{"entity": "researcher", "timestamp": "2026-09-30T00:47:44.066Z", "family": "Aerts", "given": "Jordan T", "initials": "JT", "orcid": "0000-0001-9554-1966", "affiliations": ["Department of Pharmaceutical Biosciences, Uppsala University, Uppsala, Sweden."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/7f88b245d1784940abcd69f283bebcf4.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/7f88b245d1784940abcd69f283bebcf4"}}, "publications": [{"entity": "publication", "iuid": "3c2678988bb64aa98934a5df655a83d0", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/3c2678988bb64aa98934a5df655a83d0.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/3c2678988bb64aa98934a5df655a83d0"}}, "title": "Rapid Metabolic Profiling of 1 \u03bcL Crude Cerebrospinal Fluid by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging Can Differentiate De Novo Parkinson's Disease.", "authors": [{"family": "Vallianatou", "given": "Theodosia", "initials": "T"}, {"family": "Nilsson", "given": "Anna", "initials": "A"}, {"family": "Bj\u00e4rterot", "given": "Patrik", "initials": "P"}, {"family": "Shariatgorji", "given": "Reza", "initials": "R"}, {"family": "Slijkhuis", "given": "Nuria", "initials": "N"}, {"family": "Aerts", "given": "Jordan T", "initials": "JT", "orcid": "0000-0001-9554-1966", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/7f88b245d1784940abcd69f283bebcf4.json"}}, {"family": "Jansson", "given": "Erik T", "initials": "ET", "orcid": "0000-0002-0675-3412", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5982be2a4d6845339ff1964bf2dcb77c.json"}}, {"family": "Svenningsson", "given": "Per", "initials": "P"}, {"family": "Andr\u00e9n", "given": "Per E", "initials": "PE", "orcid": "0000-0002-4062-7743", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f1dd27e8b238475383983480e10099f6.json"}}], "type": "journal article", "published": "2023-12-19", "journal": {"title": "Anal. Chem.", "issn": "1520-6882", "volume": "95", "issue": "50", "pages": "18352-18360", "issn-l": "0003-2700"}, "abstract": "Parkinson's disease (PD) is a highly prevalent neurodegenerative disorder affecting the motor system. However, the correct diagnosis of PD and atypical parkinsonism may be difficult with high clinical uncertainty. There is an urgent need to identify reliable biomarkers using high-throughput, molecular-specific methods to improve current diagnostics. Here, we present a matrix-assisted laser desorption/ionization mass spectrometry imaging method that requires minimal sample preparation and only 1 \u03bcL of crude cerebrospinal fluid (CSF). The method enables analysis of hundreds of samples in a single experiment while simultaneously detecting numerous metabolites with subppm mass accuracy. To test the method, we analyzed CSF samples from 12 de novo PD patients (that is, newly diagnosed and previously untreated) and 12 age-matched controls. Within the identified molecules, we found neurotransmitters and their metabolites such as \u03b3-aminobutyric acid, 3-methoxytyramine, homovanillic acid, serotonin, histamine, amino acids, and metabolic intermediates. Limits of detection were estimated for multiple neurotransmitters with high linearity (R2 > 0.99) and sensitivity (as low as 16 pg/\u03bcL). Application of multivariate classification led to a highly significant (P < 0.001) model of PD prediction with a 100% classification rate, which was further thoroughly validated with a permutation test and univariate analysis. Molecules related to the neuromelanin pathway were found to be significantly increased in the PD group, indicated by their elevated relative intensities compared to the control group. Our method enables rapid detection of PD-related biomarkers in low sample volumes and could serve as a valuable tool in the development of robust PD diagnostics.", "doi": "10.1021/acs.analchem.3c02900", "pmid": "38059473", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC10733901"}], "notes": [], "created": "2026-09-23T11:24:02.270Z", "modified": "2026-09-23T11:24:02.308Z"}, {"entity": "publication", "iuid": "6928bfa004e844f4bb6f765ec380060f", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/6928bfa004e844f4bb6f765ec380060f.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/6928bfa004e844f4bb6f765ec380060f"}}, "title": "Enantioselective CE-MS analysis of ketamine metabolites in urine.", "authors": [{"family": "Sandbaumh\u00fcter", "given": "Friederike A", "initials": "FA", "orcid": "0000-0003-3391-3247", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9683ab81763444d29ce39f3fed737cf7.json"}}, {"family": "Aerts", "given": "Jordan T", "initials": "JT", "orcid": "0000-0001-9554-1966", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/7f88b245d1784940abcd69f283bebcf4.json"}}, {"family": "Theurillat", "given": "Regula", "initials": "R"}, {"family": "Andr\u00e9n", "given": "Per E", "initials": "PE", "orcid": "0000-0002-4062-7743", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f1dd27e8b238475383983480e10099f6.json"}}, {"family": "Thormann", "given": "Wolfgang", "initials": "W", "orcid": "0000-0002-9762-1609", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/364b9ad4e3fb4e02bc083b261fab466f.json"}}, {"family": "Jansson", "given": "Erik T", "initials": "ET", "orcid": "0000-0002-0675-3412", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5982be2a4d6845339ff1964bf2dcb77c.json"}}], "type": "journal article", "published": "2023-01-00", "journal": {"title": "Electrophoresis", "issn": "1522-2683", "volume": "44", "issue": "1-2", "pages": "125-134", "issn-l": "0173-0835"}, "abstract": "The chiral drug ketamine has long-lasting antidepressant effects with a fast onset and is also suitable to treat patients with therapy-resistant depression. The metabolite hydroxynorketamine (HNK) plays an important role in the antidepressant mechanism of action. Hydroxylation at the cyclohexanone ring occurs at positions 4, 5, and 6 and produces a total of 12 stereoisomers. Among those, the four 6HNK stereoisomers have the strongest antidepressant effects. Capillary electrophoresis with highly sulfated \u03b3-cyclodextrin (CD) as a chiral selector in combination with mass spectrometry (MS) was used to develop a method for the enantioselective analysis of HNK stereoisomers with a special focus on the 6HNK stereoisomers. The partial filling approach was applied in order to avoid contamination of the MS with the chiral selector. Concentration of the chiral selector and the length of the separation zone were optimized. With 5% highly sulfated \u03b3-CD in 20 mM ammonium formate with 10% formic acid and a 75% filling the four 6HNK stereoisomers could be separated with a resolution between 0.79 and 3.17. The method was applied to analyze fractionated equine urine collected after a ketamine infusion and to screen the fractions as well as unfractionated urine for the parent drug ketamine and other metabolites, including norketamine and dehydronorketamine.", "doi": "10.1002/elps.202200175", "pmid": "36398998", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC10108174"}], "notes": [], "created": "2026-09-23T11:03:49.692Z", "modified": "2026-09-23T11:03:49.884Z"}]}