{"entity": "researcher", "timestamp": "2026-08-20T20:45:15.540Z", "family": "Herland", "given": "Anna", "initials": "A", "orcid": "0000-0002-5002-2537", "affiliations": ["Division of Micro and Nanosystems, KTH Royal Institute of Technology, Stockholm, 100 44, Sweden.", "AIMES, Center for Integrated Medical and Engineering Science, Department of Neuroscience, Karolinska Institute, Solna, 171 65, Sweden.", "Division of Nanobiotechnology, Department of Protein Science, Science for Life Laboratory, KTH Royal Institute of Technology, Stockholm, 17165, Sweden."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/367ed0d139fe4136808b62ee61baa4f0.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/367ed0d139fe4136808b62ee61baa4f0"}}, "publications": [{"entity": "publication", "iuid": "09baeb2926c4435d9f51285afdea06bb", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/09baeb2926c4435d9f51285afdea06bb.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/09baeb2926c4435d9f51285afdea06bb"}}, "title": "Sub-5 nm Silicon Nanopore Sensors: Scalable Fabrication via Self-Limiting Metal-Assisted Chemical Etching.", "authors": [{"family": "De Ferrari", "given": "Fabio", "initials": "F", "orcid": "0000-0003-0960-9931", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/21be7bf528794d57b0b1431da2a95247.json"}}, {"family": "Raja", "given": "Shyamprasad N", "initials": "SN", "orcid": "0000-0002-2278-1368", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/58df34262ccf4569b54162cfd38525be.json"}}, {"family": "Herland", "given": "Anna", "initials": "A", "orcid": "0000-0002-5002-2537", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/367ed0d139fe4136808b62ee61baa4f0.json"}}, {"family": "Niklaus", "given": "Frank", "initials": "F", "orcid": "0000-0002-0525-8647", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/cbba7fd047e8468288fc79b7b0275ff3.json"}}, {"family": "Stemme", "given": "G\u00f6ran", "initials": "G", "orcid": "0000-0001-9552-4234", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/122f084eea624e5a801c8fafa6d48f6a.json"}}], "type": "journal article", "published": "2025-02-12", "journal": {"title": "ACS Appl Mater Interfaces", "issn": "1944-8252", "volume": "17", "issue": "6", "pages": "9047-9058", "issn-l": "1944-8244"}, "abstract": "Solid-state nanopores offer unique possibilities for biomolecule sensing; however, scalable production of sub-5 nm pores with precise diameter control remains a manufacturing challenge. In this work, we developed a scalable method to fabricate sub-5 nm nanopores in silicon (Si) nanomembranes through metal-assisted chemical etching (MACE) using gold nanoparticles. Notably, we present a previously unreported self-limiting effect that enables sub-5 nm nanopore formation from both 10 and 40 nm nanoparticles in the 12 nm thick monocrystalline device layer of a silicon-on-insulator substrate. This effect reveals distinctive etching dynamics in ultrathin Si nanomembranes, enabling precise control over nanopore dimensions. The resulting nanopore sensor, suspended over self-aligned spheroidal oxide undercuts with diameters of just a few hundred nanometers, exhibited low electrical noise and high stability due to encapsulation within dielectric layers. In DNA translocation experiments, our nanopore platform could distinguish folded and unfolded DNA conformations and maintained stable baseline conductance for up to 6 h, demonstrating both sensitivity and robustness. Our scalable nanopore fabrication method is compatible with wafer-level and batch processing and holds promise for advancing biomolecular sensing and analysis.", "doi": "10.1021/acsami.4c19750", "pmid": "39882662", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC11826499"}], "notes": [], "created": "2026-08-20T08:08:23.883Z", "modified": "2026-08-20T08:08:23.967Z"}, {"entity": "publication", "iuid": "7d836b85756546f1b18f2851412b8d7e", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/7d836b85756546f1b18f2851412b8d7e.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/7d836b85756546f1b18f2851412b8d7e"}}, "title": "Localized Nanopore Fabrication in Silicon Nitride Membranes by Femtosecond Laser Exposure and Subsequent Controlled Breakdown.", "authors": [{"family": "Leva", "given": "Chrysovalantou V", "initials": "CV"}, {"family": "Jain", "given": "Saumey", "initials": "S", "orcid": "0000-0002-2810-2151", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/969496e3da804b54bea1cf345294433f.json"}}, {"family": "Kistermann", "given": "Kevin", "initials": "K"}, {"family": "Sakurai", "given": "Kasumi", "initials": "K"}, {"family": "Stemme", "given": "G\u00f6ran", "initials": "G", "orcid": "0000-0001-9552-4234", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/122f084eea624e5a801c8fafa6d48f6a.json"}}, {"family": "Herland", "given": "Anna", "initials": "A", "orcid": "0000-0002-5002-2537", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/367ed0d139fe4136808b62ee61baa4f0.json"}}, {"family": "Mayer", "given": "Joachim", "initials": "J", "orcid": "0000-0003-3292-5342", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6b355f200cc346b389e9dc7901872fe7.json"}}, {"family": "Niklaus", "given": "Frank", "initials": "F", "orcid": "0000-0002-0525-8647", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/cbba7fd047e8468288fc79b7b0275ff3.json"}}, {"family": "Raja", "given": "Shyamprasad N", "initials": "SN", "orcid": "0000-0002-2278-1368", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/58df34262ccf4569b54162cfd38525be.json"}}], "type": "journal article", "published": "2025-02-05", "journal": {"title": "ACS Appl Mater Interfaces", "issn": "1944-8252", "volume": "17", "issue": "5", "pages": "8737-8748", "issn-l": "1944-8244"}, "abstract": "Controlled breakdown has emerged as an effective method for fabricating solid-state nanopores in thin suspended dielectric membranes for various biomolecular sensing applications. On an unpatterned membrane, the site of nanopore formation by controlled breakdown is random. Nanopore formation on a specific site on the membrane has previously been realized using local thinning of the membrane by lithographic processes or laser-assisted photothermal etching under immersion in an aqueous salt solution. However, these approaches require elaborate and expensive cleanroom-based lithography processes or involve intricate procedures using custom-made