{"entity": "researcher", "timestamp": "2026-08-26T22:46:48.482Z", "family": "Lawson", "given": "Michael J", "initials": "MJ", "orcid": "0000-0002-2868-733X", "affiliations": ["Department of Cell and Molecular Biology, SciLifeLab, Uppsala University, Uppsala, Sweden. lawsonjmichael@gmail.com.", "Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA. lawsonjmichael@gmail.com."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/7b13ef7ad1d14886809bfb3aafefb8d2.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/7b13ef7ad1d14886809bfb3aafefb8d2"}}, "publications": [{"entity": "publication", "iuid": "b261a8dff79843e4b50b37a829339303", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/b261a8dff79843e4b50b37a829339303.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/b261a8dff79843e4b50b37a829339303"}}, "title": "Imaging-based screens of pool-synthesized cell libraries.", "authors": [{"family": "Lawson", "given": "Michael", "initials": "M", "orcid": "0000-0002-2868-733X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/7b13ef7ad1d14886809bfb3aafefb8d2.json"}}, {"family": "Elf", "given": "Johan", "initials": "J", "orcid": "0000-0001-5522-1810", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/7001138c10bf4a6d84075057ba30c8b9.json"}}], "type": "journal article", "published": "2021-04-00", "journal": {"title": "Nat. Methods", "issn": "1548-7105", "volume": "18", "issue": "4", "pages": "358-365", "issn-l": "1548-7091"}, "abstract": "Mapping a genetic perturbation to a change in phenotype is at the core of biological research. Advances in microscopy have transformed these studies, but they have largely been confined to examining a few strains or cell lines at a time. In parallel, there has been a revolution in creating synthetic libraries of genetically altered cells with relative ease. Here we describe methods that combine these powerful tools to perform live-cell imaging of pool-generated strain libraries for improved biological discovery.", "doi": "10.1038/s41592-020-01053-8", "pmid": "33589838", "labels": [], "xrefs": [{"db": "pii", "key": "10.1038/s41592-020-01053-8"}], "notes": [], "created": "2026-08-20T09:02:29.211Z", "modified": "2026-08-20T09:02:29.295Z"}, {"entity": "publication", "iuid": "e1c9386724ed4962868530b6ff82faec", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/e1c9386724ed4962868530b6ff82faec.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/e1c9386724ed4962868530b6ff82faec"}}, "title": "Time-resolved imaging-based CRISPRi screening.", "authors": [{"family": "Camsund", "given": "Daniel", "initials": "D", "orcid": "0000-0001-7471-7539", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ec47a9765d9b444ab5b73a9813a96130.json"}}, {"family": "Lawson", "given": "Michael J", "initials": "MJ", "orcid": "0000-0002-2868-733X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/7b13ef7ad1d14886809bfb3aafefb8d2.json"}}, {"family": "Larsson", "given": "Jimmy", "initials": "J"}, {"family": "Jones", "given": "Daniel", "initials": "D"}, {"family": "Zikrin", "given": "Spartak", "initials": "S"}, {"family": "Fange", "given": "David", "initials": "D"}, {"family": "Elf", "given": "Johan", "initials": "J", "orcid": "0000-0001-5522-1810", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/7001138c10bf4a6d84075057ba30c8b9.json"}}], "type": "journal article", "published": "2020-01-00", "journal": {"title": "Nat. Methods", "issn": "1548-7105", "volume": "17", "issue": "1", "pages": "86-92", "issn-l": "1548-7091"}, "abstract": "Our ability to connect genotypic variation to biologically important phenotypes has been seriously limited by the gap between live-cell microscopy and library-scale genomic engineering. Here, we show how in situ genotyping of a library of strains after time-lapse imaging in a microfluidic device overcomes this problem. We determine how 235 different CRISPR interference knockdowns impact the coordination of the replication and division cycles of Escherichia coli by monitoring the location of replication forks throughout on average >500 cell cycles per knockdown. Subsequent in situ genotyping allows us to map each phenotype distribution to a specific genetic perturbation to determine which genes are important for cell cycle control. The single-cell time-resolved assay allows us to determine the distribution of single-cell growth rates, cell division sizes and replication initiation volumes. The technology presented in this study enables genome-scale screens of most live-cell microscopy assays.", "doi": "10.1038/s41592-019-0629-y", "pmid": "31740817", "labels": [], "xrefs": [{"db": "pii", "key": "10.1038/s41592-019-0629-y"}], "notes": [], "created": "2026-08-20T09:02:17.496Z", "modified": "2026-08-20T09:02:17.647Z"}]}