{"entity": "researcher", "timestamp": "2026-08-20T20:40:33.957Z", "family": "Falk", "given": "Anna", "initials": "A", "orcid": "0000-0003-1634-8610", "affiliations": ["Neural Stem Cells, Department of Experimental Medical Science, Lund Stem Cell Center, Lund University, Lund, 221 84, Sweden.", "Department of Neuroscience, Karolinska Institutet, Solna, 171 65, Sweden."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/4a8825c33511418a83eceffdb530dadb.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/4a8825c33511418a83eceffdb530dadb"}}, "publications": [{"entity": "publication", "iuid": "6fa75e177c024da89b7fef0fa4426c5b", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/6fa75e177c024da89b7fef0fa4426c5b.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/6fa75e177c024da89b7fef0fa4426c5b"}}, "title": "On-Chip Neural Induction Boosts Neural Stem Cell Commitment: Toward a Pipeline for iPSC-Based Therapies.", "authors": [{"family": "Jain", "given": "Saumey", "initials": "S", "orcid": "0000-0002-2810-2151", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/969496e3da804b54bea1cf345294433f.json"}}, {"family": "Voulgaris", "given": "Dimitrios", "initials": "D", "orcid": "0000-0003-4574-1702", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c43e99f191f944d9a8ecf9363b80b87c.json"}}, {"family": "Thongkorn", "given": "Surangrat", "initials": "S", "orcid": "0000-0003-4149-9381", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a1cef026dbc14c9bb57ceebedbe517ee.json"}}, {"family": "Hesen", "given": "Rick", "initials": "R", "orcid": "0000-0003-2503-8139", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0a9fc8dbf8af48d1bc615f8cc313ad73.json"}}, {"family": "H\u00e4gg", "given": "Alice", "initials": "A", "orcid": "0000-0002-6022-8977", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/cd455c34f49d4bd68fb3ee542e57233b.json"}}, {"family": "Moslem", "given": "Mohsen", "initials": "M", "orcid": "0000-0002-2440-9586", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ac548b831c044a53ac324691083df9e1.json"}}, {"family": "Falk", "given": "Anna", "initials": "A", "orcid": "0000-0003-1634-8610", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4a8825c33511418a83eceffdb530dadb.json"}}, {"family": "Herland", "given": "Anna", "initials": "A", "orcid": "0000-0002-5002-2537", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/367ed0d139fe4136808b62ee61baa4f0.json"}}], "type": "journal article", "published": "2024-07-00", "journal": {"title": "Adv Sci (Weinh)", "issn": "2198-3844", "volume": "11", "issue": "25", "pages": "e2401859", "issn-l": null}, "abstract": "The clinical translation of induced pluripotent stem cells (iPSCs) holds great potential for personalized therapeutics. However, one of the main obstacles is that the current workflow to generate iPSCs is expensive, time-consuming, and requires standardization. A simplified and cost-effective microfluidic approach is presented for reprogramming fibroblasts into iPSCs and their subsequent differentiation into neural stem cells (NSCs). This method exploits microphysiological technology, providing a 100-fold reduction in reagents for reprogramming and a ninefold reduction in number of input cells. The iPSCs generated from microfluidic reprogramming of fibroblasts show upregulation of pluripotency markers and downregulation of fibroblast markers, on par with those reprogrammed in standard well-conditions. The NSCs differentiated in microfluidic chips show upregulation of neuroectodermal markers (ZIC1, PAX6, SOX1), highlighting their propensity for nervous system development. Cells obtained on conventional well plates and microfluidic chips are compared for reprogramming and neural induction by bulk RNA sequencing. Pathway enrichment analysis of NSCs from chip showed neural stem cell development enrichment and boosted commitment to neural stem cell lineage in initial phases of neural induction, attributed to a confined environment in a microfluidic chip. This method provides a cost-effective pipeline to reprogram and differentiate iPSCs for therapeutics compliant with current good manufacturing practices.", "doi": "10.1002/advs.202401859", "pmid": "38655836", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC11220685"}], "notes": [], "created": "2026-08-20T06:28:18.034Z", "modified": "2026-08-20T06:28:18.345Z"}, {"entity": "publication", "iuid": "9787117993cc48c69f47c081feaea41e", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/9787117993cc48c69f47c081feaea41e.