equipment. Here, we present a rapid cleanroom-free approach using single pulse femtosecond laser exposures of 50 nm thick silicon nitride membranes in air to localize the site of nanopore formation by subsequent controlled breakdown to an area less than 500 nm in diameter on the membrane. The precise positioning of the nanopores on the membrane could be produced both using laser exposure powers which caused significant thinning of the silicon nitride membrane (up to 60% of the original thickness locally), as well as at laser powers which caused no visible modification of the membrane at all. We show that nanopores made using our approach can work as single-molecule sensors by performing dsDNA translocation experiments. Due to the applicability of femtosecond laser processing to a wide range of membrane materials, we expect our approach to simplify the fabrication of localized nanopores by controlled breakdown in a variety of thin film material stacks, thereby enabling more sophisticated nanopore sensors.", "doi": "10.1021/acsami.5c00255", "pmid": "39870574", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC11803561"}], "notes": [], "created": "2026-08-20T08:08:29.746Z", "modified": "2026-08-20T08:08:29.834Z"}, {"entity": "publication", "iuid": "7ed185e75b5f4961928f78a60fa6a14c", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/7ed185e75b5f4961928f78a60fa6a14c.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/7ed185e75b5f4961928f78a60fa6a14c"}}, "title": "Human iPSC-based coculture model reveals neuroinflammatory crosstalk between microglia and astrocytes", "authors": [{"family": "Tujula", "given": "Iisa", "initials": "I", "orcid": "0000-0002-1271-6538", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6317ffd6cd844c13a73b2b4496004eb3.json"}}, {"family": "Hyv\u00e4rinen", "given": "Tanja", "initials": "T", "orcid": "0000-0002-8723-8817", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/781a941fb91d4506b2dfeff95457b5f5.json"}}, {"family": "Lotila", "given": "Johanna", "initials": "J", "orcid": "0000-0001-9352-4150", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/eb7dfe8e16fc41b29ac09ad14fa71e22.json"}}, {"family": "Rogal", "given": "Julia", "initials": "J", "orcid": "0000-0002-4315-5075", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/300bb6f28e184e5ab4a3bcfe9d57b222.json"}}, {"family": "Voulgaris", "given": "Dimitrios", "initials": "D", "orcid": "0000-0003-4574-1702", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c43e99f191f944d9a8ecf9363b80b87c.json"}}, {"family": "Sukki", "given": "Lassi", "initials": "L", "orcid": "0000-0001-9181-7355", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b0fce52fa07c49818e4d611d4dab097c.json"}}, {"family": "Tornberg", "given": "Kaisa", "initials": "K", "orcid": "0000-0002-7924-4520", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d4a92a593842490e9b1164e02456345f.json"}}, {"family": "Korpela", "given": "Katri", "initials": "K", "orcid": "0009-0003-8909-1606", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6937162b4b4849daa4841527e6eb6b9e.json"}}, {"family": "J\u00e4ntti", "given": "Henna", "initials": "H", "orcid": "0000-0003-3005-407X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6f9fd67b1f384905ad52137ac92c2c75.json"}}, {"family": "Malm", "given": "Tarja", "initials": "T", "orcid": "0000-0002-9530-7472", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a559f726359e4e1eb085ec4887c23187.json"}}, {"family": "Herland", "given": "Anna", "initials": "A", "orcid": "0000-0002-5002-2537", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/367ed0d139fe4136808b62ee61baa4f0.json"}}, {"family": "Kallio", "given": "Pasi", "initials": "P", "orcid": "0000-0002-2890-6782", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d51a1d33f2c347deba15f9cbc0a3e8b5.json"}}, {"family": "Narkilahti", "given": "Susanna", "initials": "S", "orcid": "0000-0002-7602-4418", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b880b769fccb457a9b6620a9b9fdd146.json"}}, {"family": "Hagman", "given": "Sanna", "initials": "S", "orcid": "0000-0002-3950-7736", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3e2b4b395e2646d38f32fcc13a2e8d0c.json"}}], "type": "posted-content", "published": "2025-01-03", "journal": {"issn-l": null}, "abstract": null, "doi": "10.1101/2025.01.03.628608", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T10:59:44.029Z", "modified": "2026-08-20T10:59:44.479Z"}, {"entity": "publication", "iuid": "db35fa2c43da4f12accee901dc796a29", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/db35fa2c43da4f12accee901dc796a29.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/db35fa2c43da4f12accee901dc796a29"}}, "title": "In Situ Functionalization of Polar Polythiophene-Based Organic Electrochemical Transistor to Interface In Vitro Models.", "authors": [{"family": "Buchmann", "given": "Sebastian", "initials": "S", "orcid": "0000-0001-7442-3020", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5d7fa19b55964898b15740e469f423a8.json"}}, {"family": "Stoop", "given": "Pepijn", "initials": "P"}, {"family": "Roekevisch", "given": "Kim", "initials": "K"}, {"family": "Jain", "given": "Saumey", "initials": "S", "orcid": "0000-0002-2810-2151", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/969496e3da804b54bea1cf345294433f.json"}}, {"family": "Kroon", "given": "Renee", "initials": "R", "orcid": "0000-0001-8053-4288", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8edb30ab10ad4bf3b9122d9ca6f6380d.json"}}, {"family": "M\u00fcller", "given": "Christian", "initials": "C", "orcid": "0000-0001-7859-7909", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c72e3ff85aa545c0a6ddec76712541db.json"}}, {"family": "Hamedi", "given": "Mahiar M", "initials": "MM", "orcid": "0000-0001-9088-1064", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ea65bd8c3fd642cbbd308e0641a0bc01.json"}}, {"family": "Zeglio", "given": "Erica", "initials": "E"}, {"family": "Herland", "given": "Anna", "initials": "A", "orcid": "0000-0002-5002-2537", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/367ed0d139fe4136808b62ee61baa4f0.json"}}], "type": "journal article", "published": "2024-10-09", "journal": {"title": "ACS Appl Mater Interfaces", "issn": "1944-8252", "volume": "16", "issue": "40", "pages": "54292-54303", "issn-l": "1944-8244"}, "abstract": "Organic mixed ionic-electronic conductors are promising