{"entity": "researcher", "timestamp": "2026-09-24T14:45:54.195Z", "family": "Strittmatter", "given": "Nicole", "initials": "N", "orcid": "0000-0003-1277-9608", "affiliations": ["Imaging & Data Analytics, Clinical Pharmacology and Safety Sciences, BioPharmaceuticals R&D, AstraZeneca, Cambridge, UK."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/5105cdf7970d487eb012007a325fc947.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/5105cdf7970d487eb012007a325fc947"}}, "publications": [{"entity": "publication", "iuid": "02f1a50ed77e437ab913b5a6a50dd409", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/02f1a50ed77e437ab913b5a6a50dd409.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/02f1a50ed77e437ab913b5a6a50dd409"}}, "title": "Method To Visualize the Intratumor Distribution and Impact of Gemcitabine in Pancreatic Ductal Adenocarcinoma by Multimodal Imaging.", "authors": [{"family": "Strittmatter", "given": "Nicole", "initials": "N", "orcid": "0000-0003-1277-9608", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5105cdf7970d487eb012007a325fc947.json"}}, {"family": "Richards", "given": "Frances M", "initials": "FM"}, {"family": "Race", "given": "Alan M", "initials": "AM", "orcid": "0000-0001-8996-2641", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/7f8e03d7684e4434ab6fb96c5727f828.json"}}, {"family": "Ling", "given": "Stephanie", "initials": "S", "orcid": "0000-0002-1237-091X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5ad32efeb59c4a60b4f2017d711a2863.json"}}, {"family": "Sutton", "given": "Daniel", "initials": "D"}, {"family": "Nilsson", "given": "Anna", "initials": "A"}, {"family": "Wallez", "given": "Yann", "initials": "Y"}, {"family": "Barnes", "given": "Jennifer", "initials": "J"}, {"family": "Maglennon", "given": "Gareth", "initials": "G"}, {"family": "Gopinathan", "given": "Aarthi", "initials": "A"}, {"family": "Brais", "given": "Rebecca", "initials": "R"}, {"family": "Wong", "given": "Edmond", "initials": "E"}, {"family": "Serra", "given": "Maria Paola", "initials": "MP"}, {"family": "Atkinson", "given": "James", "initials": "J"}, {"family": "Smith", "given": "Aaron", "initials": "A"}, {"family": "Wilson", "given": "Joanne", "initials": "J"}, {"family": "Hamm", "given": "Gregory", "initials": "G"}, {"family": "Johnson", "given": "Timothy I", "initials": "TI"}, {"family": "Dunlop", "given": "Charles R", "initials": "CR"}, {"family": "Kaistha", "given": "Brajesh P", "initials": "BP"}, {"family": "Bunch", "given": "Josephine", "initials": "J"}, {"family": "Sansom", "given": "Owen J", "initials": "OJ"}, {"family": "Takats", "given": "Zoltan", "initials": "Z", "orcid": "0000-0002-0795-3467", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f7f7a02c9df641a9bc21ffbeca989d47.json"}}, {"family": "Andr\u00e9n", "given": "Per E", "initials": "PE", "orcid": "0000-0002-4062-7743", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f1dd27e8b238475383983480e10099f6.json"}}, {"family": "Lau", "given": "Alan", "initials": "A"}, {"family": "Barry", "given": "Simon T", "initials": "ST"}, {"family": "Goodwin", "given": "Richard J A", "initials": "RJA"}, {"family": "Jodrell", "given": "Duncan I", "initials": "DI"}], "type": "journal article", "published": "2022-01-25", "journal": {"title": "Anal. Chem.", "issn": "1520-6882", "volume": "94", "issue": "3", "pages": "1795-1803", "issn-l": "0003-2700"}, "abstract": "Gemcitabine (dFdC) is a common treatment for pancreatic cancer; however, it is thought that treatment may fail because tumor stroma prevents drug distribution to tumor cells. Gemcitabine is a pro-drug with active metabolites generated intracellularly; therefore, visualizing the distribution of parent drug as well as its metabolites is important. A multimodal imaging approach was developed using spatially coregistered mass spectrometry imaging (MSI), imaging mass cytometry (IMC), multiplex immunofluorescence microscopy (mIF), and hematoxylin and eosin (H&E) staining to assess the local distribution and metabolism of gemcitabine in tumors from a genetically engineered mouse model of pancreatic cancer (KPC) allowing for comparisons between effects in the tumor tissue and its microenvironment. Mass spectrometry imaging (MSI) enabled the visualization of the distribution of gemcitabine (100 mg/kg), its phosphorylated metabolites