{"entity": "researcher", "timestamp": "2026-08-20T20:50:47.320Z", "family": "\u00d6nfelt", "given": "Bj\u00f6rn", "initials": "B", "orcid": "0000-0001-5178-7593", "affiliations": ["Department of Applied Physics Science for Life Laboratory KTH Royal Institute of Technology  10691 Stockholm Sweden", "Department of Microbiology, Tumor and Cell Biology Karolinska Institute  17177 Stockholm Sweden"], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/6b4b8fcbedb54e2ba38633687f417ae8.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/6b4b8fcbedb54e2ba38633687f417ae8"}}, "publications": [{"entity": "publication", "iuid": "ab9e5d87208f4b86aba55660696ae7c2", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/ab9e5d87208f4b86aba55660696ae7c2.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/ab9e5d87208f4b86aba55660696ae7c2"}}, "title": "A thermoplastic chip for correlative assays combining screening and high-resolution imaging of immune cell responses", "authors": [{"family": "van Ooijen", "given": "Hanna", "initials": "H"}, {"family": "Verron", "given": "Quentin", "initials": "Q"}, {"family": "Zhang", "given": "Hanqing", "initials": "H"}, {"family": "Sandoz", "given": "Patrick A", "initials": "PA"}, {"family": "Frisk", "given": "Thomas W", "initials": "TW"}, {"family": "Carannante", "given": "Valentina", "initials": "V"}, {"family": "Olofsson", "given": "Karl", "initials": "K"}, {"family": "Wagner", "given": "Arnika K", "initials": "AK"}, {"family": "Sandstr\u00f6m", "given": "Niklas", "initials": "N"}, {"family": "\u00d6nfelt", "given": "Bj\u00f6rn", "initials": "B", "orcid": "0000-0001-5178-7593", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6b4b8fcbedb54e2ba38633687f417ae8.json"}}], "type": "posted-content", "published": "2024-10-03", "journal": {"issn-l": null}, "abstract": null, "doi": "10.1101/2024.10.02.616277", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T10:58:44.039Z", "modified": "2026-08-20T10:58:44.089Z"}, {"entity": "publication", "iuid": "6b680093a3cb49cda0c9a98b06504990", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/6b680093a3cb49cda0c9a98b06504990.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/6b680093a3cb49cda0c9a98b06504990"}}, "title": "Direct presentation of inflammation-associated self-antigens by thymic innate-like T cells induces elimination of autoreactive CD8+ thymocytes.", "authors": [{"family": "You", "given": "Yuanyuan", "initials": "Y"}, {"family": "Dunst", "given": "Josefine", "initials": "J"}, {"family": "Ye", "given": "Kewei", "initials": "K"}, {"family": "Sandoz", "given": "Patrick A", "initials": "PA", "orcid": "0000-0002-8379-7267", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d309ec0cb74f41a49a18e226079e6787.json"}}, {"family": "Reinhardt", "given": "Annika", "initials": "A"}, {"family": "Sandrock", "given": "Inga", "initials": "I"}, {"family": "Comet", "given": "Natalia R", "initials": "NR"}, {"family": "Sarkar", "given": "Rupak Dey", "initials": "RD", "orcid": "0009-0008-0203-5772", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/24869b9044774be6bde6090a2e2c27ed.json"}}, {"family": "Yang", "given": "Emily", "initials": "E", "orcid": "0000-0002-6028-2455", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d94a4017752b450c96df61ec726a0613.json"}}, {"family": "Duprez", "given": "Estelle", "initials": "E", "orcid": "0000-0003-3810-5740", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/2b3b8efc080f49d8b3e02bf5a07b2409.json"}}, {"family": "Agudo", "given": "Judith", "initials": "J"}, {"family": "Brown", "given": "Brian D", "initials": "BD", "orcid": "0000-0002-3670-8778", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b871795458dc4bd8836af26e853fb34f.json"}}, {"family": "Utz", "given": "Paul