materials for interfacing and monitoring biological systems, with the aim of overcoming current challenges based on the mismatch between biological materials and convectional inorganic conductors. The conjugated polymer/polyelectrolyte complex poly(3,4-ethylenedioxythiophene):polystyrenesulfonate (PEDOT/PSS) is, up to date, the most widely used polymer for in vitro or in vivo measurements in the field of organic bioelectronics. However, PEDOT/PSS organic electrochemical transistors (OECTs) are limited by depletion mode operation and lack chemical groups that enable synthetic modifications for biointerfacing. Recently introduced thiophene-based polymers with oligoether side chains can operate in accumulation mode, and their chemical structure can be tuned during synthesis, for example, by the introduction of hydroxylated side chains. Here, we introduce a new thiophene-based conjugated polymer, p(g42T-T)-8% OH, where 8% of the glycol side chains are functionalized with a hydroxyl group. We report for the first time the compatibility of conjugated polymers containing ethylene glycol side chains in direct contact with cells. The additional hydroxyl group allows covalent modification of the surface of polymer films, enabling fine-tuning of the surface interaction properties of p(g42T-T)-8% OH with biological materials, either hindering or promoting cell adhesion. We further use p(g42T-T)-8% OH to fabricate the OECTs and demonstrate for the first time the monitoring of epithelial barrier formation of Caco-2 cells in vitro using accumulation mode OECTs. The conjugated polymer p(g42T-T)-8% OH allows organic-electronic-based materials to be easily modified and optimized to interface and monitor biological systems.", "doi": "10.1021/acsami.4c09197", "pmid": "39327895", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC11472309"}], "notes": [], "created": "2026-08-20T08:08:22.084Z", "modified": "2026-08-20T08:08:22.221Z"}, {"entity": "publication", "iuid": "e154fdd25c074698958523cd700c4ae6", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/e154fdd25c074698958523cd700c4ae6.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/e154fdd25c074698958523cd700c4ae6"}}, "title": "Electromigrated Gold Nanogap Tunnel Junction Arrays: Fabrication and Electrical Behavior in Liquid and Gaseous Media.", "authors": [{"family": "Raja", "given": "Shyamprasad N", "initials": "SN", "orcid": "0000-0002-2278-1368", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/58df34262ccf4569b54162cfd38525be.json"}}, {"family": "Jain", "given": "Saumey", "initials": "S", "orcid": "0000-0002-2810-2151", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/969496e3da804b54bea1cf345294433f.json"}}, {"family": "Kipen", "given": "Javier", "initials": "J"}, {"family": "Jald\u00e9n", "given": "Joakim", "initials": "J"}, {"family": "Stemme", "given": "G\u00f6ran", "initials": "G", "orcid": "0000-0001-9552-4234", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/122f084eea624e5a801c8fafa6d48f6a.json"}}, {"family": "Herland", "given": "Anna", "initials": "A", "orcid": "0000-0002-5002-2537", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/367ed0d139fe4136808b62ee61baa4f0.json"}}, {"family": "Niklaus", "given": "Frank", "initials": "F", "orcid": "0000-0002-0525-8647", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/cbba7fd047e8468288fc79b7b0275ff3.json"}}], "type": "journal article", "published": "2024-07-17", "journal": {"title": "ACS Appl Mater Interfaces", "issn": "1944-8252", "volume": "16", "issue": "28", "pages": "37131-37146", "issn-l": "1944-8244"}, "abstract": "Tunnel junctions have been suggested as high-throughput electronic single molecule sensors in liquids with several seminal experiments conducted using break junctions with reconfigurable gaps. For practical single molecule sensing applications, arrays of on-chip integrated fixed-gap tunnel junctions that can be built into compact systems are preferable. Fabricating nanogaps by electromigration is one of the most promising approaches to realize on-chip integrated tunnel junction sensors. However, the electrical behavior of fixed-gap tunnel junctions immersed in liquid media has not been systematically studied to date, and the formation of electromigrated nanogap tunnel junctions in liquid media has not yet been demonstrated. In this work, we perform a comparative study of the formation and electrical behavior of arrays of gold nanogap tunnel junctions made by feedback-controlled electromigration immersed in various liquid and gaseous media (deionized water, mesitylene, ethanol, nitrogen, and air). We demonstrate that tunnel junctions can be obtained from microfabricated gold nanoconstrictions inside liquid media. Electromigration of junctions in air produces the highest yield (61-67%), electromigration in deionized water and mesitylene results in a lower yield than in air (44-48%), whereas electromigration in ethanol fails to produce viable tunnel junctions due to interfering electrochemical processes. We map out the stability of the conductance characteristics of the resulting tunnel junctions and identify medium-specific operational conditions that have an impact on the yield of forming stable junctions. Furthermore, we highlight the unique challenges associated with working with arrays of large numbers of tunnel junctions in batches. Our findings will inform future efforts to build single molecule sensors using on-chip integrated tunnel junctions.", "doi": "10.1021/acsami.4c03282", "pmid": "38954436", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC11261569"}], "notes": [], "created": "2026-08-20T08:08:20.105Z", "modified": "2026-08-20T08:08:20.211Z"}, {"entity": "publication", "iuid": "6fa75e177c024da89b7fef0fa4426c5b", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/6fa75e177c024da89b7fef0fa4426c5b.