{"entity": "researcher", "timestamp": "2026-08-23T11:06:37.164Z", "family": "Briggs", "given": "John A G", "initials": "JAG", "orcid": "0000-0003-3990-6910", "affiliations": ["MRC Laboratory of Molecular Biology, Cambridge, UK. jbriggs@mrc-lmb.cam.ac.uk."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/702ee550a75346c7ac1cb4832bcf3aef.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/702ee550a75346c7ac1cb4832bcf3aef"}}, "publications": [{"entity": "publication", "iuid": "7d93482253c74fbf98cfde74f2b2d4ae", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/7d93482253c74fbf98cfde74f2b2d4ae.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/7d93482253c74fbf98cfde74f2b2d4ae"}}, "title": "Structural basis for VPS34 kinase activation by Rab1 and Rab5 on membranes.", "authors": [{"family": "Tremel", "given": "Shirley", "initials": "S", "orcid": "0000-0002-4077-0021", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/62440c46afba4e9a80eaefb5ef657680.json"}}, {"family": "Ohashi", "given": "Yohei", "initials": "Y", "orcid": "0000-0002-2288-130X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ff06a49ebb7e41099b458a428bf3fa76.json"}}, {"family": "Morado", "given": "Dustin R", "initials": "DR"}, {"family": "Bertram", "given": "Jessie", "initials": "J"}, {"family": "Perisic", "given": "Olga", "initials": "O", "orcid": "0000-0002-3842-2896", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/cddaff861d4d4280820ac0aebdbeaef0.json"}}, {"family": "Brandt", "given": "Laura T L", "initials": "LTL", "orcid": "0000-0003-1830-4128", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d804015aba8d4211bfe4b5e3b9c9e1d2.json"}}, {"family": "von Wrisberg", "given": "Marie-Kristin", "initials": "MK", "orcid": "0000-0002-7100-4565", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/403af701a74c405aa354146ab3e58ab9.json"}}, {"family": "Chen", "given": "Zhuo A", "initials": "ZA", "orcid": "0000-0003-0811-8348", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/39014f26ee5049649ac404fc8e15a550.json"}}, {"family": "Maslen", "given": "Sarah L", "initials": "SL", "orcid": "0000-0002-0261-2866", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9c9be103e68c4f619db8bfe162175c5a.json"}}, {"family": "Kovtun", "given": "Oleksiy", "initials": "O", "orcid": "0000-0001-6374-7863", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4ead6bd37aeb4c2b8716101956e17ae4.json"}}, {"family": "Skehel", "given": "Mark", "initials": "M", "orcid": "0000-0002-2432-0901", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0a5bf7dfb8e64258a4136260a1a9846a.json"}}, {"family": "Rappsilber", "given": "Juri", "initials": "J"}, {"family": "Lang", "given": "Kathrin", "initials": "K", "orcid": "0000-0002-1318-6567", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f7c04dc7c380470498ed008955868b97.json"}}, {"family": "Munro", "given": "Sean", "initials": "S", "orcid": "0000-0001-6160-5773", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/323cf9816130476d9c14df154b6ec6d8.json"}}, {"family": "Briggs", "given": "John A G", "initials": "JAG", "orcid": "0000-0003-3990-6910", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/702ee550a75346c7ac1cb4832bcf3aef.json"}}, {"family": "Williams", "given": "Roger L", "initials": "RL", "orcid": "0000-0001-7754-4207", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/32df58169da44be593c49b72db3889ae.json"}}], "type": "journal article", "published": "2021-03-10", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "12", "issue": "1", "pages": "1564", "issn-l": "2041-1723"}, "abstract": "The lipid phosphatidylinositol-3-phosphate (PI3P) is a regulator of two fundamental but distinct cellular processes, endocytosis and autophagy, so its generation needs to be under precise temporal and spatial control. PI3P is generated by two complexes that both contain the lipid kinase VPS34: complex II on endosomes (VPS34/VPS15/Beclin 1/UVRAG), and complex I on autophagosomes (VPS34/VPS15/Beclin 1/ATG14L). The endosomal GTPase Rab5 binds complex II, but the mechanism of VPS34 activation by Rab5 has remained elusive, and no GTPase is known to bind complex I. Here we show that Rab5a-GTP recruits endocytic complex II to membranes and activates it by binding between the VPS34 C2 and VPS15 WD40 domains. Electron cryotomography of complex II on Rab5a-decorated vesicles shows that the VPS34 kinase domain is released from inhibition by VPS15 and hovers over the lipid bilayer, poised for catalysis. We also show that the GTPase Rab1a, which is known to be involved in autophagy, recruits and activates the autophagy-specific complex I, but not complex II. Both Rabs bind to the same VPS34 interface but in a manner unique for each. These findings reveal how VPS34 complexes are activated on membranes by specific Rab GTPases and how they are recruited to unique cellular locations.", "doi": "10.1038/s41467-021-21695-2", "pmid": "33692360", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC7946940"}, {"db": "pii", "key": "10.1038/s41467-021-21695-2"}], "notes": [], "created": "2026-08-21T11:48:56.929Z", "modified": "2026-08-21T11:48:57.585Z"}, {"entity": "publication", "iuid": "d7062827fb1743bb860bc7b0c23bce55", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/d7062827fb1743bb860bc7b0c23bce55.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/d7062827fb1743bb860bc7b0c23bce55"}}, "title": "Architecture and mechanism of metazoan retromer:SNX3 tubular coat assembly.", "authors": [{"family": "Leneva", "given": "Natalya", "initials": "N", "orcid": "0000-0001-8102-9769", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5604a5ddf7c64749a52558765a272732.json"}}, {"family": "Kovtun", "given": "Oleksiy", "initials": "O", "orcid": "0000-0001-6374-7863", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4ead6bd37aeb4c2b8716101956e17ae4.json"}}, {"family": "Morado", "given": "Dustin R", "initials": "DR", "orcid": "0000-0003-4693-3220", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0afbe6534a6d4f44996fe626924ec1a9.json"}}, {"family": "Briggs", "given": "John A G", "initials": "JAG", "orcid": "0000-0003-3990-6910", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/702ee550a75346c7ac1cb4832bcf3aef.json"}}, {"family": "Owen", "given": "David J", "initials": "DJ", "orcid": "0000-0002-8351-6322", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b1d71f3ab9db4bdcbd1fa8c6df563377.json"}}], "type": "journal article", "published": "2021-03-00", "journal": {"title": "Sci Adv", "issn": "2375-2548", "volume": "7", "issue": "13", "issn-l": "2375-2548"}, "abstract": "Retromer is a master regulator of cargo retrieval from endosomes, which is critical for many cellular processes including signaling, immunity, neuroprotection, and virus infection. The retromer core (VPS26/VPS29/VPS35) is present on cargo-transporting, tubular carriers along with a range of sorting nexins. Here, we elucidate the structural basis of membrane tubulation and coupled cargo recognition by metazoan and fungal retromer coats assembled with the non-Bin1/Amphiphysin/Rvs (BAR) sorting nexin SNX3 using cryo-electron tomography. The retromer core retains its arched, scaffolding structure but changes its mode of membrane recruitment when assembled with different SNX adaptors, allowing cargo recognition at subunit interfaces. Thus, membrane bending and cargo incorporation can be modulated to allow retromer to traffic cargoes along different cellular transport routes.", "doi": "10.1126/sciadv.abf8598", "pmid": "33762348", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC7990337"}, {"db": "pii", "key": "7/13/eabf8598"}], "notes": [], "created": "2026-08-21T12:22:16.740Z", "modified": "2026-08-21T12:22:16.857Z"}]}