J", "initials": "PJ", "orcid": "0000-0003-1181-1565", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/7d6ff897a183439d8895f72eb18cfdb3.json"}}, {"family": "Kastenm\u00fcller", "given": "Wolfgang", "initials": "W", "orcid": "0000-0002-3835-1485", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5be40dc506934b74bc74acf292df18e2.json"}}, {"family": "Gerlach", "given": "Carmen", "initials": "C", "orcid": "0000-0001-7889-2393", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8053f26c59c24fe9b98ef54a2ba1b16b.json"}}, {"family": "Prinz", "given": "Immo", "initials": "I"}, {"family": "\u00d6nfelt", "given": "Bj\u00f6rn", "initials": "B", "orcid": "0000-0001-5178-7593", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6b4b8fcbedb54e2ba38633687f417ae8.json"}}, {"family": "Kreslavsky", "given": "Taras", "initials": "T", "orcid": "0000-0002-6672-1914", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a81b55340f7c4eda9b079c0ddc182a6d.json"}}], "type": "journal article", "published": "2024-08-00", "journal": {"title": "Nat. Immunol.", "issn": "1529-2916", "volume": "25", "issue": "8", "pages": "1367-1382", "issn-l": "1529-2908"}, "abstract": "Upregulation of diverse self-antigens that constitute components of the inflammatory response overlaps spatially and temporally with the emergence of pathogen-derived foreign antigens. Therefore, discrimination between these inflammation-associated self-antigens and pathogen-derived molecules represents a unique challenge for the adaptive immune system. Here, we demonstrate that CD8+ T cell tolerance to T cell-derived inflammation-associated self-antigens is efficiently induced in the thymus and supported by redundancy in cell types expressing these molecules. In addition to thymic epithelial cells, this included thymic eosinophils and innate-like T cells, a population that expressed molecules characteristic for all major activated T cell subsets. We show that direct T cell-to-T cell antigen presentation by minute numbers of innate-like T cells was sufficient to eliminate autoreactive CD8+ thymocytes. Tolerance to such effector molecules was of critical importance, as its breach caused by decreased thymic abundance of a single model inflammation-associated self-antigen resulted in autoimmune elimination of an entire class of effector T cells.", "doi": "10.1038/s41590-024-01899-6", "pmid": "38992254", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC11291280"}, {"db": "pii", "key": "10.1038/s41590-024-01899-6"}], "notes": [], "created": "2026-08-20T09:01:42.380Z", "modified": "2026-08-20T09:01:42.758Z"}, {"entity": "publication", "iuid": "2e468fb6ee84403d9cddc6c5ef0a7713", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/2e468fb6ee84403d9cddc6c5ef0a7713.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/2e468fb6ee84403d9cddc6c5ef0a7713"}}, "title": "Modulation of lytic molecules restrain serial killing in \u03b3\u03b4 T lymphocytes.", "authors": [{"family": "Sandoz", "given": "Patrick A", "initials": "PA", "orcid": "0000-0002-8379-7267", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d309ec0cb74f41a49a18e226079e6787.json"}}, {"family": "Kuhnigk", "given": "Kyra", "initials": "K", "orcid": "0000-0002-2265-8204", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c9038f497daf47f4b001942f2d6a5788.json"}}, {"family": "Szabo", "given": "Edina K", "initials": "EK"}, {"family": "Thunberg", "given": "Sarah", "initials": "S", "orcid": "0000-0001-6912-6048", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4c1a27ca8cd2429098eeb6bbebaa73f5.json"}}, {"family": "Erikson", "given": "Elina", "initials": "E"}, {"family": "Sandstr\u00f6m", "given": "Niklas", "initials": "N", "orcid": "0000-0001-6443-878X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b65278c9896e466f92f62f3ba1e58136.json"}}, {"family": "Verron", "given": "Quentin", "initials": "Q", "orcid": "0000-0001-5800-4379", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/31de7b29ad984949bc1901417bc7ed25.json"}}, {"family": "Brech", "given": "Andreas", "initials": "A"}, {"family": "Watzl", "given": "Carsten", "initials": "C", "orcid": "0000-0001-5195-0995", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/705dc690627d4843a59d21e3f2b62ea6.json"}}, {"family": "Wagner", "given": "Arnika K", "initials": "AK"}, {"family": "Alici", "given": "Evren", "initials": "E"}, {"family": "Malmberg", "given": "Karl-Johan", "initials": "KJ", "orcid": "0000-0002-8718-9373", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e1b891c497ca402aaff79e8082f22553.json"}}, {"family": "Uhlin", "given": "Michael", "initials": "M", "orcid": "0000-0002-2177-6727", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/116cfe482bb941159ee2e7175f1cd095.json"}}, {"family": "\u00d6nfelt", "given": "Bj\u00f6rn", "initials": "B", "orcid": "0000-0001-5178-7593", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6b4b8fcbedb54e2ba38633687f417ae8.json"}}], "type": "journal