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/6fa75e177c024da89b7fef0fa4426c5b"}}, "title": "On-Chip Neural Induction Boosts Neural Stem Cell Commitment: Toward a Pipeline for iPSC-Based Therapies.", "authors": [{"family": "Jain", "given": "Saumey", "initials": "S", "orcid": "0000-0002-2810-2151", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/969496e3da804b54bea1cf345294433f.json"}}, {"family": "Voulgaris", "given": "Dimitrios", "initials": "D", "orcid": "0000-0003-4574-1702", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c43e99f191f944d9a8ecf9363b80b87c.json"}}, {"family": "Thongkorn", "given": "Surangrat", "initials": "S", "orcid": "0000-0003-4149-9381", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a1cef026dbc14c9bb57ceebedbe517ee.json"}}, {"family": "Hesen", "given": "Rick", "initials": "R", "orcid": "0000-0003-2503-8139", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0a9fc8dbf8af48d1bc615f8cc313ad73.json"}}, {"family": "H\u00e4gg", "given": "Alice", "initials": "A", "orcid": "0000-0002-6022-8977", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/cd455c34f49d4bd68fb3ee542e57233b.json"}}, {"family": "Moslem", "given": "Mohsen", "initials": "M", "orcid": "0000-0002-2440-9586", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ac548b831c044a53ac324691083df9e1.json"}}, {"family": "Falk", "given": "Anna", "initials": "A", "orcid": "0000-0003-1634-8610", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4a8825c33511418a83eceffdb530dadb.json"}}, {"family": "Herland", "given": "Anna", "initials": "A", "orcid": "0000-0002-5002-2537", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/367ed0d139fe4136808b62ee61baa4f0.json"}}], "type": "journal article", "published": "2024-07-00", "journal": {"title": "Adv Sci (Weinh)", "issn": "2198-3844", "volume": "11", "issue": "25", "pages": "e2401859", "issn-l": null}, "abstract": "The clinical translation of induced pluripotent stem cells (iPSCs) holds great potential for personalized therapeutics. However, one of the main obstacles is that the current workflow to generate iPSCs is expensive, time-consuming, and requires standardization. A simplified and cost-effective microfluidic approach is presented for reprogramming fibroblasts into iPSCs and their subsequent differentiation into neural stem cells (NSCs). This method exploits microphysiological technology, providing a 100-fold reduction in reagents for reprogramming and a ninefold reduction in number of input cells. The iPSCs generated from microfluidic reprogramming of fibroblasts show upregulation of pluripotency markers and downregulation of fibroblast markers, on par with those reprogrammed in standard well-conditions. The NSCs differentiated in microfluidic chips show upregulation of neuroectodermal markers (ZIC1, PAX6, SOX1), highlighting their propensity for nervous system development. Cells obtained on conventional well plates and microfluidic chips are compared for reprogramming and neural induction by bulk RNA sequencing. Pathway enrichment analysis of NSCs from chip showed neural stem cell development enrichment and boosted commitment to neural stem cell lineage in initial phases of neural induction, attributed to a confined environment in a microfluidic chip. This method provides a cost-effective pipeline to reprogram and differentiate iPSCs for therapeutics compliant with current good manufacturing practices.", "doi": "10.1002/advs.202401859", "pmid": "38655836", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC11220685"}], "notes": [], "created": "2026-08-20T06:28:18.034Z", "modified": "2026-08-20T06:28:18.345Z"}, {"entity": "publication", "iuid": "afae2549375b4f92939156b7e6029fe2", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/afae2549375b4f92939156b7e6029fe2.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/afae2549375b4f92939156b7e6029fe2"}}, "title": "Cleanroom-Free Direct Laser Micropatterning of Polymers for Organic Electrochemical Transistors in Logic Circuits and Glucose Biosensors.", "authors": [{"family": "Enrico", "given": "Alessandro", "initials": "A", "orcid": "0000-0002-8821-6759", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/637ef7e9c6a14e4597b4304013c988b7.json"}}, {"family": "Buchmann", "given": "Sebastian", "initials": "S", "orcid": "0000-0001-7442-3020", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5d7fa19b55964898b15740e469f423a8.json"}}, {"family": "De Ferrari", "given": "Fabio", "initials": "F", "orcid": "0000-0003-0960-9931", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/21be7bf528794d57b0b1431da2a95247.json"}}, {"family": "Lin", "given": "Yunfan", "initials": "Y", "orcid": "0000-0002-6560-5209", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e0a9d5ca176d42eb958ae277ed28062f.json"}}, {"family": "Wang", "given": "Yazhou", "initials": "Y"}, {"family": "Yue", "given": "Wan", "initials": "W"}, {"family": "M\u00e5rtensson", "given": "Gustaf", "initials": "G", "orcid": "0000-0002-6041-4980", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/77d0e449fc994885908cf8c228ce2995.json"}}, {"family": "Stemme", "given": "G\u00f6ran", "initials": "G", "orcid": "0000-0001-9552-4234", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/122f084eea624e5a801c8fafa6d48f6a.json"}}, {"family": "Hamedi", "given": "Mahiar Max", "initials": "MM", "orcid": "0000-0001-9088-1064", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ea65bd8c3fd642cbbd308e0641a0bc01.json"}}, {"family": "Niklaus", "given": "Frank", "initials": "F", "orcid": "0000-0002-0525-8647", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/cbba7fd047e8468288fc79b7b0275ff3.json"}}, {"family": "Herland", "given": "Anna", "initials": "A", "orcid": "0000-0002-5002-2537", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/367ed0d139fe4136808b62ee61baa4f0.json"}}, {"family": "Zeglio", "given": "Erica", "initials": "E", "orcid": "0000-0002-6428-0633", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e9f9fe3dcb5e4c9891c6f463c32a1679.json"}}], "type": "journal article", "published": "2024-07-00", "journal": {"title": "Adv Sci (Weinh)", "issn": "2198-3844", "volume": "11", "issue": "27", "pages": "e2307042", "issn-l": null}, "abstract": "Organic electrochemical transistors (OECTs) are promising devices for bioelectronics, such as biosensors. However, current cleanroom-based microfabrication of OECTs hinders fast prototyping and widespread adoption of this technology for low-volume, low-cost applications. To address this limitation, a versatile and scalable approach for ultrafast laser microfabrication of OECTs is herein reported, where a femtosecond laser to pattern insulating polymers (such as parylene C or polyimide) is first used, exposing the underlying metal electrodes serving as transistor terminals (source, drain, or gate). After the first patterning step, conducting polymers, such as poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate) (PEDOT:PSS), or