{"entity": "researcher", "timestamp": "2026-05-12T21:28:50.786Z", "family": "Nilsson", "given": "Oscar", "initials": "O", "orcid": "0000-0003-2716-2563", "affiliations": ["Department of Chemistry and Molecular Biology, University of Gothenburg, 412 96, Gothenburg, Sweden."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/3e197da18897400e9babda511e0e31cb.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/3e197da18897400e9babda511e0e31cb"}}, "publications": [{"entity": "publication", "iuid": "52f86d470f684b3a8f157556912a95d1", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/52f86d470f684b3a8f157556912a95d1.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/52f86d470f684b3a8f157556912a95d1"}}, "title": "Serendipitous Identification of a Covalent Activator of Liver Pyruvate Kinase.", "authors": [{"family": "Battisti", "given": "Umberto Maria", "initials": "UM", "orcid": "0000-0002-1012-8644", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ede0c55885d34614a7c7bd4031366f58.json"}}, {"family": "Gao", "given": "Chunxia", "initials": "C"}, {"family": "Nilsson", "given": "Oscar", "initials": "O", "orcid": "0000-0003-2716-2563", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3e197da18897400e9babda511e0e31cb.json"}}, {"family": "Akladios", "given": "Fady", "initials": "F"}, {"family": "Lulla", "given": "Aleksei", "initials": "A"}, {"family": "Bogucka", "given": "Agnieszka", "initials": "A"}, {"family": "Nain-Perez", "given": "Amalyn", "initials": "A", "orcid": "0000-0002-0784-0446", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f9151d6839644baa9970e7fcd9cb9f29.json"}}, {"family": "H\u00e5versen", "given": "Liliana", "initials": "L", "orcid": "0000-0001-5180-7830", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5f13d4a790d14c408d4179ead9b13ae1.json"}}, {"family": "Kim", "given": "Woonghee", "initials": "W"}, {"family": "Boren", "given": "Jan", "initials": "J", "orcid": "0000-0003-0786-8091", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/73a770eb9e614448a046b8e9199052e9.json"}}, {"family": "Hyv\u00f6nen", "given": "Marko", "initials": "M", "orcid": "0000-0001-8683-4070", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9aad91f61bd84787a63a461c22808bf6.json"}}, {"family": "Uhlen", "given": "Mathias", "initials": "M", "orcid": "0000-0002-4858-8056", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9ea446aa574042a295d5f69437402f76.json"}}, {"family": "Mardinoglu", "given": "Adil", "initials": "A", "orcid": "0000-0002-4254-6090", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ca58bf2d214047e0ae5e38a42a0f2808.json"}}, {"family": "Gr\u00f8tli", "given": "Morten", "initials": "M", "orcid": "0000-0003-3621-4222", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f3d8708707ca48078f8113ecdeb9e6cb.json"}}], "type": "journal article", "published": "2023-01-03", "journal": {"title": "Chembiochem", "issn": "1439-7633", "volume": "24", "issue": "1", "pages": "e202200339", "issn-l": "1439-4227"}, "abstract": "Enzymes are effective biological catalysts that accelerate almost all metabolic reactions in living organisms. Synthetic modulators of enzymes are useful tools for the study of enzymatic reactions and can provide starting points for the design of new drugs. Here, we report on the discovery of a class of biologically active compounds that covalently modifies lysine residues in human liver pyruvate kinase (PKL), leading to allosteric activation of the enzyme (EC50 =0.29 \u03bcM). Surprisingly, the allosteric activation control point resides on the lysine residue K282 present in the catalytic site of PKL. These findings were confirmed by structural data, MS/MS experiments, and molecular modelling studies. Altogether, our study provides a molecular basis for the activation mechanism and establishes a framework for further development of human liver pyruvate kinase covalent activators.", "doi": "10.1002/cbic.202200339", "pmid": "36250581", "labels": {"Adil Mardinoglu": null, "SciLifeLab Fellow": null}, "xrefs": [{"db": "pmc", "key": "PMC10099687"}], "notes": [], "created": "2023-12-04T14:58:06.360Z", "modified": "2023-12-04T14:58:06.529Z"}, {"entity": "publication", "iuid": "3afb4cfd1e344d4cb4c1336796de29f1", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/3afb4cfd1e344d4cb4c1336796de29f1.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/3afb4cfd1e344d4cb4c1336796de29f1"}}, "title": "The Norway spruce genome sequence and conifer genome evolution.", "authors": [{"family": "Nystedt", "given": "Bj\u00f6rn", "initials": "B"}, {"family": "Street", "given": "Nathaniel R", "initials": "NR"}, {"family": "Wetterbom", "given": "Anna", "initials": "A"}, {"family": "Zuccolo", "given": "Andrea", "initials": "A"}, {"family": "Lin", "given": "Yao-Cheng", "initials": "YC"}, {"family": "Scofield", "given": "Douglas