{"entity": "researcher", "timestamp": "2026-11-12T14:02:15.845Z", "family": "Garc\u00eda-Sastre", "given": "Adolfo", "initials": "A", "orcid": "0000-0002-6551-1827", "affiliations": ["Quantitative Biosciences Institute (QBI) Coronavirus Research Group (QCRG), San Francisco, CA, 94158, USA. adolfo.garcia-sastre@mssm.edu.", "Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, 10029, USA. adolfo.garcia-sastre@mssm.edu.", "Global Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York, NY, 10029, USA. adolfo.garcia-sastre@mssm.edu.", "Department of Medicine, Division of Infectious Diseases, Icahn School of Medicine at Mount Sinai, New York, NY, 10029, USA. adolfo.garcia-sastre@mssm.edu.", "Department of Pathology, Molecular and Cell-Based Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, 10029, USA. adolfo.garcia-sastre@mssm.edu.", "The Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY, 10029, USA. adolfo.garcia-sastre@mssm.edu."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/7d9ee4b066f24fe7b2e488c00fac4938.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/7d9ee4b066f24fe7b2e488c00fac4938"}}, "publications": [{"entity": "publication", "iuid": "df9e37fcc38244128dacc5a41ba38be1", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/df9e37fcc38244128dacc5a41ba38be1.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/df9e37fcc38244128dacc5a41ba38be1"}}, "title": "Proteomic and genetic analyses of influenza A viruses identify pan-viral host targets.", "authors": [{"family": "Haas", "given": "Kelsey M", "initials": "KM", "orcid": "0000-0002-1463-6726", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/2b50e67ced164f2d8e825f58406b3c66.json"}}, {"family": "McGregor", "given": "Michael J", "initials": "MJ"}, {"family": "Bouhaddou", "given": "Mehdi", "initials": "M"}, {"family": "Polacco", "given": "Benjamin J", "initials": "BJ", "orcid": "0000-0003-1570-9234", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9b41eced550240729eae78f2d9864552.json"}}, {"family": "Kim", "given": "Eun-Young", "initials": "EY", "orcid": "0000-0002-7436-7396", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f919c62cc14542e39ee29138bbba1421.json"}}, {"family": "Nguyen", "given": "Thong T", "initials": "TT"}, {"family": "Newton", "given": "Billy W", "initials": "BW"}, {"family": "Urbanowski", "given": "Matthew", "initials": "M"}, {"family": "Kim", "given": "Heejin", "initials": "H"}, {"family": "Williams", "given": "Michael A P", "initials": "MAP"}, {"family": "Rezelj", "given": "Veronica V", "initials": "VV"}, {"family": "Hardy", "given": "Alexandra", "initials": "A"}, {"family": "Fossati", "given": "Andrea", "initials": "A"}, {"family": "Stevenson", "given": "Erica J", "initials": "EJ"}, {"family": "Sukerman", "given": "Ellie", "initials": "E"}, {"family": "Kim", "given": "Tiffany", "initials": "T", "orcid": "0000-0001-8386-7686", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6b5f94b9170241e985ef81d6fbeaf601.json"}}, {"family": "Penugonda", "given": "Sudhir", "initials": "S"}, {"family": "Moreno", "given": "Elena", "initials": "E", "orcid": "0000-0002-2301-4558", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/96ac76807a374faabd225a4130e77200.json"}}, {"family": "Braberg", "given": "Hannes", "initials": "H", "orcid": "0000-0002-7070-2257", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c171b1abb0994aecbb58f3b6fc69986f.json"}}, {"family": "Zhou", "given": "Yuan", "initials": "Y"}, {"family": "Metreveli", "given": "Giorgi", "initials": "G"}, {"family": "Harjai", "given": "Bhavya", "initials": "B"}, {"family": "Tummino", "given": "Tia A", "initials": "TA", "orcid": "0000-0001-9155-820X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/37c7a6f8c3dc4306bc33ae11425e28db.json"}}, {"family": "Melnyk", "given": "James E", "initials": "JE", "orcid": "0000-0003-1915-8089", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9fe7e99aadf040068b03a5bc37a7c905.json"}}, {"family": "Soucheray", "given": "Margaret", "initials": "M", "orcid": "0000-0002-0370-6653", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4551bed353dc4d21af7e04d7c19d1a05.json"}}, {"family": "Batra", "given": "Jyoti", "initials": "J"}, {"family": "Pache", "given": "Lars", "initials": "L", "orcid": "0000-0002-4193-1042", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/02388f43a7dd4eaa81542009390b2628.json"}}, {"family": "Martin-Sancho", "given": "Laura", "initials": "L"}, {"family": "Carlson-Stevermer", "given": "Jared", "initials": "J"}, {"family": "Jureka", "given": "Alexander