{"entity": "researcher", "timestamp": "2026-08-22T06:58:12.942Z", "family": "Garc\u00eda-G\u00f3mez", "given": "Pedro", "initials": "P", "orcid": "0000-0003-0694-3942", "affiliations": ["Department of Medical Biochemistry and Microbiology, Science for Life Laboratory, Biomedical Center, Uppsala University, Sweden.", "Ludwig Cancer Research, Science for Life Laboratory, Biomedical Center, Uppsala University, Sweden."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/5122d492559c4e90a87d75af3b524bd4.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/5122d492559c4e90a87d75af3b524bd4"}}, "publications": [{"entity": "publication", "iuid": "f1acd3c41ff64c4a9aeb991052cfdb90", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/f1acd3c41ff64c4a9aeb991052cfdb90.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/f1acd3c41ff64c4a9aeb991052cfdb90"}}, "title": "NOX4 regulates TGF\u03b2-induced proliferation and self-renewal in glioblastoma stem cells.", "authors": [{"family": "Garc\u00eda-G\u00f3mez", "given": "Pedro", "initials": "P", "orcid": "0000-0003-0694-3942", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5122d492559c4e90a87d75af3b524bd4.json"}}, {"family": "Gol\u00e1n", "given": "Irene", "initials": "I"}, {"family": "S Dadras", "given": "Mahsa", "initials": "M"}, {"family": "Mezheyeuski", "given": "Artur", "initials": "A"}, {"family": "Bellomo", "given": "Claudia", "initials": "C"}, {"family": "Tzavlaki", "given": "Kalliopi", "initials": "K"}, {"family": "Mor\u00e9n", "given": "Anita", "initials": "A"}, {"family": "Carreras-Puigvert", "given": "Jordi", "initials": "J"}, {"family": "Caja", "given": "Laia", "initials": "L", "orcid": "0000-0002-8786-8763", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5f8e6b4e82274addbcaede8de73ad5d1.json"}}], "type": "journal article", "published": "2022-05-00", "journal": {"title": "Mol Oncol", "issn": "1878-0261", "volume": "16", "issue": "9", "pages": "1891-1912", "issn-l": "1574-7891"}, "abstract": "Glioblastoma (GBM) is the most aggressive and common glioma subtype, with a median survival of 15 months after diagnosis. Current treatments have limited therapeutic efficacy; thus, more effective approaches are needed. The glioblastoma tumoural mass is characterised by a small cellular subpopulation - glioblastoma stem cells (GSCs) - that has been held responsible for glioblastoma initiation, cell invasion, proliferation, relapse and resistance to chemo- and radiotherapy. Targeted therapies against GSCs are crucial, as is understanding the molecular mechanisms that govern the GSCs. Transforming growth factor \u03b2 (TGF\u03b2) signalling and reactive oxygen species (ROS) production are known to govern and regulate cancer stem cell biology. Among the differentially expressed genes regulated by TGF\u03b2 in a transcriptomic analysis of two different patient-derived GSCs, we found NADPH oxidase 4 (NOX4) as one of the top upregulated genes. Interestingly, when patient tissues were analysed, NOX4 expression was found to be higher in GSCs versus differentiated cells. A functional analysis of the role of NOX4 downstream of TGF\u03b2 in several patient-derived GSCs showed that TGF\u03b2 does indeed induce NOX4 expression and increases ROS production in a NOX4-dependent manner. NOX4 downstream of TGF\u03b2 regulates GSC proliferation, and NOX4 expression is necessary for TGF\u03b2-induced expression of stem cell markers and of the transcription factor nuclear factor erythroid 2-related factor 2 (NRF2), which in turn controls the cell's antioxidant and metabolic responses. Interestingly, overexpression of NOX4 recapitulates the effects induced by TGF\u03b2 in GSCs: enhanced proliferation, stemness and NRF2 expression. In conclusion, this work functionally establishes NOX4 as a key mediator of GSC biology.", "doi": "10.1002/1878-0261.13200", "pmid": "35203105", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC9067149"}], "notes": [], "created": "2026-08-21T10:58:12.828Z", "modified": "2026-08-21T10:58:12.974Z"}]}