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/9787117993cc48c69f47c081feaea41e"}}, "title": "Deficiency of the Heterogeneous Nuclear Ribonucleoprotein U locus leads to delayed hindbrain neurogenesis", "authors": [{"family": "Mastropasqua", "given": "Francesca", "initials": "F", "orcid": "0000-0003-4237-2446", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/082d532a452346ce94d507b9d8186ccb.json"}}, {"family": "Oksanen", "given": "Marika", "initials": "M", "orcid": "0000-0003-4140-4282", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8cc6d65911b84adab955eed1cb47dbe0.json"}}, {"family": "Soldini", "given": "Cristina", "initials": "C"}, {"family": "Alatar", "given": "Shemim", "initials": "S"}, {"family": "Arora", "given": "Abishek", "initials": "A", "orcid": "0000-0002-6149-4417", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ad35ae5b8ff64a6c965030e149a9749d.json"}}, {"family": "Ballarino", "given": "Roberto", "initials": "R", "orcid": "0000-0001-7812-0940", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/7885bdf463cf41538ea08a35898c1cae.json"}}, {"family": "Molinari", "given": "Maya", "initials": "M"}, {"family": "Agostini", "given": "Federico", "initials": "F", "orcid": "0000-0002-5453-2737", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/cc126b42b76c456b828eb2d86c7619e4.json"}}, {"family": "Poulet", "given": "Axel", "initials": "A", "orcid": "0000-0002-3415-8960", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5cdbdf8ec40043bca6f6157fbde8056d.json"}}, {"family": "Watts", "given": "Michelle", "initials": "M", "orcid": "0000-0002-3178-3429", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5f63c70eaec548e5aaa86e033f5d2207.json"}}, {"family": "Rabkina", "given": "Ielyzaveta", "initials": "I", "orcid": "0000-0001-5890-4115", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f70154226777443e80607201a988d234.json"}}, {"family": "Becker", "given": "Martin", "initials": "M", "orcid": "0000-0001-8442-4246", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/517c767452664ae4ba352a9702aebbfa.json"}}, {"family": "Li", "given": "Danyang", "initials": "D", "orcid": "0000-0001-7470-6645", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b954078f0053418fb07b876f41700177.json"}}, {"family": "Anderlid", "given": "Britt Marie", "initials": "BM", "orcid": "0000-0002-2488-6024", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8181592a5b6445a8873b412cb9830a32.json"}}, {"family": "Isaksson", "given": "Johan", "initials": "J", "orcid": "0000-0003-1033-2618", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/782d9f1c46694ba9b911d1ea6d7019f6.json"}}, {"family": "Remnelius", "given": "Karl Lundin", "initials": "KL"}, {"family": "Moslem", "given": "Mohsen", "initials": "M"}, {"family": "Jacob", "given": "Yannick", "initials": "Y", "orcid": "0000-0003-4741-0755", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b96e31cab421475b991a632f9491c51c.json"}}, {"family": "Falk", "given": "Anna", "initials": "A", "orcid": "0000-0003-1634-8610", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4a8825c33511418a83eceffdb530dadb.json"}}, {"family": "Crosetto", "given": "Nicola", "initials": "N", "orcid": "0000-0002-3019-6978", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0b1cef698ae749749b4017dbacb9e3db.json"}}, {"family": "Bienko", "given": "Magda", "initials": "M", "orcid": "0000-0002-6499-9082", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4b8da7a1f8524d0e812c83b1f5e20