semiconducting polymers, are spin-coated on the device surface. Another femtosecond laser patterning step subsequently defines the active polymer area contributing to the OECT performance by disconnecting the channel and gate from the surrounding spin-coated film. The effective OECT width can be defined with high resolution (down to 2 \u00b5m) in less than a second of exposure. Micropatterning the OECT channel area significantly improved the transistor switching performance in the case of PEDOT:PSS-based transistors, speeding up the devices by two orders of magnitude. The utility of this OECT manufacturing approach is demonstrated by fabricating complementary logic (inverters) and glucose biosensors, thereby showing its potential to accelerate OECT research.", "doi": "10.1002/advs.202307042", "pmid": "38225700", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC11251563"}], "notes": [], "created": "2026-08-20T06:28:15.482Z", "modified": "2026-08-20T06:28:15.911Z"}, {"entity": "publication", "iuid": "7aa4e0e5a6884343ac332aa6b30ac772", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/7aa4e0e5a6884343ac332aa6b30ac772.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/7aa4e0e5a6884343ac332aa6b30ac772"}}, "title": "Human In Vitro Models of Neuroenergetics and Neurometabolic Disturbances: Current Advances and Clinical Perspectives.", "authors": [{"family": "Rogal", "given": "Julia", "initials": "J", "orcid": "0000-0002-4315-5075", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/300bb6f28e184e5ab4a3bcfe9d57b222.json"}}, {"family": "Zamproni", "given": "Laura Nicoleti", "initials": "LN"}, {"family": "Nikolakopoulou", "given": "Polyxeni", "initials": "P"}, {"family": "Ygberg", "given": "Sofia", "initials": "S"}, {"family": "Wedell", "given": "Anna", "initials": "A"}, {"family": "Wredenberg", "given": "Anna", "initials": "A", "orcid": "0000-0002-2500-6121", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1bd991602b184ab48a4dca93b7901466.json"}}, {"family": "Herland", "given": "Anna", "initials": "A", "orcid": "0000-0002-5002-2537", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/367ed0d139fe4136808b62ee61baa4f0.json"}}], "type": "journal article", "published": "2024-06-14", "journal": {"title": "Stem Cells Transl Med", "issn": "2157-6580", "volume": "13", "issue": "6", "pages": "505-514", "issn-l": "2157-6564"}, "abstract": "Neurological conditions conquer the world; they are the leading cause of disability and the second leading cause of death worldwide, and they appear all around the world in every age group, gender, nationality, and socioeconomic class. Despite the growing evidence of an immense impact of perturbations in neuroenergetics on overall brain function, only little is known about the underlying mechanisms. Especially human insights are sparse, owing to a shortage of physiologically relevant model systems. With this perspective, we aim to explore the key steps and considerations involved in developing an advanced human in vitro model for studying neuroenergetics. We discuss biological and technological strategies to meet the requirements of a predictive model, aiming at providing a guide and inspiration for future in vitro models of neuroenergetics.", "doi": "10.1093/stcltm/szae021", "pmid": "38588471", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC11165162"}, {"db": "pii", "key": "7642385"}], "notes": [], "created": "2026-08-20T09:51:50.307Z", "modified": "2026-08-20T09:51:50.467Z"}, {"entity": "publication", "iuid": "d0787480eb844c68a8304e4a05170e87", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/d0787480eb844c68a8304e4a05170e87.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/d0787480eb844c68a8304e4a05170e87"}}, "title": "Mixing Insulating Commodity Polymers with Semiconducting n-type Polymers Enables High-Performance Electrochemical Transistors.", "authors": [{"family": "Zeglio", "given": "Erica", "initials": "E", "orcid": "0000-0002-6428-0633", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e9f9fe3dcb5e4c9891c6f463c32a1679.json"}}, {"family": "Wang", "given": "Yazhou", "initials": "Y"}, {"family": "Jain", "given": "Saumey", "initials": "S", "orcid": "0000-0002-2810-2151", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/969496e3da804b54bea1cf345294433f.json"}}, {"family": "Lin", "given": "Yunfan", "initials": "Y"}, {"family": "Avila Ramirez", "given": "Alan Eduardo", "initials": "AE", "orcid": "0000-0001-9549-1516", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/900a9e8a82fd4708b025f91c613428fe.json"}}, {"family": "Feng", "given": "Kui", "initials": "K"}, {"family": "Guo", "given": "Xugang", "initials": "X"}, {"family": "Ose", "given": "Helena", "initials": "H", "orcid": "0009-0008-5546-4095", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8eb950bfbdc4441b83d6cbd43bc2a8f4.json"}}, {"family": "Mozolevskis", "given": "Gatis", "initials": "G", "orcid": "0000-0001-7418-9123", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/59ff8fe8b6d74ecaab2998c57ad69032.json"}}, {"family": "Mawad", "given": "Damia", "initials": "D"}, {"family": "Yue", "given": "Wan", "initials": "W"}, {"family": "Hamedi", "given": "Mahiar Max", "initials": "MM"}, {"family": "Herland", "given": "Anna", "initials": "A", "orcid": "0000-0002-5002-2537", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/367ed0d139fe4136808b62ee61baa4f0.json"}}], "type": "journal article", "published": "2024-06-00", "journal": {"title": "Adv. Mater. Weinheim", "issn": "1521-4095", "volume": "36", "issue": "23", "pages": "e2302624", "issn-l": "0935-9648"}, "abstract": "Diluting organic semiconductors with a host insulating polymer is used to increase the electronic mobility in organic electronic devices, such as thin film transistors, while considerably reducing material costs. In contrast to organic electronics, bioelectronic devices such as the organic electrochemical transistor (OECT) rely on both electronic and ionic mobility for efficient operation, making it challenging to integrate hydrophobic polymers as the predominant blend component. This work shows that diluting the n-type conjugated polymer p(N-T) with high molecular weight polystyrene (10 KDa) leads to OECTs with over three times better mobility-volumetric capacitance product (\u00b5C*) with respect to the pristine p(N-T) (from 4.3 to 13.4 F V-1 cm-1 s-1) while drastically decreasing the amount of conjugated polymer (six times less). This improvement in \u00b5C* is due to a dramatic increase in electronic mobility by two orders of magnitude, from 0.059 to 1.3 cm2 V-1 s-1 for p(N-T):Polystyrene 10 KDa 1:6. Moreover, devices made with this polymer blend show better stability, retaining 77% of the initial drain current after 60 minutes operation in contrast to 12% for pristine p(N-T). These results open a new generation of low-cost organic mixed ionic-electronic conductors where the bulk of the film is made by a commodity polymer.", "doi": "10.1002/adma.202302624", "pmid": "38431796", "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T06:27:58.514Z", "modified": "2026-08-20T06:27:58.783Z"}, {"entity": "publication", "iuid": "754b9399b4a14490b9326e82661cbc9f", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/754b9399b4a14490b9326e82661cbc9f.