article", "published": "2023-09-27", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "14", "issue": "1", "pages": "6035", "issn-l": "2041-1723"}, "abstract": "\u03b3\u03b4 T cells play a pivotal role in protection against various types of infections and tumours, from early childhood on and throughout life. They consist of several subsets characterised by adaptive and innate-like functions, with V\u03b39V\u03b42 being the largest subset in human peripheral blood. Although these cells show signs of cytotoxicity, their modus operandi remains poorly understood. Here we explore, using live single-cell imaging, the cytotoxic functions of \u03b3\u03b4 T cells upon interactions with tumour target cells with high temporal and spatial resolution. While \u03b3\u03b4 T cell killing is dominated by degranulation, the availability of lytic molecules appears tightly regulated in time and space. In particular, the limited co-occurrence of granzyme B and perforin restrains serial killing of tumour cells by \u03b3\u03b4 T cells. Thus, our data provide new insights into the cytotoxic arsenal and functions of \u03b3\u03b4 T cells, which may guide the development of more efficient \u03b3\u03b4 T cell based adoptive immunotherapies.", "doi": "10.1038/s41467-023-41634-7", "pmid": "37758698", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC10533871"}, {"db": "pii", "key": "10.1038/s41467-023-41634-7"}], "notes": [], "created": "2026-08-20T08:52:55.661Z", "modified": "2026-08-20T08:52:56.006Z"}, {"entity": "publication", "iuid": "9551a22f052a4e8eb02f43df52a2288c", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/9551a22f052a4e8eb02f43df52a2288c.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/9551a22f052a4e8eb02f43df52a2288c"}}, "title": "Modulation of lytic molecules restrain serial killing in \u03b3\u03b4 T lymphocytes", "authors": [{"family": "Onfelt", "given": "Bjorn", "initials": "B", "orcid": "0000-0001-5178-7593", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6b4b8fcbedb54e2ba38633687f417ae8.json"}}, {"family": "Sandoz", "given": "Patrick", "initials": "P"}, {"family": "Kuhnigk", "given": "Kyra", "initials": "K"}, {"family": "Szabo", "given": "Edina", "initials": "E"}, {"family": "Thunberg", "given": "Sarah", "initials": "S", "orcid": "0000-0001-6912-6048", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4c1a27ca8cd2429098eeb6bbebaa73f5.json"}}, {"family": "Erikson", "given": "Elina", "initials": "E"}, {"family": "Sandstr\u00f6m", "given": "Niklas", "initials": "N", "orcid": "0000-0001-6443-878X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b65278c9896e466f92f62f3ba1e58136.json"}}, {"family": "Verron", "given": "Quentin", "initials": "Q"}, {"family": "Brech", "given": "Andreas", "initials": "A"}, {"family": "Watzl", "given": "Carsten", "initials": "C", "orcid": "0000-0001-5195-0995", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/705dc690627d4843a59d21e3f2b62ea6.json"}}, {"family": "Wagner", "given": "Arnika", "initials": "A"}, {"family": "Alici", "given": "Evren", "initials": "E"}, {"family": "Malmberg", "given": "Karl Johan", "initials": "KJ", "orcid": "0000-0002-8718-9373", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e1b891c497ca402aaff79e8082f22553.json"}}, {"family": "Uhlin", "given": "Michael", "initials": "M", "orcid": "0000-0002-2177-6727", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/116cfe482bb941159ee2e7175f1cd095.json"}}], "type": "posted-content", "published": "2023-02-21", "journal": {"issn-l": null}, "abstract": null, "doi": "10.21203/rs.3.rs-2371463/v1", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T12:58:48.009Z", "modified": "2026-08-20T12:58:48.055Z"}, {"entity": "publication", "iuid": "93affd71ae484063aa0e659fb984e380", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/93affd71ae484063aa0e659fb984e380.