{"entity": "researcher", "timestamp": "2026-09-23T15:40:56.830Z", "family": "Flodstr\u00f6m Tullberg", "given": "Malin", "initials": "M", "orcid": "0000-0003-2685-2052", "affiliations": ["Center for Infectious Medicine, Department of Medicine Huddinge, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/358926667b494e6d8cc544f34bc80c5e.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/358926667b494e6d8cc544f34bc80c5e"}}, "publications": [{"entity": "publication", "iuid": "c46864c055a2410f934204be0ea3342e", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/c46864c055a2410f934204be0ea3342e.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/c46864c055a2410f934204be0ea3342e"}}, "title": "Frequent longitudinal blood microsampling and proteome monitoring identify disease markers and enable timely intervention in a mouse model of type 1 diabetes.", "authors": [{"family": "Parajuli", "given": "Anirudra", "initials": "A", "orcid": "0000-0001-6886-0566", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/26fcf5ba1d1f44729e29483afd2ab30f.json"}}, {"family": "Bendes", "given": "Annika", "initials": "A", "orcid": "0000-0001-9329-2353", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b84a8aa3e3b4439bb0b3a9fabdebcb70.json"}}, {"family": "Byvald", "given": "Fabian", "initials": "F", "orcid": "0000-0003-0516-5448", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0b970057f7774228b303ea223f2ca0f4.json"}}, {"family": "Stone", "given": "Virginia M", "initials": "VM", "orcid": "0000-0003-3091-2142", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/683fdb74b2ca4db2ae812b91f67aee71.json"}}, {"family": "Ringqvist", "given": "Emma E", "initials": "EE", "orcid": "0000-0001-6303-6076", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/7b69395e6574425fb9014eb3c0b0f465.json"}}, {"family": "Butrym", "given": "Marta", "initials": "M", "orcid": "0000-0002-9285-7276", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c7ed9ae51b874127b7ea5a65a9bfa837.json"}}, {"family": "Angelis", "given": "Emmanouil", "initials": "E"}, {"family": "Kipper", "given": "Sophie", "initials": "S", "orcid": "0009-0004-8075-1660", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a5d869e26fe54420a014f8a03e49e836.json"}}, {"family": "Bauer", "given": "Stefan", "initials": "S", "orcid": "0000-0003-1712-060X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/36b40da583c14c4e96684d2df87e8840.json"}}, {"family": "Roxhed", "given": "Niclas", "initials": "N", "orcid": "0000-0002-7147-6730", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/479a5ae1d69b42859bce8537851586ce.json"}}, {"family": "Schwenk", "given": "Jochen M", "initials": "JM", "orcid": "0000-0001-8141-8449", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/022e140799b6466ea123319c3d70c76e.json"}}, {"family": "Flodstr\u00f6m-Tullberg", "given": "Malin", "initials": "M", "orcid": "0000-0003-2685-2052", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/358926667b494e6d8cc544f34bc80c5e.json"}}], "type": "journal article", "published": "2025-10-00", "journal": {"title": "Diabetologia", "issn": "1432-0428", "volume": "68", "issue": "10", "pages": "2277-2289", "issn-l": "0012-186X"}, "abstract": "Type 1 diabetes manifests after irreversible beta cell damage, highlighting the crucial need for markers of the presymptomatic phase to enable early and effective interventions. Current efforts to identify molecular markers of disease-triggering events lack resolution and convenience. Analysing frequently self-collected dried blood spots (DBS) could enable the detection of early disease-predictive markers and facilitate tailored interventions. Here, we present a novel strategy for monitoring transient molecular changes induced by environmental triggers that enable timely disease interception.