{"entity": "researcher", "timestamp": "2026-08-23T12:20:04.535Z", "family": "Wrisberg", "given": "Marie-Kristin von", "initials": "MV", "orcid": "0000-0002-7100-4565", "affiliations": ["Center for Integrated Protein Science Munich (CIPSM), Department of Chemistry, Technical University of Munich, Institute for Advanced Study, Garching, 85748, Germany."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/403af701a74c405aa354146ab3e58ab9.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/403af701a74c405aa354146ab3e58ab9"}}, "publications": [{"entity": "publication", "iuid": "7d93482253c74fbf98cfde74f2b2d4ae", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/7d93482253c74fbf98cfde74f2b2d4ae.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/7d93482253c74fbf98cfde74f2b2d4ae"}}, "title": "Structural basis for VPS34 kinase activation by Rab1 and Rab5 on membranes.", "authors": [{"family": "Tremel", "given": "Shirley", "initials": "S", "orcid": "0000-0002-4077-0021", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/62440c46afba4e9a80eaefb5ef657680.json"}}, {"family": "Ohashi", "given": "Yohei", "initials": "Y", "orcid": "0000-0002-2288-130X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ff06a49ebb7e41099b458a428bf3fa76.json"}}, {"family": "Morado", "given": "Dustin R", "initials": "DR"}, {"family": "Bertram", "given": "Jessie", "initials": "J"}, {"family": "Perisic", "given": "Olga", "initials": "O", "orcid": "0000-0002-3842-2896", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/cddaff861d4d4280820ac0aebdbeaef0.json"}}, {"family": "Brandt", "given": "Laura T L", "initials": "LTL", "orcid": "0000-0003-1830-4128", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d804015aba8d4211bfe4b5e3b9c9e1d2.json"}}, {"family": "von Wrisberg", "given": "Marie-Kristin", "initials": "MK", "orcid": "0000-0002-7100-4565", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/403af701a74c405aa354146ab3e58ab9.json"}}, {"family": "Chen", "given": "Zhuo A", "initials": "ZA", "orcid": "0000-0003-0811-8348", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/39014f26ee5049649ac404fc8e15a550.json"}}, {"family": "Maslen", "given": "Sarah L", "initials": "SL", "orcid": "0000-0002-0261-2866", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9c9be103e68c4f619db8bfe162175c5a.json"}}, {"family": "Kovtun", "given": "Oleksiy", "initials": "O", "orcid": "0000-0001-6374-7863", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4ead6bd37aeb4c2b8716101956e17ae4.json"}}, {"family": "Skehel", "given": "Mark", "initials": "M", "orcid": "0000-0002-2432-0901", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0a5bf7dfb8e64258a4136260a1a9846a.json"}}, {"family": "Rappsilber", "given": "Juri", "initials": "J"}, {"family": "Lang", "given": "Kathrin", "initials": "K", "orcid": "0000-0002-1318-6567", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f7c04dc7c380470498ed008955868b97.json"}}, {"family": "Munro", "given": "Sean", "initials": "S", "orcid": "0000-0001-6160-5773", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/323cf9816130476d9c14df154b6ec6d8.json"}}, {"family": "Briggs", "given": "John A G", "initials": "JAG", "orcid": "0000-0003-3990-6910", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/702ee550a75346c7ac1cb4832bcf3aef.json"}}, {"family": "Williams", "given": "Roger L", "initials": "RL", "orcid": "0000-0001-7754-4207", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/32df58169da44be593c49b72db3889ae.json"}}], "type": "journal article", "published": "2021-03-10", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "12", "issue": "1", "pages": "1564", "issn-l": "2041-1723"}, "abstract": "The lipid phosphatidylinositol-3-phosphate (PI3P) is a regulator of two fundamental but distinct cellular processes, endocytosis and autophagy, so its generation needs to be under precise temporal and spatial control. PI3P is generated by two complexes that both contain the lipid kinase VPS34: complex II on endosomes (VPS34/VPS15/Beclin 1/UVRAG), and complex I on autophagosomes (VPS34/VPS15/Beclin 1/ATG14L). The endosomal GTPase Rab5 binds complex II, but the mechanism of VPS34 activation by Rab5 has remained elusive, and no GTPase is known to bind complex I. Here we show that Rab5a-GTP recruits endocytic complex II to membranes and activates it by