{"entity": "researcher", "timestamp": "2026-09-23T21:36:18.621Z", "family": "Ernfors", "given": "Patrik", "initials": "P", "orcid": "0000-0002-1140-3986", "affiliations": ["Department of Medical Biochemistry and Biophysics, Division of Molecular Neurobiology, Karolinska Institutet, 17177 Stockholm, Sweden."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/473c11a7cdea464d8d7f2b30f7d8e8d9.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/473c11a7cdea464d8d7f2b30f7d8e8d9"}}, "publications": [{"entity": "publication", "iuid": "8152f7c9aa8c44bb97fec9c3e843f07f", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/8152f7c9aa8c44bb97fec9c3e843f07f.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/8152f7c9aa8c44bb97fec9c3e843f07f"}}, "title": "Glioblastoma cytotoxicity conferred through dual disruption of endolysosomal homeostasis by Vacquinol-1.", "authors": [{"family": "Kwak", "given": "Dongoh", "initials": "D"}, {"family": "Hammarstr\u00f6m", "given": "Lars G J", "initials": "LGJ"}, {"family": "Haraldsson", "given": "Martin", "initials": "M"}, {"family": "Ernfors", "given": "Patrik", "initials": "P", "orcid": "0000-0002-1140-3986", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/473c11a7cdea464d8d7f2b30f7d8e8d9.json"}}], "type": "journal article", "published": "2021-10-15", "journal": {"title": "Neurooncol Adv", "issn": "2632-2498", "volume": "3", "issue": "1", "pages": "vdab152", "issn-l": null}, "abstract": "Increased membrane trafficking is observed in numerous cancer types, including glioblastoma. Targeting the oncogenic driven acquired alterations in membrane trafficking by synthetic cationic amphiphilic small molecules has recently been shown to induce death of glioblastoma cells, although the molecular targets are unknown.\n\nThe mechanism of action of the cationic amphiphilic drug Vacquinol-1 (Vacq1)-induced cytotoxicity was investigated using cell biology, biochemistry, functional experiments, chemical biology, unbiased antibody-based post-translation modification profiling, and mass spectrometry-based chemical proteomic analysis on patient-derived glioblastoma cells.\n\nVacq1 induced two types of abnormal endolysosomal vesicles, enlarged vacuoles and acidic vesicle organelles (AVOs). Mechanistically, enlarged vacuoles were formed by the impairment of lysosome reformation through the direct interaction and inhibition of calmodulin (CaM) by Vacq1, while AVO formation was induced by Vacq1 accumulation and acidification in the endosomal compartments through its activation of the v-ATPase. As a consequence of v-ATPase activation, cellular ATP consumption markedly increased, causing cellular energy shortage and cytotoxicity. This effect of Vacq1 was exacerbated by its inhibitory effects on calmodulin, causing lysosomal depletion and a failure of acidic vesicle organelle clearance.\n\nOur study identifies the targets of Vacq1 and the mechanisms underlying its selective cytotoxicity in glioblastoma cells. The dual function of Vacq1 sets in motion a glioblastoma-specific vicious cycle of ATP consumption resulting in cellular energy crisis and cell death.", "doi": "10.1093/noajnl/vdab152", "pmid": "34765974", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC8577523"}, {"db": "pii", "key": "vdab152"}], "notes": [], "created": "2026-09-23T13:39:46.977Z", "modified": "2026-09-23T13:39:47.034Z"}, {"entity": "publication", "iuid": "d3e5697230764d42808bc4ae4ec81c8f", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/d3e5697230764d42808bc4ae4ec81c8f.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/d3e5697230764d42808bc4ae4ec81c8f"}}, "title": "Spatiotemporal structure of cell fate decisions in murine neural crest.", "authors": [{"family": "Soldatov", "given": "Ruslan", "initials": "R", "orcid": "0000-0002-5670-5881", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/7e50dbca638d4ea9bd89a6cf9efa3e42.json"}}, {"family": "Kaucka", "given": "Marketa", "initials": "M", "orcid": "0000-0002-8781-9769", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a458c8d68ec049a287848d4051ff84e7.json"}}, {"family": "Kastriti", "given": "Maria