{"entity": "researcher", "timestamp": "2026-09-27T17:00:27.251Z", "family": "Siddik", "given": "Abu Bakar", "initials": "AB", "orcid": "0000-0003-0037-7679", "affiliations": ["Division of Clinical Microbiology, Department of Laboratory Medicine, Karolinska Institutet, Huddinge, Stockholm, Sweden.", "Medical Microbiology Department, University of Manitoba, 727 McDermot Ave, Winnipeg, MB R3E 3P5, Canada."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/4b2b9b868a114cd2bd89c8cbc6f5af43.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/4b2b9b868a114cd2bd89c8cbc6f5af43"}}, "publications": [{"entity": "publication", "iuid": "245c914849964f56a1314c2de30f7e9f", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/245c914849964f56a1314c2de30f7e9f.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/245c914849964f56a1314c2de30f7e9f"}}, "title": "Phenotypic co-receptor tropism and Maraviroc sensitivity in HIV-1 subtype C from East Africa.", "authors": [{"family": "Siddik", "given": "Abu Bakar", "initials": "AB", "orcid": "0000-0003-0037-7679", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4b2b9b868a114cd2bd89c8cbc6f5af43.json"}}, {"family": "Haas", "given": "Alexandra", "initials": "A"}, {"family": "Rahman", "given": "Md Shanawazur", "initials": "MS"}, {"family": "Aralaguppe", "given": "Shambhu Ganeshappa", "initials": "SG"}, {"family": "Amogne", "given": "Wondwossen", "initials": "W"}, {"family": "Bader", "given": "Joelle", "initials": "J"}, {"family": "Klimkait", "given": "Thomas", "initials": "T"}, {"family": "Neogi", "given": "Ujjwal", "initials": "U", "orcid": "0000-0002-0844-3338", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/202f8d41f6694d67af0fd583b6b7f6e4.json"}}], "type": "comparative study", "published": "2018-02-05", "journal": {"title": "Sci Rep", "issn": "2045-2322", "volume": "8", "issue": "1", "pages": "2363", "issn-l": "2045-2322"}, "abstract": "Genotypic tropism testing (GTT) for co-receptor usage is a recommended tool for clinical practice before administration of the CCR5-antagonist maraviroc. For some isolates, phenotypic tropism testing (PTT) revealed discordant results with GTT. In this study, we performed a comparative study between GTT and PTT in HIV-1C from East Africa (HIV-1CEA) and compared the data with HIV-1B and 01_AE and described the maraviroc susceptibility in the CCR5-tropic strains. Patient-derived HIV-1 envgp120 region was cloned into a modified pNL4-3 plasmid expressing the luciferase gene. rPhenotyping dissected single clones from 31 HIV-1CEA infected patients and four strains with known phenotype. Additionally, 68 clones from 18 patients (HIV-1B: 5, 01_AE: 7, HIV-1CEA: 6) were used to determine the PTT in GHOST cell line. The respective V3-sequences were used for GTT. R5-tropic strains from HIV-1CEA (n = 20) and non-C (n = 12) were tested for maraviroc sensitivity in TZMbl cell line. The GTT falsely called a higher proportion of X4-tropic strains in HIV-1CET compared to PTT by both rPhenotyping and the GHOST-cell assay. When multiple clones were tested in a subset of patients' samples, both dual-tropic and R5-tropic strains were identified for HIV-1C. Relatively higher EC50 values were observed in HIV-1C strains than the non-C strains (p = 0.002).", "doi": "10.1038/s41598-018-20814-2", "pmid": "29403064", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC5799384"}, {"db": "pii", "key": "10.1038/s41598-018-20814-2"}], "notes": [], "created": "2018-12-05T12:23:58.234Z", "modified": "2026-09-23T12:06:14.199Z"}]}