binding between the VPS34 C2 and VPS15 WD40 domains. Electron cryotomography of complex II on Rab5a-decorated vesicles shows that the VPS34 kinase domain is released from inhibition by VPS15 and hovers over the lipid bilayer, poised for catalysis. We also show that the GTPase Rab1a, which is known to be involved in autophagy, recruits and activates the autophagy-specific complex I, but not complex II. Both Rabs bind to the same VPS34 interface but in a manner unique for each. These findings reveal how VPS34 complexes are activated on membranes by specific Rab GTPases and how they are recruited to unique cellular locations.", "doi": "10.1038/s41467-021-21695-2", "pmid": "33692360", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC7946940"}, {"db": "pii", "key": "10.1038/s41467-021-21695-2"}], "notes": [], "created": "2026-08-21T11:48:56.929Z", "modified": "2026-08-21T11:48:57.585Z"}, {"entity": "publication", "iuid": "eada2ce2db024ef2b7445d40b2481dcd", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/eada2ce2db024ef2b7445d40b2481dcd.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/eada2ce2db024ef2b7445d40b2481dcd"}}, "title": "Rab1-AMPylation by Legionella DrrA is allosterically activated by Rab1.", "authors": [{"family": "Du", "given": "Jiqing", "initials": "J", "orcid": "0000-0002-2940-3925", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/706d85fe86054072beb17874fc02c90e.json"}}, {"family": "Wrisberg", "given": "Marie-Kristin von", "initials": "MV", "orcid": "0000-0002-7100-4565", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/403af701a74c405aa354146ab3e58ab9.json"}}, {"family": "Gulen", "given": "Burak", "initials": "B", "orcid": "0000-0003-2945-3428", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6dc1ca7e458543a586b5d3cc5b15bf5b.json"}}, {"family": "Stahl", "given": "Matthias", "initials": "M", "orcid": "0000-0002-0176-9386", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e51ba23ba70a47bba2d5fa5de3610b2e.json"}}, {"family": "Pett", "given": "Christian", "initials": "C", "orcid": "0000-0001-7039-7312", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4f0d2a51c0fb4395816b7153932503b9.json"}}, {"family": "Hedberg", "given": "Christian", "initials": "C"}, {"family": "Lang", "given": "Kathrin", "initials": "K", "orcid": "0000-0002-1318-6567", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f7c04dc7c380470498ed008955868b97.json"}}, {"family": "Schneider", "given": "Sabine", "initials": "S", "orcid": "0000-0003-1054-8689", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6a735346a5ac4603b0f85f3b1112e996.json"}}, {"family": "Itzen", "given": "Aymelt", "initials": "A", "orcid": "0000-0002-4249-5617", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/831977b2fbac49269c0ada6144bc65dc.json"}}], "type": "journal article", "published": "2021-01-19", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "12", "issue": "1", "pages": "460", "issn-l": "2041-1723"}, "abstract": "Legionella pneumophila infects eukaryotic cells by forming a replicative organelle - the Legionella containing vacuole. During this process, the bacterial protein DrrA/SidM is secreted and manipulates the activity and post-translational modification (PTM) states of the vesicular trafficking regulator Rab1. As a result, Rab1 is modified with an adenosine monophosphate (AMP), and this process is referred to as AMPylation. Here, we use a chemical approach to stabilise low-affinity Rab:DrrA complexes in a site-specific manner to gain insight into the molecular basis of the interaction between the Rab protein and the AMPylation domain of DrrA. The crystal structure of the Rab:DrrA complex reveals a previously unknown non-conventional Rab-binding site (NC-RBS). Biochemical characterisation demonstrates allosteric stimulation of the AMPylation activity of DrrA via Rab binding to the NC-RBS. We speculate that allosteric control of DrrA could in principle prevent random and potentially cytotoxic AMPylation in the host, thereby perhaps ensuring efficient infection by Legionella.", "doi": "10.1038/s41467-020-20702-2", "pmid": "33469029", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC7815794"}, {"db": "pii", "key": "10.1038/s41467-020-20702-2"}], "notes": [], "created": "2026-08-21T11:48:54.410Z", "modified": "2026-08-21T11:48:54.806Z"}]}