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/754b9399b4a14490b9326e82661cbc9f"}}, "title": "Astrocyte 3D culture and bioprinting using peptide functionalized hyaluronan hydrogels.", "authors": [{"family": "Matthiesen", "given": "Isabelle", "initials": "I", "orcid": "0000-0003-4787-7785", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f57ebf85ca0d4d279b4852fd66f05251.json"}}, {"family": "Jury", "given": "Michael", "initials": "M", "orcid": "0000-0002-2803-2237", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/788e0c74b5874bf18585debca9bcae79.json"}}, {"family": "Rasti Boroojeni", "given": "Fatemeh", "initials": "F", "orcid": "0000-0002-3346-4755", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/71244257d00b4117b1b7e67b4ebba989.json"}}, {"family": "Ludwig", "given": "Saskia L", "initials": "SL"}, {"family": "Holzreuter", "given": "Muriel", "initials": "M", "orcid": "0000-0002-7580-3049", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/07143f4b91674a28a565da46ed4441f4.json"}}, {"family": "Buchmann", "given": "Sebastian", "initials": "S", "orcid": "0000-0001-7442-3020", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5d7fa19b55964898b15740e469f423a8.json"}}, {"family": "\u00c5man Tr\u00e4ger", "given": "Andrea", "initials": "A", "orcid": "0000-0001-6180-8917", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5de952fb4f584849a7567537d861de0a.json"}}, {"family": "Seleg\u00e5rd", "given": "Robert", "initials": "R", "orcid": "0000-0002-1781-1489", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1cb5b515710f4a77ab1fa32005577093.json"}}, {"family": "Winkler", "given": "Thomas E", "initials": "TE", "orcid": "0000-0002-2331-4833", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/851879d753574f5a80716e94dcc24590.json"}}, {"family": "Aili", "given": "Daniel", "initials": "D", "orcid": "0000-0002-7001-9415", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3624d34bc7734e329c0d4b465b5ac567.json"}}, {"family": "Herland", "given": "Anna", "initials": "A", "orcid": "0000-0002-5002-2537", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/367ed0d139fe4136808b62ee61baa4f0.json"}}], "type": "journal article", "published": "2023-01-30", "journal": {"title": "Sci Technol Adv Mater", "issn": "1468-6996", "volume": "24", "issue": "1", "pages": "2165871", "issn-l": null}, "abstract": "Astrocytes play an important role in the central nervous system, contributing to the development of and maintenance of synapses, recycling of neurotransmitters, and the integrity and function of the blood-brain barrier. Astrocytes are also linked to the pathophysiology of various neurodegenerative diseases. Astrocyte function and organization are tightly regulated by interactions mediated by the extracellular matrix (ECM). Engineered hydrogels can mimic key aspects of the ECM and can allow for systematic studies of ECM-related factors that govern astrocyte behaviour. In this study, we explore the interactions between neuroblastoma (SH-SY5Y) and glioblastoma (U87) cell lines and human fetal primary astrocytes (FPA) with a modular hyaluronan-based hydrogel system. Morphological analysis reveals that FPA have a higher degree of interactions with the hyaluronan-based gels compared to the cell lines. This interaction is enhanced by conjugation of cell-adhesion peptides (cRGD and IKVAV) to the hyaluronan backbone. These effects are retained and pronounced in 3D bioprinted structures. Bioprinted FPA using cRGD functionalized hyaluronan show extensive and defined protrusions and multiple connections between neighboring cells. Possibilities to tailor and optimize astrocyte-compatible ECM-mimicking hydrogels that can be processed by means of additive biofabrication can facilitate the development of advanced tissue and disease models of the central nervous system.", "doi": "10.1080/14686996.2023.2165871", "pmid": "36733710", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC9888471"}, {"db": "pii", "key": "2165871"}], "notes": [], "created": "2026-08-20T09:35:23.675Z", "modified": "2026-08-20T09:35:23.876Z"}, {"entity": "publication", "iuid": "8f0f1fbb1ca1447a89c7a627fb4546e4", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/8f0f1fbb1ca1447a89c7a627fb4546e4.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/8f0f1fbb1ca1447a89c7a627fb4546e4"}}, "title": "n-Type Organic Electrochemical Transistors with High Transconductance and Stability", "authors": [{"family": "Wang", "given": "Yazhou", "initials": "Y"}, {"family": "Zhu", "given": "Genming", "initials": "G"}, {"family": "Zeglio", "given": "Erica", "initials": "E"}, {"family": "Castillo", "given": "Tania Cecilia Hidalgo", "initials": "TCH", "orcid": "0000-0001-5299-9539", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c5676572026b4349905045ea4bbcc431.json"}}, {"family": "Haseena", "given": "Sheik", "initials": "S"}, {"family": "Ravva", "given": "Mahesh