S", "initials": "AS"}, {"family": "Basler", "given": "Christopher F", "initials": "CF", "orcid": "0000-0003-4195-425X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/990e8bbaa2e94a1796eb15a4fd427480.json"}}, {"family": "Shokat", "given": "Kevan M", "initials": "KM", "orcid": "0000-0001-8590-7741", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e37dd23f9a71424d8f2c804f27fc86e7.json"}}, {"family": "Shoichet", "given": "Brian K", "initials": "BK", "orcid": "0000-0002-6098-7367", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/dc2c3cf99cb740adaddba8d5227e884b.json"}}, {"family": "Shriver", "given": "Leah P", "initials": "LP"}, {"family": "Johnson", "given": "Jeffrey R", "initials": "JR"}, {"family": "Shaw", "given": "Megan L", "initials": "ML", "orcid": "0000-0002-1267-0891", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/39db515aba5e4e869633bf625deb923f.json"}}, {"family": "Chanda", "given": "Sumit K", "initials": "SK", "orcid": "0000-0001-9399-7927", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b2dca57069e941ad81d04edcef1aeb90.json"}}, {"family": "Roden", "given": "Dan M", "initials": "DM"}, {"family": "Carter", "given": "Tonia C", "initials": "TC", "orcid": "0000-0002-2107-7838", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/80373483bfcf4854971ed3e439432a67.json"}}, {"family": "Kottyan", "given": "Leah C", "initials": "LC", "orcid": "0000-0003-3979-2220", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/77c564dfd41e45cd9f769e216abed37b.json"}}, {"family": "Chisholm", "given": "Rex L", "initials": "RL"}, {"family": "Pacheco", "given": "Jennifer A", "initials": "JA", "orcid": "0000-0001-8021-5818", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/188786633dac4b139f77ea72d4ad5bc3.json"}}, {"family": "Smith", "given": "Maureen E", "initials": "ME", "orcid": "0000-0001-9480-1092", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4fca60b39c1e484bb472957b7263df54.json"}}, {"family": "Schrodi", "given": "Steven J", "initials": "SJ", "orcid": "0000-0003-2304-8528", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/effefd678848415f8f170ff06f8a7ef7.json"}}, {"family": "Albrecht", "given": "Randy A", "initials": "RA", "orcid": "0000-0003-4008-503X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ba8a641dc3d843f19ceb34d75f74af5d.json"}}, {"family": "Vignuzzi", "given": "Marco", "initials": "M"}, {"family": "Zuliani-Alvarez", "given": "Lorena", "initials": "L"}, {"family": "Swaney", "given": "Danielle L", "initials": "DL", "orcid": "0000-0001-6119-6084", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/56317207941d4aaf81ab4d55de5fcba4.json"}}, {"family": "Eckhardt", "given": "Manon", "initials": "M", "orcid": "0000-0001-8143-6129", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1e3597e5c67842f2930a6e08173590e9.json"}}, {"family": "Wolinsky", "given": "Steven M", "initials": "SM"}, {"family": "White", "given": "Kris M", "initials": "KM", "orcid": "0000-0003-0889-0506", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/7b428159f7954cc1addd247d332ec355.json"}}, {"family": "Hultquist", "given": "Judd F", "initials": "JF", "orcid": "0000-0001-6424-4280", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/984045e2941a49bb93ffbbcd639264da.json"}}, {"family": "Kaake", "given": "Robyn M", "initials": "RM"}, {"family": "Garc\u00eda-Sastre", "given": "Adolfo", "initials": "A", "orcid": "0000-0002-6551-1827", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/7d9ee4b066f24fe7b2e488c00fac4938.json"}}, {"family": "Krogan", "given": "Nevan J", "initials": "NJ", "orcid": "0000-0003-4902-337X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/bf20e1447b9643f599f94d983b2bd9dd.json"}}], "type": "journal article", "published": "2023-09-27", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "14", "issue": "1", "pages": "6030", "issn-l": "2041-1723"}, "abstract": "Influenza A Virus (IAV) is a recurring respiratory virus with limited availability of antiviral therapies. Understanding host proteins essential for IAV infection can identify targets for alternative host-directed therapies (HDTs). Using affinity purification-mass spectrometry and global phosphoproteomic and protein abundance analyses using three IAV strains (pH1N1, H3N2, H5N1) in three human cell types (A549, NHBE, THP-1), we map 332 IAV-human protein-protein interactions and identify 13 IAV-modulated kinases. Whole exome sequencing of patients who