{"entity": "researcher", "timestamp": "2026-07-20T21:45:16.211Z", "family": "Ernst", "given": "Christina", "initials": "C", "orcid": "0000-0002-3569-2209", "affiliations": [], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/37b4e062523b49108deea2308ebe66fe.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/37b4e062523b49108deea2308ebe66fe"}}, "publications": [{"entity": "publication", "iuid": "491465e04dbd421981e7fba893728b8b", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/491465e04dbd421981e7fba893728b8b.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/491465e04dbd421981e7fba893728b8b"}}, "title": "Direct Salmonella injection into enteroid cells allows the study of host-pathogen interactions in the cytosol with high spatiotemporal resolution.", "authors": [{"family": "Ernst", "given": "Chantal", "initials": "C", "orcid": "0000-0002-3569-2209", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/37b4e062523b49108deea2308ebe66fe.json"}}, {"family": "Andreassen", "given": "Patrick R", "initials": "PR"}, {"family": "Giger", "given": "Gabriel H", "initials": "GH"}, {"family": "Nguyen", "given": "Bidong D", "initials": "BD"}, {"family": "G\u00e4belein", "given": "Christoph G", "initials": "CG"}, {"family": "Guillaume-Gentil", "given": "Orane", "initials": "O"}, {"family": "Fattinger", "given": "Stefan A", "initials": "SA", "orcid": "0000-0003-4899-4414", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/2a3891756a0e46b7be8494556da9254f.json"}}, {"family": "Sellin", "given": "Mikael E", "initials": "ME", "orcid": "0000-0002-8355-0803", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/559c18ec87d64318a0afc6267ea879e2.json"}}, {"family": "Hardt", "given": "Wolf-Dietrich", "initials": "WD", "orcid": "0000-0002-9892-6420", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1049556a73b84d258e26a84382fe5870.json"}}, {"family": "Vorholt", "given": "Julia A", "initials": "JA", "orcid": "0000-0002-6011-4910", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/012ded54230347a6a3c023077f68db7c.json"}}], "type": "journal article", "published": "2024-04-00", "journal": {"title": "PLoS Biol.", "issn": "1545-7885", "volume": "22", "issue": "4", "pages": "e3002597", "issn-l": "1544-9173"}, "abstract": "Intestinal epithelial cells (IECs) play pivotal roles in nutrient uptake and in the protection against gut microorganisms. However, certain enteric pathogens, such as Salmonella enterica serovar Typhimurium (S. Tm), can invade IECs by employing flagella and type III secretion systems (T3SSs) with cognate effector proteins and exploit IECs as a replicative niche. Detection of flagella or T3SS proteins by IECs results in rapid host cell responses, i.e., the activation of inflammasomes. Here, we introduce a single-cell manipulation technology based on fluidic force microscopy (FluidFM) that enables direct bacteria delivery into the cytosol of single IECs within a murine enteroid monolayer. This approach allows to specifically study pathogen-host cell interactions in the cytosol uncoupled from preceding events such as docking, initiation of uptake, or vacuole escape. Consistent with current understanding, we show using a live-cell inflammasome reporter that exposure of the IEC cytosol to S. Tm induces NAIP/NLRC4 inflammasomes via its known ligands flagellin and T3SS rod and needle. Injected S. Tm mutants devoid of these invasion-relevant ligands were able to grow in the cytosol of IECs despite the absence of T3SS functions, suggesting that, in the absence of NAIP/NLRC4 inflammasome activation and the ensuing cell death, no effector-mediated host cell manipulation is required to render the epithelial cytosol growth-permissive for S. Tm. Overall, the experimental system to introduce S. Tm into single enteroid cells enables investigations into the molecular basis governing host-pathogen interactions in the cytosol with high spatiotemporal resolution.", "doi": "10.1371/journal.pbio.3002597", "pmid": "38684033", "labels": {"Mikael Sellin": null, "SciLifeLab Fellow": null}, "xrefs": [{"db": "pmc", "key": "PMC11057982"}, {"db": "pii", "key": "PBIOLOGY-D-23-02520"}], "notes": [], "created": "2024-11-25T16:24:24.849Z", "modified": "2025-04-08T06:11:18.094Z"}, {"entity": "publication", "iuid": "07bbde69b5114c3db56dad2f2b1efa1a", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/07bbde69b5114c3db56dad2f2b1efa1a.