dFdCMP, dFdCDP and dFdCTP, and the inactive metabolite dFdU. Distribution was compared to small-molecule ATR inhibitor AZD6738 (25 mg/kg), which was codosed. Gemcitabine metabolites showed heterogeneous distribution within the tumor, which was different from the parent compound. The highest abundance of dFdCMP, dFdCDP, and dFdCTP correlated with distribution of endogenous AMP, ADP, and ATP in viable tumor cell regions, showing that gemcitabine active metabolites are reaching the tumor cell compartment, while AZD6738 was located to nonviable tumor regions. The method revealed that the generation of active, phosphorylated dFdC metabolites as well as treatment-induced DNA damage primarily correlated with sites of high proliferation in KPC PDAC tumor tissue, rather than sites of high parent drug abundance.", "doi": "10.1021/acs.analchem.1c04579", "pmid": "35005896", "labels": [], "xrefs": [], "notes": [], "created": "2026-09-23T09:35:44.799Z", "modified": "2026-09-23T09:35:44.916Z"}, {"entity": "publication", "iuid": "0ea7c02e5164463b93ef5bb4dbfb6763", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/0ea7c02e5164463b93ef5bb4dbfb6763.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/0ea7c02e5164463b93ef5bb4dbfb6763"}}, "title": "Holistic Characterization of a Salmonella Typhimurium Infection Model Using Integrated Molecular Imaging.", "authors": [{"family": "Strittmatter", "given": "Nicole", "initials": "N", "orcid": "0000-0003-1277-9608", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5105cdf7970d487eb012007a325fc947.json"}}, {"family": "Kanvatirth", "given": "Panchali", "initials": "P"}, {"family": "Inglese", "given": "Paolo", "initials": "P", "orcid": "0000-0001-6179-9643", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/399f7b70327641c3983ff0b320e4d299.json"}}, {"family": "Race", "given": "Alan M", "initials": "AM", "orcid": "0000-0001-8996-2641", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/7f8e03d7684e4434ab6fb96c5727f828.json"}}, {"family": "Nilsson", "given": "Anna", "initials": "A"}, {"family": "Dannhorn", "given": "Andreas", "initials": "A", "orcid": "0000-0002-1087-4057", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/842705f631e44ff1bbdcacfc24505168.json"}}, {"family": "Kudo", "given": "Hiromi", "initials": "H"}, {"family": "Goldin", "given": "Robert D", "initials": "RD"}, {"family": "Ling", "given": "Stephanie", "initials": "S", "orcid": "0000-0002-1237-091X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5ad32efeb59c4a60b4f2017d711a2863.json"}}, {"family": "Wong", "given": "Edmond", "initials": "E"}, {"family": "Seeliger", "given": "Frank", "initials": "F"}, {"family": "Serra", "given": "Maria Paola", "initials": "MP"}, {"family": "Hoffmann", "given": "Scott", "initials": "S"}, {"family": "Maglennon", "given": "Gareth", "initials": "G"}, {"family": "Hamm", "given": "Gregory", "initials": "G"}, {"family": "Atkinson", "given": "James", "initials": "J"}, {"family": "Jones", "given": "Stewart", "initials": "S"}, {"family": "Bunch", "given": "Josephine", "initials": "J"}, {"family": "Andr\u00e9n", "given": "Per E", "initials": "PE", "orcid": "0000-0002-4062-7743", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f1dd27e8b238475383983480e10099f6.json"}}, {"family": "Takats", "given": "Zoltan", "initials": "Z", "orcid": "0000-0002-0795-3467", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f7f7a02c9df641a9bc21ffbeca989d47.json"}}, {"family": "Goodwin", "given": "Richard J A", "initials": "RJA"}, {"family": "Mastroeni", "given": "Pietro", "initials": "P"}], "type": "journal article", "published": "2021-12-01", "journal": {"title": "J. Am. Soc. Mass Spectrom.", "issn": "1879-1123", "volume": "32", "issue": "12", "pages": "2791-2802", "issn-l": "1044-0305"}, "abstract": "A more complete and holistic view on host-microbe interactions is needed to understand the physiological and cellular barriers that affect the efficacy of drug treatments and allow the discovery and development of new therapeutics. Here, we developed a multimodal imaging approach combining histopathology with mass spectrometry imaging (MSI) and same section imaging mass cytometry (IMC) to study the effects of Salmonella Typhimurium infection in the liver of a mouse model using the S. Typhimurium strains SL3261 and SL1344. This approach