experienced severe influenza reveals several genes, including scaffold protein AHNAK, with predicted loss-of-function variants that are also identified in our proteomic analyses. Of our identified host factors, 54 significantly alter IAV infection upon siRNA knockdown, and two factors, AHNAK and coatomer subunit COPB1, are also essential for productive infection by SARS-CoV-2. Finally, 16 compounds targeting our identified host factors suppress IAV replication, with two targeting CDK2 and FLT3 showing pan-antiviral activity across influenza and coronavirus families. This study provides a comprehensive network model of IAV infection in human cells, identifying functional host targets for pan-viral HDT.", "doi": "10.1038/s41467-023-41442-z", "pmid": "37758692", "labels": {"Andrea Fossati": null, "DDLS Fellow": null}, "xrefs": [{"db": "pmc", "key": "PMC10533562"}, {"db": "pii", "key": "10.1038/s41467-023-41442-z"}], "notes": [], "created": "2026-11-06T08:03:37.050Z", "modified": "2026-11-06T08:03:38.500Z"}, {"entity": "publication", "iuid": "207c79bd8fd6481fa1d662ee7c308914", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/207c79bd8fd6481fa1d662ee7c308914.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/207c79bd8fd6481fa1d662ee7c308914"}}, "title": "Evolution of enhanced innate immune evasion by SARS-CoV-2.", "authors": [{"family": "Thorne", "given": "Lucy G", "initials": "LG", "orcid": "0000-0001-7358-6047", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/377d4c11a8174452908217e8ac3cb0ff.json"}}, {"family": "Bouhaddou", "given": "Mehdi", "initials": "M"}, {"family": "Reuschl", "given": "Ann-Kathrin", "initials": "AK"}, {"family": "Zuliani-Alvarez", "given": "Lorena", "initials": "L"}, {"family": "Polacco", "given": "Ben", "initials": "B", "orcid": "0000-0003-1570-9234", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9b41eced550240729eae78f2d9864552.json"}}, {"family": "Pelin", "given": "Adrian", "initials": "A"}, {"family": "Batra", "given": "Jyoti", "initials": "J"}, {"family": "Whelan", "given": "Matthew V X", "initials": "MVX"}, {"family": "Hosmillo", "given": "Myra", "initials": "M"}, {"family": "Fossati", "given": "Andrea", "initials": "A", "orcid": "0000-0001-5170-4903", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/658637b885384441943698722fad5944.json"}}, {"family": "Ragazzini", "given": "Roberta", "initials": "R", "orcid": "0000-0003-2186-293X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/977193338736455da68732317dc4220e.json"}}, {"family": "Jungreis", "given": "Irwin", "initials": "I", "orcid": "0000-0002-3197-5367", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/56f0daf23c054c59a0f58969dd5ac6db.json"}}, {"family": "Ummadi", "given": "Manisha", "initials": "M"}, {"family": "Rojc", "given": "Ajda", "initials": "A"}, {"family": "Turner", "given": "Jane", "initials": "J"}, {"family": "Bischof", "given": "Marie L", "initials": "ML"}, {"family": "Obernier", "given": "Kirsten", "initials": "K", "orcid": "0000-0002-4025-1299", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d2e68afc66484175b3d38a74979f8b4e.json"}}, {"family": "Braberg", "given": "Hannes", "initials": "H", "orcid": "0000-0002-7070-2257", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c171b1abb0994aecbb58f3b6fc69986f.json"}}, {"family": "Soucheray", "given": "Margaret", "initials": "M"}, {"family": "Richards", "given": "Alicia", "initials": "A"}, {"family": "Chen", "given": "Kuei-Ho", "initials": "KH", "orcid": "0000-0001-6541-0578", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/82abbba3d1394b6e983eb8395fdd9131.json"}}, {"family": "Harjai", "given": "Bhavya", "initials": "B"}, {"family": "Memon", "given": "Danish", "initials": "D", "orcid": "0000-0002-1365-0710", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0f9e1ae20abe4f7f94af1f6856d026bc.json"}}, {"family": "Hiatt", "given": "Joseph", "initials": "J"}, {"family": "Rosales", "given": "Romel", "initials": "R"}, {"family": "McGovern", "given": "Briana L", "initials": "BL", "orcid": "0000-0002-0492-9904", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c0c7155e8fca40789c7f3f483343562e.json"}}, {"family": "Jahun", "given": "Aminu", "initials": "A", "orcid": "0000-0002-4585-1701", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/92473a7f130640deae14cbab26e3f95a.json"}}, {"family": "Fabius", "given": "Jacqueline