{"entity": "researcher", "timestamp": "2026-09-28T23:05:34.101Z", "family": "Krizhanovskii", "given": "Camilla", "initials": "C", "orcid": "0000-0003-2195-165X", "affiliations": ["a Science for Life Laboratory, Department of Medical Cell Biology , Uppsala University , Uppsala , Sweden.", "b S\u00f6dert\u00e4lje Hospital , Department of Internal Medicine , S\u00f6dert\u00e4lje , Sweden."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/728226ad3d0048eb8d63ee742cdb1b68.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/728226ad3d0048eb8d63ee742cdb1b68"}}, "publications": [{"entity": "publication", "iuid": "0472723878fe4092aa3b6adf12de4e06", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/0472723878fe4092aa3b6adf12de4e06.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/0472723878fe4092aa3b6adf12de4e06"}}, "title": "Addition of exogenous sodium palmitate increases the IAPP/insulin mRNA ratio via GPR40 in human EndoC-\u03b2H1 cells.", "authors": [{"family": "Krizhanovskii", "given": "Camilla", "initials": "C", "orcid": "0000-0003-2195-165X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/728226ad3d0048eb8d63ee742cdb1b68.json"}}, {"family": "Fred", "given": "Rikard G", "initials": "RG"}, {"family": "Oskarsson", "given": "Marie E", "initials": "ME"}, {"family": "Westermark", "given": "Gunilla T", "initials": "GT"}, {"family": "Welsh", "given": "Nils", "initials": "N"}], "type": "journal article", "published": "2017-08-00", "journal": {"title": "Ups. J. Med. Sci.", "issn": "2000-1967", "volume": "122", "issue": "3", "pages": "149-159", "issn-l": "0300-9734"}, "abstract": "Enhanced IAPP production may contribute to islet amyloid formation in type 2 diabetes. The objective of this study was to determine the effects of the saturated fatty acid palmitate on IAPP levels in human \u03b2-cells.\n\nEndoC-\u03b2H1 cells and human islets were cultured in the presence of sodium palmitate. Effects on IAPP/insulin mRNA expression and secretion were determined using real-time qPCR/ELISA. Pharmacological activators and/or inhibitors and RNAi were used to determine the underlying mechanisms.\n\nWe observed that EndoC-\u03b2H1 cells exposed to palmitate for 72 h displayed decreased expression of Pdx-1 and MafA and increased expression of thioredoxin-interacting protein (TXNIP), reduced insulin mRNA expression and glucose-induced insulin secretion, as well as increased IAPP mRNA expression and secretion. Further, these effects were independent of fatty acid oxidation, but abolished in response to GPR40 inhibition/downregulation. In human islets both a high glucose concentration and palmitate promoted increased IAPP mRNA levels, resulting in an augmented IAPP/insulin mRNA ratio. This was paralleled by elevated IAPP/insulin protein secretion and content ratios.\n\nAddition of exogenous palmitate to human \u03b2-cells increased the IAPP/insulin expression ratio, an effect contributed to by activation of GPR40. These findings may be pertinent to our understanding of the islet amyloid formation process.", "doi": "10.1080/03009734.2017.1368745", "pmid": "28980863", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC5649320"}], "notes": [], "created": "2018-12-05T12:08:29.603Z", "modified": "2026-09-23T12:04:01.572Z"}]}