{"entity": "researcher", "timestamp": "2026-08-20T20:40:05.885Z", "family": "Watzl", "given": "Carsten", "initials": "C", "orcid": "0000-0001-5195-0995", "affiliations": ["Department for Immunology, Leibniz Research Centre for Working Environment and Human Factors, TU Dortmund, Dortmund, Germany."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/705dc690627d4843a59d21e3f2b62ea6.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/705dc690627d4843a59d21e3f2b62ea6"}}, "publications": [{"entity": "publication", "iuid": "2e468fb6ee84403d9cddc6c5ef0a7713", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/2e468fb6ee84403d9cddc6c5ef0a7713.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/2e468fb6ee84403d9cddc6c5ef0a7713"}}, "title": "Modulation of lytic molecules restrain serial killing in \u03b3\u03b4 T lymphocytes.", "authors": [{"family": "Sandoz", "given": "Patrick A", "initials": "PA", "orcid": "0000-0002-8379-7267", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d309ec0cb74f41a49a18e226079e6787.json"}}, {"family": "Kuhnigk", "given": "Kyra", "initials": "K", "orcid": "0000-0002-2265-8204", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c9038f497daf47f4b001942f2d6a5788.json"}}, {"family": "Szabo", "given": "Edina K", "initials": "EK"}, {"family": "Thunberg", "given": "Sarah", "initials": "S", "orcid": "0000-0001-6912-6048", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4c1a27ca8cd2429098eeb6bbebaa73f5.json"}}, {"family": "Erikson", "given": "Elina", "initials": "E"}, {"family": "Sandstr\u00f6m", "given": "Niklas", "initials": "N", "orcid": "0000-0001-6443-878X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b65278c9896e466f92f62f3ba1e58136.json"}}, {"family": "Verron", "given": "Quentin", "initials": "Q", "orcid": "0000-0001-5800-4379", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/31de7b29ad984949bc1901417bc7ed25.json"}}, {"family": "Brech", "given": "Andreas", "initials": "A"}, {"family": "Watzl", "given": "Carsten", "initials": "C", "orcid": "0000-0001-5195-0995", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/705dc690627d4843a59d21e3f2b62ea6.json"}}, {"family": "Wagner", "given": "Arnika K", "initials": "AK"}, {"family": "Alici", "given": "Evren", "initials": "E"}, {"family": "Malmberg", "given": "Karl-Johan", "initials": "KJ", "orcid": "0000-0002-8718-9373", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e1b891c497ca402aaff79e8082f22553.json"}}, {"family": "Uhlin", "given": "Michael", "initials": "M", "orcid": "0000-0002-2177-6727", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/116cfe482bb941159ee2e7175f1cd095.json"}}, {"family": "\u00d6nfelt", "given": "Bj\u00f6rn", "initials": "B", "orcid": "0000-0001-5178-7593", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6b4b8fcbedb54e2ba38633687f417ae8.json"}}], "type": "journal article", "published": "2023-09-27", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "14", "issue": "1", "pages": "6035", "issn-l": "2041-1723"}, "abstract": "\u03b3\u03b4 T cells play a pivotal role in protection against various types of infections and tumours, from early childhood on and throughout life. They consist of several subsets characterised by adaptive and innate-like functions, with V\u03b39V\u03b42 being the largest subset in human peripheral blood. Although these cells show signs of cytotoxicity, their modus operandi remains poorly understood. Here we explore, using live single-cell imaging, the cytotoxic functions of \u03b3\u03b4 T cells upon interactions with tumour target cells with high temporal and spatial resolution. While \u03b3\u03b4 T cell killing is dominated by degranulation, the availability of lytic molecules appears tightly regulated in time and space. In particular, the limited co-occurrence of granzyme B and perforin restrains serial killing of tumour cells by \u03b3\u03b4 T cells. Thus, our data provide new insights into the cytotoxic arsenal and functions of \u03b3\u03b4 T cells, which may guide the development of more efficient \u03b3\u03b4 T cell based adoptive immunotherapies.", "doi": "10.1038/s41467-023-41634-7", "pmid": "37758698", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC10533871"}, {"db": "pii", "key": "10.1038/s41467-023-41634-7"}], "notes": [], "created": "2026-08-20T08:52:55.661Z", "modified": "2026-08-20T08:52:56.006Z"}, {"entity": "publication", "iuid": "9551a22f052a4e8eb02f43df52a2288c", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/9551a22f052a4e8eb02f43df52a2288c.