Eleni", "initials": "ME", "orcid": "0000-0002-0563-7399", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9eb7a38e5c5c43efa5deccc7c3a89698.json"}}, {"family": "Petersen", "given": "Julian", "initials": "J", "orcid": "0000-0002-7444-0610", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b2df2dd8db04447fa78bf79bac1a17d2.json"}}, {"family": "Chontorotzea", "given": "Tatiana", "initials": "T", "orcid": "0000-0002-6134-1365", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6e1c562bad424dbcbc084c844b89b7bb.json"}}, {"family": "Englmaier", "given": "Lukas", "initials": "L", "orcid": "0000-0002-0082-2616", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/749ce625110d4f3b8839cbb733906219.json"}}, {"family": "Akkuratova", "given": "Natalia", "initials": "N", "orcid": "0000-0001-6733-8119", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/efaaf724dec64ecea77b388b4808b34f.json"}}, {"family": "Yang", "given": "Yunshi", "initials": "Y", "orcid": "0000-0003-0396-5257", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e3ad58da3aa94dadb3ca0199e186e92b.json"}}, {"family": "H\u00e4ring", "given": "Martin", "initials": "M", "orcid": "0000-0003-4949-0533", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/91efbb9dc7484fbf9b534b589ae7e78d.json"}}, {"family": "Dyachuk", "given": "Viacheslav", "initials": "V", "orcid": "0000-0001-8100-7367", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f5924dc2ded04e26a72ae6133dc9a4dd.json"}}, {"family": "Bock", "given": "Christoph", "initials": "C", "orcid": "0000-0001-6091-3088", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/78502e5803db472980b13c6f5d24f9e6.json"}}, {"family": "Farlik", "given": "Matthias", "initials": "M", "orcid": "0000-0003-0698-2992", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/52fc62860e274c03a1cbd529fc9e3cde.json"}}, {"family": "Piacentino", "given": "Michael L", "initials": "ML", "orcid": "0000-0003-1773-031X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d7117fd542bb491d8f7739c4fac0183e.json"}}, {"family": "Boismoreau", "given": "Franck", "initials": "F"}, {"family": "Hilscher", "given": "Markus M", "initials": "MM", "orcid": "0000-0001-7782-0830", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ec435b1759284c98aa17c7dfd11c3617.json"}}, {"family": "Yokota", "given": "Chika", "initials": "C"}, {"family": "Qian", "given": "Xiaoyan", "initials": "X", "orcid": "0000-0001-7509-8071", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/37300f2654d74a5c9b81dd287d47768a.json"}}, {"family": "Nilsson", "given": "Mats", "initials": "M"}, {"family": "Bronner", "given": "Marianne E", "initials": "ME", "orcid": "0000-0003-4274-1862", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f9b8c1d463cb487dafa5d18063b5108d.json"}}, {"family": "Croci", "given": "Laura", "initials": "L", "orcid": "0000-0002-7826-428X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/09f8e250d6ef4a77a35f3d4e99cf89e9.json"}}, {"family": "Hsiao", "given": "Wen-Yu", "initials": "WY"}, {"family": "Guertin", "given": "David A", "initials": "DA"}, {"family": "Brunet", "given": "Jean-Francois", "initials": "JF", "orcid": "0000-0002-1985-6103", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/bdc26ff742ce4c2386f6c50d2fa7a222.json"}}, {"family": "Consalez", "given": "Gian Giacomo", "initials": "GG", "orcid": "0000-0003-4594-6273", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b2996513cb9a4a68addab1ee25b9d777.json"}}, {"family": "Ernfors", "given": "Patrik", "initials": "P", "orcid": "0000-0002-1140-3986", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/473c11a7cdea464d8d7f2b30f7d8e8d9.json"}}, {"family": "Fried", "given": "Kaj", "initials": "K", "orcid": "0000-0002-9997-7078", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e105f61b4238430f8796cddde76e109e.json"}}, {"family": "Kharchenko", "given": "Peter V", "initials": "PV"}, {"family": "Adameyko", "given": "Igor", "initials": "I", "orcid": "0000-0001-5471-0356", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9991e2eddb0840dca291de18a9d77d13.json"}}], "type": "journal article", "published": "2019-06-07", "journal": {"title": "Science (New York, N.Y.)", "issn": "1095-9203", "volume": "364", "issue": "6444", "issn-l": "0036-8075"}, "abstract": "Neural crest cells are embryonic progenitors that generate numerous cell types in vertebrates. With single-cell analysis, we show that mouse trunk neural crest cells become biased toward neuronal lineages when they delaminate from the neural tube, whereas cranial neural crest cells acquire ectomesenchyme potential dependent on activation of the transcription factor Twist1. The choices that neural crest cells make to become sensory, glial, autonomic, or mesenchymal cells can be formalized as a series of sequential binary decisions. Each branch of the decision tree involves initial coactivation of bipotential properties followed by gradual shifts toward commitment. Competing fate programs are coactivated before cells acquire fate-specific phenotypic traits. Determination of a specific fate is achieved by increased synchronization of relevant programs and concurrent repression of competing fate programs.", "doi": "10.1126/science.aas9536", "pmid": "31171666", "labels": [], "xrefs": [{"db": "pii", "key": "364/6444/eaas9536"}], "notes": [], "created": "2026-09-23T06:32:18.236Z", "modified": "2026-09-23T06:32:18.858Z"}]}