{"entity": "researcher", "timestamp": "2026-08-22T06:56:50.562Z", "family": "Vessby", "given": "Johan", "initials": "J", "orcid": "0000-0003-1832-6386", "affiliations": ["Department of Medical Sciences, Gastroenterology Research Group, Uppsala University, Uppsala, Sweden."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/4ed9e8d51dec4076855ed37e013bf22e.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/4ed9e8d51dec4076855ed37e013bf22e"}}, "publications": [{"entity": "publication", "iuid": "1cb9a60565604e1f9d6bb34b67615dbc", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/1cb9a60565604e1f9d6bb34b67615dbc.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/1cb9a60565604e1f9d6bb34b67615dbc"}}, "title": "AGPAT1 as a Novel Colonic Biomarker for Discriminating Between Ulcerative Colitis With and Without Primary Sclerosing Cholangitis.", "authors": [{"family": "Vessby", "given": "Johan", "initials": "J", "orcid": "0000-0003-1832-6386", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4ed9e8d51dec4076855ed37e013bf22e.json"}}, {"family": "Wisniewski", "given": "Jacek R", "initials": "JR"}, {"family": "Lindskog", "given": "Cecilia", "initials": "C"}, {"family": "Eriksson", "given": "Niclas", "initials": "N"}, {"family": "Gabrysch", "given": "Katja", "initials": "K"}, {"family": "Zettl", "given": "Katharina", "initials": "K"}, {"family": "Wanders", "given": "Alkwin", "initials": "A"}, {"family": "Carlson", "given": "Marie", "initials": "M"}, {"family": "Rorsman", "given": "Fredrik", "initials": "F"}, {"family": "\u00c5berg", "given": "Mikael", "initials": "M"}], "type": "journal article", "published": "2022-05-01", "journal": {"title": "Clin Transl Gastroenterol", "issn": "2155-384X", "volume": "13", "issue": "5", "pages": "e00486", "issn-l": null}, "abstract": "Ulcerative colitis (UC) associated with primary sclerosing cholangitis (PSC-UC) is considered a unique inflammatory bowel disease (IBD) entity. PSC diagnosis in an IBD individual entails a significantly higher risk of gastrointestinal cancer; however, biomarkers for identifying patients with UC at risk for PSC are lacking. We, therefore, performed a thorough PSC-UC biomarker study, starting from archived colonic tissue.\n\nProteins were extracted out of formalin-fixed paraffin-embedded proximal colon samples from PSC-UC (n = 9), UC (n = 7), and healthy controls (n = 7). Patients with IBD were in clinical and histological remission, and all patients with UC had a history of pancolitis. Samples were processed by the multienzyme digestion FASP and subsequently analyzed by liquid chromatography-tandem mass spectrometry. Candidate proteins were replicated in an independent cohort (n: PSC-UC = 16 and UC = 21) and further validated by immunohistochemistry.\n\nIn the discovery step, 7,279 unique proteins were detected. The top 5 most differentiating proteins (PSC-UC vs UC) based on linear regression analysis were selected for replication. Of these, 1-acetylglycerol-3-phosphate O-acyltransferase 1 (AGPAT1) was verified as higher in PSC-UC than UC (P = 0.009) in the replication cohort. A difference on the group level was also confirmed by immunohistochemistry, showing more intense AGPAT1 staining in patients with PSC-UC compared with UC.\n\nWe present AGPAT1 as a potential colonic biomarker for differentiating PSC-UC from UC. Our findings have possible implication for future PSC-IBD diagnostics and surveillance.", "doi": "10.14309/ctg.0000000000000486", "pmid": "35363634", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC9132532"}, {"db": "pii", "key": "01720094-202205000-00013"}], "notes": [], "created": "2026-08-21T12:53:06.775Z", "modified": "2026-08-21T12:53:06.815Z"}, {"entity": "publication", "iuid": "360a66fffea242aba6fda6bdd416362b", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/360a66fffea242aba6fda6bdd416362b.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/360a66fffea242aba6fda6bdd416362b"}}, "title": "Tissue factor in ulcerative colitis, with and without concomitant primary sclerosing cholangitis.", "authors": [{"family": "Vessby", "given": "Johan", "initials": "J", "orcid": "0000-0003-1832-6386", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4ed9e8d51dec4076855ed37e013bf22e.json"}}, {"family": "Lampinen", "given": "Maria", "initials": "M", "orcid": "0000-0002-0396-2147", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6b9fc30c0e264d98950d0d2c84b29e8d.json"}}, {"family": "\u00c5berg", "given": "Mikael", "initials": "M", "orcid": "0000-0002-7858-8233", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a08650cb00404b6dba35959f3cb0a53c.json"}}, {"family": "Rorsman", "given": "Fredrik", "initials": "F", "orcid": "0000-0003-4023-9617", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/bad7c76206484992baeed8ae59dc8a30.json"}}, {"family": "Siegbahn", "given": "Agneta", "initials": "A", "orcid": "0000-0003-3955-5671", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/2d92de12af1248d5ae536c4d717ebb77.json"}}, {"family": "Wanders", "given": "Alkwin", "initials": "A", "orcid": "0000-0002-1690-6363", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4b4a33238339408ea3a0e9e19df186fc.json"}}, {"family": "Carlson", "given": "Marie", "initials": "M", "orcid": "0000-0002-2402-9305", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0c4178131f6646828421947e70ec396c.json"}}], "type": "journal article", "published": "2019-11-00", "journal": {"title": "Ups. J. Med. Sci.", "issn": "2000-1967", "volume": "124", "issue": "4", "pages": "238-245", "issn-l": "0300-9734"}, "abstract": "Background: Ulcerative colitis (UC) in patients with the severe disease primary sclerosing cholangitis (PSC) constitutes a distinct clinical phenotype (PSC-UC) with a high incidence of colorectal cancer. Today, PSC-UC diagnosis is built on clinical observations only. Tissue factor (TF) has a potential use in UC diagnostics, and also in colorectal cancer prognostication. Here we evaluate TF expression in an inflammatory bowel disease (IBD) cohort, with special focus on differences between UC and PSC-UC patients.Materials and methods: Colonic biopsies from UC (n = 23), PSC (n = 24), and healthy controls (n = 11) were stained for TF by immunohistochemistry. Mononuclear cell contribution to TF expression was verified using flow cytometry.Results: TF was distributed at three distinct colonic locations: in subepithelial pericryptal sheath cells, in mononuclear cells, and in the intestinal stroma. In contrast to UC-where inflammation was accompanied with TF up-regulation-PSC-UC activity remained low during inflammation. Stromal TF positivity was found exclusively in ongoing inflammation.Conclusion: Our study provides additional support for a divergent pathogenesis in PSC-UC, with an inflammatory environment that differs from classical UC. Stromal TF emerges as a new marker of colonic inflammation.", "doi": "10.1080/03009734.2019.1689209", "pmid": "31774347", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC6968534"}], "notes": [], "created": "2026-08-21T12:04:34.626Z", "modified": "2026-08-21T12:04:34.966Z"}]}