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/9551a22f052a4e8eb02f43df52a2288c"}}, "title": "Modulation of lytic molecules restrain serial killing in \u03b3\u03b4 T lymphocytes", "authors": [{"family": "Onfelt", "given": "Bjorn", "initials": "B", "orcid": "0000-0001-5178-7593", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6b4b8fcbedb54e2ba38633687f417ae8.json"}}, {"family": "Sandoz", "given": "Patrick", "initials": "P"}, {"family": "Kuhnigk", "given": "Kyra", "initials": "K"}, {"family": "Szabo", "given": "Edina", "initials": "E"}, {"family": "Thunberg", "given": "Sarah", "initials": "S", "orcid": "0000-0001-6912-6048", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4c1a27ca8cd2429098eeb6bbebaa73f5.json"}}, {"family": "Erikson", "given": "Elina", "initials": "E"}, {"family": "Sandstr\u00f6m", "given": "Niklas", "initials": "N", "orcid": "0000-0001-6443-878X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b65278c9896e466f92f62f3ba1e58136.json"}}, {"family": "Verron", "given": "Quentin", "initials": "Q"}, {"family": "Brech", "given": "Andreas", "initials": "A"}, {"family": "Watzl", "given": "Carsten", "initials": "C", "orcid": "0000-0001-5195-0995", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/705dc690627d4843a59d21e3f2b62ea6.json"}}, {"family": "Wagner", "given": "Arnika", "initials": "A"}, {"family": "Alici", "given": "Evren", "initials": "E"}, {"family": "Malmberg", "given": "Karl Johan", "initials": "KJ", "orcid": "0000-0002-8718-9373", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e1b891c497ca402aaff79e8082f22553.json"}}, {"family": "Uhlin", "given": "Michael", "initials": "M", "orcid": "0000-0002-2177-6727", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/116cfe482bb941159ee2e7175f1cd095.json"}}], "type": "posted-content", "published": "2023-02-21", "journal": {"issn-l": null}, "abstract": null, "doi": "10.21203/rs.3.rs-2371463/v1", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T12:58:48.009Z", "modified": "2026-08-20T12:58:48.055Z"}, {"entity": "publication", "iuid": "e8ff69c347d040da88e3f25d7aafc45a", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/e8ff69c347d040da88e3f25d7aafc45a.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/e8ff69c347d040da88e3f25d7aafc45a"}}, "title": "NK cells switch from granzyme B to death receptor-mediated cytotoxicity during serial killing.", "authors": [{"family": "Prager", "given": "Isabel", "initials": "I"}, {"family": "Liesche", "given": "Clarissa", "initials": "C"}, {"family": "van Ooijen", "given": "Hanna", "initials": "H"}, {"family": "Urlaub", "given": "Doris", "initials": "D"}, {"family": "Verron", "given": "Quentin", "initials": "Q", "orcid": "0000-0001-5800-4379", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/31de7b29ad984949bc1901417bc7ed25.json"}}, {"family": "Sandstr\u00f6m", "given": "Niklas", "initials": "N"}, {"family": "Fasbender", "given": "Frank", "initials": "F"}, {"family": "Claus", "given": "Maren", "initials": "M", "orcid": "0000-0001-8732-0645", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e5fe94feaa4d4ed8929a35838e2c6915.json"}}, {"family": "Eils", "given": "Roland", "initials": "R"}, {"family": "Beaudouin", "given": "Jo\u00ebl", "initials": "J"}, {"family": "\u00d6nfelt", "given": "Bj\u00f6rn", "initials": "B"}, {"family": "Watzl", "given": "Carsten", "initials": "C", "orcid": "0000-0001-5195-0995", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/705dc690627d4843a59d21e3f2b62ea6.json"}}], "type": "journal article", "published": "2019-09-02", "journal": {"title": "J. Exp. Med.", "issn": "1540-9538", "volume": "216", "issue": "9", "pages": "2113-2127", "issn-l": "0022-1007"}, "abstract": "NK cells eliminate virus-infected and tumor cells by releasing cytotoxic granules containing granzyme B (GrzB) or by engaging death receptors that initiate caspase cascades. The orchestrated interplay between both cell death pathways remains poorly defined. Here we simultaneously measure the activities of GrzB and caspase-8 in tumor cells upon contact with human NK cells. We observed that NK cells switch from inducing a fast GrzB-mediated cell death in their first killing events to a slow death receptor-mediated killing during subsequent tumor cell encounters. Target cell contact reduced intracellular GrzB and perforin and increased surface-CD95L in NK cells over time, showing how the switch in cytotoxicity pathways is controlled. Without perforin, NK cells were unable to perform GrzB-mediated serial killing and only killed once via death receptors. In contrast, the absence of CD95 on tumor targets did not impair GrzB-mediated serial killing. This demonstrates that GrzB and death receptor-mediated cytotoxicity are differentially regulated during NK cell serial killing.", "doi": "10.1084/jem.20181454", "pmid": "31270246", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC6719417"}, {"db": "pii", "key": "jem.20181454"}], "notes": [], "created": "2026-08-20T09:38:15.463Z", "modified": "2026-08-20T09:38:15.563Z"}]}