\n\nWhole blood (10 \u03bcl) was sampled regularly (every 1-5 days) from adult NOD mice infected with Coxsackievirus B3 (CVB3) or treated with vehicle alone. Blood samples (5 \u03bcl) were dried on filter discs. DBS samples were analysed by proximity extension assay. Generalised additive models were used to assess linear and non-linear relationships between protein levels and the number of days post infection (p.i.). A multi-layer perceptron (MLP) classifier was developed to predict infection status. CVB3-infected SOCS-1-transgenic (tg) mice were treated with immune- or non-immune sera on days 2 and 3 p.i., followed by monitoring of diabetes development.\n\nFrequent blood sampling and longitudinal measurement of the blood proteome revealed transient molecular changes in virus-infected animals that would have been missed with less frequent sampling. The MLP classifier predicted infection status after day 2 p.i. with over 90% accuracy. Treatment with immune sera on day 2 p.i. prevented diabetes development in all (100%) of CVB3-infected SOCS-1-tg NOD mice while five out of eight (62.5%) of the CVB3-infected controls treated with non-immune sera developed diabetes.\n\nOur study demonstrates the utility of frequently collected DBS samples to monitor dynamic proteome changes induced by an environmental trigger during the presymptomatic phase of type 1 diabetes. This approach enables disease interception and can be translated into human initiatives, offering a new method for early detection and intervention in type 1 diabetes.\n\nAdditional data available at https://doi.org/10.17044/scilifelab.27368322 . Additional visualisations are presented in the Shiny app interface https://mouse-dbs-profiling.serve.scilifelab.se/ .", "doi": "10.1007/s00125-025-06502-7", "pmid": "40760251", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC12423192"}, {"db": "pii", "key": "10.1007/s00125-025-06502-7"}], "notes": [], "created": "2026-09-23T12:33:10.913Z", "modified": "2026-09-23T12:33:11.284Z"}, {"entity": "publication", "iuid": "3a3a8ff693ed4aa88f61e02c39248a15", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/3a3a8ff693ed4aa88f61e02c39248a15.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/3a3a8ff693ed4aa88f61e02c39248a15"}}, "title": "Intestinal stroma guides monocyte differentiation to macrophages through GM-CSF.", "authors": [{"family": "Kvedaraite", "given": "Egle", "initials": "E", "orcid": "0000-0001-5308-092X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/12e2bb98f9ea4186a3fc75703b205484.json"}}, {"family": "Lourda", "given": "Magda", "initials": "M", "orcid": "0000-0003-3155-1123", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c3b0f1d58b48430b8553aceb99998d40.json"}}, {"family": "Mouratidou", "given": "Natalia", "initials": "N"}, {"family": "D\u00fcking", "given": "Tim", "initials": "T", "orcid": "0000-0002-4347-7648", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5c4b1032e7a34102af444c74bf67dd59.json"}}, {"family": "Padhi", "given": "Avinash", "initials": "A"}, {"family": "Moll", "given": "Kirsten", "initials": "K"}, {"family": "Czarnewski", "given": "Paulo", "initials": "P"}, {"family": "Sinha", "given": "Indranil", "initials": "I", "orcid": "0000-0002-2513-5927", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/83272c13da5c4ea6a8154656d53efb00.json"}}, {"family": "Xagoraris", "given": "Ioanna", "initials": "I"}, {"family": "Kokkinou", "given": "Efthymia", "initials": "E"}, {"family": "Damdimopoulos", "given": "Anastasios", "initials": "A"}, {"family": "Weigel", "given": "Whitney", "initials": "W"}, {"family": "Hartwig", "given": "Olga", "initials": "O", "orcid": "0000-0001-5016-5960", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1cbc793bf0f147558140e652a8d2cfa2.json"}}, {"family": "Santos", "given": "Telma E", "initials": "TE"}, {"family": "Soini", "given": "Tea", "initials": "T"}, {"family": "Van Acker", "given": "Aline", "initials": "A"}, {"family": "Rahkonen", "given": "Nelly", "initials": "N"}, {"family": "Flodstr\u00f6m