{"entity": "researcher", "timestamp": "2026-08-28T00:11:50.442Z", "family": "Hansen", "given": "Henning Gram", "initials": "HG", "orcid": "0000-0002-2832-730X", "affiliations": ["The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kongens Lyngby, Denmark."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/79f654e67a19497d81c641a2a8d0aa34.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/79f654e67a19497d81c641a2a8d0aa34"}}, "publications": [{"entity": "publication", "iuid": "fcda2a00305b4517a8913ddbc95892db", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/fcda2a00305b4517a8913ddbc95892db.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/fcda2a00305b4517a8913ddbc95892db"}}, "title": "Chromophore pre-maturation for improved speed and sensitivity of split-GFP monitoring of protein secretion.", "authors": [{"family": "Lundqvist", "given": "Magnus", "initials": "M"}, {"family": "Thal\u00e9n", "given": "Niklas", "initials": "N"}, {"family": "Volk", "given": "Anna-Luisa", "initials": "AL"}, {"family": "Hansen", "given": "Henning Gram", "initials": "HG", "orcid": "0000-0002-2832-730X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/79f654e67a19497d81c641a2a8d0aa34.json"}}, {"family": "von Otter", "given": "Eric", "initials": "E"}, {"family": "Nygren", "given": "Per-\u00c5ke", "initials": "P\u00c5", "orcid": "0000-0003-4214-6991", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/db3f3b9b876c486e9c8acaa5efcfff18.json"}}, {"family": "Uhlen", "given": "Mathias", "initials": "M", "orcid": "0000-0002-4858-8056", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9ea446aa574042a295d5f69437402f76.json"}}, {"family": "Rockberg", "given": "Johan", "initials": "J"}], "type": "journal article", "published": "2019-01-22", "journal": {"title": "Sci Rep", "issn": "2045-2322", "volume": "9", "issue": "1", "pages": "310", "issn-l": "2045-2322"}, "abstract": "Complementation-dependent fluorescence is a powerful way to study co-localization or interactions between biomolecules. A split-GFP variant, involving the self-associating GFP 1-10 and GFP 11, has previously provided a convenient approach to measure recombinant protein titers in cell supernatants. A limitation of this approach is the slow chromophore formation after complementation. Here, we alleviate this lag in signal generation by allowing the GFP 1-10 chromophore to mature on a solid support containing GFP 11 before applying GFP 1-10 in analyses. The pre-maturated GFP 1-10 provided up to 150-fold faster signal generation compared to the non-maturated version. Moreover, pre-maturated GFP 1-10 significantly improved the ability of discriminating between Chinese hamster ovary (CHO) cell lines secreting GFP 11-tagged erythropoietin protein at varying rates. Its improved kinetics make the pre-maturated GFP 1-10 a suitable reporter molecule for cell biology research in general, especially for ranking individual cell lines based on secretion rates of recombinant proteins.", "doi": "10.1038/s41598-018-36559-x", "pmid": "30670736", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC6342966"}, {"db": "pii", "key": "10.1038/s41598-018-36559-x"}], "notes": [], "created": "2026-08-21T11:52:38.003Z", "modified": "2026-08-21T11:52:38.132Z"}]}