{"entity": "researcher", "timestamp": "2026-08-20T21:03:22.365Z", "family": "Welinder", "given": "Charlotte", "initials": "C", "orcid": "0000-0001-9626-0576", "affiliations": ["Department of Clinical Sciences Lund, Section of Oncology, Lund University, Lund 221 85, Sweden."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/5715a70ce8df4a5d84ad88709791772a.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/5715a70ce8df4a5d84ad88709791772a"}}, "publications": [{"entity": "publication", "iuid": "3e361ab03510479f99e72aeadd8afd37", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/3e361ab03510479f99e72aeadd8afd37.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/3e361ab03510479f99e72aeadd8afd37"}}, "title": "Landscape of surfaceome and endocytome in human glioma is divergent and depends on cellular spatial organization.", "authors": [{"family": "Governa", "given": "Valeria", "initials": "V"}, {"family": "Talbot", "given": "Hugo", "initials": "H", "orcid": "0000-0001-9774-8573", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/28e1c9cf68e6408dbcf4ac2c031fb468.json"}}, {"family": "Gon\u00e7alves de Oliveira", "given": "Kelin", "initials": "K", "orcid": "0000-0003-1646-5657", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f774f99247314765b97114edc82199f1.json"}}, {"family": "Cerezo-Maga\u00f1a", "given": "Myriam", "initials": "M", "orcid": "0000-0001-9752-8917", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3c8019362baf45b394e839c00f7ac1d5.json"}}, {"family": "B\u00e5ng-Rudenstam", "given": "Anna", "initials": "A", "orcid": "0000-0003-2706-9502", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3fea3b51f73f4175b859cde49e99e5e7.json"}}, {"family": "Johansson", "given": "Maria C", "initials": "MC", "orcid": "0000-0003-0075-3187", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1ce5f12cf2e24399b8b1a2f90a647e6e.json"}}, {"family": "M\u00e5nsson", "given": "Ann-Sofie", "initials": "AS", "orcid": "0000-0002-5984-3470", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9b3c8e6d27ad4029be4738d5816e0234.json"}}, {"family": "Forsberg-Nilsson", "given": "Karin", "initials": "K"}, {"family": "Marko-Varga", "given": "Gy\u00f6rgy", "initials": "G"}, {"family": "Enr\u00edquez P\u00e9rez", "given": "Julio", "initials": "J", "orcid": "0000-0002-6361-3272", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/836f9e769c34462ea7d6d9ac64345beb.json"}}, {"family": "Darabi", "given": "Anna", "initials": "A", "orcid": "0000-0003-4326-8149", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/bb9f19a0c8694ee79ca515a79976d146.json"}}, {"family": "Malmstr\u00f6m", "given": "Johan", "initials": "J", "orcid": "0000-0002-2889-7169", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c89da9268a4f47a8a99fd62de451aaa7.json"}}, {"family": "Bengzon", "given": "Johan", "initials": "J"}, {"family": "Welinder", "given": "Charlotte", "initials": "C", "orcid": "0000-0001-9626-0576", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5715a70ce8df4a5d84ad88709791772a.json"}}, {"family": "Belting", "given": "Mattias", "initials": "M", "orcid": "0000-0003-1585-5434", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d9763a47486d410fb5034fb2603f3a02.json"}}], "type": "journal article", "published": "2022-03-01", "journal": {"title": "Proc. Natl. Acad. Sci. U.S.A.", "issn": "1091-6490", "volume": "119", "issue": "9", "issn-l": "0027-8424"}, "abstract": "Therapeutic strategies directed at the tumor surfaceome (TS), including checkpoint inhibitor blocking antibodies, antibody drug conjugates (ADCs), and chimeric antigen receptor T (CAR-T) cells, provide a new armament to fight cancer. However, a remaining bottleneck is the lack of strategies to comprehensively interrogate patient tumors for potential TS targets. Here, we have developed a platform (tumor surfaceome mapping [TS-MAP]) integrated with a newly curated TS classifier (SURFME) that allows profiling of primary 3D cultures and intact patient glioma tumors with preserved tissue architecture. Moreover, TS-MAP specifically identifies proteins capable of endocytosis as tractable targets for ADCs and other modalities requiring toxic payload internalization. In high-grade gliomas that remain among the most aggressive forms of cancer, we show that cellular spatial organization (2D vs. 3D) fundamentally transforms the surfaceome and endocytome (e.g., integrins, proteoglycans, semaphorins, and cancer stem cell markers) with general implications for target screening approaches, as exemplified by an ADC targeting EGFR. The TS-MAP platform was further applied to profile the surfaceome and endocytome landscape in a cohort of freshly resected gliomas. We found a highly diverse TS repertoire between patient tumors, not directly associated with grade and histology, which highlights the need for individualized