{"entity": "researcher", "timestamp": "2026-08-20T20:54:00.670Z", "family": "Stoecklin", "given": "Georg", "initials": "G", "orcid": "0000-0001-9284-9834", "affiliations": ["Division of Biochemistry, Mannheim Institute for Innate Immunoscience (MI3), Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany.", "Center for Molecular Biology of Heidelberg University (ZMBH), German Cancer Research Center (DKFZ), DKFZ-ZMBH Alliance, National Center for Tumor Diseases (NCT), Heidelberg, Germany."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/65fee664b61c42b1ad51358fe34e2608.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/65fee664b61c42b1ad51358fe34e2608"}}, "publications": [{"entity": "publication", "iuid": "a9924f0b5427486390e3b236da64b749", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/a9924f0b5427486390e3b236da64b749.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/a9924f0b5427486390e3b236da64b749"}}, "title": "Widespread displacement of DNA- and RNA-binding factors underlies toxicity of arginine-rich cell-penetrating peptides.", "authors": [{"family": "Lafarga", "given": "Vanesa", "initials": "V"}, {"family": "Sirozh", "given": "Oleksandra", "initials": "O"}, {"family": "D\u00edaz-L\u00f3pez", "given": "Irene", "initials": "I"}, {"family": "Galarreta", "given": "Antonio", "initials": "A", "orcid": "0000-0003-2358-7927", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/afa6e99550224076bf06615778479685.json"}}, {"family": "Hisaoka", "given": "Misaru", "initials": "M"}, {"family": "Zarzuela", "given": "Eduardo", "initials": "E"}, {"family": "Boskovic", "given": "Jasminka", "initials": "J"}, {"family": "Jovanovic", "given": "Bogdan", "initials": "B"}, {"family": "Fernandez-Leiro", "given": "Rafael", "initials": "R", "orcid": "0000-0002-7941-0357", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/082cd4d551754cbd91af9bcf249a3912.json"}}, {"family": "Mu\u00f1oz", "given": "Jaime", "initials": "J"}, {"family": "Stoecklin", "given": "Georg", "initials": "G", "orcid": "0000-0001-9284-9834", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/65fee664b61c42b1ad51358fe34e2608.json"}}, {"family": "Ventoso", "given": "Iv\u00e1n", "initials": "I"}, {"family": "Fernandez-Capetillo", "given": "Oscar", "initials": "O", "orcid": "0000-0002-2690-6885", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ab1f07900e6e466d83763e5fe0020ed6.json"}}], "type": "journal article", "published": "2021-07-01", "journal": {"title": "EMBO J.", "issn": "1460-2075", "volume": "40", "issue": "13", "pages": "e103311", "issn-l": "0261-4189"}, "abstract": "Due to their capability to transport chemicals or proteins into target cells, cell-penetrating peptides (CPPs) are being developed as therapy delivery tools. However, and despite their interesting properties, arginine-rich CPPs often show toxicity for reasons that remain poorly understood. Using a (PR)n dipeptide repeat that has been linked to amyotrophic lateral sclerosis (ALS) as a model of an arginine-rich CPP, we here show that the presence of (PR)n leads to a generalized displacement of RNA- and DNA-binding proteins from chromatin and mRNA. Accordingly, any reaction involving nucleic acids, such as RNA transcription, translation, splicing and degradation, or DNA replication and repair, is impaired by the presence of the CPPs. Interestingly, the effects of (PR)n are fully mimicked by protamine, a small arginine-rich protein that displaces histones from chromatin during spermatogenesis. We propose that widespread coating of nucleic acids and consequent displacement of RNA- and DNA-binding factors from chromatin and mRNA accounts for the toxicity of arginine-rich CPPs, including those that have been recently associated with the onset of ALS.", "doi": "10.15252/embj.2019103311", "pmid": "33978236", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC8246256"}], "notes": [], "created": "2026-08-20T12:51:31.926Z", "modified": "2026-08-20T12:51:32.043Z"}, {"entity": "publication", "iuid": "b140a161ebaf47ebba57dce0120b0cec", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/b140a161ebaf47ebba57dce0120b0cec.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/b140a161ebaf47ebba57dce0120b0cec"}}, "title": "TIAR marks nuclear G2/M transition granules and restricts CDK1 activity under replication stress.", "authors": [{"family": "Lafarga", "given": "Vanesa", "initials": "V"}, {"family": "Sung", "given": "Hsu-Min", "initials": "HM", "orcid": "0000-0001-6498-4254", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/2030f5f31774439da61719b7315858af.json"}}, {"family": "Haneke", "given": "Katharina", "initials": "K"}, {"family": "Roessig", "given": "Lea", "initials": "L"}, {"family": "Pauleau", "given": "Anne-Laure", "initials": "AL"}, {"family": "Bruer", "given": "Marius", "initials": "M"}, {"family": "Rodriguez-Acebes", "given": "Sara", "initials": "S"}, {"family": "Lopez-Contreras", "given": "Andres J", "initials": "AJ"}, {"family": "Gruss", "given": "Oliver J", "initials": "OJ"}, {"family": "Erhardt", "given": "Sylvia", "initials": "S", "orcid": "0000-0003-3169-241X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/75389c685360494fbb76e9737538165c.json"}}, {"family": "Mendez", "given": "Juan", "initials": "J"}, {"family": "Fernandez-Capetillo", "given": "Oscar", "initials": "O"}, {"family": "Stoecklin", "given": "Georg", "initials": "G", "orcid": "0000-0001-9284-9834", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/65fee664b61c42b1ad51358fe34e2608.json"}}], "type": "journal article", "published": "2019-01-00", "journal": {"title": "EMBO Rep.", "issn": "1469-3178", "volume": "20", "issue": "1", "issn-l": "1469-221X"}, "abstract": "The G2/M checkpoint coordinates DNA replication with mitosis and thereby prevents chromosome segregation in the presence of unreplicated or damaged DNA Here, we show that the RNA-binding protein TIAR is essential for the G2/M checkpoint and that TIAR accumulates in nuclear foci in late G2 and prophase in cells suffering from replication stress. These foci, which we named G2/M transition granules (GMGs), occur at low levels in normally cycling cells and are strongly induced by replication stress. In addition to replication stress response proteins, GMGs contain factors involved in RNA metabolism as well as CDK1. Depletion of TIAR accelerates mitotic entry and leads to chromosomal instability in response to replication stress, in a manner that can be alleviated by the concomitant depletion of Cdc25B or inhibition of CDK1. Since TIAR retains CDK1 in GMGs and attenuates CDK1 activity, we propose that the assembly of GMGs may represent a so far unrecognized mechanism that contributes to the activation of the G2/M checkpoint in mammalian cells.", "doi": "10.15252/embr.201846224", "pmid": "30538118", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC6322364"}, {"db": "pii", "key": "embr.201846224"}], "notes": [], "created": "2026-08-20T12:51:44.825Z", "modified": "2026-08-20T12:51:44.967Z"}]}