainen", "given": "Sampsa", "initials": "S"}, {"family": "Saarela", "given": "Jani S", "initials": "JS"}, {"family": "Sane", "given": "Famara", "initials": "F"}, {"family": "Hober", "given": "Didier", "initials": "D"}, {"family": "Gabriel", "given": "G\u00fclsah", "initials": "G"}, {"family": "De Brabander", "given": "Jef K", "initials": "JK"}, {"family": "Martikainen", "given": "Miika", "initials": "M"}, {"family": "Windisch", "given": "Marc P", "initials": "MP"}, {"family": "Min", "given": "Ji-Young", "initials": "JY"}, {"family": "Bruzzone", "given": "Roberto", "initials": "R"}, {"family": "Aittokallio", "given": "Tero", "initials": "T", "orcid": "0000-0002-0886-9769", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/433e429e6a5b49818cf54f5fc102fff7.json"}}, {"family": "V\u00e4h\u00e4-Koskela", "given": "Markus", "initials": "M"}, {"family": "Vapalahti", "given": "Olli", "initials": "O"}, {"family": "Pulk", "given": "Arto", "initials": "A"}, {"family": "Velagapudi", "given": "Vidya", "initials": "V"}, {"family": "Kainov", "given": "Denis E", "initials": "DE", "orcid": "0000-0001-7716-6955", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f1a2a96fa7cc4876bcdd781fef13e93e.json"}}], "type": "journal article", "published": "2017-09-25", "journal": {"title": "Viruses", "issn": "1999-4915", "volume": "9", "issue": "10", "issn-l": "1999-4915"}, "abstract": "Viral diseases remain serious threats to public health because of the shortage of effective means of control. To combat the surge of viral diseases, new treatments are urgently needed. Here we show that small-molecules, which inhibit cellular anti-apoptotic Bcl-2 proteins (Bcl-2i), induced the premature death of cells infected with different RNA or DNA viruses, whereas, at the same concentrations, no toxicity was observed in mock-infected cells. Moreover, these compounds limited viral replication and spread. Surprisingly, Bcl-2i also induced the premature apoptosis of cells transfected with viral RNA or plasmid DNA but not of mock-transfected cells. These results suggest that Bcl-2i sensitizes cells containing foreign RNA or DNA to apoptosis. A comparison of the toxicity, antiviral activity, and side effects of six Bcl-2i allowed us to select A-1155463 as an antiviral lead candidate. Thus, our results pave the way for the further development of Bcl-2i for the prevention and treatment of viral diseases.", "doi": "10.3390/v9100271", "pmid": "28946654", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC5691623"}, {"db": "pii", "key": "v9100271"}], "notes": [], "created": "2018-12-05T12:54:07.585Z", "modified": "2026-09-23T08:42:52.101Z"}]}