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/93affd71ae484063aa0e659fb984e380"}}, "title": "Live single cell imaging assays in glass microwells produced by laser-induced deep etching.", "authors": [{"family": "Sandstr\u00f6m", "given": "Niklas", "initials": "N", "orcid": "0000-0001-6443-878X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b65278c9896e466f92f62f3ba1e58136.json"}}, {"family": "Brandt", "given": "Ludwig", "initials": "L", "orcid": "0000-0003-2040-3176", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/328e28faa0794e95a4e6cd96e2508ad0.json"}}, {"family": "Sandoz", "given": "Patrick A", "initials": "PA", "orcid": "0000-0002-8379-7267", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d309ec0cb74f41a49a18e226079e6787.json"}}, {"family": "Zambarda", "given": "Chiara", "initials": "C"}, {"family": "Guldevall", "given": "Karolin", "initials": "K"}, {"family": "Schulz-Ruhtenberg", "given": "Malte", "initials": "M"}, {"family": "R\u00f6sener", "given": "Bernd", "initials": "B"}, {"family": "Kr\u00fcger", "given": "Robin A", "initials": "RA"}, {"family": "\u00d6nfelt", "given": "Bj\u00f6rn", "initials": "B", "orcid": "0000-0001-5178-7593", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6b4b8fcbedb54e2ba38633687f417ae8.json"}}], "type": "journal article", "published": "2022-05-31", "journal": {"title": "Lab Chip", "issn": "1473-0189", "volume": "22", "issue": "11", "pages": "2107-2121", "issn-l": null}, "abstract": "Miniaturization of cell culture substrates enables controlled analysis of living cells in confined micro-scale environments. This is particularly suitable for imaging individual cells over time, as they can be monitored without escaping the imaging field-of-view (FoV). Glass materials are ideal for most microscopy applications. However, with current methods used in life sciences, glass microfabrication is limited in terms of either freedom of design, quality, or throughput. In this work, we introduce laser-induced deep etching (LIDE) as a method for producing glass microwell arrays for live single cell imaging assays. We demonstrate novel microwell arrays with deep, high-aspect ratio wells that have rounded, dimpled or flat bottom profiles in either single-layer or double-layer glass chips. The microwells are evaluated for microscopy-based analysis of long-term cell culture, clonal expansion, laterally organized cell seeding, subcellular mechanics during migration and immune cell cytotoxicity assays of both adherent and suspension cells. It is shown that all types of microwells can support viable cell cultures and imaging with single cell resolution, and we highlight specific benefits of each microwell design for different applications. We believe that high-quality glass microwell arrays enabled by LIDE provide a great option for high-content and high-resolution imaging-based live cell assays with a broad range of potential applications within life sciences.", "doi": "10.1039/d2lc00090c", "pmid": "35470832", "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T09:27:30.995Z", "modified": "2026-08-20T09:27:31.118Z"}, {"entity": "publication", "iuid": "6087ac4b304c4b82a815ab2e05edcc5b", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/6087ac4b304c4b82a815ab2e05edcc5b.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/6087ac4b304c4b82a815ab2e05edcc5b"}}, "title": "Correction to: IL\u201115 superagonist N\u2011803 improves IFN\u03b3 production and killing of leukemia and ovarian cancer cells by CD34+ progenitor\u2011derived NK cells.", "authors": [{"family": "Van der Meer", "given": "J M R", "initials": "JMR", "orcid": "0000-0001-9288-1500", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ec374420f13d480ba3e342acba183a7c.json"}}, {"family": "Maas", "given": "R J A", "initials": "RJA", "orcid": "0000-0002-3185-5226", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a4de8f2b519342279c7578490873c4a6.json"}}, {"family": "Guldevall", "given": "K", "initials": "K", "orcid": "0000-0003-1016-2460", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/24a750602b1a47d48d92974f972f1c72.json"}}, {"family": "Klarenaar", "given": "K", "initials": "K"}, {"family": "De Jonge", "given": "P K J D", "initials": "PKJD"}, {"family": "Hoogstad-van Evert", "given": "J