{"entity": "researcher", "timestamp": "2026-08-20T20:36:27.694Z", "family": "Garcia-Pino", "given": "Abel", "initials": "A", "orcid": "0000-0002-0634-0300", "affiliations": ["Cellular and Molecular Microbiology, Facult\u00e9 des Sciences, Universit\u00e9 libre de Bruxelles (ULB), Brussels, Belgium. abel.garcia.pino@ulb.be."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/48acc9ebb8dd44668e3629de8dff9b93.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/48acc9ebb8dd44668e3629de8dff9b93"}}, "publications": [{"entity": "publication", "iuid": "0f4b3e4e4c164c13bb924a410748397b", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/0f4b3e4e4c164c13bb924a410748397b.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/0f4b3e4e4c164c13bb924a410748397b"}}, "title": "Nanobody-mediated modulation of long RSH enzymes Rel and RelA catalysis by restriction of their conformational landscape", "authors": [{"family": "Van Nerom", "given": "Katleen", "initials": "K", "orcid": "0000-0001-6430-4634", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/34ca8964c90449e59c63f0dc848f2748.json"}}, {"family": "Ainelo", "given": "Andres", "initials": "A", "orcid": "0000-0003-4073-3359", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/98431805ead04b05ade42ba75103efa8.json"}}, {"family": "Coppieters \u2019t Wallant", "given": "Kyo", "initials": "K", "orcid": "0000-0003-2358-999X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6129bd7b08684155a424e8bec6b24e56.json"}}, {"family": "Talavera-Perez", "given": "Ariel", "initials": "A", "orcid": "0000-0002-1865-5959", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b81a4477bdc945f782f9067806f4b0b3.json"}}, {"family": "Echemendia-Blanco", "given": "Dannele", "initials": "D", "orcid": "0009-0000-5590-9180", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/95ae4c1b993041abb05e11279a7628dc.json"}}, {"family": "Peeters", "given": "Sarah", "initials": "S"}, {"family": "El Khalfaoui Oulali", "given": "Brahim", "initials": "B"}, {"family": "Tamman", "given": "Hedvig", "initials": "H", "orcid": "0000-0003-4453-7814", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3cdb50e7133a4c4d867d4bb25f813f75.json"}}, {"family": "Kurata", "given": "Tatsuaki", "initials": "T", "orcid": "0000-0001-5986-7141", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/cd3ed90eb741422db2af62827e8947e2.json"}}, {"family": "Roghanian", "given": "Mohammad", "initials": "M", "orcid": "0000-0003-3116-3817", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0837cde3696a422cb624341ad856a7be.json"}}, {"family": "Martens", "given": "Chlo\u00e9", "initials": "C", "orcid": "0000-0002-1617-4238", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/2d740736340b4f62b7c19709310b5e14.json"}}, {"family": "Pardon", "given": "Els", "initials": "E", "orcid": "0000-0002-2466-0172", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a7d3f1115bd141acbe7a45df09f97ecd.json"}}, {"family": "Steyaert", "given": "Jan", "initials": "J", "orcid": "0000-0002-3825-874X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b634e567865547b4abe67fa569f96a87.json"}}, {"family": "Hauryliuk", "given": "Vasili", "initials": "V", "orcid": "0000-0003-2389-5057", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1884d638ebd34b34bcabc105ca2f4092.json"}}, {"family": "Garcia-Pino", "given": "Abel", "initials": "A", "orcid": "0000-0002-0634-0300", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/48acc9ebb8dd44668e3629de8dff9b93.json"}}], "type": "posted-content", "published": "2025-08-07", "journal": {"issn-l": null}, "abstract": null, "doi": "10.1101/2025.08.07.669093", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T11:06:47.758Z", "modified": "2026-08-20T11:06:48.149Z"}, {"entity": "publication", "iuid": "25cd7f5504364ef8a15a088efbc23866", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/25cd7f5504364ef8a15a088efbc23866.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/25cd7f5504364ef8a15a088efbc23866"}}, "title": "Mechanisms of neutralization of toxSAS from toxin-antitoxin modules.", "authors": [{"family": "Dominguez-Molina", "given": "Lucia", "initials": "L"}, {"family": "Kurata", "given": "Tatsuaki", "initials": "T"}, {"family": "Cepauskas", "given": "Albinas", "initials": "A"}, {"family": "Echemendia-Blanco", "given": "Dannele", "initials": "D"}, {"family": "Zedek", "given": "Safia", "initials": "S"}, {"family": "Talavera-Perez", "given": "Ariel", "initials": "A", "orcid": "0000-0002-1865-5959", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b81a4477bdc945f782f9067806f4b0b3.json"}}, {"family": "Atkinson", "given": "Gemma C", "initials": "GC", "orcid": "0000-0002-4861-4584", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f1879e0a722045769cfca0dd00d611a8.json"}}, {"family": "Hauryliuk", "given": "Vasili", "initials": "V", "orcid": "0000-0003-2389-5057", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1884d638ebd34b34bcabc105ca2f4092.json"}}, {"family": "Garcia-Pino", "given": "Abel", "initials": "A", "orcid": "0000-0002-0634-0300", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/48acc9ebb8dd44668e3629de8dff9b93.json"}}], "type": "journal