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/07bbde69b5114c3db56dad2f2b1efa1a"}}, "title": "The emergence of piRNAs against transposon invasion to preserve mammalian genome integrity.", "authors": [{"family": "Ernst", "given": "Christina", "initials": "C", "orcid": "0000-0002-3569-2209", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/37b4e062523b49108deea2308ebe66fe.json"}}, {"family": "Odom", "given": "Duncan T", "initials": "DT", "orcid": "0000-0001-6201-5599", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e8063d3396284b448f03300415fbdb9e.json"}}, {"family": "Kutter", "given": "Claudia", "initials": "C"}], "type": "journal article", "published": "2017-11-10", "journal": {"title": "Nat Commun", "issn": "2041-1723", "issn-l": "2041-1723", "volume": "8", "issue": "1", "pages": "1411"}, "abstract": "Transposable elements (TEs) contribute to the large amount of repetitive sequences in mammalian genomes and have been linked to species-specific genome innovations by rewiring regulatory circuitries. However, organisms need to restrict TE activity to ensure genome integrity, especially in germline cells to protect the transmission of genetic information to the next generation. This review features our current understandings of mammalian PIWI-interacting RNAs (piRNAs) and their role in TE regulation in spermatogenesis. Here we discuss functional implication and explore additional molecular mechanisms that inhibit transposon activity and altogether illustrate the paradoxical arms race between genome evolution and stability.", "doi": "10.1038/s41467-017-01049-7", "pmid": "29127279", "labels": {"Affiliated researcher": null, "Claudia Kutter": null, "SciLifeLab Fellow": null}, "xrefs": [{"db": "pii", "key": "10.1038/s41467-017-01049-7"}, {"db": "pmc", "key": "PMC5681665"}], "notes": [], "created": "2018-12-05T12:53:52.478Z", "modified": "2022-11-04T11:32:18.348Z"}, {"entity": "publication", "iuid": "5d01ecbd74f1446298ad02b7baf8b10f", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/5d01ecbd74f1446298ad02b7baf8b10f.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/5d01ecbd74f1446298ad02b7baf8b10f"}}, "title": "Successful transmission and transcriptional deployment of a human chromosome via mouse male meiosis.", "authors": [{"family": "Ernst", "given": "Christina", "initials": "C", "orcid": "0000-0002-3569-2209", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/37b4e062523b49108deea2308ebe66fe.json"}}, {"family": "Pike", "given": "Jeremy", "initials": "J"}, {"family": "Aitken", "given": "Sarah J", "initials": "SJ"}, {"family": "Long", "given": "Hannah K", "initials": "HK"}, {"family": "Eling", "given": "Nils", "initials": "N"}, {"family": "Stojic", "given": "Lovorka", "initials": "L"}, {"family": "Ward", "given": "Michelle C", "initials": "MC"}, {"family": "Connor", "given": "Frances", "initials": "F"}, {"family": "Rayner", "given": "Timothy F", "initials": "TF"}, {"family": "Lukk", "given": "Margus", "initials": "M"}, {"family": "Klose", "given": "Robert J", "initials": "RJ", "orcid": "0000-0002-8726-7888", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/964483b0fff64aa5a8cf355ee62397e6.json"}}, {"family": "Kutter", "given": "Claudia", "initials": "C", "orcid": "0000-0002-8047-0058", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a4c9dcde56304ab2a99bb1032e4ce834.json"}}, {"family": "Odom", "given": "Duncan T", "initials": "DT", "orcid": "0000-0001-6201-5599", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e8063d3396284b448f03300415fbdb9e.json"}}], "type": "journal article", "published": "2016-11-18", "journal": {"title": "Elife", "issn": "2050-084X", "issn-l": "2050-084X", "volume": "5", "issue": null, "pages": null}, "abstract": "Most human aneuploidies originate maternally, due in part to the presence of highly stringent checkpoints during male meiosis. Indeed, male sterility is common among aneuploid mice used to study chromosomal abnormalities, and male germline transmission of exogenous DNA has been rarely reported. Here we show that, despite aberrant testis architecture, males of the aneuploid Tc1 mouse strain produce viable sperm and transmit human chromosome 21 to create aneuploid offspring. In these offspring, we mapped transcription, transcriptional initiation, enhancer activity, non-methylated DNA, and transcription factor binding in adult tissues. Remarkably, when compared with mice derived from female passage of human chromosome 21, the chromatin condensation during spermatogenesis and the extensive epigenetic reprogramming specific to male germline transmission resulted in almost indistinguishable patterns of transcriptional deployment. Our results reveal an unexpected tolerance of aneuploidy during mammalian spermatogenesis, and the surprisingly robust ability of mouse developmental machinery to accurately deploy an exogenous chromosome, regardless of germline transmission.", "doi": "10.7554/eLife.20235", "pmid": "27855777", "labels": {"Affiliated researcher": null, "Claudia Kutter": null, "SciLifeLab Fellow": null}, "xrefs": [{"db": "pmc", "key": "PMC5161449"}], "notes": [], "created": "2018-12-03T14:39:09.294Z", "modified": "2022-11-04T11:32:19.206Z"}]}