M", "initials": "JM"}, {"family": "White", "given": "Kris", "initials": "K", "orcid": "0000-0003-0889-0506", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/7b428159f7954cc1addd247d332ec355.json"}}, {"family": "Goodfellow", "given": "Ian G", "initials": "IG", "orcid": "0000-0002-9483-510X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/aa80c97bf6ee4b0f9359047f7e5db404.json"}}, {"family": "Takeuchi", "given": "Yasu", "initials": "Y"}, {"family": "Bonfanti", "given": "Paola", "initials": "P", "orcid": "0000-0001-9655-3766", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/48165c07f21245a4a21569c786a30085.json"}}, {"family": "Shokat", "given": "Kevan", "initials": "K", "orcid": "0000-0001-8590-7741", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e37dd23f9a71424d8f2c804f27fc86e7.json"}}, {"family": "Jura", "given": "Natalia", "initials": "N", "orcid": "0000-0001-5129-641X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d6751d2c23804f56a1d117bc2734e997.json"}}, {"family": "Verba", "given": "Klim", "initials": "K", "orcid": "0000-0002-2238-8590", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/fc38826304dc47ce9aee7ab94ce37561.json"}}, {"family": "Noursadeghi", "given": "Mahdad", "initials": "M", "orcid": "0000-0002-4774-0853", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/83695a663ced442aa01ad9b6f877ce8f.json"}}, {"family": "Beltrao", "given": "Pedro", "initials": "P"}, {"family": "Kellis", "given": "Manolis", "initials": "M", "orcid": "0000-0001-7113-9630", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9989fea905234f91aa4412731b93ab09.json"}}, {"family": "Swaney", "given": "Danielle L", "initials": "DL", "orcid": "0000-0001-6119-6084", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/56317207941d4aaf81ab4d55de5fcba4.json"}}, {"family": "Garc\u00eda-Sastre", "given": "Adolfo", "initials": "A", "orcid": "0000-0002-6551-1827", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/7d9ee4b066f24fe7b2e488c00fac4938.json"}}, {"family": "Jolly", "given": "Clare", "initials": "C", "orcid": "0000-0002-4603-2281", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/fbc228b022604eb087bc0a2d2d7b21a3.json"}}, {"family": "Towers", "given": "Greg J", "initials": "GJ", "orcid": "0000-0002-7707-0264", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e979e1fe50944178a4ea51b9d146dc95.json"}}, {"family": "Krogan", "given": "Nevan J", "initials": "NJ", "orcid": "0000-0003-4902-337X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/bf20e1447b9643f599f94d983b2bd9dd.json"}}], "type": "journal article", "published": "2022-02-00", "journal": {"title": "Nature", "issn": "1476-4687", "volume": "602", "issue": "7897", "pages": "487-495", "issn-l": "0028-0836"}, "abstract": "The emergence of SARS-CoV-2 variants of concern suggests viral adaptation to enhance human-to-human transmission1,2. Although much effort has focused on the characterization of changes in the spike protein in variants of concern, mutations outside of spike are likely to contribute to adaptation. Here, using unbiased abundance proteomics, phosphoproteomics, RNA sequencing and viral replication assays, we show that isolates of the Alpha (B.1.1.7) variant3 suppress innate immune responses in airway epithelial cells more effectively than first-wave isolates. We found that the Alpha variant has markedly increased subgenomic RNA and protein levels of the nucleocapsid protein (N), Orf9b and Orf6-all known innate immune antagonists. Expression of Orf9b alone suppressed the innate immune response through interaction with TOM70, a mitochondrial protein that is required for activation of the RNA-sensing adaptor MAVS. Moreover, the activity of Orf9b and its association with TOM70 was regulated by phosphorylation. We propose that more effective innate immune suppression, through enhanced expression of specific viral antagonist proteins, increases the likelihood of successful transmission of the Alpha variant, and may increase in vivo replication and duration of infection4. The importance of mutations outside the spike coding region in the adaptation of SARS-CoV-2 to humans is underscored by the observation that similar mutations exist in the N and Orf9b regulatory regions of the Delta and Omicron variants.", "doi": "10.1038/s41586-021-04352-y", "pmid": "34942634", "labels": {"Andrea Fossati": null, "DDLS Fellow": null}, "xrefs": [{"db": "mid", "key": "NIHMS1780228"}, {"db": "pmc", "key": "PMC8850198"}, {"db": "pii", "key": "10.1038/s41586-021-04352-y"}], "notes": [], "created": "2026-11-06T08:03:44.355Z", "modified": "2026-11-06T08:03:45.294Z"}]}