enables correlation of tissue morphology and specific cell phenotypes with molecular images of tissue metabolism. IMC revealed a marked increase in immune cell markers and localization in immune aggregates in infected tissues. A correlative computational method (network analysis) was deployed to find metabolic features associated with infection and revealed metabolic clusters of acetyl carnitines, as well as phosphatidylcholine and phosphatidylethanolamine plasmalogen species, which could be associated with pro-inflammatory immune cell types. By developing an IMC marker for the detection of Salmonella LPS, we were further able to identify and characterize those cell types which contained S. Typhimurium.", "doi": "10.1021/jasms.1c00240", "pmid": "34767352", "labels": [], "xrefs": [], "notes": [], "created": "2026-09-23T12:52:23.725Z", "modified": "2026-09-23T12:52:23.909Z"}, {"entity": "publication", "iuid": "15171e08311d46e3be6b481a44c1eddd", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/15171e08311d46e3be6b481a44c1eddd.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/15171e08311d46e3be6b481a44c1eddd"}}, "title": "Spatial visualization of comprehensive brain neurotransmitter systems and neuroactive substances by selective in situ chemical derivatization mass spectrometry imaging.", "authors": [{"family": "Shariatgorji", "given": "Reza", "initials": "R", "orcid": "0000-0001-9484-0921", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/76faa76e9dc445a3bbb1f470e9d33a23.json"}}, {"family": "Nilsson", "given": "Anna", "initials": "A"}, {"family": "Fridjonsdottir", "given": "Elva", "initials": "E"}, {"family": "Strittmatter", "given": "Nicole", "initials": "N", "orcid": "0000-0003-1277-9608", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5105cdf7970d487eb012007a325fc947.json"}}, {"family": "Dannhorn", "given": "Andreas", "initials": "A", "orcid": "0000-0002-1087-4057", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/842705f631e44ff1bbdcacfc24505168.json"}}, {"family": "Svenningsson", "given": "Per", "initials": "P"}, {"family": "Goodwin", "given": "Richard J A", "initials": "RJA", "orcid": "0000-0001-5699-8488", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/debf1fe0756d4ad986bf74605fce924c.json"}}, {"family": "Odell", "given": "Luke R", "initials": "LR"}, {"family": "Andr\u00e9n", "given": "Per E", "initials": "PE", "orcid": "0000-0002-4062-7743", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f1dd27e8b238475383983480e10099f6.json"}}], "type": "journal article", "published": "2021-07-00", "journal": {"title": "Nat Protoc", "issn": "1750-2799", "volume": "16", "issue": "7", "pages": "3298-3321", "issn-l": null}, "abstract": "Molecule-specific techniques such as MALDI and desorption electrospray ionization mass spectrometry imaging enable direct and simultaneous mapping of biomolecules in tissue sections in a single experiment. However, neurotransmitter imaging in the complex environment of biological samples remains challenging. Our covalent charge-tagging approach using on-tissue chemical derivatization of primary and secondary amines and phenolic hydroxyls enables comprehensive mapping of neurotransmitter networks. Here, we present robust and easy-to-use chemical derivatization protocols that facilitate quantitative and simultaneous molecular imaging of complete neurotransmitter systems and drugs in diverse biological tissue sections with high lateral resolution. This is currently not possible with any other imaging technique. The protocol, using fluoromethylpyridinium and pyrylium reagents, describes all steps from tissue preparation (~1 h), chemical derivatization (1-2 h), data collection (timing depends on the number of samples and lateral resolution) and data analysis and interpretation. The specificity of the chemical reaction can also help users identify unknown chemical identities. Our protocol can reveal the cellular locations in which signaling molecules act and thus shed light on the complex responses that occur after the administration of drugs or during the course of a disease.", "doi": "10.1038/s41596-021-00538-w", "pmid": "34075230", "labels": [], "xrefs": [{"db": "pii", "key": "10.1038/s41596-021-00538-w"}], "notes": [], "created": "2026-09-23T08:37:14.077Z", "modified": "2026-09-23T08:37:14.252Z"}]}