Tullberg", "given": "Malin", "initials": "M", "orcid": "0000-0003-2685-2052", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/358926667b494e6d8cc544f34bc80c5e.json"}}, {"family": "Ringqvist", "given": "Emma", "initials": "E"}, {"family": "Buggert", "given": "Marcus", "initials": "M", "orcid": "0000-0003-0633-1719", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/cf1bb88eef764f8bb81bdd6d8a231113.json"}}, {"family": "Jorns", "given": "Carl", "initials": "C", "orcid": "0000-0001-7727-8113", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ef68cb234b3141278a29a407ed988b32.json"}}, {"family": "Lindforss", "given": "Ulrik", "initials": "U"}, {"family": "Nordenvall", "given": "Caroline", "initials": "C"}, {"family": "Stamper", "given": "Christopher T", "initials": "CT"}, {"family": "Unnersj\u00f6-Jess", "given": "David", "initials": "D", "orcid": "0000-0002-4162-0973", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/32d1982020d14121b558c26285b212ca.json"}}, {"family": "Akber", "given": "Mira", "initials": "M"}, {"family": "Nadisauskaite", "given": "Ruta", "initials": "R"}, {"family": "Jansson", "given": "Jessica", "initials": "J"}, {"family": "Vandamme", "given": "Niels", "initials": "N"}, {"family": "Sorini", "given": "Chiara", "initials": "C", "orcid": "0000-0002-6803-8377", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/652b92b02a9448179fb0bf7648ed30b9.json"}}, {"family": "Grundeken", "given": "Marijke Elise", "initials": "ME", "orcid": "0000-0001-6915-0339", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/79de930c618c42bbae8e3c7729373d11.json"}}, {"family": "Rolandsdotter", "given": "Helena", "initials": "H"}, {"family": "Rassidakis", "given": "George", "initials": "G"}, {"family": "Villablanca", "given": "Eduardo J", "initials": "EJ", "orcid": "0000-0001-9522-9729", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c90ee0c3d1904ae0933efe3631a651e0.json"}}, {"family": "Idestr\u00f6m", "given": "Maja", "initials": "M"}, {"family": "Eulitz", "given": "Stefan", "initials": "S"}, {"family": "Arnell", "given": "Henrik", "initials": "H"}, {"family": "Mj\u00f6sberg", "given": "Jenny", "initials": "J", "orcid": "0000-0002-1119-0976", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/33e4f7ea779c4b3382604de7ea259bc8.json"}}, {"family": "Henter", "given": "Jan-Inge", "initials": "JI", "orcid": "0000-0002-0629-2126", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c03e0a2905534e26b5a2a2b5de453872.json"}}, {"family": "Svensson", "given": "Mattias", "initials": "M", "orcid": "0000-0003-1695-7934", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f3cb4fb47f3c4be0a6d529a4d8382b82.json"}}], "type": "journal article", "published": "2024-02-26", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "15", "issue": "1", "pages": "1752", "issn-l": "2041-1723"}, "abstract": "Stromal cells support epithelial cell and immune cell homeostasis and play an important role in inflammatory bowel disease (IBD) pathogenesis. Here, we quantify the stromal response to inflammation in pediatric IBD and reveal subset-specific inflammatory responses across colon segments and intestinal layers. Using data from a murine dynamic gut injury model and human ex vivo transcriptomic, protein and spatial analyses, we report that PDGFRA+CD142-/low fibroblasts and monocytes/macrophages co-localize in the intestine. In primary human fibroblast-monocyte co-cultures, intestinal PDGFRA+CD142-/low fibroblasts foster monocyte transition to CCR2+CD206+ macrophages through granulocyte-macrophage colony-stimulating factor (GM-CSF). Monocyte-derived CCR2+CD206+ cells from co-cultures have a phenotype similar to intestinal CCR2+CD206+ macrophages from newly diagnosed pediatric IBD patients, with high levels of PD-L1 and low levels of GM-CSF receptor. The study describes subset-specific changes in stromal responses to inflammation and suggests that the intestinal stroma guides intestinal macrophage differentiation.", "doi": "10.1038/s41467-024-46076-3", "pmid": "38409190", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC10897309"}, {"db": "pii", "key": "10.1038/s41467-024-46076-3"}], "notes": [], "created": "2026-09-23T09:04:49.642Z", "modified": "2026-09-23T09:04:50.297Z"}]}