{"entity": "researcher", "timestamp": "2026-09-23T19:10:28.681Z", "family": "Bondar", "given": "Alexander", "initials": "A", "orcid": "0000-0001-9181-0487", "affiliations": ["Institute of Chemical Biology and Fundamental Medicine, 630090 Novosibirsk, Russia. alex.bondar@mail.ru."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/61ced1ae8b9c42bdb27ae7749838929a.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/61ced1ae8b9c42bdb27ae7749838929a"}}, "publications": [{"entity": "publication", "iuid": "9a3c2c594213406b9cd80d38e42e7456", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/9a3c2c594213406b9cd80d38e42e7456.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/9a3c2c594213406b9cd80d38e42e7456"}}, "title": "Regulation of Neuronal Na,K-ATPase by Extracellular Scaffolding Proteins.", "authors": [{"family": "Liebmann", "given": "Thomas", "initials": "T"}, {"family": "Fritz", "given": "Nicolas", "initials": "N", "orcid": "0000-0002-9722-7425", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c191a09238064a52b559e43ee867de61.json"}}, {"family": "Kruusm\u00e4gi", "given": "Markus", "initials": "M"}, {"family": "Westin", "given": "Linda", "initials": "L"}, {"family": "Bernhem", "given": "Kristoffer", "initials": "K"}, {"family": "Bondar", "given": "Alexander", "initials": "A", "orcid": "0000-0001-9181-0487", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/61ced1ae8b9c42bdb27ae7749838929a.json"}}, {"family": "Aperia", "given": "Anita", "initials": "A"}, {"family": "Brismar", "given": "Hjalmar", "initials": "H", "orcid": "0000-0003-0578-4003", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/04321d9fb805493db538489927a42c8f.json"}}], "type": "journal article", "published": "2018-07-29", "journal": {"title": "Int J Mol Sci", "issn": "1422-0067", "volume": "19", "issue": "8", "issn-l": null}, "abstract": "Neuronal activity leads to an influx of Na\u207a that needs to be rapidly cleared. The sodium-potassium ATPase (Na,K-ATPase) exports three Na\u207a ions and imports two K\u207a ions at the expense of one ATP molecule. Na,K-ATPase turnover accounts for the majority of energy used by the brain. To prevent an energy crisis, the energy expense for Na\u207a clearance must provide an optimal effect. Here we report that in rat primary hippocampal neurons, the clearance of Na\u207a ions is more efficient if Na,K-ATPase is laterally mobile in the membrane than if it is clustered. Using fluorescence recovery after photobleaching and single particle tracking analysis, we show that the ubiquitous \u03b11 and the neuron-specific \u03b13 catalytic subunits as well as the supportive \u03b21 subunit of Na,K-ATPase are highly mobile in the plasma membrane. We show that cross-linking of the \u03b21 subunit with polyclonal antibodies or exposure to Modulator of Na,K-ATPase (MONaKA), a secreted protein which binds to the extracellular domain of the \u03b2 subunit, clusters the \u03b13 subunit in the membrane and restricts its mobility. We demonstrate that clustering, caused by cross-linking or by exposure to MONaKA, reduces the efficiency in restoring intracellular Na\u207a. These results demonstrate that extracellular interactions with Na,K-ATPase regulate the Na\u207a extrusion efficiency with consequences for neuronal energy balance.", "doi": "10.3390/ijms19082214", "pmid": "30060621", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC6121408"}, {"db": "pii", "key": "ijms19082214"}], "notes": [], "created": "2026-09-23T12:39:18.258Z", "modified": "2026-09-23T12:39:18.403Z"}]}