S", "initials": "JS", "orcid": "0000-0001-9838-0977", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9c4e67260bf64a2d963820d64cd4b162.json"}}, {"family": "van der Waart", "given": "A B", "initials": "AB", "orcid": "0000-0001-5986-3060", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8a4ab943bebb4a518668d1f1e9dd7363.json"}}, {"family": "Cany", "given": "J", "initials": "J", "orcid": "0000-0002-5214-639X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a526e4e6cde24c6c8807b27101e35ea3.json"}}, {"family": "Safrit", "given": "J T", "initials": "JT", "orcid": "0000-0001-7441-4423", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/fb8265befd554a1aba34b695852a1016.json"}}, {"family": "Lee", "given": "J H", "initials": "JH"}, {"family": "Wagena", "given": "E", "initials": "E"}, {"family": "Friedl", "given": "P", "initials": "P", "orcid": "0000-0002-0119-4041", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5d4fb98a43c1442f9cd904e323dc2769.json"}}, {"family": "\u00d6nfelt", "given": "B", "initials": "B", "orcid": "0000-0001-5178-7593", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6b4b8fcbedb54e2ba38633687f417ae8.json"}}, {"family": "Massuger", "given": "L F", "initials": "LF", "orcid": "0000-0002-1539-8985", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/cd1dab07b61e43b29f9bcdda0383ed00.json"}}, {"family": "Schaap", "given": "N P M", "initials": "NPM", "orcid": "0000-0001-7696-4752", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/2e6c952987b149e1b97baab1b815b600.json"}}, {"family": "Jansen", "given": "J H", "initials": "JH", "orcid": "0000-0001-9459-568X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f4dda0ce64c04555834f61ba0d31278d.json"}}, {"family": "Hobo", "given": "W", "initials": "W", "orcid": "0000-0002-8206-8185", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3d9d1653bbb04e1cb8ec62f49217057d.json"}}, {"family": "Dolstra", "given": "H", "initials": "H", "orcid": "0000-0002-3998-687X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a45d0970dc374c218087801fef1baa2a.json"}}], "type": "published erratum", "published": "2021-11-00", "journal": {"title": "Cancer Immunol. Immunother.", "issn": "1432-0851", "volume": "70", "issue": "11", "pages": "3367", "issn-l": "0340-7004"}, "abstract": null, "doi": "10.1007/s00262-021-03049-5", "pmid": "34524494", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC8505330"}, {"db": "pii", "key": "10.1007/s00262-021-03049-5"}], "notes": [], "created": "2026-08-20T06:39:30.255Z", "modified": "2026-08-20T06:39:30.394Z"}, {"entity": "publication", "iuid": "960e80f94c714e4d9469b1e3e2d8d29d", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/960e80f94c714e4d9469b1e3e2d8d29d.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/960e80f94c714e4d9469b1e3e2d8d29d"}}, "title": "IL-15 superagonist N-803 improves IFN\u03b3 production and killing of leukemia and ovarian cancer cells by CD34+ progenitor-derived NK cells.", "authors": [{"family": "Van der Meer", "given": "J M R", "initials": "JMR", "orcid": "0000-0001-9288-1500", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ec374420f13d480ba3e342acba183a7c.json"}}, {"family": "Maas", "given": "R J A", "initials": "RJA", "orcid": "0000-0002-3185-5226", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a4de8f2b519342279c7578490873c4a6.json"}}, {"family": "Guldevall", "given": "K", "initials": "K", "orcid": "0000-0003-1016-2460", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/24a750602b1a47d48d92974f972f1c72.json"}}, {"family": "Klarenaar", "given": "K", "initials": "K"}, {"family": "de Jonge", "given": "P K J D", "initials": "PKJD"}, {"family": "Evert", "given": "J S Hoogstad-van", "initials": "JSH", "orcid": "0000-0001-9838-0977", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9c4e67260bf64a2d963820d64cd4b162.json"}}, {"family": "van der Waart", "given": "A B", "initials": "AB", "orcid": "0000-0001-5986-3060", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8a4ab943bebb4a518668d1f1e9dd7363.json"}}, {"family": "Cany", "given": "J", "initials": "J", "orcid": "0000-0002-5214-639X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a526e4e6cde24c6c8807b27101e35ea3.json"}}, {"family": "Safrit", "given": "J