{"entity": "researcher", "timestamp": "2026-09-08T10:05:36.381Z", "family": "Lyngso", "given": "Kristina S", "initials": "KS", "orcid": "0000-0002-3250-4049", "affiliations": ["Department of Cardiovascular and Renal Research, Institute of Molecular Medicine, University of Southern Denmark, Odense, Denmark."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/51d04f9fc14d47e59eeb90a38efa553b.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/51d04f9fc14d47e59eeb90a38efa553b"}}, "publications": [{"entity": "publication", "iuid": "9a46a448c23e4644b3ac6ef85b645583", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/9a46a448c23e4644b3ac6ef85b645583.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/9a46a448c23e4644b3ac6ef85b645583"}}, "title": "Inhibition of cystathionine-gamma lyase dampens vasoconstriction in mouse and human intracerebral arterioles.", "authors": [{"family": "Peleli", "given": "Maria", "initials": "M"}, {"family": "Lyngso", "given": "Kristina S", "initials": "KS", "orcid": "0000-0002-3250-4049", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/51d04f9fc14d47e59eeb90a38efa553b.json"}}, {"family": "Poulsen", "given": "Frantz Rom", "initials": "FR"}, {"family": "Hansen", "given": "Pernille B L", "initials": "PBL"}, {"family": "Papapetropoulos", "given": "Andreas", "initials": "A"}, {"family": "Stubbe", "given": "Jane", "initials": "J", "orcid": "0000-0002-2703-453X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d4302be961454e2087fb4f8db427eb37.json"}}], "type": "journal article", "published": "2023-09-00", "journal": {"title": "Acta Physiol", "issn": "1748-1716", "volume": "239", "issue": "1", "pages": "e14021", "issn-l": "1748-1708"}, "abstract": "In extracerebral vascular beds cystathionine-gamma lyase (CSE) activity plays a vasodilatory role but the role of this hydrogen sulfide (H2 S) producing enzyme in the intracerebral arterioles remain poorly understood. We hypothesized a similar function in the intracerebral arterioles.\n\nIntracerebral arterioles were isolated from wild type C57BL/6J mouse (9-12 months old) brains and from human brain biopsies. The function (contractility and secondary dilatation) of the intracerebral arterioles was tested ex vivo by pressure myography using a perfusion set-up. Reverse transcription polymerase chain reaction was used for detecting CSE expression.\n\nCSE is expressed in human and mouse intracerebral arterioles. CSE inhibition with L-propargylglycine (PAG) significantly dampened the K+ -induced vasoconstriction in intracerebral arterioles of both species (% of maximum contraction: in human control: 45.4 \u00b1 2.7 versus PAG: 27 \u00b1 5.2 and in mouse control: 50 \u00b1 1.5 versus PAG: 33 \u00b1 5.2) but did not affect the secondary dilatation. This effect of PAG was significantly reversed by the H2 S donor sodium hydrosulfide (NaSH) in human (PAG + NaSH: 38.8 \u00b1 7.2) and mouse (PAG + NaSH: 41.7 \u00b1 3.1) arterioles, respectively. The endothelial NO synthase (eNOS) inhibitor, N\u03c9-Nitro-l-arginine methyl ester (L-NAME), and the inhibitor of soluble guanylate cyclase (sGC), 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ) reversed the effect of PAG on the K+ -induced vasoconstriction in the mouse arterioles and attenuated the K+ -induced secondary dilatation significantly.\n\nCSE contributes to the K+ -induced vasoconstriction via a mechanism involving H2 S, eNOS, and sGC whereas the secondary dilatation is regulated by eNOS and sGC but not by CSE.", "doi": "10.1111/apha.14021", "pmid": "37555636", "labels": [], "xrefs": [], "notes": [], "created": "2026-08-21T12:17:18.229Z", "modified": "2026-08-21T12:17:18.368Z"}]}