G", "initials": "DG", "orcid": "0000-0001-5235-6461", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6a2cc7a4bad04f5ab664027a374f5698.json"}}, {"family": "Vezzi", "given": "Francesco", "initials": "F"}, {"family": "Delhomme", "given": "Nicolas", "initials": "N"}, {"family": "Giacomello", "given": "Stefania", "initials": "S", "orcid": "0000-0003-0738-1574", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5a9c4a7494ba4284ab52158e6cbc85da.json"}}, {"family": "Alexeyenko", "given": "Andrey", "initials": "A", "orcid": "0000-0001-8812-6481", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8078f1505d9747e1ac76cf2f6d4ee606.json"}}, {"family": "Vicedomini", "given": "Riccardo", "initials": "R"}, {"family": "Sahlin", "given": "Kristoffer", "initials": "K", "orcid": "0000-0001-7378-2320", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ca3f1ebe1b9946a9ad9eb4b3058a911c.json"}}, {"family": "Sherwood", "given": "Ellen", "initials": "E"}, {"family": "Elfstrand", "given": "Malin", "initials": "M", "orcid": "0000-0002-0214-5284", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6ea896e8946240f587aecc990b6b8bfd.json"}}, {"family": "Gramzow", "given": "Lydia", "initials": "L"}, {"family": "Holmberg", "given": "Kristina", "initials": "K"}, {"family": "H\u00e4llman", "given": "Jimmie", "initials": "J"}, {"family": "Keech", "given": "Olivier", "initials": "O"}, {"family": "Klasson", "given": "Lisa", "initials": "L"}, {"family": "Koriabine", "given": "Maxim", "initials": "M"}, {"family": "Kucukoglu", "given": "Melis", "initials": "M"}, {"family": "K\u00e4ller", "given": "Max", "initials": "M"}, {"family": "Luthman", "given": "Johannes", "initials": "J"}, {"family": "Lysholm", "given": "Fredrik", "initials": "F"}, {"family": "Niittyl\u00e4", "given": "Totte", "initials": "T"}, {"family": "Olson", "given": "Ake", "initials": "A"}, {"family": "Rilakovic", "given": "Nemanja", "initials": "N"}, {"family": "Ritland", "given": "Carol", "initials": "C"}, {"family": "Rossell\u00f3", "given": "Josep A", "initials": "JA"}, {"family": "Sena", "given": "Juliana", "initials": "J"}, {"family": "Svensson", "given": "Thomas", "initials": "T"}, {"family": "Talavera-L\u00f3pez", "given": "Carlos", "initials": "C"}, {"family": "Thei\u00dfen", "given": "G\u00fcnter", "initials": "G"}, {"family": "Tuominen", "given": "Hannele", "initials": "H"}, {"family": "Vanneste", "given": "Kevin", "initials": "K"}, {"family": "Wu", "given": "Zhi-Qiang", "initials": "ZQ"}, {"family": "Zhang", "given": "Bo", "initials": "B", "orcid": "0000-0002-9199-1115", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/7ce4d3f145f14816a0c6f42481475c3a.json"}}, {"family": "Zerbe", "given": "Philipp", "initials": "P"}, {"family": "Arvestad", "given": "Lars", "initials": "L"}, {"family": "Bhalerao", "given": "Rishikesh", "initials": "R"}, {"family": "Bohlmann", "given": "Joerg", "initials": "J"}, {"family": "Bousquet", "given": "Jean", "initials": "J"}, {"family": "Garcia Gil", "given": "Rosario", "initials": "R"}, {"family": "Hvidsten", "given": "Torgeir R", "initials": "TR"}, {"family": "de Jong", "given": "Pieter", "initials": "P"}, {"family": "MacKay", "given": "John", "initials": "J"}, {"family": "Morgante", "given": "Michele", "initials": "M"}, {"family": "Ritland", "given": "Kermit", "initials": "K"}, {"family": "Sundberg", "given": "Bj\u00f6rn", "initials": "B"}, {"family": "Thompson", "given": "Stacey Lee", "initials": "SL"}, {"family": "Van de Peer", "given": "Yves", "initials": "Y"}, {"family": "Andersson", "given": "Bj\u00f6rn", "initials": "B", "orcid": "0000-0003-2975-9400", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/869d72720cd9462dba09cbc861556de6.json"}}, {"family": "Nilsson", "given": "Ove", "initials": "O", "orcid": "0000-0003-2716-2563", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3e197da18897400e9babda511e0e31cb.json"}}, {"family": "Ingvarsson", "given": "P\u00e4r K", "initials": "PK", "orcid": "0000-0001-9225-7521", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/fb09a6889bc64651ac6eb90b67edca3d.json"}}, {"family": "Lundeberg", "given": "Joakim", "initials": "J", "orcid": "0000-0003-4313-1601", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d9fa47767cd14ef2b9528c8b998cf095.json"}}, {"family": "Jansson", "given": "Stefan", "initials": "S"}], "type": "journal article", "published": "2013-05-30", "journal": {"title": "Nature", "issn": "1476-4687", "volume": "497", "issue": "7451", "pages": "579-584", "issn-l": "0028-0836"}, "abstract": "Conifers have dominated forests for more than 200 million years and are of huge ecological and economic importance. Here we present the draft assembly of the 20-gigabase genome of Norway spruce (Picea abies), the first available for any gymnosperm. The number of well-supported genes (28,354) is similar to the >100 times smaller genome of Arabidopsis thaliana, and there is no evidence of a recent whole-genome duplication in the gymnosperm lineage. Instead, the large genome size seems to result from the slow and steady accumulation of a diverse set of long-terminal repeat transposable elements, possibly owing to the lack of an efficient elimination mechanism. Comparative sequencing of Pinus sylvestris, Abies sibirica, Juniperus communis, Taxus baccata and Gnetum gnemon reveals that the transposable element diversity is shared among extant conifers. Expression of 24-nucleotide small RNAs, previously implicated in transposable element silencing, is tissue-specific and much lower than in other plants. We further identify numerous long (>10,000 base pairs) introns, gene-like fragments, uncharacterized long non-coding RNAs and short RNAs. This opens up new genomic avenues for conifer forestry and breeding.", "doi": "10.1038/nature12211", "pmid": "23698360", "labels": {"Affiliated researcher": null, "Kristoffer Sahlin": null, "SciLifeLab Fellow": null}, "xrefs": [{"db": "pii", "key": "nature12211"}], "notes": [], "created": "2018-12-05T08:19:22.709Z", "modified": "2025-11-04T14:23:20.985Z"}]}