ef6.json"}}, {"family": "Santini", "given": "Emanuela", "initials": "E", "orcid": "0000-0002-8948-9652", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0871f60791434c2eb639cda18c586879.json"}}, {"family": "Borgkvist", "given": "Anders", "initials": "A", "orcid": "0000-0003-1698-0288", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d90df9c8aa584473b6e655c5463bbc88.json"}}, {"family": "B\u00f6lte", "given": "Sven", "initials": "S", "orcid": "0000-0002-4579-4970", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a274bd6d9c0f49789bedc115dd1356a3.json"}}, {"family": "Tammimies", "given": "Kristiina", "initials": "K", "orcid": "0000-0002-8324-4697", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/480cdbabfc57460f967e6921f12e6ec4.json"}}], "type": "posted-content", "published": "2022-09-17", "journal": {"issn-l": null}, "abstract": null, "doi": "10.1101/2022.09.14.507275", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T10:14:08.367Z", "modified": "2026-08-20T10:14:08.767Z"}, {"entity": "publication", "iuid": "f9884510a26a46399dbac644e459eefd", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/f9884510a26a46399dbac644e459eefd.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/f9884510a26a46399dbac644e459eefd"}}, "title": "Screening autism-associated environmental factors in differentiating human neural progenitors with fractional factorial design-based transcriptomics", "authors": [{"family": "Arora", "given": "Abishek", "initials": "A", "orcid": "0000-0002-6149-4417", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ad35ae5b8ff64a6c965030e149a9749d.json"}}, {"family": "Becker", "given": "Martin", "initials": "M", "orcid": "0000-0001-8442-4246", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/517c767452664ae4ba352a9702aebbfa.json"}}, {"family": "Marques", "given": "C\u00e1tia", "initials": "C"}, {"family": "Oksanen", "given": "Marika", "initials": "M", "orcid": "0000-0003-4140-4282", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8cc6d65911b84adab955eed1cb47dbe0.json"}}, {"family": "Li", "given": "Danyang", "initials": "D", "orcid": "0000-0001-7470-6645", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b954078f0053418fb07b876f41700177.json"}}, {"family": "Mastropasqua", "given": "Francesca", "initials": "F", "orcid": "0000-0003-4237-2446", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/082d532a452346ce94d507b9d8186ccb.json"}}, {"family": "Watts", "given": "Michelle Evelyn", "initials": "ME", "orcid": "0000-0002-3178-3429", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5f63c70eaec548e5aaa86e033f5d2207.json"}}, {"family": "Arora", "given": "Manish", "initials": "M", "orcid": "0000-0002-3350-4657", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/effd70cf5cd54ee09a99e21b00470617.json"}}, {"family": "Falk", "given": "Anna", "initials": "A", "orcid": "0000-0003-1634-8610", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4a8825c33511418a83eceffdb530dadb.json"}}, {"family": "Daub", "given": "Carsten Oliver", "initials": "CO", "orcid": "0000-0002-3295-8729", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/779af7484e984ad6883a105caa008313.json"}}, {"family": "Lanekoff", "given": "Ingela", "initials": "I", "orcid": "0000-0001-9040-3230", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/122c0ddd06d841fa9c86e3ee1b415cd2.json"}}, {"family": "Tammimies", "given": "Kristiina", "initials": "K", "orcid": "0000-0002-8324-4697", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/480cdbabfc57460f967e6921f12e6ec4.json"}}], "type": "posted-content", "published": "2022-06-28", "journal": {"issn-l": null}, "abstract": null, "doi": "10.1101/2022.06.27.497311", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T10:12:20.063Z", "modified": "2026-08-20T10:12:20.386Z"}, {"entity": "publication", "iuid": "292f06bd0df149b690a93382387a0fb8", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/292f06bd0df149b690a93382387a0fb8.