{"entity": "researcher", "timestamp": "2026-09-25T18:00:35.473Z", "family": "Gao", "given": "Yang", "initials": "Y", "orcid": "0000-0002-7733-5365", "affiliations": ["Department of Neurobiology, Care Sciences and Society, Division of Neurogeriatrics, Karolinska Institutet, 171 64 Solna, Sweden."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/4a84fcad99bc4f99a5670f815338013b.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/4a84fcad99bc4f99a5670f815338013b"}}, "publications": [{"entity": "publication", "iuid": "0036d3ccde794e34856189cb1ba4ab65", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/0036d3ccde794e34856189cb1ba4ab65.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/0036d3ccde794e34856189cb1ba4ab65"}}, "title": "Live Cell FRET Imaging Reveals Amyloid \u03b2-Peptide Oligomerization in Hippocampal Neurons.", "authors": [{"family": "Gao", "given": "Yang", "initials": "Y", "orcid": "0000-0002-7733-5365", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4a84fcad99bc4f99a5670f815338013b.json"}}, {"family": "Wennmalm", "given": "Stefan", "initials": "S"}, {"family": "Winblad", "given": "Bengt", "initials": "B"}, {"family": "Schedin-Weiss", "given": "Sophia", "initials": "S"}, {"family": "Tjernberg", "given": "Lars O", "initials": "LO"}], "type": "journal article", "published": "2021-04-26", "journal": {"title": "Int J Mol Sci", "issn": "1422-0067", "volume": "22", "issue": "9", "issn-l": null}, "abstract": "Amyloid \u03b2-peptide (A\u03b2) oligomerization is believed to contribute to the neuronal dysfunction in Alzheimer disease (AD). Despite decades of research, many details of A\u03b2 oligomerization in neurons still need to be revealed. F\u00f6rster resonance energy transfer (FRET) is a simple but effective way to study molecular interactions. Here, we used a confocal microscope with a sensitive Airyscan detector for FRET detection. By live cell FRET imaging, we detected A\u03b242 oligomerization in primary neurons. The neurons were incubated with fluorescently labeled A\u03b242 in the cell culture medium for 24 h. A\u03b242 were internalized and oligomerized in the lysosomes/late endosomes in a concentration-dependent manner. Both the cellular uptake and intracellular oligomerization of A\u03b242 were significantly higher than for A\u03b240. These findings provide a better understanding of A\u03b242 oligomerization in neurons.", "doi": "10.3390/ijms22094530", "pmid": "33926107", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC8123703"}, {"db": "pii", "key": "ijms22094530"}], "notes": [], "created": "2026-09-23T12:13:14.816Z", "modified": "2026-09-23T12:13:14.905Z"}, {"entity": "publication", "iuid": "aceb6e65e4594c18b3fb39c04295a82d", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/aceb6e65e4594c18b3fb39c04295a82d.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/aceb6e65e4594c18b3fb39c04295a82d"}}, "title": "Live Cell FRET Imaging Reveals Amyloid \u03b2-Peptide Oligomerization in Hippocampal Neurons", "authors": [{"family": "Gao", "given": "Yang", "initials": "Y", "orcid": "0000-0002-7733-5365", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4a84fcad99bc4f99a5670f815338013b.json"}}, {"family": "Wennmalm", "given": "Stefan", "initials": "S"}, {"family": "Winblad", "given": "Bengt", "initials": "B"}, {"family": "Schedin-Weiss", "given": "Sophia", "initials": "S"}, {"family": "Tjernberg", "given": "Lars", "initials": "L"}], "type": "posted-content", "published": "2021-03-16", "journal": {"issn-l": null}, "abstract": null, "doi": "10.20944/preprints202103.0426.v1", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-09-23T14:31:50.806Z", "modified": "2026-09-23T14:31:50.839Z"}]}