approaches. Our data provide additional layers of understanding fundamental to the future development of immunotherapy strategies, as well as procedures for proteomics-based target identification and selection. The TS-MAP platform should be widely applicable in efforts aiming at a better understanding of how to harness the TS for personalized immunotherapy.", "doi": "10.1073/pnas.2114456119", "pmid": "35217608", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC8892282"}, {"db": "pii", "key": "2114456119"}], "notes": [], "created": "2026-08-20T09:31:01.542Z", "modified": "2026-08-20T09:31:01.946Z"}, {"entity": "publication", "iuid": "e4c03509339a4a7487c366bc3827bc47", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/e4c03509339a4a7487c366bc3827bc47.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/e4c03509339a4a7487c366bc3827bc47"}}, "title": "Ultrasensitive Immunoprofiling of Plasma Extracellular Vesicles Identifies Syndecan-1 as a Potential Tool for Minimally Invasive Diagnosis of Glioma.", "authors": [{"family": "Indira Chandran", "given": "Vineesh", "initials": "V"}, {"family": "Welinder", "given": "Charlotte", "initials": "C", "orcid": "0000-0001-9626-0576", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5715a70ce8df4a5d84ad88709791772a.json"}}, {"family": "M\u00e5nsson", "given": "Ann-Sofie", "initials": "AS"}, {"family": "Offer", "given": "Svenja", "initials": "S"}, {"family": "Freyhult", "given": "Eva", "initials": "E", "orcid": "0000-0003-0226-1047", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/13486bb11790445fa37f592a42e6a7ed.json"}}, {"family": "Pernemalm", "given": "Maria", "initials": "M", "orcid": "0000-0003-4624-031X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c34e0806a3d142019ae8e16de8d720ef.json"}}, {"family": "Lund", "given": "Sigrid M", "initials": "SM"}, {"family": "Pedersen", "given": "Shona", "initials": "S", "orcid": "0000-0001-6636-0293", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/daa11d544a2545b0aa2a7ec6083fc079.json"}}, {"family": "Lehti\u00f6", "given": "Janne", "initials": "J"}, {"family": "Marko-Varga", "given": "Gyorgy", "initials": "G"}, {"family": "Johansson", "given": "Maria C", "initials": "MC"}, {"family": "Englund", "given": "Elisabet", "initials": "E", "orcid": "0000-0002-2708-2443", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8d415783747141269a6d9a843afc861d.json"}}, {"family": "Sundgren", "given": "Pia C", "initials": "PC", "orcid": "0000-0001-9237-1236", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3e580179d7d74958a5c1d509e581ddcc.json"}}, {"family": "Belting", "given": "Mattias", "initials": "M"}], "type": "journal article", "published": "2019-05-15", "journal": {"title": "Clin. Cancer Res.", "issn": "1557-3265", "volume": "25", "issue": "10", "pages": "3115-3127", "issn-l": "1078-0432"}, "abstract": "Liquid biopsy has great potential to improve the management of brain tumor patients at high risk of surgery-associated complications. Here, the aim was to explore plasma extracellular vesicle (plEV) immunoprofiling as a tool for noninvasive diagnosis of glioma.\n\nPlEV isolation and analysis were optimized using advanced mass spectrometry, nanoparticle tracking analysis, and electron microscopy. We then established a new procedure that combines size exclusion chromatography isolation and proximity extension assay-based ultrasensitive immunoprofiling of plEV proteins that was applied on a well-defined glioma study cohort (n = 82).\n\nAmong potential candidates, we for the first time identify syndecan-1 (SDC1) as a plEV constituent that can discriminate between high-grade glioblastoma multiforme (GBM, WHO grade IV) and low-grade glioma [LGG, WHO grade II; area under the ROC curve (AUC): 0.81; sensitivity: 71%; specificity: 91%]. These findings were independently validated by ELISA. Tumor SDC1 mRNA expression similarly discriminated between GBM and LGG in an independent glioma patient population from The Cancer Genome Atlas cohort (AUC: 0.91; sensitivity: 79%; specificity: 91%). In experimental studies with GBM cells, we show that SDC1 is efficiently sorted to secreted EVs. Importantly, we found strong support of plEVSDC1 originating from GBM tumors, as plEVSDC1 correlated with SDC1 protein expression in matched patient tumors, and plEVSDC1 was decreased postoperatively depending on the extent of surgery.\n\nOur studies support the concept of circulating plEVs as a tool for noninvasive diagnosis and monitoring of gliomas and should move this field closer to the goal of improving the management of cancer patients.", "doi": "10.1158/1078-0432.CCR-18-2946", "pmid": "30679164", "labels": [], "xrefs": [{"db": "pii", "key": "1078-0432.CCR-18-2946"}], "notes": [], "created": "2026-08-20T12:12:08.365Z", "modified": "2026-08-20T12:12:08.608Z"}]}