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/292f06bd0df149b690a93382387a0fb8"}}, "title": "Broadly Active Antiviral Compounds Disturb Zika Virus Progeny Release Rescuing Virus-Induced Toxicity in Brain Organoids.", "authors": [{"family": "Pettke", "given": "Aleksandra", "initials": "A"}, {"family": "Tampere", "given": "Marianna", "initials": "M"}, {"family": "Pronk", "given": "Robin", "initials": "R", "orcid": "0000-0003-1831-588X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/022142621519497a8375ce5dc66493e5.json"}}, {"family": "Wallner", "given": "Olov", "initials": "O", "orcid": "0000-0002-6481-237X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3601a5a4d88f4452b6d1ac5f22c79d9f.json"}}, {"family": "Falk", "given": "Anna", "initials": "A", "orcid": "0000-0003-1634-8610", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4a8825c33511418a83eceffdb530dadb.json"}}, {"family": "Warpman Berglund", "given": "Ulrika", "initials": "U"}, {"family": "Helleday", "given": "Thomas", "initials": "T"}, {"family": "Mirazimi", "given": "Ali", "initials": "A"}, {"family": "Puumalainen", "given": "Marjo-Riitta", "initials": "MR", "orcid": "0000-0002-9936-9734", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3507a6b34e244ea9b20cbdf9731262ab.json"}}], "type": "journal article", "published": "2020-12-29", "journal": {"title": "Viruses", "issn": "1999-4915", "volume": "13", "issue": "1", "issn-l": "1999-4915"}, "abstract": "RNA viruses have gained plenty of attention during recent outbreaks of Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Zika virus (ZIKV), and Ebola virus. ZIKV is a vector borne Flavivirus that is spread by mosquitoes and it mainly infects neuronal progenitor cells. One hallmark of congenital ZIKV disease is a reduced brain size in fetuses, leading to severe neurological defects. The World Health Organization (WHO) is urging the development of new antiviral treatments against ZIKV, as there are no efficient countermeasures against ZIKV disease. Previously, we presented a new class of host-targeting antivirals active against a number of pathogenic RNA viruses, such as SARS-CoV-2. Here, we show the transfer of the image-based phenotypic antiviral assay to ZIKV-infected brain cells, followed by mechanism-of-action studies and a proof-of-concept study in a three-dimensional (3D) organoid model. The novel antiviral compounds showed a therapeutic window against ZIKV in several cell models and rescued ZIKV-induced neurotoxicity in brain organoids. The compound's mechanism-of-action was pinpointed to late steps in the virus life cycle, impairing the formation of new virus particles. Collectively, in this study, we expand the antiviral activity of new small molecule inhibitors to a new virus class of Flaviviruses, but also uncover compounds' mechanism of action, which are important for the further development of antivirals.", "doi": "10.3390/v13010037", "pmid": "33383826", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC7823652"}, {"db": "pii", "key": "v13010037"}], "notes": [], "created": "2026-08-20T13:43:19.574Z", "modified": "2026-08-20T13:43:19.704Z"}, {"entity": "publication", "iuid": "8fe4a1d767fc4ad7bc41eb58710cc1bd", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/8fe4a1d767fc4ad7bc41eb58710cc1bd.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/8fe4a1d767fc4ad7bc41eb58710cc1bd"}}, "title": "Modeling SHH-driven medulloblastoma with patient iPS cell-derived neural stem cells.", "authors": [{"family": "Susanto", "given": "Evelyn", "initials": "E"}, {"family": "Marin Navarro", "given": "Ana", "initials": "A"}, {"family": "Zhou", "given": "Leilei", "initials": "L"}, {"family": "Sundstr\u00f6m", "given": "Anders", "initials": "A", "orcid": "0000-0003-3942-6271", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/63e6602d2807417684239b93eb065a26.json"}}, {"family": "van