Kumar", "initials": "MK", "orcid": "0000-0001-9619-0176", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ea8a4a8c53b7426ab95e0e3be00a026c.json"}}, {"family": "Cong", "given": "Shengyu", "initials": "S"}, {"family": "Chen", "given": "Junxin", "initials": "J"}, {"family": "Lan", "given": "Liuyuan", "initials": "L"}, {"family": "Li", "given": "Zhengke", "initials": "Z"}, {"family": "Herland", "given": "Anna", "initials": "A", "orcid": "0000-0002-5002-2537", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/367ed0d139fe4136808b62ee61baa4f0.json"}}, {"family": "McCulloch", "given": "Iain", "initials": "I", "orcid": "0000-0002-6340-7217", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/08a46bacf28e4162981a6042f95d61d3.json"}}, {"family": "Inal", "given": "Sahika", "initials": "S", "orcid": "0000-0002-1166-1512", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c4303b98c3b64085a603b3c7fc222000.json"}}, {"family": "Yue", "given": "Wan", "initials": "W", "orcid": "0000-0003-4170-8614", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4c72a4ef1252410aa0e44134cd3a9c9d.json"}}], "type": "journal-article", "published": "2023-01-24", "journal": {"title": "Chem Mater", "issn": "0897-4756", "volume": "35", "issue": "2", "pages": "405-415", "issn-l": null}, "abstract": null, "doi": "10.1021/acs.chemmater.2c02447", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T08:09:07.610Z", "modified": "2026-08-20T08:09:07.853Z"}, {"entity": "publication", "iuid": "849a8ded05594d20977cfe01243da26a", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/849a8ded05594d20977cfe01243da26a.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/849a8ded05594d20977cfe01243da26a"}}, "title": "Astrocyte 3D Culture and Bioprinting using Peptide Functionalized Hyaluronan Hydrogels", "authors": [{"family": "Matthiesen", "given": "Isabelle", "initials": "I", "orcid": "0000-0003-4787-7785", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f57ebf85ca0d4d279b4852fd66f05251.json"}}, {"family": "Jury", "given": "Michael", "initials": "M", "orcid": "0000-0003-4079-4282", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4527b2ae00a94fcb80c1abb0abac64bb.json"}}, {"family": "Boroojeni", "given": "Fatemeh Rasti", "initials": "FR", "orcid": "0000-0002-3346-4755", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/71244257d00b4117b1b7e67b4ebba989.json"}}, {"family": "Ludwig", "given": "Saskia L", "initials": "SL"}, {"family": "Holzreuter", "given": "Muriel", "initials": "M", "orcid": "0000-0002-7580-3049", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/07143f4b91674a28a565da46ed4441f4.json"}}, {"family": "Buchmann", "given": "Sebastian", "initials": "S", "orcid": "0000-0001-7442-3020", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5d7fa19b55964898b15740e469f423a8.json"}}, {"family": "Tr\u00e4ger", "given": "Andrea", "initials": "A", "orcid": "0000-0001-6180-8917", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5de952fb4f584849a7567537d861de0a.json"}}, {"family": "Seleg\u00e5rd", "given": "Robert", "initials": "R", "orcid": "0000-0002-1781-1489", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1cb5b515710f4a77ab1fa32005577093.json"}}, {"family": "Winkler", "given": "Thomas E", "initials": "TE", "orcid": "0000-0002-2331-4833", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/851879d753574f5a80716e94dcc24590.json"}}, {"family": "Aili", "given": "Daniel", "initials": "D", "orcid": "0000-0002-7001-9415", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3624d34bc7734e329c0d4b465b5ac567.json"}}, {"family": "Herland", "given": "Anna", "initials": "A", "orcid": "0000-0002-5002-2537", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/367ed0d139fe4136808b62ee61baa4f0.json"}}], "type": "posted-content", "published": "2022-10-15", "journal": {"issn-l": null}, "abstract": null, "doi": "10.1101/2022.10.13.510969", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T10:41:37.557Z", "modified": "2026-08-20T10:41:37.823Z"}, {"entity": "publication", "iuid": "71734534a2814d04b69dd990d234cdc9", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/71734534a2814d04b69dd990d234cdc9.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/71734534a2814d04b69dd990d234cdc9"}}, "title": "Metabolic Assessment of Human Induced Pluripotent Stem Cells-Derived Astrocytes and Fetal Primary Astrocytes: Lactate and Glucose Turnover.", "authors": [{"family": "Matthiesen", "given": "Isabelle", "initials": "I"}, {"family": "Nasiri", "given": "Rohollah", "initials": "R", "orcid": "0000-0002-8245-692X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/aaecdaea25fc438e937adfea37188ae1.json"}}, {"family": "Tamashiro Orrego", "given": "Alessandra", "initials": "A"}, {"family": "Winkler", "given": "Thomas E", "initials": "TE", "orcid": "0000-0002-2331-4833", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/851879d753574f5a80716e94dcc24590.json"}}, {"family": "Herland", "given": "Anna", "initials": "A", "orcid": "0000-0002-5002-2537", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/367ed0d139fe4136808b62ee61baa4f0.json"}}], "type": "journal article", "published": "2022-10-08", "journal": {"title": "Biosensors (Basel)", "issn": "2079-6374", "volume": "12", "issue": "10", "issn-l": null}, "abstract": "Astrocytes represent one of the main cell types in the brain and play a crucial role in brain functions, including supplying the energy demand for neurons. Moreover, they are important regulators of metabolite levels. Glucose uptake and lactate production are some of the main observable metabolic actions of astrocytes. To gain insight into these processes, it is essential to establish scalable and functional sources for in vitro studies of astrocytes. In this study, we compared the metabolic turnover of glucose and lactate in astrocytes derived from human induced pluripotent stem cell (hiPSC)-derived Astrocytes (hiAstrocytes) as a scalable astrocyte source to human fetal astrocytes (HFAs). Using a user-friendly, commercial flow-based biosensor, we could verify that hiAstrocytes are as glycogenic as their fetal counterparts, but their normalized metabolic turnover is lower. Specifically, under identical culture conditions in a defined media, HFAs have 2.3 times higher levels of lactate production compared to hiAstrocytes. In terms of glucose, HFAs have 2.1 times higher consumption levels than hiAstrocytes at 24 h. Still, as we describe their glycogenic phenotype, our study demonstrates the use of hiAstrocytes and flow-based biosensors for metabolic studies of astrocyte function.", "doi": "10.3390/bios12100839", "pmid": "36290976", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC9599592"}, {"db": "pii", "key": "bios12100839"}], "notes": [], "created": "2026-08-20T13:39:00.656Z", "modified": "2026-08-20T13:39:00.766Z"}, {"entity": "publication", "iuid": "a2f97bf123604ca9a77207cd06251dfd", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/a2f97bf123604ca9a77207cd06251dfd.