{"entity": "researcher", "timestamp": "2026-08-23T22:15:57.018Z", "family": "Lind", "given": "Sara Bergstr\u00f6m", "initials": "SB", "orcid": "0000-0002-9510-3816", "affiliations": ["Section of Analytical Chemistry, Department of Chemistry-BMC, Uppsala University, Uppsala, 751 24, Sweden."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/678c39bf7867429495690a7c82fcb6f7.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/678c39bf7867429495690a7c82fcb6f7"}}, "publications": [{"entity": "publication", "iuid": "ab6fae7031264c94b78e6c53fbe8fc5c", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/ab6fae7031264c94b78e6c53fbe8fc5c.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/ab6fae7031264c94b78e6c53fbe8fc5c"}}, "title": "Phosphorylation Time-Course Study of the Response during Adenovirus Type 2 Infection.", "authors": [{"family": "Vald\u00e9s", "given": "Alberto", "initials": "A", "orcid": "0000-0002-7901-5816", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ec5243e1be3c4491bfa437deb19183f3.json"}}, {"family": "Zhao", "given": "Hongxing", "initials": "H"}, {"family": "Pettersson", "given": "Ulf", "initials": "U"}, {"family": "Lind", "given": "Sara Bergstr\u00f6m", "initials": "SB", "orcid": "0000-0002-9510-3816", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/678c39bf7867429495690a7c82fcb6f7.json"}}], "type": "journal article", "published": "2020-04-00", "journal": {"title": "Proteomics", "issn": "1615-9861", "volume": "20", "issue": "7", "pages": "e1900327", "issn-l": "1615-9853"}, "abstract": "PTMs such as phosphorylations are usually involved in signal transduction pathways. To investigate the temporal dynamics of phosphoproteome changes upon viral infection, a model system of IMR-90 cells infected with human adenovirus type 2 (Ad2) is used in a time-course quantitative analysis combining titanium dioxide (TiO2 ) particle enrichment and SILAC-MS. Quantitative data from 1552 phosphorylated sites clustered the highly altered phosphorylated sites to the signaling by rho family GTPases, the actin cytoskeleton signaling, and the cAMP-dependent protein kinase A signaling pathways. Their activation is especially pronounced at early time post-infection. Changes of several phosphorylated sites involved in the glycolysis pathway, related to the activation of the Warburg effect, point at virus-induced energy production. For Ad2 proteins, 32 novel phosphorylation sites are identified and as many as 52 phosphorylated sites on 17 different Ad2 proteins are quantified, most of them at late time post-infection. Kinase predictions highlighted activation of PKA, CDK1/2, MAPK, and CKII. Overlaps of kinase motif sequences for viral and human proteins are observed, stressing the importance of phosphorylation during Ad2 infection.", "doi": "10.1002/pmic.201900327", "pmid": "32032466", "labels": [], "xrefs": [], "notes": [], "created": "2026-08-21T11:04:14.952Z", "modified": "2026-08-21T11:04:15.072Z"}, {"entity": "publication", "iuid": "9cdfbc7b5e1c441b9e90241b902d72f7", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/9cdfbc7b5e1c441b9e90241b902d72f7.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/9cdfbc7b5e1c441b9e90241b902d72f7"}}, "title": "Time-resolved proteomics of adenovirus infected cells.", "authors": [{"family": "Vald\u00e9s", "given": "Alberto", "initials": "A"}, {"family": "Zhao", "given": "Hongxing", "initials": "H"}, {"family": "Pettersson", "given": "Ulf", "initials": "U"}, {"family": "Lind", "given": "Sara Bergstr\u00f6m", "initials": "SB", "orcid": "0000-0002-9510-3816", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/678c39bf7867429495690a7c82fcb6f7.json"}}], "type": "journal article", "published": "2018-09-25", "journal": {"title": "PLoS ONE", "issn": "1932-6203", "volume": "13", "issue": "9", "pages": "e0204522", "issn-l": "1932-6203"}, "abstract": "Viral infections cause large problems in the world and deeper understanding of the disease mechanisms is needed. Here we present an analytical strategy to investigate the host cell protein changes during human adenovirus type 2 (HAdV-C2 or Ad2) infection of lung fibroblasts by stable isotope labelling of amino acids in cell culture (SILAC) and nanoLC-MS/MS. This work focuses on early phase of infection (6 and 12 h post-infection (hpi)) but the data is combined with previously published late phase (24 and 36 hpi) proteomics data to produce a time series covering the complete infection. As many as 2169 proteins were quantitatively monitored from 6 to 36 hpi, while some proteins were time-specific. After applying different filter criteria, 2027 and 2150 proteins were quantified at 6 and 12 hpi and among them, 431 and 544 were significantly altered at the two time points. Pathway analysis showed that the De novo purine and pyrimidine biosynthesis, Glycolysis and Cytoskeletal regulation by Rho GTPase pathways were activated early during infection while inactivation of the Integrin signalling pathway started between 6 and 12 hpi. Moreover, upstream regulator analysis predicted MYC to be activated with time of infection and protein and RNA data for genes controlled by this transcription factor showed good correlation, which validated the use of protein data for this prediction. Among the identified phosphorylation sites, a group related to glycolysis and cytoskeletal reorganization were up-regulated during infection. The results show specific aspects on how the host cell proteins, the final products in the genetic information flow, are influenced by Ad2 infection, which would be overlooked if only knowledge derived from mRNA data is considered.", "doi": "10.1371/journal.pone.0204522", "pmid": "30252905", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC6155545"}, {"db": "pii", "key": "PONE-D-18-17619"}], "notes": [], "created": "2026-08-21T12:51:55.901Z", "modified": "2026-08-21T12:51:55.917Z"}]}