T", "initials": "JT", "orcid": "0000-0001-7441-4423", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/fb8265befd554a1aba34b695852a1016.json"}}, {"family": "Lee", "given": "J H", "initials": "JH"}, {"family": "Wagena", "given": "E", "initials": "E"}, {"family": "Friedl", "given": "P", "initials": "P", "orcid": "0000-0002-0119-4041", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5d4fb98a43c1442f9cd904e323dc2769.json"}}, {"family": "\u00d6nfelt", "given": "B", "initials": "B", "orcid": "0000-0001-5178-7593", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6b4b8fcbedb54e2ba38633687f417ae8.json"}}, {"family": "Massuger", "given": "L F", "initials": "LF", "orcid": "0000-0002-1539-8985", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/cd1dab07b61e43b29f9bcdda0383ed00.json"}}, {"family": "Schaap", "given": "N P M", "initials": "NPM", "orcid": "0000-0001-7696-4752", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/2e6c952987b149e1b97baab1b815b600.json"}}, {"family": "Jansen", "given": "J H", "initials": "JH", "orcid": "0000-0001-9459-568X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f4dda0ce64c04555834f61ba0d31278d.json"}}, {"family": "Hobo", "given": "W", "initials": "W", "orcid": "0000-0002-8206-8185", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3d9d1653bbb04e1cb8ec62f49217057d.json"}}, {"family": "Dolstra", "given": "H", "initials": "H", "orcid": "0000-0002-3998-687X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a45d0970dc374c218087801fef1baa2a.json"}}], "type": "journal article", "published": "2021-05-00", "journal": {"title": "Cancer Immunol. Immunother.", "issn": "1432-0851", "volume": "70", "issue": "5", "pages": "1305-1321", "issn-l": "0340-7004"}, "abstract": "Allogeneic natural killer (NK) cell transfer is a potential immunotherapy to eliminate and control cancer. A promising source are CD34 + hematopoietic progenitor cells (HPCs), since large numbers of cytotoxic NK cells can be generated. Effective boosting of NK cell function can be achieved by interleukin (IL)-15. However, its in vivo half-life is short and potent trans-presentation by IL-15 receptor \u03b1 (IL-15R\u03b1) is absent. Therefore, ImmunityBio developed IL-15 superagonist N-803, which combines IL-15 with an activating mutation, an IL-15R\u03b1 sushi domain for trans-presentation, and IgG1-Fc for increased half-life. Here, we investigated whether and how N-803 improves HPC-NK cell functionality in leukemia and ovarian cancer (OC) models in vitro and in vivo in OC-bearing immunodeficient mice. We used flow cytometry-based assays, enzyme-linked immunosorbent assay, microscopy-based serial killing assays, and bioluminescence imaging, for in vitro and in vivo experiments. N-803 increased HPC-NK cell proliferation and interferon (IFN)\u03b3 production. On leukemia cells, co-culture with HPC-NK cells and N-803 increased ICAM-1 expression. Furthermore, N-803 improved HPC-NK cell-mediated (serial) leukemia killing. Treating OC spheroids with HPC-NK cells and N-803 increased IFN\u03b3-induced CXCL10 secretion, and target killing after prolonged exposure. In immunodeficient mice bearing human OC, N-803 supported HPC-NK cell persistence in combination with total human immunoglobulins to prevent Fc-mediated HPC-NK cell depletion. Moreover, this combination treatment decreased tumor growth. In conclusion, N-803 is a promising IL-15-based compound that boosts HPC-NK cell expansion and functionality in vitro and in vivo. Adding N-803 to HPC-NK cell therapy could improve cancer immunotherapy.", "doi": "10.1007/s00262-020-02749-8", "pmid": "33140189", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC8053152"}, {"db": "pii", "key": "10.1007/s00262-020-02749-8"}], "notes": [], "created": "2026-08-20T06:39:27.597Z", "modified": "2026-08-20T06:39:28.213Z"}, {"entity": "publication", "iuid": "aca3f052271f4513a262eec06daa0c47", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/aca3f052271f4513a262eec06daa0c47.