Bree", "given": "Niek", "initials": "N", "orcid": "0000-0001-5010-3524", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/afdb3d47b0be46cbbf7e10f7367f6d7b.json"}}, {"family": "Stantic", "given": "Marina", "initials": "M"}, {"family": "Moslem", "given": "Mohsen", "initials": "M", "orcid": "0000-0002-2440-9586", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ac548b831c044a53ac324691083df9e1.json"}}, {"family": "Tailor", "given": "Jignesh", "initials": "J"}, {"family": "Rietdijk", "given": "Jonne", "initials": "J"}, {"family": "Zubillaga", "given": "Veronica", "initials": "V", "orcid": "0000-0002-8806-939X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0feb462e3293492bae0e051990b387df.json"}}, {"family": "H\u00fcbner", "given": "Jens-Martin", "initials": "JM"}, {"family": "Weishaupt", "given": "Holger", "initials": "H"}, {"family": "Wolfsberger", "given": "Johanna", "initials": "J"}, {"family": "Alafuzoff", "given": "Irina", "initials": "I", "orcid": "0000-0002-6249-569X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0d5a2e976bc84739a957f2b12517df7e.json"}}, {"family": "Nordgren", "given": "Ann", "initials": "A"}, {"family": "Magnaldo", "given": "Thierry", "initials": "T", "orcid": "0000-0001-9174-8599", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/09542eee2b7748769298e9d4fcdc5a22.json"}}, {"family": "Siesj\u00f6", "given": "Peter", "initials": "P"}, {"family": "Johnsen", "given": "John Inge", "initials": "JI", "orcid": "0000-0003-1277-812X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e63aa6bb0fba481abeb58c2f1568b3c9.json"}}, {"family": "Kool", "given": "Marcel", "initials": "M", "orcid": "0000-0002-6557-5468", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/da81ade40cfb45329b5ef3a190834913.json"}}, {"family": "Tammimies", "given": "Kristiina", "initials": "K", "orcid": "0000-0002-8324-4697", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/480cdbabfc57460f967e6921f12e6ec4.json"}}, {"family": "Darabi", "given": "Anna", "initials": "A", "orcid": "0000-0003-4326-8149", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/bb9f19a0c8694ee79ca515a79976d146.json"}}, {"family": "Swartling", "given": "Fredrik J", "initials": "FJ", "orcid": "0000-0002-8460-4367", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/2e673fa8c86348fb8c1a2ae34ba7ba9a.json"}}, {"family": "Falk", "given": "Anna", "initials": "A", "orcid": "0000-0003-1634-8610", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4a8825c33511418a83eceffdb530dadb.json"}}, {"family": "Wilhelm", "given": "Margareta", "initials": "M", "orcid": "0000-0002-0516-9724", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b22feedd1d3c464ba7d8f6635fb00826.json"}}], "type": "journal article", "published": "2020-08-18", "journal": {"title": "Proc. Natl. Acad. Sci. U.S.A.", "issn": "1091-6490", "volume": "117", "issue": "33", "pages": "20127-20138", "issn-l": "0027-8424"}, "abstract": "Medulloblastoma is the most common malignant brain tumor in children. Here we describe a medulloblastoma model using Induced pluripotent stem (iPS) cell-derived human neuroepithelial stem (NES) cells generated from a Gorlin syndrome patient carrying a germline mutation in the sonic hedgehog (SHH) receptor PTCH1. We found that Gorlin NES cells formed tumors in mouse cerebellum mimicking human medulloblastoma. Retransplantation of tumor-isolated NES (tNES) cells resulted in accelerated tumor formation, cells with reduced growth factor dependency, enhanced neurosphere formation in vitro, and increased sensitivity to Vismodegib. Using our model, we identified LGALS1 to be a GLI target gene that is up-regulated in both Gorlin tNES cells and SHH-subgroup of medulloblastoma patients. Taken together, we demonstrate that NES cells derived from Gorlin patients can be used as a resource to model medulloblastoma initiation and progression and to identify putative targets.", "doi": "10.1073/pnas.1920521117", "pmid": "32747535", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC7443968"}, {"db": "pii", "key": "1920521117"}], "notes": [], "created": "2026-08-20T09:30:35.661Z", "modified": "2026-08-20T09:30:36.075Z"}]}