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/a2f97bf123604ca9a77207cd06251dfd"}}, "title": "Bioorthogonally Cross-Linked Hyaluronan-Laminin Hydrogels for 3D Neuronal Cell Culture and Biofabrication.", "authors": [{"family": "Jury", "given": "Michael", "initials": "M", "orcid": "0000-0002-2803-2237", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/788e0c74b5874bf18585debca9bcae79.json"}}, {"family": "Matthiesen", "given": "Isabelle", "initials": "I", "orcid": "0000-0003-4787-7785", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f57ebf85ca0d4d279b4852fd66f05251.json"}}, {"family": "Rasti Boroojeni", "given": "Fatemeh", "initials": "F", "orcid": "0000-0002-3346-4755", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/71244257d00b4117b1b7e67b4ebba989.json"}}, {"family": "Ludwig", "given": "Saskia L", "initials": "SL"}, {"family": "Civitelli", "given": "Livia", "initials": "L", "orcid": "0000-0003-2399-1436", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5515527ad9034e36ada5ef9f98007d01.json"}}, {"family": "Winkler", "given": "Thomas E", "initials": "TE", "orcid": "0000-0002-2331-4833", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/851879d753574f5a80716e94dcc24590.json"}}, {"family": "Seleg\u00e5rd", "given": "Robert", "initials": "R", "orcid": "0000-0002-1781-1489", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1cb5b515710f4a77ab1fa32005577093.json"}}, {"family": "Herland", "given": "Anna", "initials": "A", "orcid": "0000-0002-5002-2537", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/367ed0d139fe4136808b62ee61baa4f0.json"}}, {"family": "Aili", "given": "Daniel", "initials": "D", "orcid": "0000-0002-7001-9415", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3624d34bc7734e329c0d4b465b5ac567.json"}}], "type": "journal article", "published": "2022-06-00", "journal": {"title": "Adv Healthc Mater", "issn": "2192-2659", "volume": "11", "issue": "11", "pages": "e2102097", "issn-l": null}, "abstract": "Laminins (LNs) are key components in the extracellular matrix of neuronal tissues in the developing brain and neural stem cell niches. LN-presenting hydrogels can provide a biologically relevant matrix for the 3D culture of neurons toward development of advanced tissue models and cell-based therapies for the treatment of neurological disorders. Biologically derived hydrogels are rich in fragmented LN and are poorly defined concerning composition, which hampers clinical translation. Engineered hydrogels require elaborate and often cytotoxic chemistries for cross-linking and LN conjugation and provide limited possibilities to tailor the properties of the materials. Here a modular hydrogel system for neural 3D cell cultures, based on hyaluronan and poly(ethylene glycol), that is cross-linked and functionalized with human recombinant LN-521 using bioorthogonal copper-free click chemistry, is shown. Encapsulated human neuroblastoma cells demonstrate high viability and grow into spheroids. Long-term neuroepithelial stem cells (lt-NES) cultured in the hydrogels can undergo spontaneous differentiation to neural fate and demonstrate significantly higher viability than cells cultured without LN. The hydrogels further support the structural integrity of 3D bioprinted structures and maintain high viability of bioprinted and syringe extruded lt-NES, which can facilitate biofabrication and development of cell-based therapies.", "doi": "10.1002/adhm.202102097", "pmid": "35114074", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC11468931"}], "notes": [], "created": "2026-08-20T06:27:49.375Z", "modified": "2026-08-20T06:27:49.762Z"}, {"entity": "publication", "iuid": "33f710b9a5f849e2a369165c234cdc6c", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/33f710b9a5f849e2a369165c234cdc6c.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/33f710b9a5f849e2a369165c234cdc6c"}}, "title": "Advanced Materials and Sensors for Microphysiological Systems: Focus on Electronic and Electrooptical Interfaces.", "authors": [{"family": "Kavand", "given": "Hanie", "initials": "H", "orcid": "0000-0002-9144-0065", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b084b9302f774b03ab553d66d4fbbc66.json"}}, {"family": "Nasiri", "given": "Rohollah", "initials": "R", "orcid": "0000-0002-8245-692X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/aaecdaea25fc438e937adfea37188ae1.json"}}, {"family": "Herland", "given": "Anna", "initials": "A", "orcid": "0000-0002-5002-2537", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/367ed0d139fe4136808b62ee61baa4f0.json"}}], "type": "journal article", "published": "2022-04-00", "journal": {"title": "Adv. Mater. Weinheim", "issn": "1521-4095", "volume": "34", "issue": "17", "pages": "e2107876", "issn-l": "0935-9648"}, "abstract": "Advanced in vitro cell culture systems or microphysiological systems (MPSs), including microfluidic organ-on-a-chip (OoC), are breakthrough technologies in biomedicine. These systems recapitulate features of human tissues outside of the body. They are increasingly being used to study the functionality of different organs for applications such as drug evolutions, disease modeling, and precision medicine. Currently, developers and endpoint users of these in vitro models promote how they can replace animal models or even be a better ethically neutral and humanized alternative to study pathology, physiology, and pharmacology. Although reported models show a remarkable physiological structure and function compared to the conventional 2D cell culture, they are almost exclusively based on standard passive polymers or glass with none or minimal real-time stimuli and readout capacity. The next technology leap in reproducing in vivo-like functionality and real-time monitoring of tissue function could be realized with advanced functional materials and devices. This review describes the currently reported electronic and optical advanced materials for sensing and stimulation of MPS models. In addition, an overview of multi-sensing for Body-on-Chip platforms is given. Finally, one gives the perspective on how advanced functional materials could be integrated into in vitro systems to precisely mimic human physiology.", "doi": "10.1002/adma.202107876", "pmid": "34913206", "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T06:27:56.504Z", "modified": "2026-08-20T06:27:56.653Z"}]}