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/aca3f052271f4513a262eec06daa0c47"}}, "title": "A Fluorescent Kinase Inhibitor that Exhibits Diagnostic Changes in Emission upon Binding.", "authors": [{"family": "Fleming", "given": "Cassandra L", "initials": "CL", "orcid": "0000-0002-7730-7305", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/06cf797b8d614373823d7ea82201f6e8.json"}}, {"family": "Sandoz", "given": "Patrick A", "initials": "PA", "orcid": "0000-0002-8379-7267", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d309ec0cb74f41a49a18e226079e6787.json"}}, {"family": "Inghardt", "given": "Tord", "initials": "T", "orcid": "0000-0002-4804-9474", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/abf1747bec314656809ff2e84ed9aa69.json"}}, {"family": "\u00d6nfelt", "given": "Bj\u00f6rn", "initials": "B", "orcid": "0000-0001-5178-7593", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6b4b8fcbedb54e2ba38633687f417ae8.json"}}, {"family": "Gr\u00f8tli", "given": "Morten", "initials": "M", "orcid": "0000-0003-3621-4222", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f3d8708707ca48078f8113ecdeb9e6cb.json"}}, {"family": "Andr\u00e9asson", "given": "Joakim", "initials": "J", "orcid": "0000-0003-4695-7943", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/41692252926d40fd883c3fe48fa8cd07.json"}}], "type": "journal article", "published": "2019-10-14", "journal": {"title": "Angew. Chem. Int. Ed. Engl.", "issn": "1521-3773", "volume": "58", "issue": "42", "pages": "15000-15004", "issn-l": "1433-7851"}, "abstract": "The development of a fluorescent LCK inhibitor that exhibits favourable solvatochromic properties upon binding the kinase is described. Fluorescent properties were realised through the inclusion of a prodan-derived fluorophore into the pharmacophore of an ATP-competitive kinase inhibitor. Fluorescence titration experiments demonstrate the solvatochromic properties of the inhibitor, in which dramatic increase in emission intensity and hypsochromic shift in emission maxima are clearly observed upon binding LCK. Microscopy experiments in cellular contexts together with flow cytometry show that the fluorescence intensity of the inhibitor correlates with the LCK concentration. Furthermore, multiphoton microscopy experiments demonstrate both the rapid cellular uptake of the inhibitor and that the two-photon cross section of the inhibitor is amenable for excitation at 700 nm.", "doi": "10.1002/anie.201909536", "pmid": "31411364", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC6851755"}], "notes": [], "created": "2026-08-20T06:29:55.975Z", "modified": "2026-08-20T06:29:56.055Z"}, {"entity": "publication", "iuid": "de3a67d6c35b4ee2847244e886fa06cd", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/de3a67d6c35b4ee2847244e886fa06cd.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/de3a67d6c35b4ee2847244e886fa06cd"}}, "title": "A Fluorescent Kinase Inhibitor that Exhibits Diagnostic Changes in Emission upon Binding", "authors": [{"family": "Fleming", "given": "Cassandra L", "initials": "CL", "orcid": "0000-0002-7730-7305", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/06cf797b8d614373823d7ea82201f6e8.json"}}, {"family": "Sandoz", "given": "Patrick A", "initials": "PA", "orcid": "0000-0002-8379-7267", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d309ec0cb74f41a49a18e226079e6787.json"}}, {"family": "Inghardt", "given": "Tord", "initials": "T", "orcid": "0000-0002-4804-9474", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/abf1747bec314656809ff2e84ed9aa69.json"}}, {"family": "\u00d6nfelt", "given": "Bj\u00f6rn", "initials": "B", "orcid": "0000-0001-5178-7593", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6b4b8fcbedb54e2ba38633687f417ae8.json"}}, {"family": "Gr\u00f8tli", "given": "Morten", "initials": "M", "orcid": "0000-0003-3621-4222", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f3d8708707ca48078f8113ecdeb9e6cb.json"}}, {"family": "Andr\u00e9asson", "given": "Joakim", "initials": "J", "orcid": "0000-0003-4695-7943", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/41692252926d40fd883c3fe48fa8cd07.json"}}], "type": "journal-article", "published": "2019-10-14", "journal": {"title": "Angewandte Chemie", "issn": "0044-8249", "volume": "131", "issue": "42", "pages": "15142-15146", "issn-l": null}, "abstract": null, "doi": "10.1002/ange.201909536", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T06:29:32.914Z", "modified": "2026-08-20T06:29:33.126Z"}]}