article", "published": "2025-02-00", "journal": {"title": "Nat. Chem. Biol.", "issn": "1552-4469", "volume": "21", "issue": "2", "pages": "182-192", "issn-l": "1552-4450"}, "abstract": "Toxic small alarmone synthetase (toxSAS) enzymes constitute a family of bacterial effectors present in toxin-antitoxin and secretion systems. toxSASs act through either translation inhibition mediated by pyrophosphorylation of transfer RNA (tRNA) CCA ends or synthesis of the toxic alarmone adenosine pentaphosphate ((pp)pApp) and adenosine triphosphate (ATP) depletion, exemplified by FaRel2 and FaRel, respectively. However, structural bases of toxSAS neutralization are missing. Here we show that the pseudo-Zn2+ finger domain (pZFD) of the ATfaRel2 antitoxin precludes access of ATP to the pyrophosphate donor site of the FaRel2 toxin, without affecting recruitment of the tRNA pyrophosphate acceptor. By contrast, (pp)pApp-producing toxSASs are inhibited by Tis1 antitoxin domains though occlusion of the pyrophosphate acceptor-binding site. Consequently, the auxiliary pZFD of AT2faRel is dispensable for FaRel neutralization. Collectively, our study establishes the general principles of toxSAS inhibition by structured antitoxin domains, with the control strategy directly coupled to toxSAS substrate specificity.", "doi": "10.1038/s41589-024-01630-4", "pmid": "38834893", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC11782079"}, {"db": "pii", "key": "10.1038/s41589-024-01630-4"}], "notes": [], "created": "2026-08-20T09:01:35.683Z", "modified": "2026-08-20T09:01:35.803Z"}, {"entity": "publication", "iuid": "1102a74dd62944309e64d3922f7c28de", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/1102a74dd62944309e64d3922f7c28de.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/1102a74dd62944309e64d3922f7c28de"}}, "title": "Mechanism of phage sensing and abortion by toxin-antitoxin-chaperone systems", "authors": [{"family": "Mets", "given": "Toomas", "initials": "T", "orcid": "0000-0002-6796-7000", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/7dbceebe30234575b3575ef4f44c0511.json"}}, {"family": "Kurata", "given": "Tatsuaki", "initials": "T", "orcid": "0000-0001-5986-7141", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/cd3ed90eb741422db2af62827e8947e2.json"}}, {"family": "Ernits", "given": "Karin", "initials": "K", "orcid": "0000-0003-1072-4945", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/7f3dbd4a451c45878f71cb0b441c561d.json"}}, {"family": "Johansson", "given": "Marcus J O", "initials": "MJO", "orcid": "0000-0001-7117-7426", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/7ee1c60ebb934dfd996e67a32790db16.json"}}, {"family": "Craig", "given": "Sophie Z", "initials": "SZ"}, {"family": "Evora", "given": "Gabriel Medina", "initials": "GM"}, {"family": "Buttress", "given": "Jessica A", "initials": "JA", "orcid": "0000-0001-8822-6917", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0f7cee57e9d94c53b46aed566af418e7.json"}}, {"family": "Odai", "given": "Roni", "initials": "R"}, {"family": "Coppieters\u2018t Wallant", "given": "Kyo", "initials": "K", "orcid": "0000-0003-2358-999X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6129bd7b08684155a424e8bec6b24e56.json"}}, {"family": "Nakamoto", "given": "Jose A", "initials": "JA", "orcid": "0000-0001-8312-433X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/590d08398d614e9ca3307803cd263b8d.json"}}, {"family": "Shyrokova", "given": "Lena", "initials": "L"}, {"family": "Egorov", "given": "Artyom A", "initials": "AA", "orcid": "0000-0001-5578-5384", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/db620b86e13e45618e7cad6a06cd474e.json"}}, {"family": "Doering", "given": "Christopher Ross", "initials": "CR"}, {"family": "Brodiazhenko", "given": "Tetiana", "initials": "T", "orcid": "0000-0002-2591-9588", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8747a493473b4ebcabf9b1847be69e29.json"}}, {"family": "Laub", "given": "Michael T", "initials": "MT"}, {"family": "Tenson", "given": "Tanel", "initials": "T"}, {"family": "Strahl", "given": "Henrik", "initials": "H"}, {"family": "Martens", "given": "Chloe", "initials": "C"}, {"family": "Harms", "given": "Alexander", "initials": "A", "orcid": "0000-0003-2106-1286", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4c54ff7663e84b4388af871371448d4a.json"}}, {"family": "Garcia-Pino", "given": "Abel", "initials": "A", "orcid": "0000-0002-0634-0300", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/48acc9ebb8dd44668e3629de8dff9b93.json"}}, {"family": "Atkinson", "given": "Gemma C", "initials": "GC", "orcid": "0000-0002-4861-4584", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f1879e0a722045769cfca0dd00d611a8.json"}}, {"family": "Hauryliuk", "given": "Vasili", "initials": "V", "orcid": "0000-0003-2389-5057", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1884d638ebd34b34bcabc105ca2f4092.json"}}], "type": "posted-content", "published": "2024-02-24", "journal": {"issn-l": null}, "abstract": null, "doi": "10.1101/2024.02.24.581848", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T10:52:22.356Z", "modified": "2026-08-20T10:52:22.534Z"}]}